nucleic acid electrophoresis -...

NUCLEIC ACIDELECTROPHORESIS

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Horizontal ElectrophoresisSelection Guide

Product Page # Tray Size Maximum Cooling External Built-InName (w x l cm) Sample Capacity Option Casting Power SupplyHE33 36 7 x 10 32 Yes Yes –HE99X 38 15 x 10 30 – Yes –

15 x 15 6015 x 20 60

SUB6 40 6 x 7.5 32 – Optional –SUB10 42 10 x 11.5 80 – Optional –SUB13 44 12.8 x 15 112 – Optional –SUB15 46 15 x 15 120 – Yes –SUB20 48 20 x 20 160 – Yes –SUB20C 48 20 x 20 160 Yes Yes –SUB25 50 25 x 30 624 – Yes –SUB25C 50 25 x 30 624 Yes Yes –SUBHT 52 15 x 15 120 – Yes –HE-PLUS 54 12.5 x 13 112 – Yes Yes

12.5 x 6 566 x 6 8

The recommended power supply for the above non-powered horizontal units is the Hoefer PS300B (see page 70).

Hoefer, the premier manufacturer of electrophoresis equipment, has expandedits family of horizontal electrophoresis units providing a solution for almostevery nucleic acid application.

Hoefer’s HE33 and HE99X classic horizontals have been used in electrophoresislaboratories around the world for more than 10 years.

Hoefer is pleased to introduce a new line of submarine units which offers eightmodels from mini units to large cooled units in a new contemporary design.

The Hoefer line has been further expanded to include a high throughputhorizontal as well as a horizontal unit with a built-in variable power supply.

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HE33 Mini HorizontalAgarose Electrophoresis UnitPerform rapid separation of DNA fragments

The HE33 Mini Horizontal Agarose Unit is designed for very fastseparations of DNA restriction fragments in agarose gels. To achievepassive cooling, you simply fill the clear molded base with 50%ethylene glycol coolant solution, seal it, and place it in a freezer or

ice bath until ready to use.

Technical SpecificationsGel Dimensions (w x l) . . . . . . . . 7 x 10 cmMaximum Buffer Volume . . . . . . 250 mlMaximum Power Settings . . . . . . 500 V for (5 min or less), 500 mA, 15 WMaximum Sample Capacity . . . . 32Maximum Temperature . . . . . . . 50°CIndoor Use . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (l x h x w) . . . . 24 x 7 x 13 cmSafety Certifications . . . . . . . . . . EN61010-1, UL61010A-1, CSA C22.2 1010.1, CE

Ordering InformationCat. # DescriptionHE33-8-1.5 HE33 Mini Horizontal Unit, Complete

Cat. # DescriptionHE33B HE33 Mini Horizontal Unit, Basic

Complete Unit Includes:• Buffer Chamber Assembly • Foam Gaskets–4 pcs• Safety Lid w/High Voltage Leads • Bubble Level• Running Tray • 1.5 mm thick, 8 well comb• Casting Tray • Comb Back

Order HE31A combs and HE31-BK comb backs separately, see page 37.

Basic Unit Includes:• Buffer Chamber Assembly • Casting Tray• Safety Lid w/High Voltage Leads • Foam Gaskets–4 pcs• Running Tray • Bubble Level

Features and BenefitsFast separations–run a 7 x 10 cm gelin as little as 5 minutes

UV transparent running tray–allowsthe user to image the gel withoutrisk of damage due to handling

Variety of comb sizes–enablescustomization of samplethroughput to fit your laboratoriesrequirements

Adjustable comb height–completecontrol over well depth

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HE33 CombsCat. # # of Wells Thickness Well Width Sample Volume in a

(mm) (mm) 5 mm Deep Well (µl)HE31A-P-1.0 1/2* 1.0 44/6 235/35HE31A-P-1.5 1/2* 1.5 44/6 355/50HE31A-8-1.0 8 1.0 6.5 32.5HE31A-8-1.5 8 1.5 6.5 48.5HE31A-12-1.0 12 1.0 3.9 19.5HE31A-12-1.5 12 1.5 3.9 30.5HE31A-16-1.0 16 1.0 2.6 13HE31A-16-1.5 16 1.5 2.6 20.5

HE33 Mini HorizontalAccessories and Replacement Parts

Accessories and Replacement PartsCat. # DescriptionHE47-10 Gel Casting Kit:

7 x 10 cm Running Tray7 x 10 cm Casting TrayFoam Gaskets–4 pcs

HE42-10 7 x 10 cm Running TrayHE45-10 7 x 10 cm Casting TraySER11 Bubble LevelHE48 Foam Gaskets–4 pcsHE38TP Top Fill Plug Kit–4 pcsHE31-BK Comb Back + 2 screwsHE30 Buffer Chamber AssemblyHE36 Lid w/High Voltage LeadsSE6056-HV High Voltage Leads–2 pcsHE39 Electrode Replacement Kit

HE47-10

HE42-10

HE45-10

HE38TP

SER11

*1 preparatory and 2 reference wellsAll combs require HE31-BK comb back ordered separately

HE36HE30 HE39

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HE99X Max HorizontalAgarose Electrophoresis Unit

Offers a variety of gel lengths and comb sizesto enable the design of the gel required foroptimum resolution of DNA fragments

The HE99X Max agarose electrophoresis unit facilitates highresolution separation of nucleic acid fragments 100 bp to 20 kbp.Three different length trays are available for use with this unit.

Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . . . . . 15 x 20 cm, 15 x 15 cm, or 15 x 10 cmMaximum Buffer Volume . . . . . . . . . . . . . . 1.2 LMaximum Power Settings . . . . . . . . . . . . . . 200 V, 100 mA, 20 WMaximum Sample Capacity . . . . . . . . . . . . 60Maximum Temperature . . . . . . . . . . . . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (l x h x w) . . . . . . . . . . . . 36 x 14 x 18.2 cmSafety Certifications . . . . . . . . . . . . . . . . . . EN61010-1, UL61010A-1,

CSA C22.2 1010.1, CE

Ordering InformationCat. # DescriptionHE99X-15-1.5 HE99X Max Horizontal Unit, Complete

Complete Unit Includes:• Buffer Chamber Assembly • Foam Gaskets–4 pcs• Safety Lid w/High Voltage Leads • Bubble Level• Running Tray, 15 x 20 cm • 1.5 mm thick, 15 well comb• Casting Tray, 15 x 20 cm • Comb Back

Cat. # DescriptionHE99X HE99X Max Horizontal Unit, Basic

Basic Unit Includes:• Buffer Chamber Assembly• Safety Lid w/High Voltage Leads• Bubble Level Order a casting kit, HE91A combs and HE91-BK comb backs separately, see page 39.

Features and BenefitsSelf-locating/centering guides–supports consistent running trayplacement

Gel anchor feet–prevent the gelfrom floating or sliding duringthe run

Variety of comb sizes–enablescustomization of samplethroughput to fit your laboratoriesrequirements

Adjustable comb height–completecontrol over well depth

Three casting kits available–enabling users to run 15 x 20,15 x 15, or 15 x 10 cm gels in thesame unit

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HE99X CombsCat. # # of Wells Thickness Well Width Sample Volume in a

(mm) (mm) 5 mm Deep Well (µl)HE91A-P-1.5 1/2* 1.5 113/10 855/72.5HE91A-P-3.0 1/2* 3.0 113/10 1710/145.5HE91A-10-1.5 10 1.5 9.7 72.5HE91A-10-3.0 10 3.0 9.7 145.5HE91A-15-1.0 15 1.0 7.1 35.5HE91A-15-1.5 15 1.5 7.1 53.5HE91A-15-3.0 15 3.0 7.1 106.5HE91A-20-1.0 20 1.0 4.7 23.5HE91A-20-1.5 20 1.5 4.7 35.5HE91A-20-3.0 20 3.0 4.7 70.5HE91A-30-1.0 30 1.0 3.0 15

Accessories and Replacement PartsCat. # DescriptionGel Casting Kit (Running Tray, Casting Tray, Foam Gaskets–4 pcs)HE97X-20 15 x 20 cmHE97X-15 15 x 15 cm HE97X-10 15 x 10 cmRunning TrayHE92X-20 15 x 20 cmHE92X-15 15 x 15 cmHE92X-10 15 x 10 cmCasting Tray (with Foam Gaskets–4 pcs)HE95X-20 15 x 20 cmHE95X-15 15 x 15 cmHE95X-10 15 x 10 cmHE91-BK Comb Back + 2 screwsSER11 Bubble LevelHE98X Foam Gaskets–4 pcsHE90X Buffer Chamber AssemblyHE96X Lid w/High Voltage LeadsSE6056-HV High Voltage Leads–2 pcsHE99XRK-1 Electrode Replacement Kit

Running Tray

Casting Tray

Gel Casting Kit

HE90X

SER11

HE91-BK

HE96X

HE99X Max HorizontalAccessories and Replacement Parts

*1 preparatory and 2 reference wellsAll combs require HE91-BK comb back ordered separately

HE99XRK-1

HE98X

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Features and BenefitsColor-coded and height adjustablecombs–easily identify combthickness at a glance and controlwell depth

Colored loading strips–for easy welldetection when loading

Compact tank–reduces the buffervolume required to cover the gel,providing greater control over thevoltage gradient and run-time

UV-transparent running tray–allows the user to image the gelwithout risk of damage due to handling

Side handles–for safe and easytransportation around thelaboratory

SUB6 Mini Submarine Gel Electrophoresis UnitIdeal for routine preparatory and analytical electrophoresis techniques

Ordering InformationCat. # DescriptionSUB6 Mini Submarine Gel Unit

Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . 6 x 7.5 cmMaximum Buffer Volume . . . . . . . . . . 325 mlMaximum Power Settings . . . . . . . . . 300 V, 200 mA, 60 WMaximum Sample Capacity . . . . . . . . 32Maximum Running Temperature . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . . 13 x 24 x 6.5 cmSafety Certifications . . . . . . . . . . . . . . EN61010-1, CE

Includes:• Buffer Chamber Assembly • Running Tray w/Silicone Gasket• Safety Lid • 1 mm thick, 8 well combs–2 pcs• High Voltage Leads • Colored Loading Strips

The SUB6 Mini Horizontal Gel Unit has a maximum32-sample throughput capacity and features aremovable gel-casting tray with end gaskets that allow

gels to be cast directly within the tank.

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SUB6 In-Tank CastingThe included running tray is lined withsilicone gaskets which form a leak-proof seal against the inner walls of therunning chamber when the running trayis turned 90° to the direction ofelectrophoresis.

SUB6 CombsCat. # # of Wells Thickness Well Width Volume in 5 mm

(mm) (mm) Deep Well (µl)SUB6-C1-8 8 1 4.5 20SUB6-C1.5-8 8 1.5 4.5 30SUB6-C2-8 8 2 4.5 40 SUB6-C1-12MC 12* 1 2.5 11SUB6-C1.5-12MC 12* 1.5 2.5 17SUB6-C2-12MC 12* 2 2.5 22SUB6-C1-16 16 1 2.2 10SUB6-C1.5-16 16 1.5 2.2 15SUB6-C2-16 16 2 2.2 20

Accessories and Replacement Parts Cat. # DescriptionSUB6-TANK Buffer Chamber AssemblySUB6-LID Lid for SUB6SUB6-UT 6 x 7.5 cm Running Tray w/Silicone Gasket SUB6-CS Colored Loading Strips–6 pcsSUB6-GS Gel Scoop for SUB6SUB-LEAD Replacement High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-FC External Adjustable Casting Unit

SUB6 Mini SubmarineAccessories and Replacement Parts

*Microtiter spacing for use with a multichannel pipette

SUB6-LID

Optional External,Adjustable Casting UnitThe flexibility of the SUB-FC offers aconvenient external casting systemcapable of accommodating thecomplete range of Hoefer 6-15 cmwide running trays, increasingthroughput and efficiency by enablingthe tank to run concurrently.

SUB6-UT

SUB6-TANK

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SUB10 Mini-Plus Submarine Gel Electrophoresis UnitIdeal for routine preparatory and analytical electrophoresis techniques




UV-transparent running tray–allowsthe user to image the gel withoutrisk of damage due to handling

Side handles–for safe and easy transportation around the laboratory

Ordering InformationCat. # DescriptionSUB10 Mini-Plus Submarine Gel Unit

Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . 10 x 11.5 cmMaximum Buffer Volume . . . . . . . . . 450 mlMaximum Power Settings . . . . . . . . . 300 V, 200 mA, 60 WMaximum Sample Capacity . . . . . . . 80Maximum Running Temperature . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . 16.5 x 23 x 6.5 cmSafety Certifications . . . . . . . . . . . . . EN61010-1, CE

Includes:• Buffer Chamber Assembly • Running Tray w/Silicone Gasket• Safety Lid • 1 mm thick, 16 well combs–2 pcs• High Voltage Leads • Colored Loading Strips

The SUB10 Mini-Plus Horizontal Gel Unit has a maximum80-sample throughput capacity and features a removable gel-

casting tray with end gaskets that allow gels to be cast directlywithin the tank.

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Accessories and Replacement Parts Cat. # DescriptionSUB10-TANK Buffer Chamber AssemblySUB10-LID Lid for SUB10SUB10-UT 10 x 11.5 cm Running Tray w/Silicone GasketSUB10-CS Colored Loading Strips–6 pcsSUB10-GS Gel Scoop for SUB10SUB-LEAD Replacement High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-FC External Adjustable Casting Unit

SUB10 Mini-Plus SubmarineAccessories and Replacement Parts


(mm) (mm) Deep Well (µl)SUB10-C1-8 8 1 9 40SUB10-C1.5-8 8 1.5 9 60SUB10-C2-8 8 2 9 80SUB10-C1-10MC 10* 1 7 30SUB10-C1.5-10MC 10* 1.5 7 45SUB10-C2-10MC 10* 2 7 60SUB10-C1-12 12 1 5.5 25SUB10-C1.5-12 12 1.5 5.5 35SUB10-C2-12 12 2 5.5 50SUB10-C1-16 16 1 3.6 15SUB10-C1.5-16 16 1.5 3.6 25SUB10-C2-16 16 2 3.6 30SUB10-C1-20MC 20* 1 3 12SUB10-C1.5-20MC 20* 1.5 3 20SUB10-C2-20MC 20* 2 3 25


SUB10-LID

SUB10-TANK

SUB10-UT

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SUB13 Midi Submarine GelElectrophoresis Unit

Ideal for analytical and preparative studiesof nucleic acids


Buffer recirculation ports–may beconnected to a peristaltic pump(see page 142) for buffer recircu-lation during electrophoresis tomaintain buffer pH and preventionic gradient formation



UV-transparent running tray– allowsthe user to image the gel without riskof damage due to handling

Side handles–for safe and easy trans-portation around the laboratory

Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . 12.8 x 15 cmMaximum Buffer Volume . . . . . . . . . . 900 mlMaximum Power Settings . . . . . . . . . 300 V, 200 mA, 60 WMaximum Sample Capacity . . . . . . . 112Maximum Running Temperature . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . . 20 x 32 x 7 cmSafety Certifications . . . . . . . . . . . . . . EN61010-1, CE

Ordering InformationCat. # DescriptionSUB13 Midi Submarine Gel Unit

Includes:• Buffer Chamber Assembly • 1 mm thick, 16 well combs–2 pcs• Safety Lid • Colored Loading Strips• High Voltage Leads • Buffer Recirculation Ports–2 pcs• Running Tray w/Silicone Gasket

The SUB13 Midi Submarine Gel Unit has a maximum112-sample throughput capacity and features a removable

gel-casting tray with end gaskets that allow gels to be castdirectly within the tank.

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Accessories and Replacement PartsCat. # DescriptionSUB13-TANK Buffer Chamber AssemblySUB13-LID Lid for SUB13SUB13-UT 12.8 x 15 cm Running Tray w/Silicone GasketSUB13-CS Colored Loading Strips–6 pcsSUB13-GS Gel Scoop for SUB13SUB-LEAD High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-BRP Buffer Recirculation Ports–2 pcsSUB-FC External Adjustable Casting Unit

SUB13 Midi SubmarineAccessories and Replacement PartsSUB13 CombsCat. # # of Wells Thickness Well Width Volume in 5 mm

(mm) (mm) Deep Well (µl)SUB13-C1-10 10 1 9.5 40SUB13-C1.5-10 10 1.5 9.5 60SUB13-C2-10 10 2 9.5 85SUB13-C1-12MC 12* 1 8 35SUB13-C1.5-12MC 12* 1.5 8 50SUB13-C2-12MC 12* 2 8 70SUB13-C1-16 16 1 5.5 25SUB13-C1.5-16 16 1.5 5.5 35SUB13-C2-16 16 2 5.5 50SUB13-C1-20 20 1 4 17SUB13-C1.5-20 20 1.5 4 25SUB13-C2-20 20 2 4 35SUB13-C1-24 24 1 3 13SUB13-C1.5-24 24 1.5 3 20SUB13-C2-24 24 2 3 25SUB13-C1-28MC 28* 1 3 13SUB13-C1.5-28MC 28* 1.5 3 20SUB13-C2-28MC 28* 2 3 25


SUB13-UT

SUB13-LID

SUB13-TANK

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SUB15 Standard Submarine GelElectrophoresis Unit

Provides the most commonly used tray size and comb options

Features and BenefitsThe market standard–a 15 x 15 cm geltray, with comb options ranging from1 to 30 samples, is currently the mostpopular format in today’s market

Multichannel pipette compatiblecombs–with a maximum 30 samplethroughput reduce gel loading time,while preparatory combs enablenucleic acids to be scaled-up forcloning

Color-coded and height adjustablecombs–easily identify comb thicknessat a glance and control well depth

Buffer recirculation ports–may beconnected to a peristaltic pump(see page 142) for buffer recirculationduring electrophoresis to maintainbuffer pH and prevent ionicgradient formation


UV-transparent running tray–allowsthe user to image the gel without riskof damage due to handling


Ordering InformationCat. # DescriptionSUB15 Standard Submarine Gel Unit

Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . 15 x 15 cmMaximum Buffer Volume . . . . . . . . . 1.2 LMaximum Power Settings . . . . . . . . . 300 V, 200 mA, 60 WMaximum Sample Capacity . . . . . . . 120Maximum Running Temperature . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . 21.5 x 33.5 x 7cmSafety Certifications . . . . . . . . . . . . . EN61010-1, CE

Includes:• Buffer Chamber Assembly • 1 mm thick, 16 well combs–2 pcs• Safety Lid • Colored Loading Strips• High Voltage Leads • Buffer Recirculation Ports–2 pcs• Running Tray w/Casting Gates

A maximum 120-sample throughput makes the SUB15Standard Gel Unit a highly versatile option for screeningsamples ranging from DNA mini-preps to PCR products.

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Accessories and Replacement Parts Cat. # DescriptionSUB15-TANK Buffer Chamber AssemblySUB15-LID Lid for SUB15SUB15-UT 15 x 15 cm Running Tray w/Casting GatesSUB15-CG Casting Gates w/Silicone Gasket–2 pcsSUB15-CS Colored Loading Strips–6 pcsSUB15-GS Gel Scoop for SUB15SUB-LEAD Replacement High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-BRP Buffer Recirculation Ports–2 pcsSUB-FC External Adjustable Casting Unit

SUB15 Standard SubmarineAccessories and Replacement Parts

SUB15 Casting GatesThe included casting gates conven-iently and efficiently seal the runningtray without the hassles of using tape.


(mm) (mm) Deep Well (µl)SUB15-C1-10 10 1 12.8 64SUB15-C1.5-10 10 1.5 12.8 96 SUB15-C2-10 10 2 12.8 128 SUB15-C1-16MC 16* 1 6.4 32 SUB15-C1.5-16MC 16* 1.5 6.4 48 SUB15-C2-16MC 16* 2 6.4 64 SUB15-C1-20 20 1 5.9 29 SUB15-C1.5-20 20 1.5 5.9 43 SUB15-C2-20 20 2 5.9 58 SUB15-C1-25 25 1 3.5 17 SUB15-C1.5-25 25 1.5 3.5 25 SUB15-C2-25 25 2 3.5 34 SUB15-C1-30MC 30* 1 2.5 12 SUB15-C1.5-30MC 30* 1.5 2.5 18 SUB15-C2-30MC 30* 2 2.5 24

SUB15-LID

Optional External,Adjustable Casting UnitThe flexibility of the SUB-FC offers aconvenient external casting systemcapable of accommodating thecomplete range of Hoefer 6-15 cmwide running trays.

SUB15-UT

SUB15-TANK

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SUB20 Maxi-Standard andSUB20C Maxi-Cooled Submarine

Gel Electrophoresis UnitsIdeal for high resolution analytical and preparative studies of nucleic acids

Features and BenefitsLarge format–20 x 20 cm gel tray isideal for high resolution techniques

Multichannel pipette compatiblecombs–with a maximum 40 samplethroughput–reduce gel loading time

Color-coded and height adjustablecombs–easily identify combthickness at a glance and controlwell depth





Ordering InformationCat. # DescriptionSUB20 Maxi-Standard Submarine Gel Unit

Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . 20 x 20 cmMaximum Buffer Volume . . . . . . . . . . 2.2 LMaximum Power Settings . . . . . . . . . 1000 V, 500 mA, 500 WMaximum Sample Capacity . . . . . . . . 160Maximum Running Temperature . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . . 27 x 47.5 x 8 cmSafety Certifications . . . . . . . . . . . . . . EN61010-1, CE


The SUB20 Maxi-Standard Submarine Electrophoresis Unitprovides a 20 x 20 cm gel-running tray with four comb

positions which allows the user to perform high-resolution analyticresearch for a maximum of 168 samples. The SUB20C Maxi-CooledSubmarine Electrophoresis Unit has the same efficient design as the

SUB20 with the addition of a cooling base that can beconnected to a recirculation bath allowing high voltageelectrophoretic separations without overheating.

Cat. # DescriptionSUB20C Maxi-Standard Submarine Gel Unit w/Cooling Base

Includes:• Buffer Chamber Assembly w/Cooling Ports • 1 mm thick, 16 well combs–2 pcs• Safety Lid • Colored Loading Strips• High Voltage Leads • Buffer Recirculation Ports–2 pcs• Running Tray w/Casting Gates

SUB20

SUB20C

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Additional Features andBenefits of the SUB20CCooled base–covering the entire20 x 20 cm gel tray–allowsseparations to be performed fasterand at higher voltage, without lossof resolution

Cooling ports–connect the cooledbase to an optional chiller forenhanced cooling (see page 140).

Accessories and Replacement PartsCat. # DescriptionSUB20-TANK Buffer Chamber AssemblySUB20C-TANK Buffer Chamber Assembly w/Cooling PortsSUB20-LID Lid for SUB20 and SUB20CSUB20-UT 20 x 20 cm Running Tray w/Casting GatesSUB20-CG Casting Gates w/Silicone Gasket–2 pcsSUB20-CS Colored Loading Strips–6 pcsSUB20-GS Gel Scoop for SUB20SUB-LEAD Replacement High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-BRP Buffer Recirculation Ports–2 pcs


(mm) (mm) Deep Well (µl)SUB20-C1-16 16 1 8.5 35SUB20-C1.5-16 16 1.5 8.5 55SUB20-C2-16 16 2 8.5 75SUB20-C1-20MC 20* 1 7 30SUB20-C1.5-20MC 20* 1.5 7 45SUB20-C2-20MC 20* 2 7 60SUB20-C1-28 28 1 5 20SUB20-C1.5-28 28 1.5 5 30SUB20-C2-28 28 2 5 40SUB20-C1-40MC 40* 1 3 13SUB20-C1.5-40MC 40* 1.5 3 19SUB20-C2-40MC 40* 2 3 25


SUB20 Casting GatesThe included casting gates convenientlyand efficiently seal the running traywithout the hassles of using tape.

SUB20 Maxi-Standard andSUB20C Maxi-Cooled Submarine Accessories and Replacement Parts

SUB20-UT

SUB20-LID

SUB20C-TANK

SUB20-TANK

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SUB25 Maxi-Plus Standard andSUB25C Maxi-Plus Cooled Submarine

Gel Electrophoresis UnitsIdeal for high throughput screening of nucleic acids, particularly PCR products and in genotyping studies

Features and BenefitsLarge format–25 x 30 cm gel tray isideal for high throughput techniques

Multichannel pipette compatiblecombs–with a maximum 52 samplethroughput–reduce gel loading time

Color-coded and height adjustablecombs–easily identify combthickness at a glance and controlwell depth





Ordering InformationCat. # DescriptionSUB25 Maxi-Plus Standard Submarine Gel Unit

Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . . . . . 25 x 30 cmMaximum Buffer Volume . . . . . . . . . . . . . 3 LMaximum Power Settings . . . . . . . . . . . . . 1000 V, 500 mA, 500 WMaximum Sample Capacity . . . . . . . . . . . 624Maximum Running Temperature . . . . . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . . . . . 35 x 56 x 9 cmSafety Certifications . . . . . . . . . . . . . . . . . EN61010-1, CE


The SUB25 Maxi-Standard Submarine Electrophoresis Unit provides a25 x 30 cm gel-running tray with 12 comb positions accommodatinga maximum throughput of 624 samples. The SUB25C Maxi-CooledSubmarine Electrophoresis Unit has the same efficient design asthe SUB25 with the addition of a cooling base that can beconnected to a recirculation bath allowing high voltageelectrophoretic separations without overheating.

Cat. # DescriptionSUB25C Maxi-Plus Standard Submarine Gel Unit w/Cooling Base

Includes:• Buffer Chamber Assembly w/Cooling Ports • 1 mm thick, 26 well combs–6 pcs• Safety Lid • Colored Loading Strips• High Voltage Leads • Buffer Recirculation Ports–2 pcs• Running Tray w/Casting Gates

SUB25

SUB25C

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Additional Features andBenefits of the SUB25CCooled base–covering the entire 25 x 30 cm gel tray–allowsseparations to be performed fasterand at higher voltage, without loss of resolution

Cooling ports–connect the cooledbase to an optional chiller forenhanced cooling (see page 140).

Accessories and Replacement PartsCat. # DescriptionSUB25-TANK Buffer Chamber AssemblySUB25C-TANK Buffer Chamber Assembly w/Cooling PortsSUB25-LID Lid SUB25 and SUB25CSUB25-UT 25 x 30 cm Running Tray w/Casting GatesSUB25-CG Casting Gates w/Silicone Gasket–2 pcsSUB25-CS Colored Loading Strips–6 pcsSUB25-GS Gel Scoop for SUB25SUB-LEAD Replacement High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-BRP Buffer Recirculation Ports–2 pcs


(mm) (mm) Deep Well (µl)SUB25-C1-26MC 26* 1 7 30SUB25-C1.5-26MC 26* 1.5 7 45SUB25-C2-26MC 26* 2 7 60SUB25-C1-52MC 52* 1 3 13SUB25-C1.5-52MC 52* 1.5 3 20SUB25-C2-52MC 52* 2 3 25

*Microtiter spacing for use with multichannel pipette

SUB25 Casting GatesThe included casting gates convenientlyand efficiently seal the running traywithout the hassles of using tape.

SUB25 Maxi-Plus Standardand SUB25C Maxi-Plus CooledSubmarine Accessories andReplacement Parts

SUB25-LID

SUB25-UT

SUB25-TANK

SUB25C-TANK

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NEW SUBHT High ThroughputSubmarine Gel Electrophoresis Unit

Ideal for high throughput screeningapplications like single strandpolymorphism (SSP) analysisfollowing PCR

Features and BenefitsFast, Error-Free Loading–Load anentire 96-well plate using a multi-channel pipette

Simplified Analysis–Fluorescent labelsfor each sample printed on a UVtransparent gel tray allows for instantsample identification (matched to a96-well plate) during post-run visualization

High Throughput–Run up to 120samples (96-well plate + 24 controland/or marker samples) in 30 minutes

Better Reproducibility–Eliminatevariability caused by running bufferpH drift and ionic gradient formationwith optional buffer recirculationcapability

Reduced Buffer Needs–Compact tankdesign needs less buffer to cover gelwhich not only reduces bufferconsumption but provides greatercontrol of voltage gradients and run times

Maximum Flexibility–Fullycompatible with a wide range ofcombs and accessories to meet allyour high throughput and standardgel analysis needs

Ordering InformationCat. # DescriptionSUBHT High Throughput Submarine Gel Unit

Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . 15 x 15 cmMaximum Buffer Volume . . . . . . . . . 1.2 LMaximum Power Settings . . . . . . . . . 300 V, 200 mA, 60 WMaximum Sample Capacity . . . . . . . 120Maximum Running Temperature . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . 21.5 x 33.5 x 7cmSafety Certifications . . . . . . . . . . . . . EN61010-1, CE

Includes:• Buffer Chamber Assembly • 1 mm thick, 30 well combs–4 pcs• Safety Lid • Colored Loading Strips• High Voltage Leads • Buffer Recirculation Ports–2 pcs• Fluorescent Labeled Running Trayw/Casting Gates

This high throughput horizontal gel unit can accommodate upto 120 samples and resolve DNA strands from 50-1000 basepairs in 30 minutes.

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Accessories and Replacement Parts Cat. # DescriptionSUBHT-TANK Buffer Chamber AssemblySUBHT-LID Lid for SUBHTSUBHT-LUT 15 x 15 cm Fluorescent Labeled Running Tray w/Casting GatesSUBHT-UT 15 x 15 cm Running Tray w/Casting GatesSUBHT-CG Casting Gates w/Silicone Gasket–2 pcsSUBHT-CS Colored Loading Strips–6 pcsSUBHT-GS Gel Scoop for SUBHTSUB-LEAD Replacement High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-BRP Buffer Recirculation Ports–2 pcsSUB-FC External Adjustable Casting UnitPP24 Mini-Peristaltic Pump, see page 142

SUBHT High Throughput SubmarineAccessories and Replacement Parts

SUBHT Casting GatesThe included casting gates conven-iently and efficiently seal the runningtray without the hassles of using tape.

SUBHT Compatible CombsCat. # # of Wells Thickness Well Width Volume in 5 mm

(mm) (mm) Deep Well (µl)SUB15-C1-10 10 1 12.8 64SUB15-C1.5-10 10 1.5 12.8 96 SUB15-C2-10 10 2 12.8 128 SUB15-C1-16MC 16* 1 6.4 32 SUB15-C1.5-16MC 16* 1.5 6.4 48 SUB15-C2-16MC 16* 2 6.4 64 SUB15-C1-20 20 1 5.9 29 SUB15-C1.5-20 20 1.5 5.9 43 SUB15-C2-20 20 2 5.9 58 SUB15-C1-25 25 1 3.5 17 SUB15-C1.5-25 25 1.5 3.5 25 SUB15-C2-25 25 2 3.5 34 SUB15-C1-30MC 30* 1 2.5 12 SUB15-C1.5-30MC 30* 1.5 2.5 18 SUB15-C2-30MC 30* 2 2.5 24

SUBHT-LID

Optional External,Adjustable Casting UnitThe flexibility of the SUB-FC offers aconvenient external casting systemcapable of accommodating thecomplete range of Hoefer 6-15 cmwide running trays.

SUBHT-UT

SUBHT-TANK

HE-PLUS Electrophoresis SystemComplete Horizontal Electrophoresis Systemdesigned to meet a wide range of research

application requirements

Technical SpecificationsUnit Dimensions (w x l x h) . . . . 24.5 x 17.0 x 6.2 cmGel Dimensions (w x l) . . . . . . . . 12.5 x 13.0 cmMaximum Sample Capacity . . . . 112 samples (4 combs, 28 samples each)Buffer Capacity . . . . . . . . . . . . . . 350 mlDistance Between Electrodes . . . 13.5 cmElectrophoresis TankOverall Dimensions (w x l x h) . . 18.3 x 16.4 x 5.6 cmMaterial Characteristic . . . . . . . . UV transmitting (50% at 254 nm, 80% at 312 nm)Solution Volume . . . . . . . . . . . . . 350 mlPower SupplyOverall Dimensions (w x l x h) . . 7.5 cm x 17.0 x 6.2 cmWeight . . . . . . . . . . . . . . . . . . . . 410 gInput Voltage . . . . . . . . . . . . . . . AC 100 – 240 V, 50/60 HzOutput Voltage . . . . . . . . . . . . . . 10 to 150 volts; constant peak voltage of 150 VOutput Amperage . . . . . . . . . . . . 10 to 400 mAMaximum Wattage . . . . . . . . . . . 45 WTimer . . . . . . . . . . . . . . . . . . . . . 99 hours 59 min, or continuous runSafety Switch . . . . . . . . . . . . . . . A microsensor in the power supply prevents

output without the safety lid in placeMemory Function . . . . . . . . . . . . Automatic memory (the last used Voltage & Time)Safety Certifications . . . . . . . . . . TVE, CSA, and CE

The new HE-PLUS is completely integrated with a built-in variable powersupply and provides a compact easy-to-use horizontal electrophoresis system for the separation of nucleic acids. An LED display enables easy viewing of thevoltage, amperage, and time. Also featured is a 99:59 minute timer with anaudible end of run alert.

The HE-PLUS offers a high throughput 96 well format with multichannel pipettecompatible combs for the direct loading of samples in a small footprint. The systemcomes complete with four 14/28 well combs, three gel trays, and an externalcasting stand. The horizontal tank is made of rugged polycarbonate and has avented lid to reduce condensation so the run can be visually monitored.

AdvantagesCompact and Easy to Use

Built-in Variable Power Supplywith LED Display

Adjustable Power Supply from10 to 400 mA or 10 to 150 Volts

Built-in Timer with Audible Alert

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HE-PLUS18

HE-PLUS16

Accessories and Replacement PartsCat. # DescriptionHE-PLUS11 Gel Trays Large 12.5 x 13 cm–2 pcsHE-PLUS12 Gel Trays Medium 12.5 x 6 cm–2 pcsHE-PLUS13 Gel Trays Mini 6 x 6 cm–4 pcsHE-PLUS1428 HE-PLUS Large/Medium Comb 14/28 wells–2 pcsHE-PLUS0508 HE-PLUS Mini Comb 5/8 wells–2pcsHE-PLUS16 HE-PLUS Mini Casting Kit; includes 4 gel trays, 2 combs

(5/8 wells) and casting standHE-PLUS17 HE-PLUS Casting Stand for all 3 gel sizesHE-PLUS18 HE-PLUS Standard Casting Kit; includes 1 large gel tray,

2 medium gel trays, 4 combs (14/28 Wells) and casting stand

Ordering InformationCat. # DescriptionHE-PLUS-115V HE-PLUS Electrophoresis Complete System 115VHE-PLUS-230V HE-PLUS Electrophoresis Complete System 230V

Includes:• Gel Tank• Safety Lid• Power Supply• Large Gel Tray

• Medium Gel Trays–2 pcs• Large/Medium Comb 14/28 Wells–4 pcs• Casting Stand

HE-PLUS Electrophoresis System(cont.)

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sold via in Benelux, Spain and Portugal

nucleic acid electrophoresis -...

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