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© 2008 FoodReg AG – All rights reserved © 2007 FoodReg – All rights reserved Confidential Other Origin Determination Methods CHAI CHEUN KUAN Selamat Traceability Workshop 12 & 13 May 2008

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Page 1: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved© 2007 FoodReg – All rights reserved Confidential

Other Origin Determination Methods

CHAI CHEUN KUANSelamat Traceability Workshop

12 & 13 May 2008

Page 2: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Contents1. Functional roles of Traceability

2. IT in traceability system

3. Fundamentals of Traceability

4. Other methods of Traceability

• Introduction

• Principle

• Applications

Page 3: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Functional Roles of Traceability

1. Food safety incident

2. Food residue surveillance programme

3. Risk Assessment from food exposure

4. Enforcement of labelling claims

5. Fraud

6. Food wastage

7. Hygiene Identified by: UK Food AgencyIdentified by: UK Food Agency

Page 4: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

IT in Advance Traceability System

Database

Page 5: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Traceability System ‐ Fundamentals

1. Unique identification of a product (or batch)

2. Collection  of  accurate  information  (records) throughout its production processes

3. Information  management  and  data recoverability 

Page 6: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Unique Identification

Page 7: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

External Identifier

GS1)

RFID Label

RG CodeID Tag

Laser Surface Scanning

Page 8: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Laser Surface Scanning

1. Introduction

2. General Principle

3. Applications

Page 9: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Introduction – Laser surface Scanning

a) Presence  of  individual  “fingerprint” of  an object in nature

b) Laser scanning takes place on the designated         surface of an object and record the naturally random  microscopic  surface  structure  / pattern

“Fingerprint” of an object = microscopic pattern

Page 10: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

a) Verify wide range of materials such as paper, plastic, metal and even ceramic etc

b) Common material used in food packaging, documents or ID tags

Introduction – Laser surface Scanning

Page 11: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

General Principle

Pattern registrationSearch and compare

Yes. Original object

Object surface(Packaging)

Microscopic Structure

Page 12: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Applications

a) Packaging

b) ID Tags

c) Certificates

d) Documents (Delivery notes & Invoices)

Page 13: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Biometric Identifier

Retinal Scanning Nose Printing

DNA Profiling

Page 14: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

DNA Method

1. Introduction

2. General Principle

3. Applications

Page 15: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Introduction – DNA Method

Francis Crick James Watson

Page 16: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Introduction ‐ DNAa) Deoxyribonucleic acid = DNA

b) Database of living organism and some viruses which contains genetic information

c) It is a double helix complementary structure molecule

Page 17: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Introduction ‐ Basic Unit of DNA

Page 18: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Introduction ‐ Double Helix Structure

Page 19: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

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General Principle

a) DNA Extraction

b) Polymerase Chain Reaction (DNA 

Marker)

c) Gel Electrophoresis

Page 20: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Polymerase Chain Reaction (PCR)a) A molecular biology technique. 

Polymerase is an enzyme which perform replication and transcription works

b) DNA present in small amount. This technique is used to produce (amplify) many copies of DNA

c) PCR is a repeating process of:(Denature ‐> Anneal ‐> extension)n

Page 21: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Polymerase Chain Reaction (PCR)

Enzyme (Polymerase) DNA marker PCR in progress

Page 22: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Gel Electrophoresisa) A technique to separate the DNA, 

ribonucleic acid (RNA), or protein molecules by using an electric current applied to a gel matrix

b) DNA is negative‐charged due to the sugar‐phosphate backbone. So it migrates from negative to positive electrodes through the gel matrix. 

Page 23: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Gel Electrophoresisc) Therefore a larger molecules migrate 

slower than small molecules

Page 24: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Application 1

Search and compare

DNA profile storing

Backward traceSample

Page 25: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Availability of DNA Marker

a) Catfish

b) Penaeid shrimps

c) Common carp

d) Salmon

e) Trout…… etc

Page 26: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Application 2

Page 27: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

© 2008 FoodReg AG – All rights reserved

total traceability

Summary

a)Progressive development of new method to 

improve traceability and authenticity

b)Information technology plays an important role in 

data storage and management 

Page 28: Origin Determination Via Other Methods · 1. Food safety incident 2. Food residue surveillance programme 3. Risk Assessment from food exposure 4. Enforcement of labelling claims 5

total traceability

© 2008 FoodReg AG – All rights reserved

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