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Other Origin Determination Methods
CHAI CHEUN KUANSelamat Traceability Workshop
12 & 13 May 2008
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total traceability
Contents1. Functional roles of Traceability
2. IT in traceability system
3. Fundamentals of Traceability
4. Other methods of Traceability
• Introduction
• Principle
• Applications
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total traceability
Functional Roles of Traceability
1. Food safety incident
2. Food residue surveillance programme
3. Risk Assessment from food exposure
4. Enforcement of labelling claims
5. Fraud
6. Food wastage
7. Hygiene Identified by: UK Food AgencyIdentified by: UK Food Agency
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total traceability
IT in Advance Traceability System
Database
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total traceability
Traceability System ‐ Fundamentals
1. Unique identification of a product (or batch)
2. Collection of accurate information (records) throughout its production processes
3. Information management and data recoverability
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total traceability
Unique Identification
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total traceability
External Identifier
GS1)
RFID Label
RG CodeID Tag
Laser Surface Scanning
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total traceability
Laser Surface Scanning
1. Introduction
2. General Principle
3. Applications
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total traceability
Introduction – Laser surface Scanning
a) Presence of individual “fingerprint” of an object in nature
b) Laser scanning takes place on the designated surface of an object and record the naturally random microscopic surface structure / pattern
“Fingerprint” of an object = microscopic pattern
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total traceability
a) Verify wide range of materials such as paper, plastic, metal and even ceramic etc
b) Common material used in food packaging, documents or ID tags
Introduction – Laser surface Scanning
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total traceability
General Principle
Pattern registrationSearch and compare
Yes. Original object
Object surface(Packaging)
Microscopic Structure
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Applications
a) Packaging
b) ID Tags
c) Certificates
d) Documents (Delivery notes & Invoices)
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Biometric Identifier
Retinal Scanning Nose Printing
DNA Profiling
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total traceability
DNA Method
1. Introduction
2. General Principle
3. Applications
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total traceability
Introduction – DNA Method
Francis Crick James Watson
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total traceability
Introduction ‐ DNAa) Deoxyribonucleic acid = DNA
b) Database of living organism and some viruses which contains genetic information
c) It is a double helix complementary structure molecule
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Introduction ‐ Basic Unit of DNA
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Introduction ‐ Double Helix Structure
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General Principle
a) DNA Extraction
b) Polymerase Chain Reaction (DNA
Marker)
c) Gel Electrophoresis
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Polymerase Chain Reaction (PCR)a) A molecular biology technique.
Polymerase is an enzyme which perform replication and transcription works
b) DNA present in small amount. This technique is used to produce (amplify) many copies of DNA
c) PCR is a repeating process of:(Denature ‐> Anneal ‐> extension)n
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Polymerase Chain Reaction (PCR)
Enzyme (Polymerase) DNA marker PCR in progress
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total traceability
Gel Electrophoresisa) A technique to separate the DNA,
ribonucleic acid (RNA), or protein molecules by using an electric current applied to a gel matrix
b) DNA is negative‐charged due to the sugar‐phosphate backbone. So it migrates from negative to positive electrodes through the gel matrix.
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total traceability
Gel Electrophoresisc) Therefore a larger molecules migrate
slower than small molecules
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total traceability
Application 1
Search and compare
DNA profile storing
Backward traceSample
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total traceability
Availability of DNA Marker
a) Catfish
b) Penaeid shrimps
c) Common carp
d) Salmon
e) Trout…… etc
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Application 2
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total traceability
Summary
a)Progressive development of new method to
improve traceability and authenticity
b)Information technology plays an important role in
data storage and management
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total traceability
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