Conclusion: Our study demonstrates umbilical cord vascu-lar abnormalities in PE, FGR and PTB, suggesting early vascu-lar damage. Future studies should focus on similar structuralchanges in foetal vasculature and associations with cardio-vascular disease in later life.

doi:10.1016/j.preghy.2013.04.040

PP013. Single nucleotide polymorphisms of the maternalcystathionine-b-synthase gene are associated with pre-eclampsia (PE)Holwerda Kim, Weedon-Fekjær Susanne, Staff Annetine,

Nolte Ilja, Goor Harry van, Lely Titia, Faas Marijke

Introduction: Cystathionine-b-synthase (CBS) producesthe vasodilatory and anti-inflammatory hydrogen sulfide(H2S) by conversion of homocysteine (Hcy). H2S is involvedin placental vascular tone regulation. Previously, we showedthat woman with PE have hyperhomocysteinemia anddecreased placental CBS gene expression.

Objectives: Aberrant CBS gene expression may play a rolein hyperhomocysteinemia and decreased H2S and could beinvolved in pathogenesis of PE. We studied whether thepresence of CBS single nucleotide polymorphisms (SNPs) isassociated with the development of PE.

Methods: Six CBS SNPs (rs12329764, rs2851391,rs234713, rs234706, rs1789953, rs11203172) were geno-typed in 99 controls, 60 severe, and 39 mild PE cases. Severeand mild PE cases were additionally subdivided into late-(>34 weeks) and early-onset (<34 weeks) PE. The associationof the alleles with development PE was tested with logisticregression.

Results: Two of the six SNPs are associated with PE. Theminor allele for rs11203172 reduces the risk for developingsevere PE (OR[95% CI] = 0.54[0.21–0.94], p = 0.023). Theminor allele for rs234706, which is associated with lowHcy, increased the risk to develop mild, late-onset PE(2.10[1.15–3.85], p = 0.016).

Conclusion: SNPs in the CBS gene are associated with riskof developing PE. Within the CBS gene, SNPs associated withboth a decreased and an increased risk to develop PE were

found. Altered effectiveness of CBS may affect PE throughdecreased H2S production or Hcy accumulation.

doi:10.1016/j.preghy.2013.04.041

PP014. A pioneer explore of PTZ-induced eclampsia inpreeclampsia ratsHuang Qian, Liu Huishu, Hu Bihui, Ye Yuanjuan

Introduction: Eclampsia is the most serious state of hyper-tensive disorder in pregnancy, but the pathogenesis ofeclampsia seizures is poorly understood.

Objectives: An eclampsia animal model was developed byPTZ injection on preeclampsia rats.

Method: Rats received endotoxin (1.0 lg/kg body weight)form tail vein in an hour on GD14 to build the preeclampsia(PE) model. PTZ i.p injection were given to PE rats on GD16,17, 18 to induce seizures and build an eclampsia model. Allexperiment rats were divided into three groups, PE, normal,pregnant (P), non-pregnant (NP) group (n = 18 in each group).Each group gave two doses of PTZ (40 or 35 mg/kg) as sub-groups. Blood pressure, urinary albumin, biochemicalindexes (AST, ALT, SCr, Urea) were measured. Seizure sever-ity was defined by Racine’ standard and seizure time wererecorded.

Results: PTZ-induced eclampsia shows a increasing bloodpressure, urinary albumin, liver function damage and differentstages of seizures. The latency of seizure in PE rats with 40 mg/kg PTZ is obviously shortened when compared with the P andNP group (p < 0.05) and the duration is prolonged (p < 0.05).The same trend is still existence at the low dose of PTZ injec-tion. Rate of tonic-clonic seizures is elevated in PE and Pgroup when compared with NP group, but nonsignificantly.

Conclusion: We use 40 mg/kg PTZ to endotoxin inducedpreeclampsia model to build an eclampsia model, whichmimicked the multiple organ function disorder in humaneclampsia. Meanwhile, we found the PE status maydecreases threshold for PTZ induced-seizures.

doi:10.1016/j.preghy.2013.04.042

PP012. Table 1. Umbilical cord morphology.

PE (n = 31) FGR (n = 26) PTB (n = 24) Controls (n = 24) Overall P-value

Macroscopy

Length (cm) 43.25⁄ 50.09 46.08 50.94 P = 0.006

(19–75) (27–69) (20–64) (24–86)

Microscopy

Total cord area (mm2) 74.06⁄ 68.65⁄ 85.88⁄ 112.63 P < 0.001

(37.84–131.21) (31.87–145.35) (55.80–109.69) (64.89–182.60)

Vein wall area (mm2) 4.39⁄ 5.55 6.80 7.20 P = 0.020

(1.66–17.43) (1.63–10.45) (2.36–13.69) (4.27–13.12)

Artery wall area (mm2) 2.29⁄ 2.52⁄ 3.11 3.46 P < 0.001

(1.62–3.44) (1.62–3.79) (1.93–5.69) (2.45–5.26)

Vein elastic fibres (%) 7.18⁄ 7.32⁄ 7.53 8.99 P = 0.022

(2.88–13.85) (4.16–12.37) (4.85–16.23) (5.59–17.41)

ANOVA, mean (range).⁄ P < 0.05 compared to controls.

72 Poster Presentations / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 3 (2013) 67–99