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Liquid Handling PCR & Molecular Biology Certified High-Quality Products for PCR, Molecular Biology and Research sterile · non-pyrogenic · DNA-free RNase-free · ATP-free

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Page 1: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

Liquid Handling

PCR & Molecular BiologyCertified High-Quality Products for PCR, Molecular Biology and Research

sterile · non-pyrogenic · DNA-freeRNase-free · ATP-free

Page 2: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

SARSTEDT InternationalYour Partner in medicine and science worldwide

Page 3: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

Table of Contents

Sarstedt quality symbols A warranty of reliability and reproducibility p. 4

Biosphere® Purity Certificate p. 4

PCR Performance Tested Purity Certificate p. 4

Pipette tips Crucial for precise dosing p. 5 - 19

Aerosol filters – Protection against carry-over contamination p. 5

Quality guarantee by conformity testing at regular intervals p. 6

Superior shielding with the special ShaftProtect 10µl tip p. 7

Filter tips – Pipetting small volumes from 0.1 – 20µl p. 8 - 9

Filter tips – Pipetting sample volumes from 1 – 1,250µl p. 10 - 11

TipStack Pack reload system p. 12 - 13

Tip SystemBox - Central pipetting station and reload trays p. 12

Tip StackPack / Starter Kit – the economic reload system p .13

Compatibility Table p. 14 - 17

Ordering information on quality pipette tips with and without filter p. 18 - 19

Robotic tips Tips for Biomek pipetting platforms p. 20

Multiply® reaction tubes PCR tubes, strips of 4 and 8 tubes p. 21 - 23

Product description of Multiply® PCR tubes, strips and lids p. 21

Ordering Information for Multiply® PCR tubes, strips and lids p. 22

Accessories: The interchangeable RackSystem for sample preparation p. 23

Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31

White PCR plates optimised for qPCR p. 24 - 26

Ordering information for white PCR plates and optical lids p. 26

Transparent PCR plates, w/o skirt, with half and full skirt p. 27 - 28

Ordering information for transparent PCR plates and lids p. 29

Compatibility Table p. 30 - 31

96 Deep well MegaBlock® Free from DNA, DNase/RNase and endotoxins p. 32

Adhesive sealing tapes For qPCR/PCR, automation and sample storage p. 33 - 35

Highly transparent, adhesive qPCR tape p. 33

Transparent tape for end point PCR/storage, strongly adhesive p. 34

Aluminium tape for PCR and sample storage p. 34

Pierceable tape for DNA sequencers, HPLC, autosamplers p. 35

SafeSeal micro tubes 0.5/1.5 and 2.0 ml with secure cap locking feature p. 36 - 37

UV transparent single-use cuvettes For photometric analyses ≥ 220 nm p. 38 - 39

1

AB

CD

EF

GH

23

45

67

89

10 1112

New

New

New

New

New

Page 4: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

4

Sarstedt quality symbols - a warranty of reliability and reproducibility

To accommodate the highly sophisticated needs of customers working in the field of medical research, environmental technology, the pharmaceutical and food industries as well as in cellular and molecular biology, Sarstedt has developed the Biosphere® quality products.The superior biological purity of sterile laboratory products is guaranteed by a controlled production process that complies with the strictest purity requirements so that our customers can be sure to achieve utmost reproducibility in their analyses and research experiments.

All disposables, such as pipette tips and reaction tubes bearing the Biosphere® symbol aresterile, DNA-, RNase- and ATP-free and non-pyrogenic.

To reduce storage time of the sterile Biosphere® products in open bags and inherent contamination risks, Biosphere® tubes and plates are packaged in small quantities.

Biosphere® laboratory products are manufacturedin accordance with defined production requirementsand meet the following threshold values:

DNA < 2 x 10-14 g RNase < 1.1 x 10-11 Kunitz unitsATP < 3.5 x 10-15 g Pyrogens < 2 x 10-3 EU

Sterility is validated according to ISO 11135.

PCR and other molecular-biological methods have gained access to laboratories all over the world. Sarstedt has enormously refined the production requirements for non-sterile PCR products. Skilled personnel, clean room conditions along with automated production processes enable contamination-free manufacturing.

Each production batch is subjected to a highly sensitive PCR method toguarantee compliance with the individual quality criteria.

Consequently, award of the PCR Performance Tested quality symbol to a production batch is subject to successfully passing PCR tests.Thanks to this quality grade, customers can use Sarstedt disposables in direct contact with reagents and sample material for PCR in their experimental work.

Products awarded the PCR Performance Tested standard meet the following requirements:

3 DNase-/RNase-free 3Human DNA-free 3PCR inhibitor-free

Page 5: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

5

Sarstedt quality symbols - a warranty of reliability and reproducibility High purity Biosphere® Filter tips for pipetting – free from carry-over contamination

Protection against carry-over and contaminationEven the most meticulous pipetting may generate aerosols during aspiration of the sample, contaminating the pipette and any sample material to follow. This cross-contamination cannot be tolerated when working with expensive, hazardous, or irreplaceable samples or when using highly sensitive methods. In order to prevent aerosol contamination along with the resulting consequences (falsified results, additional expenditure in time and cost) Sarstedt incorporates special porous elements in its range of filter tips.

Reliable safety with selected filter materials The filter element integrated in the pipette tips consists of a porous plastic material featuring defined hydrophobic properties and no self-sealing additives.

• The sponge-like material is densely composed of randomly arranged channels (see picture on left), enabling air to pass for precision when using air displacement pipettes.

• Simultaneously, the complex structure of the porous filter safely prevents aerosols from entering the pipette shaft.

Measuring the efficiency of filter inserts in Biosphere® filter tips*Both the PCR-based method and indirect, radioactive measurement of the plasmid DNA with P-32 marked ATP are reliable methods for detecting carry-over contamination of DNA aerosols likely to occur in practical pipetting applications.

• With both methods, the hydrophobic filter inserts with the selected porosity used in Sarstedt Biosphere® filter tips were found to provide protection against aerosol contamination.

• The superior efficiency of the filter in Biosphere® filter tips is based on a complex mechanism of absorption, surface quality, thickness, strength and shape.

* Refer to our “Application Report – Shielding Effect of Filter Inserts in Pipette Tips” for additional details

Added protection with Biosphere® purity quality3 Maximum biological purity: free from DNA, RNase, ATP and pyrogens

3 Batch-specific certification is available upon request

Convenient and safe packaging of individual tip boxes3 Each tip box is additionally and aseptically protected in a special sterile bag packaging

3 Easy opening of bag packaging without aid via notch at short bag side (see picture)

Cross section of a porous filter element (40-fold magnification)

piston

pipette shaft

aerosol

aerosol-free

filter

sample

sealing surfaceof pipette tip

Page 6: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

6

A calibrated system: Pipette and pipette tipsThe precision of the pipetting process is primarily determined by the optimal compatibility of the pipette and pipette tip.

• One of the leading manufacturers of pipette tips, Sarstedt’s main objective for years has been to provide customers with tips in optimal, reliable quality to fit most marketable pipette brands.

• The quality of a tip is defined by the properties of the material used, the design and optimum fit on the individual pipettes.

Quality guarantee: Precision and accuracy for small volumesIn particular, the ongoing trend towards ever decreasing small sample volumes inevitably involves increasing demands on the dosing system. At regular intervals, Sarstedt tips are checked for maximum precision and reliability in combination with the pipettes of leading manufacturers (e.g. Eppendorf, Gilson). In addition, the tips are subjected to a defined procedure of comprehensive in-process conformity testing in accordance with the international standard EN ISO 8655 for piston pipettes.

!

Note on availability of a

Declaration of Conformity for

pipette/pipette tip combinations

• The Declaration of Conformity for piston pipettes indicates the combination of pipette and pipette tip to which it applies attesting precision and reliability and can be made available on request.

• The logo on the label indicates that a standard Declaration of Conformity is available for a particular pipette tip.

The pipette tip – Crucial for precise dosing

Page 7: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

7

Safe, precise and accurate pipetting down to the base of 1.5/2.0 ml micro tubes with the ShaftProtect 10 µl tip for Gilson P2/P10

• Flexibility & convenient handling

With the dual position tip-ejector* (see illustration), the extended ShaftProtect tip covers as much of the pipette shaft as the short 70.1130.

ShaftProtect10µl tip

Standard10µl tip

non-sterilesterile

Dual position tip-ejector twisted by 180° (Pos. 1/Pos. 2)

in axial direction allowing choose between the long

70.1131 and short 70.1130 tips

180°

Pipette cone sealing position

Dua

l po

sitio

n tip

-eje

cto

r P

os.

1

Dua

l po

sitio

n

tip-e

ject

or P

os. 2

70.1131 70.1130

* All new (since 2003) P2/P10 pipettors from Gilson are equipped with dual position tip-ejector, allowing the use of short tip 70.1130.xxx and „Gilson long“ 70.1131.xxx. Older P2/P10 models need the Gilson ”Service Kit 2”.

Precise pipetting of micro volumes - superior precision and contamination protection with the ShaftProtect 10 µl tip for Gilson P2/P10With most extra long tips the dead volume is extended, which creates adverse effects on performance - but ShaftProtect 70.1131 has been especially developed for Gilson P2/P10 pipettors.

ShaftProtect

10µl

2µl

The pipette tip – Crucial for precise dosing

ShaftProtect 10 µl tip

Filter tip 70.1131.210 0.1 - 10 µl

• Extra-long tip with ShaftProtect for Gilson P2/P10 to protect the inner tube wall against the generally non-sterile pipette shaft

• Identical precision and accuracy thanks to a very small dead volume comparable to the short tip 70.1130

• Enhanced dosing features Especially fine, extra-long 'ShaftProtect' 10 µl tip for easy dosing of even the smallest volumes

• Easy visual control Calibration rings facilitate visual control for micro volumes

• Conformity-tested to EN ISO 8655

• Colour code

!

Page 8: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

8

Pipetting small sample volumes 0.1 – 10 µlWorking routinely with micro volumes can adversely affect the pipette / pipette tip dosing system. Therefore, tips to 10 µl feature the following product benefits of enhanced visual control and dosing:

• Enhanced pipetting safety due to calibrated system - conformity-tested to EN ISO 8655

• Easy visual control for micro volumes - calibration rings facilitate visual control of the pipetted volume

• Enhanced dosing features - fine, thin tip facilitates dosing and visual volume control

The pipette tip – Crucial for precise dosing

20 µl

10 µl

2 µl

70.1116.210

!

Filter tip 70.1116.210 0.5 - 20* µl

Universally compatible with Gilson, Eppendorf, or identical designs, e.g . Biohit, Finnpipette, Abimed and Brand for volumes between 0.1 and 10 µl. The fine, extra-long tip and calibration rings facilitate dosing and visual volume control.

* Compatible with Eppendorf Reference 2 - 20 µl and identical pipette models from other manufacturers.

• Conformity tested to EN ISO 8655

• Colour code

10 µl2 µl

70.1130.210/.212

!

Filter tip 70.1130.210/.212 0.1 - 10 µl / 0.1 - 2.5 µl

Mini Gilson tip for Gilson P2/P10 or Eppendorf Research 2.5 µl with finely drawn tip and calibration rings in 2 and 10 µl tips, as well as for versions of identical design from Finnpipette, Biohit and Socorex.

• Conformity tested to EN ISO 8655

• Colour code!

!

Page 9: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

9

Pipetting micro volumes from 0.5 to 20 µl – specially designed tip for perfect fit on Eppendorf pipettes Tip with ergonomical and flexible cone, enhanced sealing properties and outstanding precision on Eppendorf pipettes. The cone geometry enables optimal fit and effortless dosing with new generation single- or multi-channel pipettes (e.g. Research plus pipettes). The tip is also compatible with identical pipette models from other manufacturers.

The pipette tip – Crucial for precise dosing

70.1115.210

!

Filter tip 70.1114.210 0.5 to 20 µl

Compatible with 0.5 - 10 µl Eppendorf Research, Research plus, Research pro, Xplorer, Reference 2 - 20 µl and Finnpipette Digital 0.2 - 2 µl, as well as models of identical design.

• 46 mm tip length enables safe pipetting down to the bottom of deep vials

• Cone geometry optimised for 8- and 12-channel pipettes

• Graduation rings without interfering ridges for easy dosing into vials

• Extra thin tip for enhanced dosing of micro volumes

• Visual control of micro volumes Graduation rings at 2, 10 and 20 µl

• Conformity tested to EN ISO 8655

• Colour code!

Now available with perforated temper-evident closureNEW

Filter tip 70.1115.210 0.5 - 10 µl

Type “crystal tip” for Eppendorf 0.5 - 10 µl, Reference/Research or identical designs, e.g. Finnpipette.

• Conformity tested to EN ISO 8655

• Colour code

!

10µl

20µl

2µl

New

Page 10: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

10

Filter tip 70.765.210 10 - 300 µl

Specially suited for multi-channel pipettes, e.g. from Eppendorf, Brand, Finnpipette, Biohit. Also compatible with older multi-channel pipettes up to 200 µl.55.6 mm tip with 4 graduations for visual control of pre-set pipetting volume at 50, 100, 200 and 300 µl.

• Conformity tested to EN ISO 8655

• Colour code

200 µl

100 µl

70.765.210

50 µl

!

300 µl

70.760.212/.213

!

Filter tip 70.760.212/.213 2 - 100 µl

Yellow standard pipette tip for pipettes from Eppendorf, Gilson, Biohit, Brand, Socorex as well as identical designs, and additional filter tip option of 20 µl volume (70.760.213).

• Conformity tested to EN ISO 8655

• Colour code

Pipetting sample volumes 2 - 300 µl

70.760.211

!

Filter tip 70.760.211 2 - 200 µl

Standard pipette tip for pipettes from Eppendorf, Gilson, Biohit, Finnpipette, Socorex and identical designs.

• Conformity tested to EN ISO 8655

• Colour code

The pipette tip – Crucial to precise dosing

!

!

!

Page 11: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

11

Pipetting large sample volumes up to 1,250 µl

• Two pipette tips with graduation rings for volumes between 1 and 1,250 µl

• Saves space as a large volume range is covered with 2 tips only

• Colour-coded trays enable easy volume identification

• Highly transparent tip material for enhanced pipetting control

70.1186.210

1,000 µl

500 µl

200 µl

Filter tip 70.1186.210 50 - 1,250 µl, extra long

Extra long (103 mm) and slim pipette tip, universally suited for use with single-channel pipettes, e.g. from Gilson, Biohit, Nichyrio and Finnpipette. Particularly recommended for applications in Deep Well blocks, Cluster tubes or 5 ml CryoPure tubes for which the traditional 1,000 µl tip is too short or too thick. Compatible with multi-channel pipette Finnpipette Novus 100 to 1,200 µl. 3 Graduations for visual control of pre-set pipetting volume at 200, 500 and 1,000 µl.

- Empty box without pipette tips 95.1763, 8 pcs./case

• Colour code

70.762.211

!

Filter tip 70.762.211 50 - 1,000 µl

Blue standard tip for Eppendorf, Gilson, Finnpipette, Biohit, Brand, Socorex and identical designs.

- Empty box without pipette tips 95.1762, 10 pcs./case

• Conformity tested to EN ISO 8655

• Colour code

The pipette tip – Crucial to precise dosing

200 µl

50 µl

100 µl

Filter tip 70.1189.215* 1 - 200 µl, extra long

Extra long and slim 200 µl tip for 20, 50, 100 and 200 µl pipettes. Designed for universal use on a pipette volume range between 2 and 200 µl: Eppendorf Reference, Research, Biohit mLine, -Proline, -Proline Electronic, HTL, Labnet, Finnpipette, Nichiryo Benchmate, Gilson, Rainin, CappAero, CappTronic Electronic and identical designs.

- Soft, flexible cone for easy assembly and ejection- 77 mm length for reaching into tubes- One pipette accommodates 4 different volumes- Graduation rings without interfering ridges at 10, 50, 100 and 200 µl for visual control of the defined pipetting volume

• Colour code

70.1189.215

10 µl

NEW

!

* Expected September 2011

Page 12: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

12

Empty reload tray for use in the Tip SystemBoxUsers purchasing tips from our range loose in bags can load tips into empty trays (grey, yellow, orange) in the Tip SystemBox.

• Place empty trays into the Tip SystemBox and load with tips.

• Autoclave tips in the Tip SystemBox, if required. See important notes on autoclaving on page 20.

The Tip SystemBox: Central Pipetting Station

Simple and practical

Flexible and cost-effective

Empty 96-tip refill trays and bulk tipsSuitable for manually racking bulk tips for autoclaving and convenient handling.*

Economic and environmentally friendly

Tip StackPack Efficient handling of 96-tip trays, reduced packaging and storage space

Ordering Information empty reload trays

Coloured trays for easy tip volume identification

Order No. Tray colour code Tip type** / Volume Packaging (pcs./case)

95.1760.011 A, C / 200 µl 25

95.1760.022 D, J, N / 20 µl 25

95.1760.033 E long / 10 µl 25

95.1760.034 E / 10 µl 25

95.1760.044 L / 300 µl 20

* Important notes on autoclaving: See page 20** Pipette type see page 18 to 19

Tip SystemBox - Methodical tip processing

Page 13: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

13

* Important notes on autoclaving: See page 20

Ordering Information Tip StackPack Starter Kit

Order No. Volume Pipette tip colour Colour code Packaging/case

70.760.591 250 µl neutral3 x Tip StackPack

+1 x Tip SystemBox

70.760.592 200 µl yellow

70.1130.690 10 µl neutral

Tip StackPack Starter KitThe Tip StackPack Starter Kit contains 3 Tip StackPacks and 1 Tip SystemBox each

Tip StackPack reload systemTips are preloaded in stacked trays of 96 tips each, stacked on top of each other. The base section and cover are designed for multiple use in combination with the 5 or 6 tip trays. As a result, composite materials and storage space are reduced by more than 50%.

• Space-saving

• Easy to use

• Reduced waste volumes

• Tip StackPack Starter Kit (3 x Tip StackPack + 1 Tip SystemBox)

Ordering Information for Tip StackPack see page 19

Economic and environmentally friendly• Easy transfer of individual layers of 96-tip trays into the Tip SystemBox for easy handling or autoclaving in the pipette tip box*

• Safety labels applied on both sides can be easily perforated to access the next layer of tips

• Trays can be placed into the Tip SystemBox without further adjustment

• Tips can also be used directly from the stack

Ordering Information Tip SystemBox

Order No. Description Packagingpcs./case

95.1298.001Tip SystemBox (without Pipette tips),

empty box6

Safety labels appliedon both sides can

be easily perforated to access the next

layer of 96 tips

Tip SystemBox - Methodical tip processing

Page 14: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

14

Biosphere®-Pipette Tips with filter (illustrations 60%)

Eppendorf Pipettes Thermo Finnpipettes Gilson Pipettes

Eppendorf Reference Eppendorf Research Eppendorf Research plus Eppendorf Research pro Xplo-rer Finnpipette Digital / F1 / F2 Finnpipette Focus Finnpipette Novus Bio

Control Pipetman / Ultra / Concept

0.1-

2.5

µl

0.5-

10 µ

l

2-20

µl

2-20

µl

10-1

00 µ

l

50-2

00 µ

l

100-

1,00

0 µl

0.1-

2.5

µl

0.5-

10 µ

l

2-20

µl

10-1

00 µ

l

20-2

00 µ

l

30-3

00 µ

l MC

100-

1,00

0 µl

500-

5,00

0 µl

1-10

ml

0.5-

10 µ

l MC

2-20

µl

10-1

00 µ

l

20-2

00 µ

l

30-3

00 µ

l MC

100-

1,00

0 µl

500-

5,00

0 µl

1-10

ml

0.5-

10 µ

l

0.5-

10 µ

l MC

5-10

0 µl

5-10

0 µl

MC

20-3

00 µ

l

20-3

00 µ

l MC

50-1

,000

µl

50-1

,200

µl M

C

100-

5,00

0 µl

0.5-

10 µ

l MC

0.2-

2 µl

F2 0

.5-5

µl

F1 1

-10

µl

0.5-

10 µ

l Uni

v.

0.5-

10 µ

l MC

2-20

µl U

niv.

5-50

µl M

C

10-1

00 µ

l Univ

.

20-2

00 µ

l Univ

.

50-3

00 µ

l MC

100-

1,00

0 µl

200-

1,00

0 µl

0.3-

3 µl

0.5-

5 µl

1-10

µl

30-3

00 µ

l

100-

1,00

0 µl

30-3

00 µ

l

30-3

00 µ

l MC

100-

1,00

0 µl

100-

1,20

0 µl

MC

50-3

00 µ

l MC

P2/U

2 0.

2-2

µl

P10/U

10 0

.1-10

µl

C8/C

12 1

-10

µl

P20/

U20

2-20

µl

P100

/U10

020

-100

µl

P200

/U20

020

-200

µl

U8 2

0-30

0 µl

P100

0/U1

000

200-

1,00

0 µl

P5,0

00/U

5,00

01,

000-

5,00

0 µl

P10/

U10

1-10

ml

2.5

µl

70.1130.212 3 32.5

32.5 3 3

2.532.5

32.5

32.5 3 3

2.532.5 3 3

2.532.5 3 3

2.5 ✗

10 µ

l 70.1130.21070.1130.410 3 3

10 ✗ 3 3 3 3 3 3 3 3 3 3 3 ✗

10 µ

l

70.1131.210 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ 3 3 3

10 µ

l 70.1115.21070.1115.410 3 3 3

10 3 3 3 3 3 3 3 3 3 ✗ ✗ ✗

20 µ

l 70.1114.21070.1114.410 ✗ 3 3 ✗ 3 3 3 3 3 3 3 3 3 3 3 3 3

20 µ

l

70.1116.210 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 ✗

20 µ

l 70.760.21370.760.413 3 3

20320 ✗ 3

20320 ✗ 3 3

20 3 ✗ 320

320 3 3

20 ✗ 3 320

100

µl 70.760.21270.760.412 3 3 3

100 3 3 3100 ✗ 3 3 3

100 ✗ 3 3 3 3100

3100 ✗ ✗ 3

200

µl

70.1189.215 3 3 3 3 3 3 3 33 3 3 33 3 3 3

200

µl 70.760.21170.760.411 3 3 3 3 ✗ 3 ✗ 3

2003200 ✗ ✗ 3 3

2003200

3200 ✗ ✗ 3 ✗

300

µl

70.765.210 ✗ ✗ 3 ✗ ✗ 3 3 3 3 3 3 ✗ ✗ 3 3 3 3 ✗ ✗ 3 3

1,00

0 µl

70.762.21170.762.411 3 3 3 3 ✗ 3 3 3 3 3

1,25

0 µl

70.1186.21070.1186.410 3 3 3 3 3 3 3 3 3 3

Compatibility Table - Pipette Tips

Quality Pipette Tips without filter* (illustrations 60%) Eppendorf Reference Eppendorf Research Eppendorf Research plus Eppendorf Research pro Xplo-rer Finnpipette Digital / F1 / F2 Finnpipette Focus Finnpipette Novus Bio

Control Pipetman / Ultra / Concept

10 µ

l 70.113070.1130.10070.1130.20070.1130.600

3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 ✗

10 µ

l 70.113170.1131.100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ 3 3 3

20 µ

l 70.111470.1114.10070.1114.200

✗ 3 3 ✗ 3 3 3 3 3 3 3 3 3 3 3 3 3 3

20 µ

l 70.111570.1115.10070.1115.200

3 3 3 3 3 3 3 3 3 3 3 3 3 ✗

20 µ

l 70.111670.1116.10070.1116.200

3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 ✗

200

µl

70.760.00270.760.10270.760.20270.760.502

3 3 3 3 3 3 ✗ 3 3 3 ✗ 3 3 3 3 3 3200 ✗ 3 3 3

200

200

µl

70.1189.105 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3

250

µl 70.760.00170.760.20170.760.501

3 3 3 3 ✗ 3 ✗ 3250

3250 ✗ ✗ 3 3

250 ✗ ✗ 3 3250

300

µl 70.76570.765.10070.765.200

✗ ✗ 3 ✗ ✗ 3 3 ✗ ✗ 3 3 ✗ ✗ 3 3 ✗ ✗ 3 3 3 3 3 3 ✗ ✗ 3 3

1,00

0 µl 70.762

70.762.10070.762.200

3 3 3 3 ✗ 3 3 3 3 ✗ 3

1,25

0 µl 70.1186

70.1186.10070.1186.200

3 3 3 3 3 3 3 3 3 3

5 m

l

Scale 1:2

70.1183.00170.1183.101 ✗ ✗ ✗ 3

5 m

l

Scale 1:2

70.1183.00270.1183.102 3 3 3 ✗

10 m

l

Scale 1:2

70.1187.00170.1187.101 3 3 3

NEW

NEW

NEW

NEW

Legend: = max. pipetting volume

= limited pipetting volume

= not recommended

310✗

= not checked

MC = Multi-channel pipette

Page 15: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

15

Biosphere®-Pipette Tips with filter (illustrations 60%)

Eppendorf Pipettes Thermo Finnpipettes Gilson Pipettes

Eppendorf Reference Eppendorf Research Eppendorf Research plus Eppendorf Research pro Xplo-rer Finnpipette Digital / F1 / F2 Finnpipette Focus Finnpipette Novus Bio

Control Pipetman / Ultra / Concept

0.1-

2.5

µl

0.5-

10 µ

l

2-20

µl

2-20

µl

10-1

00 µ

l

50-2

00 µ

l

100-

1,00

0 µl

0.1-

2.5

µl

0.5-

10 µ

l

2-20

µl

10-1

00 µ

l

20-2

00 µ

l

30-3

00 µ

l MC

100-

1,00

0 µl

500-

5,00

0 µl

1-10

ml

0.5-

10 µ

l MC

2-20

µl

10-1

00 µ

l

20-2

00 µ

l

30-3

00 µ

l MC

100-

1,00

0 µl

500-

5,00

0 µl

1-10

ml

0.5-

10 µ

l

0.5-

10 µ

l MC

5-10

0 µl

5-10

0 µl

MC

20-3

00 µ

l

20-3

00 µ

l MC

50-1

,000

µl

50-1

,200

µl M

C

100-

5,00

0 µl

0.5-

10 µ

l MC

0.2-

2 µl

F2 0

.5-5

µl

F1 1

-10

µl

0.5-

10 µ

l Uni

v.

0.5-

10 µ

l MC

2-20

µl U

niv.

5-50

µl M

C

10-1

00 µ

l Univ

.

20-2

00 µ

l Univ

.

50-3

00 µ

l MC

100-

1,00

0 µl

200-

1,00

0 µl

0.3-

3 µl

0.5-

5 µl

1-10

µl

30-3

00 µ

l

100-

1,00

0 µl

30-3

00 µ

l

30-3

00 µ

l MC

100-

1,00

0 µl

100-

1,20

0 µl

MC

50-3

00 µ

l MC

P2/U

2 0.

2-2

µl

P10/U

10 0

.1-10

µl

C8/C

12 1

-10

µl

P20/

U20

2-20

µl

P100

/U10

020

-100

µl

P200

/U20

020

-200

µl

U8 2

0-30

0 µl

P100

0/U1

000

200-

1,00

0 µl

P5,0

00/U

5,00

01,

000-

5,00

0 µl

P10/

U10

1-10

ml

2.5

µl

70.1130.212 3 32.5

32.5 3 3

2.532.5

32.5

32.5 3 3

2.532.5 3 3

2.532.5 3 3

2.5 ✗

10 µ

l 70.1130.21070.1130.410 3 3

10 ✗ 3 3 3 3 3 3 3 3 3 3 3 ✗

10 µ

l

70.1131.210 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ 3 3 3

10 µ

l 70.1115.21070.1115.410 3 3 3

10 3 3 3 3 3 3 3 3 3 ✗ ✗ ✗

20 µ

l 70.1114.21070.1114.410 ✗ 3 3 ✗ 3 3 3 3 3 3 3 3 3 3 3 3 3

20 µ

l

70.1116.210 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 ✗

20 µ

l 70.760.21370.760.413 3 3

20320 ✗ 3

20320 ✗ 3 3

20 3 ✗ 320

320 3 3

20 ✗ 3 320

100

µl 70.760.21270.760.412 3 3 3

100 3 3 3100 ✗ 3 3 3

100 ✗ 3 3 3 3100

3100 ✗ ✗ 3

200

µl

70.1189.215 3 3 3 3 3 3 3 33 3 3 33 3 3 3

200

µl 70.760.21170.760.411 3 3 3 3 ✗ 3 ✗ 3

2003200 ✗ ✗ 3 3

2003200

3200 ✗ ✗ 3 ✗

300

µl

70.765.210 ✗ ✗ 3 ✗ ✗ 3 3 3 3 3 3 ✗ ✗ 3 3 3 3 ✗ ✗ 3 3

1,00

0 µl

70.762.21170.762.411 3 3 3 3 ✗ 3 3 3 3 3

1,25

0 µl

70.1186.21070.1186.410 3 3 3 3 3 3 3 3 3 3

Quality Pipette Tips without filter* (illustrations 60%) Eppendorf Reference Eppendorf Research Eppendorf Research plus Eppendorf Research pro Xplo-rer Finnpipette Digital / F1 / F2 Finnpipette Focus Finnpipette Novus Bio

Control Pipetman / Ultra / Concept

10 µ

l 70.113070.1130.10070.1130.20070.1130.600

3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 ✗

10 µ

l 70.113170.1131.100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ 3 3 3

20 µ

l 70.111470.1114.10070.1114.200

✗ 3 3 ✗ 3 3 3 3 3 3 3 3 3 3 3 3 3 3

20 µ

l 70.111570.1115.10070.1115.200

3 3 3 3 3 3 3 3 3 3 3 3 3 ✗

20 µ

l 70.111670.1116.10070.1116.200

3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 ✗

200

µl

70.760.00270.760.10270.760.20270.760.502

3 3 3 3 3 3 ✗ 3 3 3 ✗ 3 3 3 3 3 3200 ✗ 3 3 3

200

200

µl

70.1189.105 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3

250

µl 70.760.00170.760.20170.760.501

3 3 3 3 ✗ 3 ✗ 3250

3250 ✗ ✗ 3 3

250 ✗ ✗ 3 3250

300

µl 70.76570.765.10070.765.200

✗ ✗ 3 ✗ ✗ 3 3 ✗ ✗ 3 3 ✗ ✗ 3 3 ✗ ✗ 3 3 3 3 3 3 ✗ ✗ 3 3

1,00

0 µl 70.762

70.762.10070.762.200

3 3 3 3 ✗ 3 3 3 3 ✗ 3

1,25

0 µl 70.1186

70.1186.10070.1186.200

3 3 3 3 3 3 3 3 3 3

5 m

l

Scale 1:2

70.1183.00170.1183.101 ✗ ✗ ✗ 3

5 m

l

Scale 1:2

70.1183.00270.1183.102 3 3 3 ✗

10 m

l

Scale 1:2

70.1187.00170.1187.101 3 3 3

Pipette suppliers occasionally rework or carry out technical modifications to the pipette cones of their pipettors so that the information provided in the Compatibility Table constitutes a recommendation only. In case of doubt, free sample tips are available on request to test the optimum fitting and ejection behaviour of our tips when used on your pipettes.

* Important notes on autoclaving see page 20

Page 16: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

16

Quality Pipette Tips without filter* (illustrations 60%) mLine Mechanical Proline Mechanical eLine Electronic Transferpette Digital Transferpette S Transferpette Electronic Calibra Manual Micro 822 C. M. Multi 852 Acura Manual Micro 825Acura Electro Micro 925

A. 835/935e

Acura Multi 855/955e

10 µ

l 70.113070.1130.10070.1130.20070.1130.600

3 3 3 3 3 3 3 3 ✗ 3 3 3 3 3 3 3 3 3 3 3 3

10 µ

l 70.113170.1131.100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗

20 µ

l 70.111470.1114.10070.1114.200

3 3 3 3

20 µ

l 70.111570.1115.10070.1115.200

✗ ✗ 3 3 ✗ 3 3 3 3 3 3 3 3 ✗ 3 3 ✗

20 µ

l 70.111670.1116.10070.1116.200

3 3 3 3 3 ✗ 3 3 ✗ 3 3 3 3 3 3 3 3 ✗ 3 3

200

µl

70.760.00270.760.10270.760.20270.760.502

✗ 3 ✗ 3 3 ✗ ✗ ✗ ✗ 3 3 3 ✗ ✗ 3 3 3 ✗ 3 3 3 3 3 3 3 3 3 3 3 3 3 3

200

µl

70.1189.105 3 3 3 3 3 3

250

µl 70.760.00170.760.20170.760.501

✗ 3 3 3 3 3 3250 ✗ 3 3 3 ✗ ✗ 3 3 3 ✗ 3 ✗ ✗ ✗ 3 ✗ ✗ ✗ ✗ ✗ ✗ 3 ✗ 3

300

µl 70.76570.765.10070.765.200

✗ 3 3 ✗ ✗ 3 3 3 3 ✗ ✗ ✗ 3 3 ✗ ✗ 3 ✗ 3 ✗ ✗ ✗ 3 ✗ ✗ ✗ ✗ 3 3 3 ✗ 3

1,00

0 µl 70.762

70.762.10070.762.200

3 3 3 ✗ 3 3 3 3 3 3

1,25

0 µl 70.1186

70.1186.10070.1186.200

3 3 ✗ 3 3 ✗ 3 3 3

5 m

l

Scale 1:2

70.1183.00170.1183.101 ✗ ✗ ✗ ✗

5 m

l

Scale 1:2

70.1183.00270.1183.102 3 3 3 3

10 m

l

Scale 1:2

70.1187.00170.1187.101 3 ✗

Biosphere®-Pipette Tips with filter (illustrations 60%)

Biohit Pipettes Brand Pipettes Socorex Manual / Electronic Pipettes

mLine Mechanical Proline Mechanical eLine Electronic Transferpette Digital Transferpette S Transferpette Electronic Calibra Manual Micro 822 C. M. Multi 852 Acura Manual Micro 825Acura Electro Micro 925

A. 835/935e

Acura Multi 855/955e

0.5-

10 µ

l

0.5-

10 µ

l MC

2-20

µl

10-1

00 µ

l

20-2

00 µ

l

30-3

00 µ

l MC

100-

1,00

0 µl

100-

5,00

0 µl

0.1-

2.5

µl

0.5-

10 µ

l

5-50

µl

5-50

µl M

C

20-2

00 µ

l

50-2

50 µ

l MC

50-3

00 µ

l MC

100-

1,00

0 µl

100-

5,00

0 µl

0.2-

10 µ

l

10-3

00 µ

l

50-1

,000

µl

50-1

,200

µl M

C

100-

5,00

0 µl

0.1-

1 µl

0.5-

10 µ

l

2-20

µl

2-20

µl

5-50

µl

10-1

00 µ

l

20-2

00 µ

l

30-3

00 µ

l MC

25-2

50 µ

l

100-

1,00

0 µl

0.1-

1 µl

0.5-

10 µ

l

0.5-

10 µ

l MC

2-20

µl

10-1

00 µ

l

20-2

00 µ

l

30-3

00 µ

l MC

100-

1,00

0 µl

1-10

ml

0.5-

10 µ

l

0.5-

10 µ

l MC

2-20

µl

1-20

µl M

C

20-2

00 µ

l

100-

1,00

0 µl

0.2-

2 µl

1-10

µl

1-10

µl

2-20

µl

10-1

00 µ

l

20-2

00 µ

l

100-

1,00

0 µl

1-10

µl

10-1

00 µ

l

20-2

00 µ

l

0.1-

2 µl

0.5-

10 µ

l

1-10

µl

2-20

µl

5-50

µl

10-1

00 µ

l

10-2

00 µ

l

100-

1,00

0 µl

0.5-

5 m

l

0.5-

10 µ

l MC

5-50

µl M

C

20-2

00 µ

l MC

2.5

µl

70.1130.212 32.5

32.5 3 3

2.532.5 ✗ 3

2.532.5 ✗ 3

2.532.5

32.5

32.5

32.5

32.5 3 3

2.532.5 3 ✗ 3

2.5

10 µ

l 70.1130.21070.1130.410 3 3 3 3 ✗ 3 3

10 ✗ 3 3 3 3 3 3 3 3 3 3 3 3

10 µ

l

70.1131.210 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗

10 µ

l 70.1115.21070.1115.410 ✗ ✗ 3 3

10 ✗ 3 3 3 3 310310 3 3 ✗ 3 3 ✗

20 µ

l 70.1114.21070.1114.410 3 3 3 ✗ 3 3 3 3

20 µ

l

70.1116.210 3 3 3 3 3 ✗ 3 3 ✗ 3 3 3 3 3 3 ✗ ✗ ✗ 3 3

20 µ

l 70.760.21370.760.413 ✗ ✗ ✗ 3

20320 ✗ ✗ ✗ ✗ 3 3 3

20 ✗ ✗ 3 320320 ✗ 3

20310 3 3

20 ✗ 3 ✗ 3 3 320320 ✗ 3 ✗

100

µl 70.760.21270.760.412 ✗ 3

100 3 3 3100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ 3

100 ✗3100 ✗ ✗ ✗ 3

100 ✗ 3 ✗ ✗ 3 3 3100 3 3

100

200

µl

70.1189.215 3 3 3 3 3 3 3 3 3

200

µl 70.760.21170.760.411 ✗ 3 ✗ ✗ 3 3

2003200 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ 3 ✗ 3

100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ 3 ✗ 3

300

µl

70.765.210 ✗ 3 3 ✗ ✗ 3 3 3 3 ✗ ✗ ✗ 3 3 ✗ ✗ 3 ✗ 3 ✗ ✗ ✗ 3 ✗ ✗ ✗ ✗ ✗ ✗ 3 ✗ 3

1,00

0 µl

70.762.21170.762.411 3 3 3 ✗ ✗ 3 3 3 3 3

1,25

0 µl

70.1186.21070.1186.410 3 3 ✗ ✗ 3 3 3 3 3

Compatibility Table - Pipette Tips

NEW

NEW

NEW

NEW

Legend: = max. pipetting volume

= limited pipetting volume

= not recommended

310✗

= not checked

MC = Multi-channel pipette

Page 17: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

17

Quality Pipette Tips without filter* (illustrations 60%) mLine Mechanical Proline Mechanical eLine Electronic Transferpette Digital Transferpette S Transferpette Electronic Calibra Manual Micro 822 C. M. Multi 852 Acura Manual Micro 825Acura Electro Micro 925

A. 835/935e

Acura Multi 855/955e

10 µ

l 70.113070.1130.10070.1130.20070.1130.600

3 3 3 3 3 3 3 3 ✗ 3 3 3 3 3 3 3 3 3 3 3 3

10 µ

l 70.113170.1131.100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗

20 µ

l 70.111470.1114.10070.1114.200

3 3 3 3

20 µ

l 70.111570.1115.10070.1115.200

✗ ✗ 3 3 ✗ 3 3 3 3 3 3 3 3 ✗ 3 3 ✗

20 µ

l 70.111670.1116.10070.1116.200

3 3 3 3 3 ✗ 3 3 ✗ 3 3 3 3 3 3 3 3 ✗ 3 3

200

µl

70.760.00270.760.10270.760.20270.760.502

✗ 3 ✗ 3 3 ✗ ✗ ✗ ✗ 3 3 3 ✗ ✗ 3 3 3 ✗ 3 3 3 3 3 3 3 3 3 3 3 3 3 3

200

µl

70.1189.105 3 3 3 3 3 3

250

µl 70.760.00170.760.20170.760.501

✗ 3 3 3 3 3 3250 ✗ 3 3 3 ✗ ✗ 3 3 3 ✗ 3 ✗ ✗ ✗ 3 ✗ ✗ ✗ ✗ ✗ ✗ 3 ✗ 3

300

µl 70.76570.765.10070.765.200

✗ 3 3 ✗ ✗ 3 3 3 3 ✗ ✗ ✗ 3 3 ✗ ✗ 3 ✗ 3 ✗ ✗ ✗ 3 ✗ ✗ ✗ ✗ 3 3 3 ✗ 3

1,00

0 µl 70.762

70.762.10070.762.200

3 3 3 ✗ 3 3 3 3 3 3

1,25

0 µl 70.1186

70.1186.10070.1186.200

3 3 ✗ 3 3 ✗ 3 3 3

5 m

l

Scale 1:2

70.1183.00170.1183.101 ✗ ✗ ✗ ✗

5 m

l

Scale 1:2

70.1183.00270.1183.102 3 3 3 3

10 m

l

Scale 1:2

70.1187.00170.1187.101 3 ✗

Biosphere®-Pipette Tips with filter (illustrations 60%)

Biohit Pipettes Brand Pipettes Socorex Manual / Electronic Pipettes

mLine Mechanical Proline Mechanical eLine Electronic Transferpette Digital Transferpette S Transferpette Electronic Calibra Manual Micro 822 C. M. Multi 852 Acura Manual Micro 825Acura Electro Micro 925

A. 835/935e

Acura Multi 855/955e

0.5-

10 µ

l

0.5-

10 µ

l MC

2-20

µl

10-1

00 µ

l

20-2

00 µ

l

30-3

00 µ

l MC

100-

1,00

0 µl

100-

5,00

0 µl

0.1-

2.5

µl

0.5-

10 µ

l

5-50

µl

5-50

µl M

C

20-2

00 µ

l

50-2

50 µ

l MC

50-3

00 µ

l MC

100-

1,00

0 µl

100-

5,00

0 µl

0.2-

10 µ

l

10-3

00 µ

l

50-1

,000

µl

50-1

,200

µl M

C

100-

5,00

0 µl

0.1-

1 µl

0.5-

10 µ

l

2-20

µl

2-20

µl

5-50

µl

10-1

00 µ

l

20-2

00 µ

l

30-3

00 µ

l MC

25-2

50 µ

l

100-

1,00

0 µl

0.1-

1 µl

0.5-

10 µ

l

0.5-

10 µ

l MC

2-20

µl

10-1

00 µ

l

20-2

00 µ

l

30-3

00 µ

l MC

100-

1,00

0 µl

1-10

ml

0.5-

10 µ

l

0.5-

10 µ

l MC

2-20

µl

1-20

µl M

C

20-2

00 µ

l

100-

1,00

0 µl

0.2-

2 µl

1-10

µl

1-10

µl

2-20

µl

10-1

00 µ

l

20-2

00 µ

l

100-

1,00

0 µl

1-10

µl

10-1

00 µ

l

20-2

00 µ

l

0.1-

2 µl

0.5-

10 µ

l

1-10

µl

2-20

µl

5-50

µl

10-1

00 µ

l

10-2

00 µ

l

100-

1,00

0 µl

0.5-

5 m

l

0.5-

10 µ

l MC

5-50

µl M

C

20-2

00 µ

l MC

2.5

µl

70.1130.212 32.5

32.5 3 3

2.532.5 ✗ 3

2.532.5 ✗ 3

2.532.5

32.5

32.5

32.5

32.5 3 3

2.532.5 3 ✗ 3

2.5

10 µ

l 70.1130.21070.1130.410 3 3 3 3 ✗ 3 3

10 ✗ 3 3 3 3 3 3 3 3 3 3 3 3

10 µ

l

70.1131.210 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗

10 µ

l 70.1115.21070.1115.410 ✗ ✗ 3 3

10 ✗ 3 3 3 3 310310 3 3 ✗ 3 3 ✗

20 µ

l 70.1114.21070.1114.410 3 3 3 ✗ 3 3 3 3

20 µ

l

70.1116.210 3 3 3 3 3 ✗ 3 3 ✗ 3 3 3 3 3 3 ✗ ✗ ✗ 3 3

20 µ

l 70.760.21370.760.413 ✗ ✗ ✗ 3

20320 ✗ ✗ ✗ ✗ 3 3 3

20 ✗ ✗ 3 320320 ✗ 3

20310 3 3

20 ✗ 3 ✗ 3 3 320320 ✗ 3 ✗

100

µl 70.760.21270.760.412 ✗ 3

100 3 3 3100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ 3

100 ✗3100 ✗ ✗ ✗ 3

100 ✗ 3 ✗ ✗ 3 3 3100 3 3

100

200

µl

70.1189.215 3 3 3 3 3 3 3 3 3

200

µl 70.760.21170.760.411 ✗ 3 ✗ ✗ 3 3

2003200 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ 3 ✗ 3

100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ 3 ✗ 3

300

µl

70.765.210 ✗ 3 3 ✗ ✗ 3 3 3 3 ✗ ✗ ✗ 3 3 ✗ ✗ 3 ✗ 3 ✗ ✗ ✗ 3 ✗ ✗ ✗ ✗ ✗ ✗ 3 ✗ 3

1,00

0 µl

70.762.21170.762.411 3 3 3 ✗ ✗ 3 3 3 3 3

1,25

0 µl

70.1186.21070.1186.410 3 3 ✗ ✗ 3 3 3 3 3

Pipette suppliers occasionally rework or carry out technical modifications to the pipette cones of their pipettors so that the information provided in the Compatibility Table constitutes a recommendation only. In case of doubt, free sample tips are available on request to test the optimum fitting and ejection behaviour of our tips when used on your pipettes.

* Important notes on autoclaving see page 20

Page 18: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

18

Filter tips(illustrations 1:1) Order No.

Max. SampleVolume/Colour

QualityGrade Packaging

Type E70.1130.212 2.5 µl, neutral

96/box480/inner box

1,920/case

Type E70.1130.210 10 µl, neutral

96/box480/inner box

1,920/case

70.1130.410 10 µl, neutral480/bag (loose)

4,800/case

Type E long 70.1131.210 10 µl, neutral

96/box480/inner box

1,920/case

Type D70.1115.210 10 µl, neutral

96/box480/inner box

1,920/case

70.1115.410 10 µl, neutral480/bag (loose)

4,800/case

Type N70.1114.210 20 µl, neutral

96/box480/inner box

1,920/case

70.1114.410 20 µl, neutral480/bag (loose)

4,800/case

Type J70.1116.210 20 µl, neutral

96/box480/inner box

1,920/case

Type A70.760.213 20 µl, yellow

96/box480/inner box

1,920/case

70.760.413 20 µl, yellow480/bag (loose)

4,800/case

Type A70.760.212 100 µl, yellow

96/box480/inner box

1,920/case

70.760.412 100 µl, yellow480/bag (loose)

4,800/case

Type Oextra long 70.1189.215* 200 µl, neutral 96/box

960/case

Type C70.760.211 200 µl, neutral

96/box480/inner box

1,920/case

70.760.411 200 µl, neutral480/bag (loose)

4,800/case

Type L70.765.210 300 µl, neutral

96/box480/inner box

1,920/case

Type B70.762.211 1,000 µl, blue

100/box500/inner box

1,000/case

70.762.411 1,000 µl, blue200/bag (loose)

2,000/case

Type M70.1186.210

1,250 µl, neutral

96/box768/case

70.1186.4101,250 µl, neutral

384/bag (loose)3,840/case

Ordering Information - Quality tips with filter

* Expected September 2011

NEW

NEW

Page 19: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

19

Ordering Information - Quality tips with filter

* Expected September 2011

Refer to pp. 14 to 17 or see Catalogue for further information

Ordering Information - Quality tips without filter

Tips without filter(illustrations 1:1) Order No.

Max. SampleVolume/Colour

QualityGrade Packaging

Type E 70.1130

10 µl, neutral

1,000/bag, 10,000/case

70.1130.100 96/box, 1,920/case

70.1130.200 96/box, 480/inner case, 1,920/case

70.1130.600 Tip StackPack96/tray, 576/StackPack,

2,304/case70.1130.460

70.1130.690 Tip StackPack Starter Kit

Type E long 70.1131 10 µl, neutral

500/bag, 10,000/case

70.1131.100 96/box, 1,920/case

Type D 70.111510 µl, neutral

1,000/bag, 10,000/case

70.1115.100 96/box, 1,920/case

70.1115.200 96/box, 480/inner case, 1,920/case

Type N 70.111420 µl, neutral

1,000/bag, 10,000/case

70.1114.100 96/box, 1,920/case

70.1114.200 96/box, 480/inner case, 1,920/case

Type J 70.111620 µl, neutral

1,000/bag, 10,000/case

70.1116.100 96/box, 1,920/case

70.1116.200 96/box, 480/inner case, 1,920/case

Type A 70.760.002

200 µl, yellow

500/bag, 10,000/case

70.760.102 96/box, 1,920/case

70.760.202 96/box, 480/inner case, 1,920/case

70.760.502 Tip StackPack96/tray, 480/StackPack,

1,920/case70.760.452

70.760.592 Tip StackPack Starter Kit

Type O extra long 70.1189.105*

200 µl, neutral

96/box, 960/case

Type C 70.760.001

250 µl, neutral

500/bag, 10,000/case

70.760.201 96/box, 480/inner case, 1,920/case

70.760.501 Tip StackPack96/tray, 480/StackPack,

1,920/case70.760.451

70.760.591 Tip StackPack Starter Kit

Type L 70.765300 µl, neutral

500/bag, 5,000/case

70.765.100 96/box, 1,920/case

70.765.200 96/box, 480/inner case, 1,920/case

Type B 70.7621,000 µl,

blue

250/bag, 5,000/case

70.762.100 100/box, 1,000/case

70.762.200 100/box, 500/inner case, 1,000/case

Type M 70.11861,250 µl, neutral

384/bag, 3,840/case

70.1186.100 96/box, 3,840/case

70.1186.200 96/box, 768/case

Scale 1:2

70.1183.0015 ml

250/bag, 2,500/case

70.1183.101 50/Box, 500/case

Scale 1:2

70.1183.0025 ml

250/bag, 2,500/case

70.1183.102 50/box, 500/case

Scale 1:2

70.1187.00110 ml

200/bag, 2,000/case

70.1187.101 25/box, 250/case

NEW

NEW

Page 20: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

20

Pipettenspitzen für Biomek Pipettierroboter

The quality solution concept for automated liquid handling• Each production batch is tested for functionality and precision on original robotic instruments to ensure a consistently superior product quality

• All tips are certified DNase- and human DNA-free

• Sterile tips are also tested to be non-pyrogenic

• The coloured trays enable easy identification of tips and volumes

Important notes on autoclaving:

Products made from PP can be autoclaved up to 121°C without significantly impairing their mechanical properties. Users are to test for

themselves if autoclaving may have any effect on other characteristic product features so as to influence the individual application concerned.

NEW: Pipette tips for Biomek FX/NX · 3000

Product / Volume Colour code,tray

Tip with filter,sterile

Tip without filter,non-sterile

Tip without filter,sterile Packaging

70.1140.212* 70.1140.102* 70.1140.202**96/rack

*4,800/case**9,600/case

70.1141.212** 70.1141.102* 70.1141.202*96/rack

*4,800/case**9,600/case

70.1142.212* 70.1142.102* 70.1142.202**96/rack

*4,800/case**9,600/case

NEW: Pipette tips for Biomek 1000 · 2000

Product / Volume Colour code,tray

Tip with filter,sterile

Tip without filter,non-sterile

Tip without filter,sterile Packaging

70.1140.211* 70.1140.101** 70.1140.201**96/rack

*4,800/case**9,600/case

70.1141.211 70.1141.101 70.1141.20196/rack

9,600/case

70.1142.211 70.1142.101 70.1142.20196/rack

9,600/case

---- 70.1143.101 70.1143.20172/rack

3,600/case

20 µl

20 µl

35 µl

50 µl

175 µl

250 µl

1,000 µl

20 µl

20 µl

35 µl

50 µl

175 µl

250 µl

Pipette tips for Biomek pipetting platforms

Page 21: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

21

Pipette tips for Biomek pipetting platforms Reliable quality – Multiply® reaction tubes for PCR

The original Multiply® tubes are the result of consistently improving the design of the more traditional reaction tubes. Enhanced design features of the Multiply® tubes ensure work is risk-free from contamination and non-tiring compared to traditional tubes.

• Additional benefits of the Multiply® product range:• On request, we also offer conveniently packaged small quantities of products certified to the sterile Biosphere®, or ‘PCR Performance Tested’ quality standard

• Variety of options

• Optimum heat transfer thanks to thin-walled reaction space

• Compatible on thermocyclers with 0.2 and 0.5 ml block formats

• Enables micro centrifugation in most commonly used micro centrifuges (with adapter, as required)

• Integral anti-contamination shield: Preventsaccidentalcontactwiththeinnersurfaceofthelid Easy to open: Anti-contamination shield prevents potential contamination.

• Space-saving lid design: Frosted,flatlidwithlargewritingarea The frosted surface prevents smearing even when processed in a thermocycler. The space-saving lid design allows complete utilization of the heating block capacity.

• Interchangeable RackSystem for 0.2 ml tubes: Samplepreparation/storageintheRackSystempipettingstation Small recess at the skirt of the Multiply® PCR tubes allows tubes to be easily removed from the work tray. The small, roughened recess can also be used as an additional writing area. Description for RackSystem see page 23

Page 22: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

22

Ordering Information – Multiply® PCR Tubes

PCR single tubes

Product Order No. Volume Description Sterile Colour Quality Grade

Pieces per bag Packaging

72.735.100

0.5 ml

Biosphere® Multiply®-Pro 0.5

Biosphere® sterile

neutral 100 pieces 500 pcs./inner case1,000 pcs./case

72.735.002Multiply®-Pro 0.5

---- neutral 500 pieces 2,000 pcs./case

72.735.992 ----mixed colours●●●●●●

500 pieces 3,000 pcs./case

72.737

0.2 ml

Biosphere® Multiply®-Pro 0.2

Biosphere® sterile

neutral 250 pieces 1,000 pcs./inner case2,000 pcs./case

72.737.002

Multiply®-Pro 0.2

---- neutral 500 pieces 2,000 pcs./case

72.737.992 ----mixed colours●●●●●●

500 pieces 3,000 pcs./case

Strips of lid for Multiply®-µStrip and Multiply® PCR plate

Product Order No. Description compatible with Sterile Colour Quality

GradeStrips

per bag Packaging

65.989

strips of 8 lids, flat,

highly transparent, for real-time PCR

Multiply®-µStrip

(72.985.002 /

72.985.992)

and

Multiply®

PCR plate

(72.985)

Biosphere® sterile

neutral

12 strips 240 strips/case

65.989.002 ---- 120 strips 480 strips/case

NEW

NEW

Product Order No. Volume Description Sterile Colour Quality Grade

Pieces per bag Packaging

72.990

0.2 ml

Biosphere® Multiply®-µStripPro

Biosphere® sterile neutral 50 strips

200 strips/inner case400 strips/case

72.990.002

Multiply®-µStripPro

---- neutral 120 strips 480 strips/case

72.990.992 ----mixed colours

●●●●120 strips 480 strips/case

72.991.002 ---- neutral 120 strips 480 strips/case

72.991.992 ----mixed colours

●●●●120 strips 480 strips/case

72.991.103Multiply®-µStrip Pro,

low profile---- neutral 120 strips 1,200 strips/case

72.985.002

Multiply®-µStrip ---- neutral 120 strips 480 strips/case

72.985.992 ----mixed colours

●●●●120 strips 480 strips/case

PCR tube strips with and without lid

NEW

Page 23: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

23

Ordering Information – Multiply® PCR Tubes Accessories: Interchangeable RackSystem for 0.2 ml Multiply® PCR tubes

• Safe and fast handling of Multiply® tubes• Flexible, two-piece system comprising a work tray and support base• Easily inserted in the thermocycler, no need to remove tubes• Convenient 96 well microtiter format to fit any processing system• Stackable design provides space-saving specimen storage

Suitable for all Multiply® tubes

Ordering Information PCR RackSystem

Illustration Order No. Product Description Description Packaging

95.987.002PCR work tray for

0.2 ml PCR tubesfor 96 tubes 5/bag

95.988

Support base for PCR work tray

neutral

5/case

95.988.001 red ●

95.988.002 blue ●

95.988.003 green ●

95.988.004 yellow ●

Assembly 0.2 ml Multiply® tube loading...

...or, alternatively, Multiply® PCR plate loading.

Ice-bath pipetting Specimen centrifugation in completely assembled RackSystem...

...fast and easy place-ment of specimens into the thermocycler.

Position empty PCR work station to rest securely on the support base.

Align single tubes to 45° for easy handling.

The PCR plate comprises 96 tubes in 12 strips of 8 tubes each.

RackSystem can be used as a pipetting station.

Compatible with any microtiter tray rotor.

After amplification, reposition PCR tray onto stackable support base for space-saving storage.

1 2 3 4 5 6

Sample preparation and PCR applications

Flexible, compact work station for sample preparation and space-saving specimen storage

Page 24: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

24

NEW: White PCR plates for quantitative PCR

Cost-effective miniaturisation of qPCR assay in white Multiply® PCR platesFluorescence-based applications like qPCR, particularly those involving small volumes, benefit from the significantly improved reflection characteristics of the white wells. Specimens of low DNA concentration, too, can be more easily detected (see Fig. 1). Compared to transparent wells, a 10-fold fluorescence level can be measured with white wells. Thanks to maximum signal reflection, Ct values are obtained earlier (see Fig. 2). In white plates sealed with qPCR compatible tapes, the reagent volume (SYBR Green) per well can be reduced from 25µl to 15µl without significant changes in sensitivity and stability, enabling a 40% decrease in qPCR reagent costs.

In addition, the white plates minimise adverse effects like “cross talk” that would increase well-to-well variability in transparent wells.

• 10-fold fluorescence level• Improved sensitivity• Enhanced reproducibility

1

AB

CD

EF

GH

23

45

67

89

10 1112

0

5

10

15

20

25

30

35

Ct values for the detection of plasmid copies in white and transparent plates at reduced reaction volumes

Ct v

alue

s

Flu

ore

scen

ce

Number of plasmid copiesFig. 1

Increased sensitivity due to maximum signal reflection

CyclesFig. 2

1,000,000 100,000 10,000 1,000 100

Ct at 15 µl (white)Ct at 25 µl (transparent)

Page 25: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

25

NEW: White PCR plates for quantitative PCR

96 Well Multiply® PCR plate with full skirt, “low profile” well design- Enhanced stability for automated systems- Suitable for real-time systems- Black alphanumeric print for enhanced well identification- Raised well rims for optimal sealing with heat-resistant tape- Can be sealed with qPCR film 95.1999 (see p. 33) or optically clear strips of 8 lids (see p. 26)

72.1980.232

0.2 ml 96 Well Multiply® PCR plate, without skirt- NEW: Easy to cut- Black alphanumeric print for enhanced well identification- Raised well rims for optimal sealing with heat-resistant tape- Fits most commonly used thermocyclers- Can be sealed with qPCR film 95.1999 (see p. 33) or optically clear strips of 8 lids (see p. 26)

72.1978.232

1

AB

CD

EF

GH

23

45

67

89

10 1112

96 Well Multiply® PCR plate with half skirt, flat deck- Recommended for Applied Biosystems (ABI) thermocyclers and DNA sequencers- Black alphanumeric print for enhanced well identification- Flat deck enables safe sealing with tapes- Raised well rims for optimal sealing with heat-resistant tape- Can be sealed with qPCR film 95.1999 (see p. 33) or optically clear strips of 8 lids (see p. 26)

72.1979.132

NEW

Multiply® PCR plates made from polypropylene, white• White wells for improved fluorescence reflection• Certified “PCR Performance Tested” • Thin-walled reaction tubes for quick temperature transfer• Fits thermocyclers processing 96 and 384 well block formats• Barcode labeling on plates with half or full skirt is available on request

Page 26: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

26

NEW: White PCR plates for quantitative PCR1

AB

CD

EF

GH

23

45

67

89

10 1112

White Multiply® PCR plates and lid strips for 96 Well plates

Product Order No. Special feature Description Quality

Grade Colour Packaging

72.1978.232Optimised for

qPCR96 Well,

without skirtwhite

25 pcs./inner case

100 pcs./case

72.1979.132Optimised for

qPCR96 Well, with half skirt

and low profilewhite

25 pcs./inner case

100 pcs./case

72.1980.232Optimised for

qPCR96 Well, with full skirt,

low profile for Fast PCRwhite

25 pcs./inner case

100 pcs./case

72.1981.232*Optimised for

qPCR

ABI style, 96 Well, with half skirt,

low profile for Fast PCRwhite

25 pcs./inner case

100 pcs./case

72.1982.202Optimised for

qPCRLightcycler 480, 96 Well,

with half skirtwhite

25 pcs./inner case

100 pcs./case

72.1985.202Optimised for

qPCRLightcycler 480, 384 Well,

with full skirtwhite

50 pcs./inner case

100 pcs./case

65.1998.002Optimised for

qPCRstrips of 8 lids, flat for 96 Well

plates, highly transparenttransparent 480 pcs./case

* Available on request

NEW

NEW

NEW

72.1985.202

NEW NEW: Lightcycler 480 PCR plate with 384 wells- Max. volume: 30 µl per well- Black alphanumeric print for enhanced well identification- Raised well rims for optimal sealing with heat-resistant tape- Can be sealed with qPCR film 95.1999 (see p. 33)

72.1982.202

NEW NEW: Lightcycler 480 PCR plate with 96 wells- Black alphanumeric print for enhanced well identification- Raised well rims for optimal sealing with heat-resistant tape- Can be sealed with qPCR film 95.1999 (see p. 33) or optically clear strips of 8 lids (see p. 26)

Page 27: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

27

NEW: White PCR plates for quantitative PCR Quality in series – 96 and 384 Well Multiply® PCR plates

Multiply® PCR plates made from polypropylene• Certified 'PCR Performance Tested' • Quick temperature transfer due to thin-walled reaction tubes• Fits thermocyclers processing 96 and 384 well block formats• Raised well rims for improved sealing with heat-resistant tape• Plates with half and full skirt; Barcode available on request

0.2 ml 96 Well Multiply® PCR plate, without skirt- NEW: Easy to cut- Black alphanumeric print for enhanced well identification- Raised well rims for optimal sealing with heat-resistant tape- Fits most commonly used thermocyclers- Can be sealed with optically clear strips of 8 lids (see p. 29)- Can be sealed with qPCR films (see pages 33 – 35)

72.1978.202

96 Well Multiply® PCR plate with half skirt- Recommended for Applied Biosystems (ABI) thermocyclers and DNA sequencers- Black alphanumeric print for enhanced well identification- Suitable for real-time PCR- Can be sealed with optically clear strips of 8 lids (see p. 29)- Can be sealed with qPCR films (see pages 33 – 35)- Also available with barcode label (72.1979.203)

72.1979.202

96 Well Multiply® PCR plate, without skirt, pre-assembled with strips of 8 tubes- Available in the Biosphere® quality, individually wrapped sterile- Suitable for real-time PCR- Can be sealed with optically clear strips of 8 lids (order no. 65.989, see page 29)- Tray from polycarbonate- Suitable for use in RackSystem, see p. 23

72.985

1

AB

CD

EF

GH

23

45

67

89

10 1112

Page 28: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

28

Quality in series – 96 and 384 Well Multiply® PCR plates1

AB

CD

EF

GH

23

45

67

89

10 1112

96 Well Multiply® PCR plate with full skirt, 'low profile' well design- Enhanced stability for automated systems - Black alphanumeric print for enhanced well identification- Suitable for real-time PCR- Can be sealed with optically clear strips of 8 lids (see p. 29)- Can be sealed with qPCR films (see pages 33 – 35)

72.1980.202

96 Well Multiply® PCR plate with half skirt, optimised for ABI Fast PCR System- ‘Low profile’ design reduces dead volume and cycle times- Suitable for real-time systems- Black alphanumeric print for enhanced well identification- Can be sealed with optically clear strips of 8 lids (see p. 29)- Can be sealed with qPCR films (see pages 33 – 35)

72.1981.202

384 Well Multiply® PCR plate with robust skirt- 40 µl maximum volume per well - 8 apertures in the frame facilitate positioning and removal of the plate from the heating block- Black alphanumeric print for enhanced well identification- Can be sealed with qPCR films (see pages 33 – 35)

72.1984.202

NEW: 96 Well Multiply® PCR plate with half skirt, with flat deck- Recommended for Applied Biosystems (ABI) thermocyclers and DNA sequencers- Flat deck for easy and safe sealing of wells with tapes- Black alphanumeric print for enhanced well identification- Suitable for real-time PCR- Can be sealed with optically clear strips of 8 lids (see p. 29)- Can be sealed with qPCR films (see pages 33 – 35)

72.1979.102

NEW

Page 29: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

29

Quality in series – 96 and 384 Well Multiply® PCR plates Ordering Information – Multiply® PCR plates & lid strips

Lid strips for Multiply®-µStrip and Multiply® PCR plate

Product Order No. Description compatible with Sterile Colour Quality

GradeStrips

per bag Packaging

65.989

strips of 8 lids, flat, highly trans-

parent, for real-time PCR

Multiply®

PCR plate

72.985

Biosphere® sterile

neutral

12 strips 240 strips/case

65.1998.002

strips of 8 lids, flat, highly trans-

parent,for real-time PCR

Multiply®

PCR plates

72.1978.202,

72.1979.102,

72.1979.202,

72.1980.202,

72.1981.202

---- 120 strips 480 strips/case

NEW

1

AB

CD

EF

GH

23

45

67

89

10 1112

Multiply® PCR plates, transparent

Product Order No. Well/Max.Volume Description Sterile Colour Quality

Grade Packaging

72.985 96 / 0.3 ml

Biosphere® Multiply® PCR plate

without skirt,pre-assembled,

raised wells

Biosphere® sterile

neutral1 pcs./bag

20 pcs./case

72.1978.20296 / 0.3 ml

Multiply® PCR platewithout

skirt---- neutral

25 pcs./inner case

100 pcs./case

72.1979.102 96 / 0.3 mlMultiply® PCR plate,

with half skirt and flat deck

---- neutral25 pcs./inner case

100 pcs./case

72.1979.202 96 / 0.3 mlMultiply® PCR plate

with half skirt---- neutral

25 pcs./inner case

100 pcs./case

72.1979.20396 / 0.3 ml

withbarcode label

Multiply® PCR platewith half skirt

---- neutral25 pcs./inner case

100 pcs./case

72.1980.202 96 / 0.2 mlMultiply® PCR plate

with skirt 'low profile'---- neutral

25 pcs./inner case

100 pcs./case

72.1981.202 96 / 0.2 mlMultiply®

Fast PCR plate'low profile'

---- neutral25 pcs./inner case

100 pcs./case

72.1984.202 384 / 40 µlMultiply® PCR plate

with skirt ---- neutral50 pcs./bag

50 pcs./case

Page 30: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

30

Multiply® PCR plates - Compatibility Table1

AB

CD

EF

GH

23

45

67

89

10 1112

PCR plate range - Compatibility Table

Model96 Well

without skirt,pre-assembled

96 Well without skirt

96 Wellwith half skirt,

high profile

96 Wellwith full skirt,

low profile

96 Wellwith half skirt,

Fast PCR,low profile

384 Wellwith full skirt

Order No. Standard PCR plates

72.98572.1978.20272.1978.232

72.1979.20272.1979.20372.1979.10272.1979.132

72.1980.20272.1980.232

72.1981.20272.1981.232

72.1984.202

Amersham Bioscience

MegaBace 1000 DNA Analysis System (old Model) ✗ ✗ ✗ ✓ ✗ ✗

MegaBace 1000 DNA Analysis System (old Model) ✗ ✗ ✗ ✓ ✗ ✗

MegaBace 4000 DNA Analysis System ✗ ✗ ✗ ✗ ✗ ✓

MegaBace 5000 DNA Analysis System ✗ ✗ ✗ ✓ ✗ ✓

Applied Biosystems

ABI 2720 ✓ ✓

ABI Prism 7000, 7700 ✓ ✓

ABI Prism 7300, 7500, 7700 real-time PCR-System ✗ ✓

ABI Prism 7900HT real-time PCR-System ✗ ✓ ✓

ABI Prism 7500 Fast real-time PCR-System ✗ ✗ ✓

ABI Prism 7900 Fast real-time PCR-System ✗ ✗ ✓

ABI Prism 7900HT Fast real-time PCR-System ✗ ✗ ✓

ABI Prism 9800 Fast PCR-System ✗ ✗ ✓

Gene Amp 9600 ✓ ✓ ✓

Gene Amp 9700 ✓ ✓ ✓ ✓

Gene Amp 7500/5700 ✓ ✓

PE 2700 ✓ ✓

PE 9600 ✓ ✓ ✓

PE 9700 ✓ ✓ ✓

StepOnePlus Fast real-time PCR-System ✗ ✗ ✓

Seq. ABI Prism 310 Genetic Analyzer ✓ ✓ ✗ ✓*Seq. ABI Prism 3100/3130 Genetic Analyzer ✓ ✓ ✗ ✓*Seq. ABI 3700/3730/3730 XL DNA Analyzers ✓ ✓ ✗ ✓* ✓

Veriti 96 Well / 384 Well ✓ ✓

Veriti Fast 96 Well ✗ ✗ ✗ ✓ ✗

Biometra

T1 ✓ ✓ ✓ ✓

T3 ✓ ✓

T Gradient ✓ ✓ ✓ ✓

TRobot ✓ ✓ ✓

Uno ✓ ✓ ✓

Uno II

NEW

NEW

NEW

NEW

* with compatible adapter from ABI

Page 31: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

31

Multiply® PCR plates - Compatibility Table 1

AB

CD

EF

GH

23

45

67

89

10 1112

Model

96 Well without

skirt,pre-

assembled

96 Well without

skirt

96 Wellwith half

skirt,high profile

96 Wellwith full skirt,

low profile

96 Wellwith half

skirt,Fast PCR,low profile

Lightcycler480,

96 Well, full skirt

384 Wellwith full

skirt

Lightcycler480,

384 Well,full skirt

Order No. Standard PCR plates

72.98572.1978.20272.1978.232

72.1979.20272.1979.20372.1979.10272.1979.132

72.1980.20272.1980.232

72.1981.20272.1981.232

72.1982.202 72.1984.202 72.1985.202

Bio-Rad/MJ Research

CFX 96, real-time ✓iCycler, real-time ✓ ✓ ✗iQ5, real-time ✓ ✓ ✗MyCycler ✓ ✓ ✗Opticon 2 System, real-time / C1000 Serie / S1000 Serie ✗ ✓ ✓PTC-200 ✓ ✓ ✓ ✓ ✓PTC-225/220/221 ✓ ✓ ✓ ✓ ✓PTC-100 ✓ ✓ ✓ ✓

Corbet Research

Palm Cycler ✓

Eppendorf

Mastercycler ep realplex ✓ ✓Mastercycler Gradient ✓ ✓ ✓ ✓Mastercycler pro ✓ ✓ ✓ ✓

Ericom

Deltacycler ✓ ✓ ✗ ✗SingleBlock ✓ ✓ ✗ ✗TwinBlock ✓ ✓ ✗ ✗

MWGA

Primus 96 ✓ ✓ ✓Primus 384 ✓THEQ

The Q-Lifecycler ✓ ✓ ✓

Roche

Lightcycler 480 ✓ ✓

Stratagene

Gradient Temp. Cycler ✓MX3000/4000 Multiplex, QPCR System ✓ ✓Robocycler 96/384 ✓

TechneFlexigene ✓ ✓ ✓Genius ✓ ✓ ✓OMN-E ✓ ✓ ✓ ✓PCR Express ✓ ✓ ✓ ✓ ✓Primus 96 ✓ ✗ ✓Px2/PxE ✓ ✓ ✓ ✓ ✓Quantica, real-time ✓TC412/TC512 ✓ ✓Touchgene Gradient ✓ ✓ ✓ ✓

PCR plate range - Compatibility Table

NEW

Legend: ✓✗

= recommended= not recommended= not checked

Page 32: PCR & Molecular Biology · Multiply® PCR plates White and transparent options in 96/384 well format p. 24 - 31 White PCR plates optimised for qPCR p. 24 - 26 Ordering information

32

96 Deep well MegaBlock® 1.2 / 2.2 ml

MegaBlock® 96 WellThe MegaBlock® 96 Well is ideal for automated processing of sample volumes from 1.2 to 2.2 ml or handling large quantities of backup samples.

• Sealing foils/mats available• Products are manufactured under clean room conditions and protected against contamination• Free from human DNA, DNase/RNase, endotoxins

MegaBlock®

Order No. Description Volume/Material Packaging

82.1970.002 round, raised wells 1.2 ml PS transparent 32 pcs./case

82.1971.002 round, raised wells 1.2 ml PP 32 pcs./case

82.1972.002 square wells 2.2 ml PP 32 pcs./case

MegaBlock® 1.2/2.2 ml, PP

• Each well 100% leak-proof tested • Ideal for backup storage of pharmaceutical substances, DNA isolation, long-term sample storage, and enzyme and tissue culture applications• Solvent-resistant to DMSO• Square cavities(2.2 ml) with round bottom wells with alphanumeric coordination system• Autoclavable*

MegaBlock® 1.2 ml, PS transparent

• Each well 100% leak-proof tested• Ideal for long-term storage of blood samples• Pierceable mat cover• Transparent for viewing• Round bottom wells with alphanumeric coordination system

Lids and tapes for MegaBlock®

Order No. Description Packaging

95.1990.002Cover for 1.2 ml MegaBlock®, pierceable,

suitable for 82.1970.002 and 82.1971.002250 pcs./case;

50 pcs./ inner case; 10 pcs./bag

95.1991.002 Cover for 2.2 ml MegaBlock®, suitable for 82.1972.002250 pcs./case;

50 pcs./ inner case; 10 pcs./bag

95.1995 Aluminium foil, pierceable 100 foils/ inner case

82.1586 Acetate film, transparent 100 foils/ inner case

95.1992 Adhesive tape, 96-fold perforation, pierceable 100 foils/ inner case

95.1993 Strongly adhesive tape, transparent 100 foils/ inner case

Refer to pp. 34 – 35 for a detailed description of 95.1995, 95.1992, 95.1993* Important notes on autoclaving on p. 20

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96 Deep well MegaBlock® 1.2 / 2.2 ml Adhesive sealing tapes for qPCR/PCR, automation and sample storage

Ordering Information

Order No. Description Packaging

95.1999 Highly transparent, suited for qPCR with pressure sensitive adhesive 100 tapes/inner case

Adhesive sealing tapes for qPCRHermetical sealing of micro test plates made from polypropylene, polystyrene and polycarbonate to exclude evaporation and protect samples during applications, storage and transport requires tailor-made tape materials.

The Sarstedt range includes a variety of sealing tapes specially designed to meet the sophisticated requirements of PCR, storage of active substances, and High Throughput Screening. All tapes are produced under clean room conditions to exclude contamination with DNase/RNase and nucleic acids.

Highly transparent adhesive tape for quantitative PCR

The 50 µm polyolefin film is coated with a smear-free, crystal clear adhesive that shows minimal adhesion at room temperature and is, therefore, exceptionally easy to handle. Thanks to the initially low stickiness, the film on the PCR plate can be easily shifted to the correct position, if necessary. The strong adhesive effect is brought about during PCR and as a result of the heat produced by the cover, minimising loss by evaporation.

• Highly transparent film ideally suited for real-time PCR and other fluorescence-based applications

• Excellent sealing properties with minimised evaporation values due to innovative adhesive

• No distortion under thermal strain / prevents cross contamination

• Does not stick to skin or gloves during application

• Compatible with aqueous solutions and organic solvents like DMSO, acetonitrile and methanol

• Functional sealing range: -70°C to 110°C

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Adhesive sealing tapes for qPCR/PCR, automation and sample storage

Ordering Information

Order No. Description Packaging

95.1994 transparent 100 tapes/inner case

Transparent adhesive tape for end point PCR and qPCR

The optically transparent foil is made from a 50 µm polyolefin film with perforated “end tabs” for easy removal.

• Optically transparent foil for standard and real-time PCR

• Removable without leaving any residue and ideally suited for sample storage to -70°C

• Compatible with aqueous solutions and organic solvents like DMSO, acetonitrile and methanol

• Functional sealing temperature range for PCR: up to 110°C

Ordering Information

Order No. Description Packaging

95.1995 aluminium 100 tapes/inner case

Aluminium foil for PCR and sample storage

The heat-resistant, robust, pierceable 38 µm aluminium foil stands out for efficient anti-evaporation protection and resistance to solvents. Perforated “end tabs” can be easily removed after attaching the foil.

• Tape can be pierced with probes and pipette tips

• Removable without leaving any residue

• Ideally suited for storing sample material/compounds to -70°C

• Compatible with aqueous solutions and organic solvents like DMSO, acetonitrile and methanol

• Functional sealing temperature range for PCR: up to 110°C

Ordering Information

Order No. Description Packaing

95.1993 transparent 100 tapes/inner case

Transparent adhesive tape for end point PCR and sample storage, strongly adhesive

The tape is made from a 50 µm polyester film coated with a special adhesive for immediate adhesive effect.

• Efficient anti-evaporation protection due to strongly adhesive effect

• Easily removable due to firm “end tabs”

• Compatible with aqueous solutions and organic solvents

• Functional sealing temperature range: -40° to 110°C

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35

Adhesive sealing tapes for qPCR/PCR, automation and sample storage

Ordering Information

Order No. Description Packaging

95.1992 96 fold perforated, transparent 100 tapes/inner case

Pierceable adhesive tape for DNA sequencers, HPLC and autosamplers

The 50 µm polyester film coated with a strong adhesive has 96 perforated openingsfor easy piercing. The perforated openings are free from adhesive to prevent samples, needles or pipette tips from sticking to the tape.

• Easy sampling with tips or pipetting systems

• Temporary protection against evaporation and ideal for samples in 96 well plates

• Resealable for long-term protection with a second tape (e.g. 95.1993)

• Compatible with aqueous solutions and organic solvents

• Cost-effective tape for use in DNA sequencers, HPLC and autosamplers

• Functional sealing temperature range -40°C to 110°C

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Recommended tape options

Order No. Product description Application Special features Pierceable Material Functional

temperature range

95.199296-fold perforated cover tape

Processing/DNA-sequencer, HPLC, autosampling

Easy to pierce, 96 perforated opening w/o adhesive

yes Polyester -40°C to 110°C

95.1993Transparent PCR tape, strongly adhesive

PCR, sample storageStrongly adhesive, eliminates edges, fringing

no Polyester -40°C to 110°C

95.1994Transparent PCRand qPCR tape

PCR, real-time PCRGood optical transparency, high chemical resistance

no Polyolefin -70°C to 110°C

95.1995Adhesive aluminium foil

Sample storagePierceable light protection for samples, high chemical resistance

yes Aluminium -70°C to 110°C

95.1999Adhesive, optically highly transparentqPCR tape

real-time PCR,fluorescence analyses

Highly transparent, pressure-sensitive adhesive, minimal evaporation rates

no Polyolefin -70°C to 110°C

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SafeSeal micro tubes with push cap

0.5 / 1.5 and 2.0 ml Micro Tubes with sterile Biosphere® purity are routinely used when RNase-, DNA-, ATP-free and non-pyrogenic conditions are essential. Superior to standard reaction tubes, Biosphere® Micro Tubes have been developed and continually enhanced to suit the requirements in medical and molecular-biological research:

Secure SafeSeal cap lockingThe 0.5 / 1.5 and 2.0 ml SafeSeal Micro Tube features a closing mechanism which prevents unintentional popping open under thermal stress.

• Wide hinge for easy and precise closure

• Large writing space on lid

• Centrifugation up to 30,000 x g (2.0 ml Micro Tubes up to 25,000 x g)

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SafeSeal micro tubes with push cap

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Fig.:SafeSeal Micro Tubeindividually wrapped

Minimizing the risk of contaminationTo reduce storage time in open bags and inherent contamination risks, Biosphere® SafeSeal Micro Tubes are packaged 50 pieces per bag. For most stringent demands in sample preparation, individually wrapped SafeSeal tubes in Biosphere® quality are available. Optionally available non-sterile 0.5 / 1.5 and 2.0 ml SafeSeal Micro Tubes in ‘PCR Performance Tested’ quality: DNase-, RNase-, human DNA- and PCR inhibitor-free.

Volume 0.5 ml 1.5 ml 2.0 ml

Description Order No. Packaging Order No. Packaging Order No. Packaging

72.704.400 250/bag

500/inner case 2,000/case

72.706.400 250/bag

1,000/inner case 2,000/case

72.695.400 250/bag

1,000/inner case 2,000/case

sterile · non-pyrogenic · DNA-freeRNase-free · ATP-free

72.704.200 50/bag

250/inner case 500/case

72.706.200 50/bag

250/inner case 500/case

72.695.200 50/bag

250/inner case 500/case

72.704.201individually wrapped 50/inner case

100/case72.706.201

individually wrapped 60/inner case

120/case72.695.201

individually wrapped 60/inner case

120/case

Ordering Information – Micro Tubes with SafeSeal push cap

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UV-Transparent Disposable Cuvettes for photometric analyses in the UV range ≥ 220 nm

The plastic material of the half micro cuvette enables measurements in the UV range from wave lengths of 220 nm. Contamination caused by the repeated rinsing processes required for quartz glass cuvettes can be avoided by using the UV-transparent disposable cuvette. Apart from non-certified UV cuvettes, our range includes a certified option individually sealed with a cap and packed in a Styrofoam container to protect from scratches.

Typical applications and product features • Photometric analysis of nucleic acids at 260 nm and proteins at 280 nm

• Low UV absorbance for DNA, RNA and protein quantification

• Compatible with photometers of 8.5 mm and 15 mm light centre height: Eppendorf Biophotometer, ThermoSpectronic, Perkin Elmer, Bio-Rad, Analytica Jena etc.

• Minimizes absorbance deviations due to consistently accurate optical quality

• Economical low-cost alternative to quartz cuvettes

• Certified UV cuvettes (DNA-/RNase-/Protein-free) individually sealed with caps optionally available for safe sample storage and prevention of contamination

For measurements in the short-wave UV range below 300 nm, the material-related self-absorptive characteristics of PS and acrylic (PMMA) cuvettes increase distinctively as their transmission ration decreases.

nm

Wavelengthin nm

Transmission in %

UV cuvette Acrylic cuvette

Polystyrenecuvette

260 73 23 0

280 86 68 0

313 94 93 66

334 96 97 79

366 97 98 90

405 98 99 95

560 98 99 96

UV cuvette

Acrylic cuvette

Polystyrene cuvette

Transmission values in individual cuvettes

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UV-Transparent Disposable Cuvettes for photometric analyses in the UV range ≥ 220 nm

Technical specificationMin. sample volume: 50 µl

Optical path length: 10 mm

Basic absorbance: ≤ 0.134 A at 260 nm; ≤ 0.065 A at 280 nm

External dimensions (W x D x H): 12.5 x 12.5 x 45 mm

Light centre height (LCH): 8.5 mm and 15 mm

Ordering Information UV Cuvettes

Order No. Length LCH Packaging

67.758 45 8.5 mm 100 pcs. in Styrofoam box; 400 pcs./case

67.758.001 45 8.5 mm100 pcs. ind. sealed with lid, in Styrofoam box; 400 pcs./case

certified DNA-/RNase-/Protein-free

67.759 45 15 mm 100 pcs. in Styrofoam box; 400 pcs./case

67.759.001 45 15 mm100 pcs. ind. sealed with lid, in Styrofoam box; 400 pcs./case

certified DNA-/RNase-/Protein-free

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SARSTEDT AG & Co.P.O. Box 12 20D-51582 NümbrechtPhone +49 2293 3050Fax +49 2293 305- [email protected]

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