Parts of the microscopeParts of the microscope

Pamela GonzalezPamela Gonzalez

99thth grade Biology I grade Biology I

ObjectiveObjective

After completing this tutorial, you should be able to identify and After completing this tutorial, you should be able to identify and describe the parts and functions of the compound microscope with describe the parts and functions of the compound microscope with 90% accuracy.90% accuracy.

TN Biology I state standards 1.0TN Biology I state standards 1.0: The student will investigate the : The student will investigate the structures and functions of cell membranes, organelles, and structures and functions of cell membranes, organelles, and component biomolecules as related to major cell processes by component biomolecules as related to major cell processes by demonstrating appropriate use and care of compound light demonstrating appropriate use and care of compound light microscopes. microscopes.

NETSNETS••S Profile 9-12:S Profile 9-12: 2: Make informed choices among technology 2: Make informed choices among technology systems, resources, and services. (1, 2)systems, resources, and services. (1, 2)

DirectionsDirections

Click on to take you to the previous slide.

Click on to take you to the next slide.

Click on to take you to your microscope menu.

Click on to bring you back to these directions.

This page shows the buttons that you will use to move around in this

tutorial. The main menu provides a labeled picture of a microscope. Each

label on the microscope is a link to more information on that part. Click each

link on the menu before proceeding to the summary. Proceed to the

summary from the microscope menu by clicking the next button. Get started

by clicking the microscope menu button.

Microscope MenuMicroscope Menueyepiece

fine adjustment

coarse adjustment

base

light source

stage

objectivesarm

EyepieceEyepiece The The eyepieceeyepiece is the point is the point

where you look at your where you look at your specimen. specimen.

The The eyepieceeyepiece has a has a magnification of 10X. This is magnification of 10X. This is important to remember when important to remember when calculating the total calculating the total magnification.magnification.

StageStage The The stagestage where your slide is where your slide is

placed for viewing. The placed for viewing. The stagestage can be moved to locate can be moved to locate specimens on the slide. The specimens on the slide. The stage sits above the light stage sits above the light source. source.

When placing a slide on the stage for viewing, remember that to put the stage at its lowest position to avoid accidentally breaking a slide.

ArmArm

The The armarm acts as support acts as support for the microscope and for the microscope and connects the eyepiece connects the eyepiece and base.and base.

The The armarm is one of the is one of the parts you must hold parts you must hold properly secure the properly secure the microscope while carrying microscope while carrying it. it.

Objective lensesObjective lenses

Our microscopes have Our microscopes have four objectivesfour objectives. The . The objectives are 4X, 10X, objectives are 4X, 10X, 40X and 100X. 40X and 100X.

The shortest The shortest objectiveobjective is is

the lowest powerthe lowest power. .

Coarse adjustmentCoarse adjustment

The The course adjustmentcourse adjustment knob is the larger knob knob is the larger knob and is used first when and is used first when bringing a specimen into bringing a specimen into focus. focus.

This moves the stage This moves the stage closer to the objective closer to the objective lens. Use caution to avoid lens. Use caution to avoid breaking the slide when breaking the slide when

doing this.doing this.

Fine adjustmentFine adjustment

The The fine adjustmentfine adjustment knob knob is the smaller knob is the smaller knob protruding from the protruding from the coarse adjustment knob coarse adjustment knob and is used to bring a and is used to bring a clear image into sharper clear image into sharper focus. focus.

The The fine adjustmentfine adjustment knob knob

is used secondis used second..

BaseBase

The The basebase acts as support. acts as support. It is the bottom most part It is the bottom most part of the microscope. of the microscope.

The The basebase, along with the , along with the arm, are used to safely arm, are used to safely carry the microscope to a carry the microscope to a

tabletable. .

Light sourceLight source

Our microscopes use Our microscopes use a a light sourcelight source. .

The The light sourcelight source is a is a 110V light bulb 110V light bulb instead of a mirror. instead of a mirror.

SummarySummary

The The proper way to hold the microscopeproper way to hold the microscope is to is to place one hand under the base and grasp the place one hand under the base and grasp the arm with the other hand. arm with the other hand.

The The objectivesobjectives provide different magnifications provide different magnifications and length tells its strength of magnifications. and length tells its strength of magnifications.

When When calculating the total magnificationcalculating the total magnification of what of what you are viewing, calculate it with the eyepiece you are viewing, calculate it with the eyepiece magnification included.magnification included.

QuizQuiz

When you feel comfortable with the material covered on When you feel comfortable with the material covered on the parts of the microscope and their functions, click the the parts of the microscope and their functions, click the next slide button to take the quiz. next slide button to take the quiz.

Read each question carefully and select the best Read each question carefully and select the best answer. Click on your answer. answer. Click on your answer.

If you answer incorrectly, you can click back to the If you answer incorrectly, you can click back to the question to try again. question to try again.

When you answer correctly, you can click to proceed to When you answer correctly, you can click to proceed to the next question.the next question.

Question 1Question 1

What two parts of the microscope are held What two parts of the microscope are held when properly holding your compound when properly holding your compound microscope?microscope?

A.A. Arm and eyepieceArm and eyepieceB.B. Stage and baseStage and baseC.C. Base and armBase and armD.D. Base and objectivesBase and objectives

Uh-ohUh-oh

Wahoo!Wahoo!

Question 2Question 2

What magnification is the eyepiece?What magnification is the eyepiece?

A.A. 5X5X

B.B. 10X10X

C.C. 15X15X

D.D. 20X20X

Oh-noOh-no

Great job!Great job!

Question 3Question 3

Once a slide is on the stage, which Once a slide is on the stage, which

adjustment focus is used first?adjustment focus is used first?

A.A. Coarse adjustmentCoarse adjustment

B.B. Fine adjustmentFine adjustment

C.C. ObjectiveObjective

D.D. EyepieceEyepiece

Try againTry again

ExcellentExcellent

Question 4Question 4

Before placing a slide on the stage, you Before placing a slide on the stage, you

should do which of the following steps?should do which of the following steps?

A.A. Turn to the highest power objectiveTurn to the highest power objective

B.B. Focus your eyepieceFocus your eyepiece

C.C. Place the stage at its lowest positionPlace the stage at its lowest position

D.D. Place the stage at its highest positionPlace the stage at its highest position

OopsOops

PerfectPerfect

ResourcesResources

Miller, K. R. & Levine, J (2002). Miller, K. R. & Levine, J (2002). BiologyBiology. .

New Jersey: Pearson Education Inc. New Jersey: Pearson Education Inc. All pictures are courtesy of Microsoft All pictures are courtesy of Microsoft

Clipart.Clipart.