numira core-presentation-012414
TRANSCRIPT
Kim Killian
Regional Manager So Cal
February 21, 2014
Oncology
Services
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About Us
SPECIALTY CRO that combines novel tools & technologies to provide unique solutions to a wide variety of preclinical drug discovery efforts
RECOGNIZED LEADER for high-resolution 3D imaging and analysis, specialized histology, and
3D cell screening oncology services
High-quality portfolio of services for broad range of Rx areas, including: PULMONARY,
ONCOLOGY, INFLAMMATION, BONE, DEVELOPMENTAL BIOLOGY, DEVICES
Helping the Life Sciences community develop better products through
the most innovative tools and technologies
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MicroCT Imaging and Analysis
RA Rat Paw
AltaBlu-perfused Rat Brain
Surface Roughness
Fibrotic Mouse Lungs
Vessel Radius
Segmented Fibrotic Tissue
UNIQUE
Image Analysis and Quantification
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Pulmonary ServicesBleomycin-induced Lung Fibrosis
The Problem
The fibrotic lesions generated by bleomycin are
heterogeneous and vary depending on the route of
induction.
The heterogeneous nature of the lesion makes it
challenging at best to reconcile hydroxyproline
measures with the histological measures, particularly
with the intra-tracheal administration model.
Our unique solution
Using a patented contrast agent and high-resolution
microCT imaging, we obtain images that allow you to
visualize all of the fibrosis in the entire lung at a
glance.
The fibrosis is quantified and graphed for
statistical analysis for the entire lung.
The imaging is non-destructive. The
comprehensive nature of imaging data allows a
focused approach to histology sampling or
hydroxyproline measures of collagen.
Control (normal) and Fibrotic (bleomycin-induced)
lungs showing fibrotic lesions
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Pulmonary ServicesBleomycin-induced Lung Fibrosis – MicroCT Results
3 normal and 3 (bleomycin-induced) fibrotic lungs scanned at 10μm resolution using MicroCT
Subcutaneous implantation of micro-pump calibrated to deliver measured dosages with an
exposure duration of 7 days
Pump removed and specimens allowed to ambulate freely for 5 weeks after administration of
reagent
Specimen lungs were perfused to facilitate blood clearance
Sample No.
Hyper-attenuating
Tissue Volume
(mm3)
Total Tissue
Volume (mm3)
Ratio
(HTV/TTV)
52 (Normal) 1.682 242.493 0.69%
153 (Normal) 2.292 220.171 1.04%
154 (Normal) 3.927 210.557 1.87%
353 (Fibrotic) 9.708 187.963 5.16%
354 (Fibrotic) 36.878 212.730 17.34%
454 (Fibrotic) 13.328 219.508 6.07%
Fibrotic
Normal
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Pulmonary ServicesBleomycin-induced Lung Fibrosis
Hyper-attenuating tissue
highlighted in yellowTraditional Histology MicroCT Imaging Data
Comparison between traditional histology and microCT data for a single coronal section
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Pulmonary Services
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Fibrosis InflammationNormal
Comparison – Lungs with fibrotic, normal, and inflammatory tissue
Emphysema / COPD
MicroCT Imaging and
3D Analysis
Pulmonary Arterial
Hypertension
Vascular analysis
using AltaBluTM
Analysis using
Immunohistochemistry
(IHC)
Additional Services
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Vasculature Services
Kidney
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AltaBluTM – Vascular Casting Agent
Comprehensive visualization of vasculature features (even within bone)
Can be used with Histology & IHC
Easy to use; no mixing; comes pre-formulated
Easy to clean up (with water)
More successful perfusions
More consistently penetrate capillaries8
AltaBlu PrimeTM – Vascular Casting Agent
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Histology ServicesFull Service GLP Histology Suite
Routine Histology
Paraffin, Frozen, and Plastic
Specialized Histology
Thin sections and EXAKT ground sections for hard
biomaterials including metals
Oversized sections
Specialized expertise in ocular and brain
Histomorphometry
GLP-validated Osteomeasure systems
Static, dynamic, and cellular measurements
Histopathology
Bone, Oncology, CNS, Pulmonary, Gastrointestinal, and
Renal
Special Stains
Several stains including Goldner’s, TRAP, Masson’s, and
Silver Stain
Immunohistochemistry
Method development
Co-expression and double labeling
Image Analysis
Custom analysis tools and segmentation available
Comparison between traditional histology and microCT data for a single coronal section.
Post soft tissue imaging, samples can further be used for IHC or traditional histology.
Traditional Histology MicroCT Imaging Data
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Histology ServicesImage Analysis
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Custom segmentation and image analysis
using in-house software tools (VHLab)
Color-based analysis for different features of
interest
Area/Size calculations for specific stains or
features
Stain color distribution
Cell count
Study-dependent custom metrics
No. of glomeruli analyzed: 6
Glomerular tuft area (blue regions): 57051 sq. microns
Brown stained area (red regions): 28297 sq. microns
Average radius: 43.57 ± 10.76 microns
Glepp-1 stained Normal Rat Kidney
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Software ServicesAltaNexusTM – Cloud-based Service
https://AltaNexus.com
Security: authenticated logins, encrypted storage, and secure web-connections
Data access: searchable, sortable data organized by project and specimen
High speed: instant access to every aspect of your data
No limits: unlimited data storage, unlimited upload and download bandwidth
Document previews: view Office documents in the browser
Media streaming: play content directly in your browser
∞
Numira’s cloud-based service provides a secure web interface for customers to manage projects and explore their
datasets, media, and documents. Features of AltaNexusTM include: