No-cross reactive Abs

Solid-phase absorption column

Cross reactive Abs

Mouse or Rat serum protein

Absorption stepsAbsorption steps

ImmunohistochemistryIHC

-Histofine®

Rat MAX PO Mouse MAX PO

Only no-cross reactive secondary antibodies are obtained.

Use the Fab’ flagment as Secondary Abs

1. Before absorption of secondary antibody. 4. Get no-cross reactive secondary antibodies.

5. Enzyme digestion to Fab’2. Through the column.

3. Capture the cross reactive antibodies.

No-cross reaction with Rat & Mouse tissues sections

Solid-phase absorption with Rat & Mouse serum protein

Mouse Rat

Rat & Mouse tissues sectionsPolymer Detection system for

Liquid. Ready to use.

-Histofine® Simple Stain AEC Solution 415182F415184F

3 x 17ml9 x 17ml

3 x 3ml3 x 9ml

Peroxidase

Peroxidase

-Histofine®

-Histofine®

-Histofine®

Simple Stain Rat MAX PO (MULTI)

Simple Stain Rat MAX PO (M)

Simple Stain Rat MAX PO (G)

170 414191F

170 414171F

170 414331F

Mouse and rabbitprimary antibodies

Mouse primary antibody

Goatprimary antibody

1 x 17ml

1 x 17ml

-Histofine® Simple Stain Rat MAX PO (R) 170 414181F Rabbit primary antibody1 x 17ml

1 x 17ml

-Histofine®

・No Cross-reaction with Rat & Mouse tissue sections・Higher Sensitivity・One-step Polymer = Skip staining steps・Lower background staining

-Histofine®

-Histofine®Simple Stain Mouse MAX PO (Rat)

Simple Stain Mouse MAX PO (R)

Simple Stain Mouse MAX PO (G)

170

414311F

170

414341F

170

414351F

Rat primary antibodies

Rabbit primary antibody

Goatprimary antibody

1 x 17ml

1 x 17ml

1 x 17ml

Enzyme Substrate Systems

For Rat Tissue Sections

For Mouse Tissue Sections

Mark in EU500

1500DAB-3S kitReagent A : DAB solution concentrate Reagent B : Enhancer solution concentrateReagent C : Hydrogen peroxide solution concentrate

415192F415194F-Histofine®

6-19-20, TSUKIJI, CHUO-KU,TOKYO, 104-8402, JAPANPHONE: 81-3-3248-2207FAX: 81-3-3248-2243http://www.nichirei.co.jp/bio/english 2011.07

Add primary antibody. Add polymer. Add substrate solution.

Antigen

Rat or Mouse tissue section

Countersta

ining

Countersta

ining

Mounting

Mounting

-Histofine®

Peroxidase Amino-acid polymer

Fab' fragment ofsecondary antibody

Simple Stain Rat or Mouse MAX PO

30 min.

Principle & ProcedurePrinciple & Procedure

Product ListProduct List

■Rat anti-Mouse CD45R/B220

Mouse spleen stained with -Histofine® Simple Stain Mouse MAX PO (Rat) and DAB chromogen. Note membrane staining of almost all lymphocytes in germinal center and scattered interfollicular lymphocytes.

■Rabbit anti-Keratin/Cytokeratin antibody

Mouse skin stained with -Histofine® Simple Stain Mouse MAX PO(R) and DAB chromogen.Note cytoplasmic staining of epithelium cells and sweat gland cells.

Rat liver stained with -Histofine® Simple Stain Rat MAX PO (MULTI) and DAB chromogen. Note nuclear staining of liver cells.

■Mouse anti-PCNA antibody (clone:PC10)

AdvantagesAdvantages