pensum$ cell$metabolism$ - ntnufolk.ntnu.no/jonathrg/fag/tbt4170/pensumslides/1 metabolisme.pdf ·...
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Cell metabolism
• Metabolism: – is the set of life sustaining chemical transforma4ons within cells.
– is usually divided into two categories: • Catabolism: Breaks down organic maFer and harvests energy by way of cellular respira4ons
• Anabolism: Uses energy to construct components of cells such as proteins and nucleic acids.
pensum
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Van Holde, Biochemistry
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Metabolic Diversity (1)
• The diversity in microbial cells is the product of almost 4 billion years of evolution
• Microorganisms differ in size, shape, motility, physiology, pathogenicity, etc.
• Microorganisms have exploited every conceivable means of obtaining energy from the environment
© 2012 Pearson Education, Inc.
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Figure 2.18
Organic chemicals
Inorganic chemicals
Chemotrophy Phototrophy
Chemicals Light
Energy Sources
(glucose, acetate, etc.) (H2, H2S, Fe2+, NH4+, etc.)
(glucose + O2 CO2 + H2O) (H2 + O2 H2O) (light)
Chemoorganotrophs Chemolithotrophs Phototrophs
© 2012 Pearson Education, Inc.
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Metabolic Diversity (2) • Chemoorganotrophs
– Obtain their energy from the oxidation of organic molecules (Figure 2.18)
– Aerobes use oxygen to obtain energy – Anaerobes obtain energy in the absence of oxygen
• Chemolithotrophs – Obtain their energy from the oxidation of inorganic
molecules (Figure 2.18) – Process found only in prokaryotes
• Phototrophs – Contain pigments that allow them to use light as an
energy source (Figure 2.18) – Oxygenic photosynthesis produces oxygen – Anoxygenic photosynthesis does not produce oxygen
© 2012 Pearson Education, Inc.
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Metabolic Diversity (3) • All cells require carbon as a major nutrient
– Autotrophs • Use carbon dioxide as their carbon source • Sometimes referred to as primary producers
– Heterotrophs • Require one or more organic molecules for their carbon
source • Feed directly on autotrophs or live off products produced
by autotrophs • Organisms that inhabit extreme environments are
called extremophiles. Habitats include boiling hot springs, glaciers, extremely salty bodies of water, and high-pH environments
© 2012 Pearson Education, Inc.
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Lehninger, Biochemistry
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Van Holde, Biochemistry
Pensum unntaF kjemisk struktur for adenosin
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Energy / Energy storage • Prime energy currency in all cells: ATP: Adenosin Triphosphate:
– ATP -> ADP + Pi ; energy released: 32 kJ/mol
• Energy (electron) carrier: – NAD: Nico4namide adenine dinucleo4de – FAD: Flavin adenine dinucleo4de
• Energy produced from catabolism and from ion channels (crossing of membranes)
• Long-term energy storage involves insoluble polymers that can be oxidized to
generate ATP – Examples in prokaryotes
• Glycogen • Poly-β-hydroxybutyrate and other
polyhydroxyalkanoates • Elemental sulfur
– Examples in eukaryotes • Starch • Lipids (simple fats)
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The molecular building blocks of the cells
Macromolecules/polymers: • Carbohydrates
– Monosaccharides (glucose) major building block – Func4on: Cell wall component and storage of energy
• Nucleic acids – Nucleo4des building blocks – Func4on: Informa4on storage, transmission and expression
• Proteins – Amino acids building blocks – Func4on: Enzymes, Structure, transport
• Lipids (phospholipids) – Gycerol and faFy acids (and phosphate) structural components – Func4on: Membranes (plasma membrane and intracellular compartments)
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Glucose metabolism -‐ overview
Starch Glycogen
Glucose (6C)
Pyruvat (3C)
Acetyl-‐CoA (2C)
CO2
Ethanol (2C) + CO2 Lac4c acid (3C) Ace4c acid (C2) Formic acid (C1)
2 CO2
e -‐
ATP
e -‐
NAD+ + H+ + 2e-‐ NADH
ATP ADP O2 H2O
Fermenta4on (without O2)
Respira4on (with O2)
TCA = sitronsyresyklusen
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Yeast w/wo oxygen (Renneberg fig 1.20 and text)
• Respira4on (w O2) – wanted for the yeast – C6H12O6 + 6O2 -‐> energy + 6CO2 + 6H2O – produc4on of cell mass (cell growth/prolifera4on) – Energy produced: Up to 38 ATP per glucose molecule – Produc4on of energy via glycolysis and TCA
• Fermenta4on (wo O2) – not ideal for the yeast – C6H12O6 -‐> 2C2H5O2 + 2CO2 – Far less produc4on of cell mass than under respira4on (5%) – Energy produced: 1-‐4 ATP per glucose molecule – Because of need of energy, produced by glycolysis of glucose to pyruvate, a higher throughput of mass is needed and hence a larger consump4on of glucose and produc4on of by-‐products (e.g. ethanol)(Pasteur effect)
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Gjær m/u oksygen • M/u oksygen bestemmer genuFrykk
– Når O2 blir lav endres genuFrykk slik at andre enzymer uFrykkes i anaerob metabolisme versus aerob metabolisme
• Anaerob fermentering, andre mikroorganismer – Melkesyre (Lactobacillus) (pluss flere eksempler): Biprodukt under produksjon av ATP ved anaerobe be4ngelser
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Cell metabolism -‐ overview • Metabolism defini4on
– What is cell metabolism? • Metabolic diversity
– How do different microorganism obtain energy? Carbon? • Energy carriers
– What are important long term and short term energy carriers in the cells? • Cellular macromolecules
– What are the major cellular macromolecules and what are their building blocks • Transforma4on of glucose to relevant products
– Give three relevant biotechnological products from glucose metabolism (names and relevance)
• No oxygen present: Produc4on of ethanol or lac4c acid – What are the products of respira4on and fermenta4on in yeast
• Major metabolic pathways – Glucose can eventually be metabolized to polysaccharides, DNA/RNA, proteins,
and phospholipids. What are the star4ng metabolite(s) seen from the glycolysis for the various macromolecules? (this is beyond “pensum”)
• Ethics? – Are there any ethical concerns related to what we have learned in Chapter 1 and
about cells and cell metabolism?