Authors: Åsa Sivertsson, Ph.D., and Mathias Uhlén, Ph.D.Bioinformatics: Martin Zahlen, Linn Fagerberg, Ph.D., Max Karlsson, and Kalle von Feilitzen Illustration/Design: Mattias KarlénEditor: Sean Sanders, Ph.D.

Our blood performs an astoundingly broad range of functions, living up to its role as an essential connective tissue in the body. It is a transportation provider, a “trash collector,” and a sentinel protecting against disease. Blood is the highway that moves oxygen from the lungs to tissues throughout the body and, just as importantly, also carries carbon dioxide—the waste product of respiration—on the return journey back to the lungs to be exhaled. It carries nutrition absorbed by the gut in the form of sugars, fats, carbohydrates, and other essential molecules and ions, via the liver, to the most distant extremities. Blood is also the communication system by which hormones, cytokines, and other molecules can transmit information from one side of our body to the other, enabling the whole system to function smoothly and efficiently.

However, blood is equally good at carrying detrimental agents, including infections and metastasizing cancer cells. To fight these nefarious players, the body calls upon the immune system to remove them. It tirelessly surveils the blood system as well as other parts of the body, identifying and removing the unwanted intruders.Although we have made significant advances in our understanding of the immune system in recent decades, we still have much to learn, particularly about the similarities and differences between different immune cells with respect to genomics, proteomics, and transcriptomics.

The Human Blood Atlas (www.proteinatlas.org/blood),described on the front of this poster, furnishes detailedinformation on the expression levels of all humanprotein–encoding genes found in major blood immune-cellpopulations. Having this information enables researchers to better understand not only the normal function of immune cells, but also how they respond to disease—which may offer clues that can lead to improved treatments and novel therapies.

Cells of the immune system also secrete proteins into the bloodstream, most importantly, antibodies, to join the multitude of other molecules found there, including hormones, cytokines, growth factors, enzymes, and clotting factors. The Human Secretome subsection of the Human Protein Atlas (www.proteinatlas.org/blood/secretome) describes these blood-borne components, providing insight into the blood’s chemical makeup and how it is impacted by disease.

We trust that you will find the information provided here a helpful introduction to the Human Blood Atlas, and that this unique database will prove to be a valuable resource for your research.

Roger Goncalves, Sales ManagerCustom PublishingEurope, Middle East, and Indiargoncalves@science-int.co.uk+41-43-243-1358

Sean Sanders, Ph.D.Senior Editor, Custom Publishing, Science/AAAS

Produced by the Science/AAAS Custom Publishing OfficeSponsored byCreated by The Human Protein Atlas.Supported by 1000`s of publications.

Validated by enhanced validation. Evidenced by advancing science.

VERY RELIABLE ANTIBODIES

AtlasPoster Ad 194x260 Final version 24 maj.indd 2 2019-05-24 18:01

VERY RELIABLE ANTIBODIESBuilding on our heritage with the Human Protein Atlas project, we provide highly validated reagents that enable leading research in biology, diagnostics, and medicine for the purpose of understanding and improving human health. We are the original manufacturer of over 21,000 primary antibodies targeting the majority of human proteins. Our Triple A Polyclonals and PrecisA Monoclonals antibodies are available worldwide.

CREATED BY THE HUMAN PROTEIN ATLASTriple A Polyclonals are developed within the prestigious Human Protein Atlas project. Our antibodies are validated in all major human tissues and organs and in 20 cancer tissues. Each antibody is supported by over 500 staining images.

VALIDATED BY ENHANCED VALIDATION We take great care to validate our antibodies in IHC, WB, and ICC-IF. As an additional layer of security, we perform Enhanced Validation. By using 5 different methods, we gain the power to validate each combination of protein, sample, and application.

EVIDENCED BY ADVANCING SCIENCEOur antibodies are used all over the world and referenced in 1000s of scientific peer-reviewed papers.

ATLAS ANTIBODIES AND THE HUMAN PROTEIN ATLAS: OUR JOURNEY TOGETHERThe Human Protein Atlas (HPA) is founded: a unique world-leading effort to create a complete IHC-based map of the human proteome.

The first version of the HPA website is launched. It consists of protein expression data based on approximately 700 antibodies.

Atlas Antibodies is founded by HPA researchers to make the Triple A Polyclonals antibodies developed by the HPA project available for researchers worldwide.

Atlas Antibodies starts the in-house development of PrecisA Monoclonals: mouse monoclonal antibodiesagainst a selected number of targets.

HPA releases the Tissue Atlas: a complete map of protein expression in all major organs and tissues in the human body.

HPA releases the Pathology Atlas: prognostic association of all protein-coding genes in 17 human cancer types including >900,000 Kaplan–Meier plots.

Atlas Antibodies launches QPrEST: high-quality, stable, isotope-labeled internal standards for MS-based protein quantification.

HPA releases the Brain Atlas: protein expression mapping in the human, pig, and mouse brains, together with transcriptomics data and protein profiling down to single-cell detail.

HPA releases the Blood Atlas: single-cell, genome-wide RNA expression profiles of human protein-coding genes covering B-cells, T-cells, monocytes, granulocytes, and dendritic cells.

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GENETIC VALIDATION Specificity confirmed by genetic silencing

INDEPENDENT ANTIBODY VALIDATION Target confirmed by an antibody targeting a different epitope

ORTHOGONAL VALIDATION Target confirmed by a non-antibody based method

RECOMBINANT EXPRESSION VALIDATION Target confirmed by an overexpressed or tagged version of the protein

MIGRATION CAPTURE MS VALIDATION Presence of target verified by mass spectrometry (MS)

STABLE ISOTOPE-LABELED PROTEIN STANDARD FORABSOLUTE QUANTIFICATION USING MASS SPECTROMETRY

QPrESTs are heavy-labeled protein fragments designed to cover the most unique sequences of each protein in the human proteome.

They are used as internal standards that are added early on in the workflow and show a cleaving efficiency similar to the endogenous proteins, thus minimizing error and increasing accuracy in the quantification.

The accuracy of the standard is then further validated by the possibility of looking at ratios of multiple intrinsic peptides.

atlasantibodies.com

HPA releases the Cell Atlas: high-resolution insights into the spatiotemporal distribution of 12,000 genes in 64 human cell lines with ICC-IF antibody-based profiling.

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