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ERASMUS+ PROGRAMMEMaria Curie-Sklodowska University,

Lublin, PolandErasmus code: PL LUBLIN01

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STUDY PROGRAMME

1.Course title: Basic and Medical Virology

Course code: Faculty/Department: Faculty of Biology and Biotechnology, Department of Virology and ImmunologyTeacher: Agnieszka Szuster-Ciesielska, Ph.D., Associate Professor ([email protected])

(+48) 81 537 59 43 Magdalena Mizerska-Dudka, Ph.D. ([email protected]) Lecture/Laboratory, 30 hrs/30 hrs ECTS credits: 5Year of study: I (II0) winter semester

Educational and professional goals As a result of the course the student should be able to:Explain the origin, structure, replication of viruses, host-virus interactions as well as methods of fighting viral infections..Characterize of viruses families pathogenic for humans. Indicate the way of prophylaxis against viruses. Use the biological models for propagation and isolation of viruses. Work with cell cultures. Apply cell cultures to propagation and titration of viruses. Conducting according the ethical principles and work safety regulations. Awareness of viral diseases risk. Care of the health-oriented personal behavior.

Course description Lecture contents: General virology: definitions, structure of viruses, classification, replication, virulence, mechanisms of cell injury, acute and persistent infections, viral evolution, host-virus interactions. Characterization of main families of DNA and RNA viruses pathogenic for humans. DNA and RNA tumor viruses. Emerging viruses and zoonosis. Vaccines and antivirals. Laboratory exercise contents: Organization of BSL-2 laboratory. Principles of working with infectious material. Propagation and isolation of viruses in laboratory animals and chick embryos. Detection and titration of propagated viruses in biological materials. Primary cell culture - isolation and maintenance of chick embryo fibroblasts. Manipulation of cultured cells - primary and continuous cell lines; tissue bank - freezing and thawing cell cultures. Propagation of viruses in cell cultures. Viral cytophatic effect. Virus titration and storage methods. Literature:1. „Human and Medical Virology” ed. B.W.J. Mahy (2010) 2. „Clinical Virology” ed. A.J. Zuckerman (2009)3. "Viruses" D.R.Harper (2012)

Course type: lecture and practical laboratoryForm of assessment: final written exam, continuous assessment of laboratoriesPrerequisities: completed biochemistry and microbiology course. Primary target group: students of biology, biotechnology2.Course title: Immunology with elements of virology

mailto:[email protected]


(+48) 81 537 59 43 Magdalena Mizerska-Dudka, Ph.D. ([email protected]) Lecture/Laboratory: 30 hrs/45 hrs ECTS credits: 6.5 Year of study: III (I0), winter semester

Educational and professional goals The course provides a basic theoretical and technical study of mechanisms of the immune response, immunodiagnostics, characterization of main families of DNA and RNA viruses pathogenic for humans. Upon completion of the course, the student should be able to: explain mechanisms involved in physiological and pathological immune reactions, define the basic immunological test used in practice, explain the structure, and replication of viruses, characterize of viruses families pathogenic for humans, indicate the way of prophylaxis against viruses.

Course description

Lecture contents: Development of central and peripheral immune system. Innate immune system: function, anatomical barriers, inflammation, complement system, phagocytosis. Adaptive immune system: function, structure and characteristic of antigens, and antibodies, antigen presentation, humoral and cellular immune response. General virology: structure of viruses, replication. Characterization of main families of DNA and RNA viruses pathogenic for humans. Laboratory exercise contents: Methods of study innate and adaptive immunological response. Central and peripheral immune system, isolation of cells from lymphatic organs. Phagocytosis determination. Proliferation and differentiation of lymphocytes. Macrophage migration inhibition test. Some techniques used in immunodiagnosis (ELISA, Latex tests). Isolation of viruses propagated in laboratory animals and chick embryos. Maintenance of continuous cell lines. Propagation of viruses in cell cultures. Virus titration methods.Literature:

1. "Roitt's Essential Immunology" P.J.Delves, S.J.Martin, D.R.Burton, I.M.Roitt (2011)2. "Human and Medical Virology" ed. B.W.J. Mahy (2010)3. "Viruses" D.R.Harper (2012) 4. "Clinical Virology" ed. A.J. Zuckerman (2009)

Course type: lecture and practical laboratoryForm of assessment: final written exam, continuous assessment of laboratoriesPrerequisities: completed biochemistry and microbiology course. Primary target group: students of biology, biotechnology


3.Course title: Virology basic course


(+48) 81 537 59 43 Magdalena Mizerska-Dudka, Ph.D. ([email protected]) Lecture/Laboratory, 15 hrs/30 hrs ECTS credits: 4Year of study: I (II0) summer semester

Educational and professional goals Knowledge: As a result of the course the student should be able to:

1. Explain the structure, and replication of viruses, as well as host cells attack methods.2. Characterize of viruses families pathogenic for humans. 3. Indicate the way of prophylaxis against viruses.

Skills: As a result of the course the student should be able to:1. Use the biological models for the isolation of viruses.2. Work with cell cultures. 3. Apply cell cultures to propagation and titration of viruses.

Attitude: As a result of the course the student will acquire the following attitudes:1. Conducting according the ethical principles and work safety regulations. 2. Awareness of viral diseases risk. 3. Care of the health-oriented personal behavior.

Course description

Lecture contents: General virology (definitions, structure of viruses, classification, replication). Characterization of main families of DNA and RNA viruses pathogenic for humans. Laboratory exercise contents: Isolation of viruses propagated in laboratory animals and chick embryos. Maintenance of continuous cell lines; tissue bank - the freezing and thawing the culture. Propagation of viruses in cell cultures. Virus titration methods.Literature:4. "Human and Medical Virology" ed. B.W.J. Mahy (2010) 5. "Clinical Virology" ed. A.J. Zuckerman (2009)6. "Viruses" D.R.Harper (2012)



4.Course title: Biochemistry of Secondary Metabolites

Faculty/Department: Biochemistry Department, Faculty of Biology and Biotechnology Course code: Erasmus subject area code: Number of contact hours: 30 hours – lecture; 30 hours - laboratory Course duration: I 20, summer semesterECTS credits: 5 Course description: The position of secondary metabolism in biochemical processes in living organisms and its regulation at molecular and environmental level. The course covers the major groups of secondary metabolites in microorganisms and plants, from the perspectives of biochemistry and biosynthesis with references to the relationship between biological function of secondary metabolites in defense against different stress. Characteristics of basic secondary metabolites (bacterial, fungal and from plants), their biosynthetic pathways and practical applications for example such as pharmacological compounds. Literature: Dewick PM “Medicinal natural products. A biosynthetic approach.” John Wiley & Sons, LTD, 2002Wink M (ed) “Functions and biotechnology of plant secondary metabolites” Wiley-Blackwell, 2010Course type: lecture and practical laboratory Assesment method: written examination, continuous assessment of laboratories Prerequisities: completed course in biochemistry Primary target group: students of biology, biotechnology, organic chemistry Lecturer: dr hab. Anna Jarosz-Wilkolazka Contact person: Anna Jarosz-Wilkolazka, Email: [email protected]

Phone: (48 81) 537 50 44Deadline for application: Remarks: Educational and professional goals - student knows the main groups of secondary metabolites produced by microorganisms and plants; can indicate a correlation between structure of secondary metabolites such as alkaloids, terpenoids and antibiotics and their biological activities; is aware of chemical and biological diversities of natural environment.


5.Course title: Microbiology

Faculty/Department: Faculty of Biology and Biotechnology, Department of Genetics and MicrobiologyCourse code: MIRWMTeacher: prof. dr hab. Wanda Małek ([email protected]) (48) 81 537 59 76 Lecture/Laboratory: 30 hrs / 60 hrs ECTS credits: 8 Year of study: II (I0), winter semesterEducational and professional goals The purpose of microbiology course is to familiarize students with the major groups of prokaryotes, their cell structure, function, metabolism, and the role of microorganisms in the environment as well as biotechnology. The student learns the techniques used to identify, differentiate microorganisms as well as know the basic strategies and methods to combat pathogenic bacteria. Course description:the lectures:Microbiology beginning. The world of microorganisms. The rules of contemporary classification of microorganisms. Prokaryotic cell structure and functions. Endospores and other resting forms of bacteria. Microbial nutrition: requirements for carbon, nitrogen, iron, phosphorus, sulfur, oxygen, hydrogen. Nutritional types of microorganisms. Microbial growth: measurement of cell number and cell mass, the growth in closed and continuous culture systems. Bacterial biofilms. Metabolism: aerobic and anaerobic respirations, fermentations, chemosynthesis, photosynthesis. Control of microorganism growth by physical and chemical agents. Bacteriophages: structure, lytic and lysogenic cycles. Economic and environmental importance of bacteria.the classes:Comparison of different types of bacterial cell wall structure: Gram-negative, Gram-positive, acid-resistant (Gram and Ziehl-Neelsen staining methods). Cytology of bacterial cell: the staining methods of cell wall, genetic material, endospores, capsules. The microbiological techniques: isolation of pure cultures, identification of bacteria on the basis of morphological and physiological features. Measure of the bacterial growth. A types of microbiological culture media: preparation, characteristics. Control of microorganisms: sterilization and disinfection. The effect of environmental factors on bacterial growth: temperature, UV, osmotic pressure, pH, antibiotics. Interactions between organisms: mutualism, antagonism. Identification of microorganisms in dairy products. Bacteriophages: isolation, bacteriophage plaque-count assay, host range determination.Literature:“Biology of Microorganisms”- Michael T. Madigan, John M. Martinko, Jack Parker, Prentice Hall International, Inc; “Microbial Life”- Jerome J. Perry, James T. Staley, Stephan Lory, Sinauer Associates, Publisher Sunderland, MassachusettsAssesment method: oral or written exam Prerequisities: basic knowledge of EnglishPrimary target group: biologists, biotechnologists

6.Course title: Immunology


(+48) 81 537 59 43 Magdalena Mizerska-Dudka, Ph.D. ([email protected]) Lecture/Laboratory: 30 hrs/45 hrs ECTS credits: 6.5 Year of study: III (I0), summer semester

Educational and professional goals The course provides an extensive theoretical and technical study of mechanisms of the immune response in physiological and pathological conditions. Upon completion of the course, the student should be able to: explain mechanisms leading to immune response, name new trends in developing of diagnostic methods and immune therapy, define and perform the basic immunological test used in practice, estimate of immune therapy effectiveness in pathology, orientate for prohealth behavior.

Course description

Lecture contents: Development of central and peripheral immune system. Innate immune system: function, anatomical barriers, inflammation, complement system, phagocytosis. Adaptive immune system: function, structure and characteristic of antigens and antibodies, antigen-antibody reactions, antigen presentation, membrane receptors for antigen, lymphocyte activation, humoral and cellular immune response, the production of effectors, vaccines, immunodeficiency, hypersensivity. Immunological methods and applications. Laboratory exercise contents: Methods of study innate and adaptive immunological response. Central and peripheral immune system, isolation of cells from lymphatic organs. Phagocytosis determination. Proliferation and differentiation of lymphocytes. Rosette test. Major histocompatibility antigens. Blastic transformation of lymphocytes. Macrophage migration inhibition test. Detection of cells producing antibodies. Some techniques used in immunodiagnosis: agglutination, precypitation, ELISA, Latex tests and some methods used in preparation of monoclonal antibodies.Literature:

1. "Roitt's Essential Immunology" P.J.Delves, S.J.Martin, D.R.Burton, I.M.Roitt (2011)2. "Kuby Immunology" sixth ed. T.J.Kindt, B.A.Osborne, R.A.Goldsby



7.Course title: Ecology

Faculty/Department: Faculty of Biology and Biotechnology, Department of EcologyCourse code: EKOBCTeacher/Contact person: prof. dr hab. Bożenna Czarnecka (e-mail: [email protected]), tel. 48 81 537 59 30Number of hours: Lecture/Laboratory 30 hrs / 45 hrs ECTS credits: 6,5Course duration: summer semesterEducational and professional goals: The course is designed to allow all the students to broaden their knowledge about interactions among different groups of organisms and their environment.Course description:the lectures:The scope of the content covers the basics of general ecology, including the relationships among organisms and between organisms and the environment occurring at different levels of biological organization; ecological processes; matter exchange and energy balance in the biosphere. Students will also be introduced to current research problems of modern ecology and the nature of its relationship with many areas of mathematical, socio-economic, and natural sciences. the classes:The course provides theoretical and practical study of the following issues: types of growth forms among plants and animals, life-histories and adaptive strategies, population ecology, vegetation structure in natural and anthropogenic landscape, ecological processes at the level of plant community (succession, regression, fluctuation, regeneration, degeneration, seasonal dynamics), basic methodology of field ecological observations, designing and conducting simple observations and experiments in laboratory and natural habitats and interpretation of their results, survey of basic habitat characteristic, measurement of soil and water properties, diagnosis of the state of the environment on the basis of indicator species and plant communities, preparation and presentation of field research report. Literature:1. Begon M., Townsend C.R. Harper J.L. 2006. Ecology. From Individuals to Ecosystems. Blackwell

Publishing, Malden, USA. 2. Crawley M.J. 1997. Plant Ecology. Blackwell Science, Oxford, UK.Course type: lecture, laboratory with outdoor practiceForm of assessment Lecture: the written test exam composed of different forms of questions (true/false, multiple choice, short answer); Laboratory: written test and field research reportPrerequisities: Basic knowledge of English, basic knowledge of botany and zoologyPrimary target group: Biology, Biotechnology and Environmental Science Students

8. Course title: Plant Embryology

Faculty/Department: Faculty of Biology and Biotechnology, Department of Plant Anatomy and Cytology Course code: EMRJBTeacher/Contact person: Krystyna Winiarczyk ; [email protected] of contact hours: Lecture/Laboratory 30 hrs /45 hrsECTS credits: 6Course duration: I (IIº) year, winter semester Educational and professional goals:

To gain knowledge in the field of embryology, especially in structures and functions of organs involved in sexual reproduction of flowering plants.

To develop skills in preparation of microscopic slides and interpretation of tissue sections

Course description:the lectures:Heterogenic cycle and sex determination in plants. Bryophytes: sporangium and sporogenesis; archegonia, antheridia and gametes. Generative organs of ferns and horsetails. Heterospory in club mosses and water ferns. Gymnosperms: pollen sac development, microsporogenesis, pollen grain development, ovule development, macrosporogenesis, perisperm, archegonia, embryo. Angiosperms: anther development, microsporogenesis, pollen development, types of: ovules, macrosporogenesis and embryo sacs; pollination, progamic phase, fertilization, embryo, seed development, apomixes.

the classes:

Practical preparation of microscopic slides Methods of tissue section staining Use of different types of microscopic techniques

Literature:1 Lersten N.R. Flowering Plant Embryology. 2004 2 Batygina T.B. Embryology of Flowering Plants vol 1,2,3. 2009Course type: Lecture, Laboratory Form of assessment: Test (written examination), continuous assessment of laboratory Prerequisities: Completed course in: general and taxonomic botany; plant anatomyPrimary target group: student’s of general biology course

9. Course title: Animal histology and embryology

Faculty/Department: Faculty of Biology and Biotechnology, Department of Comparative Anatomy and AnthropologyCourse code: EHZAGTeacher/Contact person: Monika Hułas-Stasiak, [email protected] 81 5397 59 08 Joanna Jakubowicz-Gil, [email protected], 81 5397 59 08 Number of contact hours: Laboratory 90 hrsECTS credits: 6.5Course duration: III year (I°), summer semesterEducational and professional goals

To acquire an in –depth knowledge of human body structure at the microscopic level To know the relationship between the histological structure and function of

differentiated/specialized cell types, tissue types and organs of human To develop an understanding how organ integrity and functions are maintained by the

organization of cells and tissues To identify cells and tissues and describe their function To observe and study the microscopic anatomy of selected differentiated/specialized cell

types, tissue types and organs of human To develop a professional histological terminology To know some of the techniques that are used to investigate histology To gain experience in reading and evaluating scientific literature To become familiar with some of the clinical applications of histology in health and disease. To introduce students to developmental anatomy of the human and animal embryo. To emphasis anatomical change with some discussion of developmental mechanism and

physiology.

Course description:the classes:Histology is an introduction to the microscopic structure of cells, tissues and organs. The emphasis of the course is on the study of human body. This course provides the students with the opportunity to use the light microscope to study stained and mounted sections of mammalian tissues. The aim of this course is to allow the students to gain an understanding of the human body on a microscopic level and to develop an appreciation of intricate relationship among various organ systems. The focus of embryology is on the anatomy of vertebrate embryogenesis with specific emphasis on humans. Topics include fertilization, implantation, gastrulation, neurulation and organogenesis of a variety of structures.

Literature:1 Sadler TW. Medical Embryology. Lippincott Williams and Wilkins, 20062 Alan Stevens, James Lowe. Human Histology, 2010 Course type: lecture and practical laboratory Form of assessment written exam Prerequisities: basic knowledge of English Primary target group: biologists, biotechnologists



10. Course title: Functional Human Anatomy:

Faculty/Department: Faculty of Biology and Biotechnology, Department of Comparative Anatomy and AnthropologyCourse code: AFRAGTeacher/Contact person: Dobrowolski Piotr PhD ([email protected]), Tel.: +48 507 132 520Number of contact hours: Lecture/Laboratory 30 hrs / 30 hrsECTS credits: 3,5Course duration: Year of study I (I°) (winter semester) Educational and professional goals:The student can describe the human anatomy; gives and explains the functions of anatomical organs; is able to explain the relationship between structure and function of tissues, organs and anatomical systems. Has ability to lead microscopic observations and present the interpretation of images of histological sections; based on macro- and microscopic details recognizes the organs of the body; has knowledge of how to determine the root causes of disease and to recognize their symptoms.Course description:the lectures:Human body: parts of the body, axes, planes and metamerism of the body. Body tissues. Topography, structure and function of organ systems: skeletal, muscular, digestive, respiratory, cardiovascular, urinary, reproductive, nervous, endocrine, the organs of the senses, integumentary system. Intraspecific differentiation. Elements of anthropology.the classes:Microscopic observation of different tissues: epithelial, muscular, connective and nervous. Macro- and microscopic observations of the structure of organs creating all the anatomical systems of human body: skeletal, muscular, digestive, respiratory, cardiovascular, urinary, reproductive, nervous, endocrine, the organs of the senses and integumentary system. Elements of anthropometry.Literature:1 Drake, Vogl, Mitchell “Gray’s Anatomy for Students”;2 Abrahams, Boom, Spratt, Hutchings „Clinical Atlas of Human Anatomy”;3 Fenneis “Pocket Atlas of Human Anatomy”;4 Sobotta “Atlas of Human Anatomy”.Course type: lecture and laboratoryForm of assessment Continuous assessment in a form of written tests - several tests during the course and final written test.Prerequisities: Completed course in cell biologyPrimary target group: …………………………………………………………………………..

11. Course title: Developmental biology of plants and animals

Faculty/Department: Faculty of Biology and Biotechnology, Department of Plant Anatomy and Cytology, Department of Comparative Anatomy and AnthropologyCourse code: BROESTeacher/Contact person: dr hab. Ewa Szczuka Prof. Assoc. UMCS, (e-mail address) [email protected] tel. +48 81 5375061dr Dorota Tchórzewska, (e-mail address) [email protected] dr Marcin Domaciuk, (e-mail address) [email protected] Joanna Jakubowicz-Gil (e-mail address) [email protected] Mariusz Niedźwiedź (e-mail address) [email protected] of contact hours: Lecture/Laboratory 60 hrs / 90 hrs / 60 hrs of field classes ECTS credits: 16Course duration: I (Iº) year, winter and summer semesterEducational and professional goalsUnderstanding the importance of sexual reproduction for biodiversity and evolutionary variation of organisms; describing the stages of development of plants and animals - gametogenesis, fertilization, embryogenesis, organogenesis, their mechanisms and determining factors. Knowledge and skills in application of microscopic techniques and methods of field observation, independent planning research and analyzing results. Awareness of the need to update and integrate knowledge of botany and zoology.Course description:lectures:Sexual reproduction as a driving power of organisms’ variability and evolution. Life cycles of plants. Determination of differentiation and morphogenesis processes. Development of gametophytes and sporophytes; formation of plant tissues and organs. Sexual reproduction in particular taxa. Genetics of flower development; apomixis, somatic embryos, synthetic seeds. Mechanisms of animal development. Gametogenesis, recognition of gametes, fertilization. Prevention of polyspermy. Embryogenesis: formation of the germ layers in vertebrates, arising of organs. Cloning. Apoptosis. Stem cells. classes:Sexual reproduction as a driving power of organisms’ variability and evolution. Heterogenic cycle and sex determination in plants. Determination of differentiation and morphogenesis processes. Development of gametophytes and sporophytes; forming of plant tissues and organs. Sexual reproduction in particular taxons. Genetics of flower development, pollination, progamic phase, fertilization, embryo and seed development; apomixis, somatic embryos, synthetic seeds. Plant cell organelles. Mechanisms of animals development. Gametogenesis, recognition of gametes, fertilization. Prevention of polyspermy. Embryogenesis: formation of the germ layers in vertebrates, arising of organs. Anatomy and histology of the reproductive system. Embryology of amphibians, birds, mammals. Literature:1. Lersten N.R. Flowering Plant Embryology. Blackwell Publishing 20042. Raghavan V. Developmental Biology of Flowering Plants. Springer-Verlag 20003. Wang T.L. Cuming A. Embryogenesis the generation of a plant. Bios 20064. Gilbert S.F. Developmental Biology. Sinauer 20035. Sadler T.W. Medical Embryology. Lippincott Williams & Wilkins 20066. Bronner-Foster M. (Editor): Avian Embryology. Academic press 2011 Course type: lecture, laboratory, field classes. Form of assessment: written examPrerequisites: basic course of botany and zoology - completed course in general and taxonomic botany and zoologyPrimary target group: students of biotechnology and biology

12. Course title: Basic techniques of cell and tissue culture Faculty/Department: Faculty of Biology and Biotechnology/Department of Plant Anatomy and Cytology Course code: PHTRPTeacher/Contact person:

Mgr Kinga Lewtak, [email protected], +48 81 537 50 97Dr Ewa Dziadczyk, [email protected], +48 81 537 50 05Dr Marcin Domaciuk, [email protected], +48 (81) 537 50 05

Number of contact hours: Lecture/Laboratory: -/60 hrsECTS credits: 6Course duration: Year of study: III (Iº), winter or summer semester Educational and professional goals:The purpose of Basic techniques of cell and tissue culture course is to familiarize students with characteristics, types and uses of different plant tissue cultures. The student learns the techniques used to establish axenic plant tissue cultures and ways of working in sterile conditions.Course description: Plant cell and tissue culture laboratory – basic equipment and organization of work. The main techniques of sterilization and preparation of plant material. Components of culture media and preparation procedures. Hormonal control of cell growth and development. Organogenesis induction and plant regeneration from cultured explants on solid media. Initiation and establishment of callus culture from different types of explants. Meristem culture (isolation of shoot apical meristems). In vitro clonal propagation of crop plants (method of micropropagation from axillary buds). Establishment of cell suspension culture and its application in biotechnology. Literature: 1. Evans D.E., Coleman J.O.D., Kearns A., Plant Cell Culture. BIOS Scientific Publishers, 20032. Smith R.H., Plant Tissue Culture. Techniques and Experiments. Academic Press, Second Edition, 2000 Course type: LaboratoryForm of assessment: Written test questionsPrerequisities: Plant physiology, biochemistry, genetics, basic knowledge of EnglishPrimary target group: biologists, biotechnologists

13.Course title: Methods of in vitro tissue culture

Faculty/Department: Faculty of Biology and Biotechnology/Department of Plant Anatomy and Cytology Course code: MKTBPTeacher/Contact person:

Mgr Kinga Lewtak, [email protected], +48 81 537 50 97Dr Ewa Dziadczyk, [email protected], +48 81 537 50 05Dgr Marcin Domaciuk, [email protected], +48 (81) 537 50 05

Number of contact hours: Lecture/Laboratory: -/30 hrsCourse duration: Year of study: II (IIº), winter or summer semester ECTS credits: 3,5Educational and professional goals:The purpose of Methods of in vitro tissue culture course is to familiarize students with characteristics, types and uses of different plant tissue cultures. The student learns the techniques used to establish axenic plant tissue cultures and ways of working in sterile conditions.Course description: Basic equipment and organization of work in plant cell and tissue culture laboratory. Components of culture media and preparation procedures. Stages of preparation of sterile culture media; proliferation of cell mass; hormonal development orientation (direct and indirect regeneration). Hormonal control of cell growth and development. Organogenesis induction and plant regeneration from cultured explants on solid media. Initiation and establishment of callus culture from different types of explants. Meristem culture (isolation of shoot apical meristems). In vitro clonal propagation of crop plants (method of micropropagation from axillary buds). Establishment of cell suspension culture and its application in biotechnology. Literature: 1. Evans D.E., Coleman J.O.D., Kearns A., Plant Cell Culture. BIOS Scientific Publishers, 20032. Smith R.H., Plant Tissue Culture. Techniques and Experiments. Academic Press, Second Edition, 20003. Pollard J.W., Walker J.M. Plant Cell and Tissue Culture, Human Press 1990 Course type: LaboratoryForm of assessment: Written and oral assessmentPrerequisities: Plant physiology, biochemistry, genetics, basic knowledge of EnglishPrimary target group: biologists, biotechnologists

14. Course title: Invertebrate immunology

Faculty/Department: Faculty of Biology and Biotechnology, Department of ImmunobiologyCourse code: IMBMCTeacher/Contact person: dr hab. Małgorzata Cytryńska ([email protected]), dr hab. Iwona Wojda ([email protected]), dr Agnieszka Zdybicka-Barabas ([email protected]), mgr Sylwia Stączek ([email protected])Contact person: dr hab. Małgorzata Cytryńska ([email protected]), Tel: 81 537 50 50.Number of contact hours: Lecture/Laboratory 15 hrs/25 hrsECTS credits: 3.5 Course duration: year of study - I (IIo); summer semesterEducational and professional goalsKnowledge of the essential mechanisms of invertebrate immunity. Understanding the differences between invertebrate and vertebrate immune response. The student: can explain the complex mechanisms leading to activation of the immune response in invertebrates and the mechanisms of overcoming the insect immunity by entomopathogenic organisms; can use an integrated knowledge of the various fields of biology (biochemistry, microbiology, immunology); knows and applies the techniques and methods used in research on invertebrate immunity; is able to use basic laboratory equipment; properly interprets the empirical data. Course description:the lectures:Essential features of invertebrate immunity on the example of insect innate immunity. Recognition of non-self (pathogen associated molecular patterns, pattern recognition receptors). Mechanisms of invertebrate immunity: anatomical and physiological barriers; cellular response (types of hemocytes, phagocytosis, nodulation, encapsulation); humoral response (hemolymph coagulation, phenoloxidase system, defense peptides and proteins). Regulation of gene expression of defense peptides in Drosophila. The role of proteins containing immunoglobulin domains in invertebrate immunity (hemolin, Dscam, FREPs). Entomopathogenic organisms.the classes:Galleria mellonella (Lepidoptera) as a model organism (isolation of fat body; microscopic observation of hemocytes). Analysis of phenoloxidase activity in hemolymph of naive and immune-challenged insects. Detection and analysis of antimicrobial activity (lysozyme, defense peptides) in G. mellonella hemolymph. Comparison of the susceptibility of different bacteria to G. mellonella defense peptides. The role of proteases of entomopathogenic bacteria in overcoming the insect immune response.Literature:

1. Insect Molecular Biology and Biochemistry, Lawrence I. Gilbert (Ed.), Elsevier, Academic Press 2012.

2. Recommended review papers of the current scientific literature.Course type: lecture and practical laboratory classesForm of assessment: written exam (lecture); continuous evaluation of the laboratory classesPrerequisities: knowledge in biochemistry and microbiologyPrimary target group: students of biology, biotechnology

15. Course title: Mycology

Faculty/Department: Faculty of Biology and Biotechnology, Department of Botany and MycologyCourse code: MIKWMTeacher: prof. dr hab. Wiesław Mułenko (e-mail: [email protected]); dr Urszula Świderska-Burek (e-mail: [email protected])Contact person: dr Urszula Świderska-Burek ([email protected]), tel. +48 81 5375092Number of contact hours: 45 (lecture 15 hrs / laboratory 30 hrs)ECTS credits: 3Course duration: I(Io) year, winter or summer semesterEducational and professional goals:Knowledge of the basic morphological and anatomical features of the fungal structure, distinguishing them from other groups of organisms, and modes of reproduction and propagation in the environment (specialist terminology). Knowledge of the role and importance of fungi in the natural environment; protection of fungi as the natural components of the biosphere; mutual relationships between fungi and other organisms – parasitism, commensalism, mutualism; mycorrhizal fungi, endophytes. Ability to identify and distinguish the main edible and poisonous species and identification of symptoms of diseases.

Course description:the lectures:Structure of fungi and fungus-like organisms. Specific components of fungal cells. Vegetative and generative fungal structures. Morphology and anatomy of fungal fruiting bodies. Types of asexual and sexual reproduction, sporogenesis, spore propagation. Trophic forms. Fungal metabolites and their application. The role and importance of fungi in the ecosystem. Modern criteria for classification of fungi, an overview of major taxa (The Code of Nomenclature).the classes:Cell and thallus structure of true fungi and fungi-like organisms. Characteristic of the most important vegetative and generative fungal structure. Characteristics of selected substrate types of resettlement substrates colonized by fungi; the characteristics of the eucarpic fungi. Characteristic of selected representatives of Myxomycota, Oomycota, Zygomycota, Ascomycota, Basidiomycota, anamorphic fungi, and Lichenes. Structure, development, reproduction, spreading of parasites, saprotrophs, symbionts and endophytes.

Literature:1. Alexopoulos C.J. 1952. Introductory Mycology. John Wiley & Sons, New York, pp. 482.2. Deacon J. 2006. Fungal biology. 4th edition, Blackwell Publishing, pp. 371.3 Ingold C.T. 1961. The Biology of Fungi. Hutchinson Educational, pp. 124.4. Moore D., Robson G.D., Trinci A.P.J. 2011. 21st Century Guidebook to Fungi. Cambridge University Press,

New York, pp. 627.Course type: lecture and practical laboratoryForm of assessment: written test questionsPrerequisities: basic knowledge of EnglishPrimary target group: students of biology

16. Course title: General and Systematic Botany

Faculty/Department: Faculty of Biology and Biotechnology, Department of Botany and MycologyCourse code: BOSWMTeacher: prof. dr hab. Wiesław Mułenko (e-mail: [email protected]), tel. +48 81 5375938; mgr Magdalena Pluta ([email protected]); dr Monika Kozłowska ([email protected]); dr Aneta Ptaszyńska ([email protected]); dr Urszula Świderska-Burek ([email protected]); dr Agata Wołczańska ([email protected]); dr Robert Zubel ([email protected])Contact person: dr Urszula Świderska-Burek (e-mail: [email protected]), tel. +48 81 5375092Number of contact hours: 105 hrs (lecture 30 hrs + laboratory 60 hrs + field classes 30 hrs)ECTS credits: 8.5Course duration: I(Io) year, summer or winter semesterEducational and professional goals:Knowledge of the basic morphological and anatomical features of the plant structure, their classification and modifications; knowledge of the role and importance of plants in the natural environment as the key components of the biosphere; plant protection; adaptation to environmental conditions; the role of plants in human life; plant use.Skills to prepare microscope slides of various plant structures. Ability to identify the most important plant species. Efficient use of appropriate equipment and keys for identification of organisms.Course description:the lectures:Plant cell structure. Forms of organization of plant organism. The structure and classification of tissues. Morphology, anatomy, and modifications of vascular plant organs (root, stem, leaves). Flowers and inflorescences – the structure, classification, modes of pollination. Fruits and seeds – development, structure, classification, and propagation modes. Modes of plant propagation, alternation of generations. An overview of major plant systems; the rules of botanical nomenclature, the code of nomenclature. The main hypothesis of the origin and evolution of axial plants and thallophytes. A systematic overview of the main strategies in plant development. An overview of systematic groups with particular emphasis on seed plants as well as protected and crop species.the classes:The basic features of the structure of plant cell; examples of various forms of organization of the body of lower plants, the concept of the thallus, types of thalli (examples). Plant tissues and their functional systems. Construction of the most important plant organs and their modifications (root, stem, flower, fruit). Systematics: prokaryotic and eukaryotic algae; characteristic features of the structure of hornworts, liverworts, mosses, and pteridophytes; morphological and anatomical structure of selected organs and identification of gymnosperms and angiosperms (monocotyledons and dicotyledons).the field classes:General characteristics of the Roztocze National Park and Kazimierz Landscape Park. Characteristic forest communities (coniferous forest, mixed forests, Carpathian beechwood, oak-hornbeam forest) and xerothermic (grasslands, scrub). Knowledge of the flora of the examined areas, species composition, leading species, protected plants. Problems of a comprehensive protection of nature.Literature:1. Eames A.J., MacDaniels L.H. 1947. An introduction to plant anatomy. Mc Graw - Hill Book Company, pp.

427.2. Esau K. 1977. Anatomy of seed plants. John Wiley & Sons, New York, pp. 550.Course type: lecture, practical laboratory and field classesForm of assessment: written examination (lecture) and test questions (laboratory); herbarium (after field exercises)Prerequisities: basic knowledge of EnglishPrimary target group: students of biology

17. Course title: Mechanisms of bacterial infections

Faculty/Department: Faculty of Biology and Biotechnology, Department of Genetics and MicrobiologyCourse code: Teacher/Contact person: dr hab. Urbanik-Sypniewska Teresa, prof. UMCS (email address), [email protected] tel. (81) 537-50-33

Number of contact hours: Lecture/Laboratory: 15 hrs / –

ECTS credits: 1

Course duration Year of study: II (II°), summer semester

Educational and professional goals:

The course provides a basic mechanisms of bacterial pathogenicity, the molecular strategies used by bacteria to interact with the host. The student can convert the acquired theoretical background knowledge into actual practice.

Course description: the lectures:Host-pathogen interactions in bacterial infections. Pathogenicity - infection - disease. The normal bacterial flora of humans - the opportunistic infections. The exogenous infections. Bacterial virulence factors: adhesins, invasins, impedins, aggressins, modulins. Mechanisms involved in bacterial adhesion. Bacterial invasion. Bacterial toxins: endo- and exotoxins. Evasion of host defence mechanisms: evasion of immune defences at mucosal level, evasion of innate immune mechanisms, evasion of acquired immunity. Regulation of virulence factors. Intracellular parasites. The problem of massive overuse of antibiotics. Identification of bacterial virulence genes. Development of new antibacterials.

Literature:1. Microbiology A System Approach 2009 sec. ed. M.K. Cowan, K.P. Talaro2. . Medical Biotechnology 2009 J. Pongracz, M. Keen3. Molecular Detection of Bacterial Pathogens 2011, Ed. D. Liu, CRC Press

Course type:

optional

Required background:

knowledge of elements of microbiology, immunology

Form of assessment:

short presentation

18. Course title: Medical microbiology Faculty/Department: Faculty of Biology and Biotechnology, Department of Genetics and MicrobiologyCourse code: Teacher/Contact person: dr hab. Urbanik-Sypniewska Teresa, prof. UMCS (email address), [email protected] tel. (81) 537-50-33, dr Wdowiak-Wróbel Sylwia, (email address), [email protected] of contact hours: Lecture/Laboratory 30 hrs / 60 hrsECTS credits: 8Course duration Year of study: I (II°), summer semester Educational and professional goals The course provides a basic theoretical and technical study of the structure, molecular biology, pathogenesis, epidemiology, and laboratory identification of the various bacteria that cause human diseases. The student can convert the acquired theoretical background knowledge into actual practice in the course of experiments. University and government laboratories, research institutes, the pharmaceutical and biotechnology industries, and diagnostic laboratories are employers looking for qualified microbiologists. Course description:

the lectures: Introduction to Medical Microbiology: Normal flora (human microbiome), Gram-positive cocci: Staphylococcus, Streptococcus, Enterococcus, Gram-positive spore-formers: Bacillus, Clostridium. Anaerobic infections: Bacteroides, Porphyromonas, Prevotella, Fusobacterium. Gram-negative bacilli: Enterobacteriaceae; Yersinia, Salmonella, Shigella, Escherichia, coliforms, Klebsiella, Proteus, Gram-negative bacilli oxidase positive Pseudomonas, Burkholderia, Gram-negative curved bacilli: Vibrio, Campylobacter, Helicobacter, Gram-negative coccobacilli: Brucella, Bordetella. Fastidious Gram-negative bacteria: Neisseria, Haemophilus, HACEK group infections, Legionella, Coxiella. Animal-associated bacteria: Erysipelotrix, Francisella, Pasteurella, Mannheimia. Spirochetes: Treponema, Leptospira, Borrelia, Gram-positive bacilli: Listeria, Corynebacteria, Mycobacteria. Obligate intracellular bacteria: Rickettsia, Chlamydia. Cell wall-less bacteria: Mycoplasma, Ureaplasma. Antimicrobial Chemotherapy.

the classes:Isolation and culturing of bacteria. Microscopic examination of bacterial morphology by different staining methods. Examination and identification of selected groups of pathogenic bacteria by using laboratory detection methods: molecular, morphological, immunological and cultural. Examination of factors affecting disease spread. Biochemical test for identification of bacteria /API-testing/. Evaluation of the efficacy of antimicrobial agents; determination of MIC and MBC. PCR methods for virulence factors detection.

Literature:1. Harrison’s Infectious Diseases. Dennis L. Kasper, Anthony S. Fauci

2010. The McGraw-Hill Companies, Inc.: New York. ISBN: (Paperback) 978-0071702935.2. Microbiology: Diversity, Disease, and the Environment. by Abigail A. Salyers and, Dixie D.

Whitt. Fitzgerald Science Press3. Microbiology with diseases by body system. 3-rd Ed. R.W. Bauman, PEARSON, + (2012)

Course type:an extensive course, lecture and practical laboratory classesForm of assessment: The written test exam composed of test questions e.g. True/False, Matching, Multiple Choice and Short AnswerPrerequisities: biology coursePrimary target group:

http://www.amazon.com/s/ref=ntt_athr_dp_sr_2?ie=UTF8&field-author=Dixie+D.+Whitt&search-alias=books&text=Dixie+D.+Whitt&sort=relevancerank

http://www.amazon.com/s/ref=ntt_athr_dp_sr_2?ie=UTF8&field-author=Dixie+D.+Whitt&search-alias=books&text=Dixie+D.+Whitt&sort=relevancerank

http://www.amazon.com/Abigail-A.-Salyers/e/B001K6Q7L4/ref=ntt_athr_dp_pel_1

http://www.google.com/[email protected]

19. Course title: Microscopic techniques Faculty/Department: Faculty of Biology and Biotechnology, Department of Comparative Anatomy and Anthropology Course title: Microscopic techniquesCourse code: TEMBPTeacher/Cont act person: : Pawlikowska-Pawlęga Bożena, PhD ( [email protected] ), 537-59-28; 537-59-16; Pawelec Jarek, M.S. Wydrych Jerzy, M.S.Number of contact hours: Laboratory 30hrsECTS credits: 3Course duration: II (II o), summer semesterEducational and professional goals

Student characterizes selected microscopic techniques and explains their principles Student identifies particular cell organelles on microscopic slides Student uses techniques of biological preparation in the range of microscopic techniques Student selects appropriate microscopic techniques dependently on the sort of observation and

experiment Students knows safe and ergonomic working in the laboratory Student is aware of practical application of microscopic techniques and the importance of microscopic

research in biological and medical knowledgeCourse description:the classes

The exercises enable to earn theoretical and practical knowledge from different kind of light microscopy e.g. fluorescence, dark-field as well as confocal and electron-transmission and scanning. Main topics include: construction of electron and confocal microscope, construction and operation on light microscope-light and dark-field, fluorescent microscope, preparation of specimens for electron microscopy: trimming, cutting, contrasting of biological samples, grids observation under electron microscope.

Literature:1. Hayat M.A. 2000, Principles and Techniques of Electron Microscopy. Biological applications,

Cambridge University Press.2. Glauert A.M.,1974, Practical methods in Electron Microscopy, North-Holland/American Elsevier.3. J. Litwin, M. Gajda, WUJ 2011, Podstawy Technik Mikroskopowych.4. B. Wróbel, K. Zienkiewicz, D. Smoliński, J. Niedojadało, M. Świdziński, WUMK 2005, Podstawy

Mikroskopii Elektronowej.

Course type: lecture and practical laboratoryForm of assessment Continuous assessment in a form of tests- two tests during the course on which final mark is based Prerequisities The passed courses from cell biology, chemistry and biochemistry; basic knowledge of EnglishPrimary target group: students of biology and biotechnology


20. Course title: Molecular mechanisms of adaptation Faculty/Department: Faculty of Biology and Biotechnology, Department of ImmunobiologyCourse code: MMATJTeacher/Contact person: prof. dr hab. Teresa Jakubowicz ([email protected]), (48) 81537 5089, dr hab. Małgorzata Cytryńska ([email protected]), dr hab. Iwona Wojda ([email protected]), mgr Sylwia Staczek ([email protected])Number of contact hours: Lecture/Laboratory 30hrs /45hrs ECTS credits: 6,5Course duration: I (II0 ) year, summer semesterEducational and professional goals:The aim of the course is to give students comprehensive view of molecular mechanisms of pro- and eukaryotic organisms adaptation Course description:the lectures:Gene expression in humans and animals regulated by changes in environmental conditions. Gene and genome rearrangements. Adaptive genes. Methods used for organisms changeability determination. Adaptive character of immune responsethe classes: Influence of growth conditions on microorganisms extracellular proteases profiles. Analysis of lysozyme protein level and activity in Galleria mellonella after infection by saprophytic and pathogenic microorganisms. Comprehensive proteomic analysis of G. mellonella tissues after exposition to different stress conditions.Literature:

1. Fundamental Molecular Biology by Lizabeth A. Allison2. Selected positions of current scientific literature

Form of assessment oral or written examPrerequisities:completed course of biochemistry………………………………………………………..Primary target group:biology/biotechnology students……………………………………………………..




21. Course title: Molecular genetics

Faculty/Department:

Faculty of Biology and Biotechnology, Department of Immunobiology

Course code: GEMTJ

Teacher/Contact person: prof. dr hab. Teresa Jakubowicz ([email protected]), (48)

815375089

Number of contact hours: Lecture 30hrs

ECTS credits: 2

Course duration: II (II0 ) year, winter semester

Educational and professional goals:

The aim of the course is to introduce and discuss current topics related to molecular genetics of

eukaryotic organisms.

Course description:

the lectures:

Comparative genomics. Mechanisms of pre-RNA processing in eukaryotic cells. Types of splicing,

editing, catalytic RNA and DNA. Non-coding RNAs - biogenesis, physiological and pathological

role. RNA interference in vivo and therapeutic potential of RNAi. Epigenetic inheritance, epigenetic

mechanisms in human diseases. Genetic control of differentiation and development. Homeotic genes.

Cancer genetics. Chromatin regulation at mammalian telomers.

Literature:

1.Genomes by Brown T.A.

2.Genes by Lewin B.

3.Selected positions of current scientific literature

Form of assessment:

Oral or written exam

Prerequisites:

Completed course of biochemistry

Primary target group:

biology/biotechnology students


22. Course title: Bioinformatics – analysis of DNA and protein structure

Faculty/Department: Faculty of Biology and Biotechnology, Department of Molecular BiologyCourse code: BIKMGTeacher/Contact person: dr Michał Kalita, dr Przemysław Grela ([email protected]), tel (81)5375954Number of contact hours: /Laboratory / 30 hrs ECTS credits: 3Course duration: 30 hours, year I (IIo), summer semester

Educational and professional goals:The course will familiarize students with the tools and principles of DNA/protein structural bioinformatics. This course provides students with an overview of protein bioinformatics including computational and experimental approaches. It will introduce DNA, amino acid and protein physical properties as well as the alignment and evolution of DNA/protein sequences.

Course description:the classes:Protein structure and methods of structure determination will be presented as well as the use of protein databases and software for visualizing proteins. Methods for secondary and tertiary protein structure prediction will be discussed as well as methods for modeling small/molecule-protein interactions and protein-protein interactions. Finally, students will be introduced to experimental and computational aspects of mapping protein interaction networks. Computational methods for study of biological DNA sequence data in comparative biology and evolution. Analysis of genome DNA content and organization. Techniques for searching sequence databases, pairwise and multiple sequence alignment, phylogenetic methods, and methods for pattern recognition and functional inference from sequence data.

Literature:

1. Baxevanis, A.D., Ouellette, B.F.F. Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins 2. Higgs P.G., Attwood T.K. Paul G. Higgs and Teresa K. Attwood. (2005) Blackwell, Malden MA., Bioinformatics and Molecular Evolution

Course type: intermediateForm of assessment : continuous assessment of labsPrerequisities: basic knowledge of EnglishPrimary target group: biotechnologists

23. Course title: BioinformaticsFaculty/Department: Faculty of Biology and Biotechnology, Department of Molecular BiologyCourse code: BIFMGTeacher/Contact person: dr Michał Kalita, dr Przemysław Grela ([email protected]), tel (81)5375954Number of contact hours: Laboratory 10 hrs ECTS credits: 1Course duration: 10 hours, year II (IIo), winter and summer semester

Educational and professional goals:The course will familiarize students with the bioinformatic tools for searching DNA/ protein sequence databases and principles of DNA/protein structural bioinformatics. This course provides students with an overview of protein bioinformatics including computational and experimental approaches. It will introduce DNA, amino acid and protein physical properties as well as the alignment and evolution of DNA/protein sequences.

Course description:the classes:Introduction to usage of DNA/protein databases. Techniques for searching DNA/protein sequence databases. Pairwise and multiple sequence alignment, phylogenetic methods, constructing of phylogenetic trees, methods for pattern recognition and functional inference from sequence data. Analysis of genome content and organization. Computational methods for study of biological sequence data in comparative biology and evolution. Basics of protein structure and methods of structure determination will be presented as well as the software for visualizing 3D structures of proteins. Methods for secondary and tertiary protein structure prediction will be discussed as well as methods for modeling small/molecule-protein interactions and protein-protein interactions. Finally, students will be introduced to experimental and computational aspects of mapping protein interaction networks.

Literature:


Course type: beginnerForm of assessment : continuous assessment of labsPrerequisities: basic knowledge of EnglishPrimary target group: biologists, biotechnologists

24. Course title: Molecular Biology - an extensive course

Faculty/Department: Faculty of Biology and Biotechnology, Department of Molecular BiologyCourse code: BMRMTTeacher/Contact person: dr hab. Marek Tchórzewski ([email protected]), Tel. 815375956Number of contact hours: Lecture/Laboratory 45 hrs / 75 hrs ECTS credits: 10,5 Course duration: Io / year III, winter semesterEducational and professional goals The course will familiarize students with the essential concepts of molecular biology. The student learns the techniques used to analyse DNA, RNA and proteins.

Course description:the lectures:

The beginnings of molecular biology, the structure and role of DNA, RNA and proteins, transcription in Eukaryotes, RNA processing and posttranscriptional gene regulation, the mechanism of translation, signal transduction, apoptosis, molecular biology of cancer.

the classes:

Isolation of genome DNA and RNA from yeast cells, PCR, DNA and RNA agarose electrophoresis,analysis of chromatin composition in mammalian cells, detection of serine proteases inhibitors, protein electrophoresis in polyacrylamide gels, methods of protein staining after SDS/PAGE, transfection of mammalian cells, protein localization in cell by confocal microscopy.

1. J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin’s Genes XI2. L. A. Allison, Fundamental molecular biology3. T.A. Brown, Genomes 3

Course type: intermediateForm of assessment written exam, continuous assessment of labsPrerequisities: completed course in biochemistry Primary target group: biotechnologists

25. Course title: The basis of bioinformatics

Faculty/Department: Faculty of Biology and Biotechnology, Department of Molecular BiologyCourse code: PBIMGTeacher/Contact person: dr Michał Kalita, dr Przemysław Grela ([email protected]), tel (81)5375954Number of contact hours: Laboratory 20 hrs ECTS credits: 2Course duration: 20 hours, year III (Io), summer semester

Educational and professional goals:The course will familiarize students with the bioinformatic tools for searching DNA/ protein sequence databases and principles of DNA/protein structural bioinformatics. This course provides students with an overview of protein bioinformatics including computational and experimental approaches. It will introduce DNA, amino acid and protein physical properties as well as the alignment and evolution of DNA/protein sequences.

Course description:the classes:Introduction to usage of DNA/protein databases. Techniques for searching DNA/protein sequence databases. Pairwise and multiple sequence alignment, phylogenetic methods, constructing of phylogenetic trees, methods for pattern recognition and functional inference from sequence data. Analysis of genome content and organization. Computational methods for study of biological sequence data in comparative biology and evolution. Basics of protein structure and methods of structure determination will be presented as well as the software for visualizing 3D structures of proteins. Methods for secondary and tertiary protein structure prediction will be discussed as well as methods for modeling small/molecule-protein interactions and protein-protein interactions. Finally, students will be introduced to experimental and computational aspects of mapping protein interaction networks.

Literature:


Course type: beginerForm of assessment : continuous assessment of labsPrerequisities: ……………………………………………………………………………………..Primary target group: biotechnologists

26. Course title: Laboratory techniques

Faculty/Department: Faculty of Biology and Biotechnology, Department of Molecular Biology, Department of Genetics and MicrobiologyCourse code: TELCGTeacher/Contact person: Dr. Iwona Komaniecka ([email protected]), Dr. Anna Turska-Szewczuk ([email protected]); Dr. Leszek Wawiórka ([email protected]) Tel. 815375981; 815375954Number of contact hours: Laboratory 60 hrs ECTS credits: 6,5Course duration: II/III (Io) year, winter semesterEducational and professional goals Background and theory to understand the methods and techniques used in modern biology laboratories with a focus on structural biology, biochemistry, cell biology and microbiology disciplines.Course description:the classes: A study of laboratory operations, management, equipment, instrumentation, quality control techniques and safety procedures. Laboratory practice in using spectrophotometers, refractometers, polarimeters and pH meters, mixing buffers, performing measurements, preparing solutions, performing reparatory techniques to specifications; and practice laboratory safety. The course will also cover broad gas and liquid column chromatography techniques as well as nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry and the analysis of high-throughput mass spectrometry data. A survey of mass spectrometry ionization techniques and instrument types will be followed by an overview of data analysis techniques for protein identification, de novo protein sequencing, and the analysis of post-translational modifications. Finally, students will be introduced to novel protein expression and purification systems.

1. J. Sambrook, D. W. Russell, Molecular cloning-a laboratory manual, third edition.2. R.M. Silverstein, F.X. Webster, D.J. Kiemle, Spectrometric Identification of Organic

Compounds. Seventh edition (and further). John Wiley Sons, Inc.3. Analytical Chemistry – manual for students4. M.F. Chaplin and J.F. Kennedy, Carbohydrate Analysis. A Practical Approach. Second

edition.5. T. D.W. Claridge, High-Resolution NMR Techniques in Organic Chemistry. Pergamon,

1999.

Course type: intermediateForm of assessment written test questions, continuous assessment of labsPrerequisities: completed course in biochemistry, genetics, analytical and organic chemistryPrimary target group: biologists, biotechnologists

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