PRACTICAL

MICROBIOLOGYSterilization, Disinfection , and Antisepsis.

The use of Microscope.

Destroying Microorganism

Sterilization Disinfection Antiseptics

Sterilization

Is the removal of all microbes, including endosores.

Can be achieved by mechanical means, heat , chemicals

or radiation.

Monitoring steam sterilization

• Is the process of eliminates most or all microorganisms,

with the exception of Endospores.

Disinfection

Disinfection

High level disinfectants

Intermediate level

disinfectants

Low level disinfectants

• Is the process of destroying or inhibiting the growth of

microorganisms in or on living tissue and can also be

referred as biocides.

Antiseptics

• Mechanical methods such as scrubbing with a brush are

most commonly, but alternatively alcohol-based rubs are

also used for hand disinfection.

Disinfection of Hands

The use of Microscope

The oil immersion objective

Oil and glass have the same refractive

index