Nutritional requirement Growth Metabolism Serum Topoinhibition Source substitute pH requlation

presented by : ANMOL FATIMA

Nutrients  components in foods that a cell uses to survive and grow are known as nutrients

NUTRITIONAL REQUIREMENT For mammalian cell and plant cell culturing

Amino acids (arginine, leucine etc) Vitamins ( riboflavin, biotin, vitamin

B12) Inorganic ion ( sodium ,potassium, calcium) Trace elements (molybdenum, copper) Monosaccharide ( sugar ,glucose, galactose )

NUTRITIONAL REQUIREMENT Hormone in case of plant cell culturing

auxin ,cytokin , gibberellin, absic acid are required

in case of mammalian cell culturing somatotrophin,

hydrocortison ,prolectin etc are required

NUTRITIONAL REQUIREMENT In case of plant cell culturing

Undefined organic supplement required

For example protein hydrosylate Coconut milk Yeast charcoal

GROWTH

Growth refers to a positive change in size, and/or maturation, often over a period of

time.

GROWTH

GROWTH

 Characteristic Growth Pattern of Cultured Cells.

  Lag phase The first phase of growth after the culture is

seeded is the lag phase, which is a period of slow growth when the cells are adapting to the culture environment and preparing for fast growth.

 

Exponential phase  The lag phase is followed by the log phase

(i.e., “logarithmic” phase) a period where the cells proliferate exponentially and consume the nutrients in the growth medium. 

GROWTH Stationary phase

When all the growth medium is spent (i.e., one or more of the nutrients is depleted) or when the cells occupy all of the available substrate, the cells enter the stationary phase where the proliferation is greatly reduced or ceases entirely

CELL METABOLISM Metabolism is sum total of all chemical

process Take place in living cell

Catabolism -breaking down Anabolism –building up

SERUM

the component of blood that is neither a blood cell (serum does not contain RBC or

WBCs) nor a clotting factor it is the blood plasma not including the fibrinogens.

SERUM

SERUM =PLASMA-CLOTTING FACTOR

SERUM

Serum includes all proteins except blood clotting(coagulation)andelectrolyte, antibodies, antigens, hormones, and any exogenous substances (e.g., drugs and microorganisms).

SERUM

Serum can derive from different animal like Horese serum and human serum

Specific serum use for growth of specific cell type

TYPE OF SERUM

Foetal bovine serum(also known as foetal calf serum )

Newborn calf serum Donor calf serum Horse serum Chicken serum Human serum

TOPOINHIBITION

Inhibition of cells surface proliferation as the cell become closely packed on a culture dish they generally suppressed by the term density dependent inhibition is called topoinhibition

The cell would able to receive and transmit signal to the nucleus both positive and negative signal

TOPOINHIBITION

In tissue culture positive signal are provided by substrate (glass or plastic)

Negative signal provided by intercellular contacts topo inhibition

TOPOINHIBITION Serum may reduce topoinhibition by

shielding its receptors Migration of wound would reduce

topoinhibition By reducing intercellular contacts

PH REQULATION Most normal mammalian cell lines grow well at

pH 7.4, and there is very little variability among different cell strains.

However, some transformed cell lines have been shown to grow better at slightly more acidic environments (pH 7.0–7.4), and

some normal fibroblast cell lines prefer slightly more basic environments (pH 7.4–7.7)

Insect cell lines such as Sf9 and Sf21 grow optimally at pH 6.2.

SOURCE SUBSTITUTE  thing that takes the place of someone or

something else are called substitute

Iron salt as a replacement for transferrin

Transferin is used in culture as an iron transporter for animal cells however for protein free medium that do not contain component of animal origin ,transferin can be replace by ferric salt