microbiology (non majors) lab practical 1 key terms flash cards
TRANSCRIPT
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Microbiology (non majors)Lab Practical I Key Terms
1a
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PRINT INSTRUCTIONS: place 3X5flash cards in printer and set printerto print custom size (3x5) be sure toselect duplex printing. Once thefronts are printed simply flip stackand place back in printer to print onback of cards.
1b
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AEROBIC
2a
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requires oxygen (oppositeof anaerobic)
2b
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AGAR
3a
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powder added to media forsolidification
3b
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AIR-DRY
4a
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drying of slide suspensionin air before heat fixing and
staining
4b
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AGGLUTINATION
5a
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the reaction betweenantigen and specific
antibody
5b
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ALIQUOT
6a
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dispense an amount ofliquid using a pipette
6b
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ANALOG
7a
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similar structure, but notidentical
7b
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ANTIBODY
8a
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specific, protective proteinproduced by the immunesystem in response to an
antigen8b
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ANTIGEN
9a
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foreign, non-selfimmunogenic material thatelicits an immune response
9b
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ASEPTIC TECHNIQUE
10a
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procedure to guaranteesterility and to reduce
contamination
10b
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ATRICHOUS
11a
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without flagella, nonmotile
11b
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AUTOCLAVE
12a
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moist heat method ofsterilization using pressure
12b
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AXIAL FILAMENT
13a
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a structure for motilityused by the Spirochaete
bacteria
13b
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BIBULOUS PAPER
14a
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absorbant paper used toblot dry slides after
staining
14b
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BHI
15a
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brain heart infusion, areally good enrichment
medium
15b
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BRIGHTFIELDMICROSCOPY
16a
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full light directed towardsthe specimen, used for
stained and live specimens
16b
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BROTH
17a
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medium without agar
17b
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BROWNIAN MOVEMENT
18a
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vibrations of an object seenin a microscope, not true
motility
18b
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CANDLE JAR
19a
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candle burns in a closedcontainer producing a carbondioxide incubator, containing
2-10% O 2 and around 10% CO 2
19b
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CFU
20a
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colony-forming units, i.e.colonies
20b
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CHEMICALLY-DEFINEDMEDIUM
21a
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a synthetic medium,comprised of known
ingredients and knownamounts
21b
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CHITIN
22a
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a complex polysaccharidemolecule found in walls offungi and exoskeletons of
shrimps and crabs22b
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CNA
23a
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Columbia naladixic acidmedium, selective (for
Gram +) and differentialmedium
23b
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COAGULATION
24a
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clotting of blood, plasma
24b
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COLIFORMS
25a
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gram - rods which fermentlactose, nonsporeforming
25b
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COLONY
26a
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a visible mass of bacteriagrowing on solidifed
medium, a clone
26b
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COMPLEX MEDIUM
27a
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medium with someunknown ingredients oramounts, i.e. blood agar
27b
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CONDENSER
28a
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collects all available lightrays for direction up to the
stage opening
28b
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COUNTERSTAIN
29a
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the 2nd dye added to asmear, taken in after thewall is decolorized, e.g.
safrinin,methylene blue
29b
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DARKFIELDMICROSCOPY
30a
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special condenser blocks mostlight rays, only reflected lightbounces off specimeninto lens producing a black fieldof vision; used for wet mounts
30b
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DECOLORIZER
31a
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the reagent used to removethe primary dye from the cellwall in a differential stain,e.g. acid alcohol, acetone-alcohol
31b
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DIFFERENTIAL STAIN
32a
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uses 2 or more dyes whichallow differentiation
between different bacterialgroups orstructures
32b
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DIAPHRAGM
33a
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acts as an iris within thecondenser which opens and
closes for sensitive lightcontrol
33b
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DISACCHARIDE
34a
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2 sugar molecule, e.g.lactose or sucrose
34b
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EMB
35a
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Eosin methylene bluemedium, selective (for
Gram -) and differentialmedium
35b
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EXOENZYM E
36a
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enzyme excreted away fromthe cell
36b
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FACULTATIVEANAEROBE
37a
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uses oxygen when presentbut can either ferment or
anaerobically respirewithout it
37b
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FASTIDIOUS
38a
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hard-to-grow bacteria,requiring grow factors or
particular nutrients
38b
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FECAL COLIFORMS
39a
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gram - rods which fermentlactose, nonsporeforming,
GI flora in animals, in feces
39b
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FLAGELLA
40a
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a structure for motility
40b
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FLAGELLATION
41a
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differential category offlagella placement around
the cell
41b
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GENUS
42a
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category of organisms withlike features and closely
related, divided into species
42b
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HALOPHILIC
43a
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salt-tolerant or salt-loving(salt-requiring)
43b
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HEAT-FIX
44a
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use of the flame to 1)coagulate proteins of the
suspension, causingadherence to
slide, and 2) kill the microbes44b
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HELMINTH
45a
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parasitic worm
45b
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HYPHA
46a
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A fungal filament (plural,hyphae)
46b
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IMMUNOASSAY
47a
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test which identifies antibodyin patient based on use of aknown antigen, or identifiesthe antigen based on the useof a known antibody
47b
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IMVIC
48a
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acronym = indole, methylred, Voges-Proskauer,
citrate
48b
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INOCULUM
49a
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a small sample of themicroorganism
49b
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MIC
50a
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minimal inhibitoryconcentration of antibioticthat inhibits a bacterium
50b
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MICROAEROPHILIC
51a
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likes a reduced oxygenconcentration
51b
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MONOSACCHARIDE
52a
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simple sugar, e.g. glucose
52b
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MYCELIUM
53a
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A visible mass of hyphalfilaments
53b
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NA/NB
54a
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nutrient agar or nutrientbroth
54b
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NONIONIC
55a
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no electrical charge
55b
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OBLIGATE AEROBE
56a
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requires oxygen to grow
56b
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OBLIGATE ANAEROBE
57a
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does not use oxygen togrow, may even be killed by
it
57b
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OIL-IMMERSION LENS
58a
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100X objective lens, to beused always with
immersion oil
58b
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PARFOCAL
59a
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feature of microscopewhich allows rotation oflenses with only minor
focus movement,lenses alignment
59b
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PATHOGENIC
60a
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disease-causing
60b
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PCA
61a
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plate count agar medium,general all-purpose
enrichment
61b
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PFU
62a
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plaque-forming unitsproduced by bacterial
viruses when infecting hostbacterial cells
62b
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PHASE-CONTRASTMICROSCOPY
63a
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special condenser below stageand in objectives change speedof light rays,enhancing density differencesinside and outside of cells; usedfor wet mounts
63b
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PHAGE TYPING
64a
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test to identify a bacteriumbased on which known
virus infects it
64b
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PHENOTYPE
65a
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expression of a gene as atrait
65b
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PLAQUE
66a
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destruction of the bacteriallawn by a bacteriophage as
the lytic infectionprogresses
66b
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PLATE COUNT AGAR
67a
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variation of nutrient agar,for optimizing counts of
bacteria in samples
67b
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POUR PLATE
68a
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procedure where liquifiedagar has been poured into apetri dish after being mixed
withbacteria
68b
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PRIMARY DYE
69a
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the 1st dye used in adifferential stain, e.g.
malachite green, crystalviolet, carbol
fuschin69b
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RESIDENT FLORA
70a
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Microbes firmly entrenchedin niches in/on the body
70b
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REVOLVING NOSEPIECE
71a
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rotating turret attached tothe 3 lenses
71b
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RESAZURIN
72a
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oxygen indicator inthioglycollate broth, pink
when oxygenated
72b
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SEROLOGY
73a
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the analysis of substances—antigen or antibody--in
blood serum
73b
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SPECIES
74a
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a subdivision of a genus,almost identical organisms,
a clone
74b
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SPREAD PLATE
75a
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procedure where pre-madeagar plates have a sample ofbacterium placed on top ofthe agar and spread via a
glass rod75b
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STREAK PLATE
76a
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procedure where a bacterialspecimen is placed on a pre-made plate and diluted outusing flame and multiple
sections.76b
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SYMBIOTIC/SYMBIOSIS
77a
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a relationship between 2 ormore organisms
77b
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THERMAL DEATH TIME
78a
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minimal amount of time tocompletely sterilize aspecimen at a certain
temperature78b
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TRANSIENT FLORA
79a
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Microbes easily dislodgedfrom the body, come-and-
go
79b
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TSB/TSA
80a
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trypticase soy broth ortrypticase soy agar
80b
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ZONE OF INHIBITION
81a
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area of no bacterial growtharound a chemical on a
disc, indicates sensitivity
81b