nanotechnology in halal · pdf file... food which has been cultivated, produced, processed or...
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NANOTECHNOLOGY IN HALAL INDUSTRY A NEW FRONTIER IN TECHNOLOGY
FOR FOOD & AGRICULTURE INDUSTRY
Combinatorial Technology & CatalysisDirector
Nanotechnology & Catalysis Research Centre,“NANOCEN
Universiti Malaya, Malaysia<[email protected]>
PROF DR SHARIFAH BEE ABD HAMID
ForWORLD HALAL RESEARCH SUMMIT 6 – 7 April 2011
“ Catalyst for Growth in the Halal Industry ”
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“The term „nanofood‟ describes food which has been cultivated, produced, processed or packaged using nanotechnology techniques or tools, or to which manufactured nanomaterials have been added”Source: Joseph and Morrison (2006)
“ ability to work at the atomic, molecular and even sub-molecular levels in order to create and use material structures, devices and systems with new properties and functions”Source: National Science Foundation, USA
NANOTECHNOLOGY
NANOFOOD
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NANOSTRUCTURED MATERIAL
ENCAPSULATION & DELIVERY SYSTEM
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FOOD QUALITY MONITORING
Quality Assurance:• Consumers demand safe, free from contaminants & wholesome food,
authentic, fresh;• Compliance to Government regulations to ensure Food Safety & Feed Hygiene
TESTINGS•Food Labeling •Heavy Metals•Microbiological•Alcohols•Pesticides & herbicides
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Why Food SpoilsDefinition: Disagreeable change in a food normal state. Such changes can be detected by smell, taste, touch, or sight.
(microorganism, enzymes, oxygen)
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Applications
EnvironmentalFood & Health
AgricultureMedical diagnosis
Recognition principle Transducing mechanism
LuminescenceAbsorptionPolarizationFluorescence
ConductometricAmperometricPotentiometric Ion-sensitive
Acoustic waveMicrobalanceResonant
AntibodiesEnzyme
OrganelleVitamins
pHImmunological
analytesCell or tissue
Volatile gases & vapors ions
Nanosensor
DNAAntibody/antigen
Molecular recognitionEnzymaticBiomimetic
OpticalMass
ElectrochemicalPiezoelectric
Thermal
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Printed Indicators
Printable oxygen indicator•Inkjet printable on plastic•Low cost•Indicator "formed" at the point of packaging •Visual colour change or measurable change in code•"Used by“ indicator at home after opening the package back
( Heilmann et al. (2004) WO2004094262 )
O2 + TiO2
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CHEMICAL SENSOR: Electronic nose
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Freshness IndicatorsRF-readablespoilage sensor for poultry Response vs. storage time
nanolayer of Ag on an opaque carrier reacts with H2S
( Smolander et al. (2003) WO2003044521 )( Smolander et al. (2004) WO2004102185 )
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CHALLENGES IN FOOD WITH PRESENCE OF BACTERIA
SCENARIOS
Millions of people become ill due to food pathogen annually, and many suffer chronic complications and may even lead to death.
Bacteria Est Annual cases
Est Annual Hospitalizations
Est Annual Deaths
Infectious Dose (CFU)
Campyllobacter spp. 1,963,141 10,539 99 400 to 106
Salmonella 1,342,532 16,102 556 104 to 107
Staphylococcus 185,060 1,753 2 > 106
E coli (all types) 173,107 2,785 78 101 to 102
Clostridium perfringens
248,520 41 7 > 108
Listeria monocytogenes
2,493 2,298 499 400 to 103
Source: Economic Research Services, USDA
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Vision of Direct Bacterial Detection
Sprinkle sensors onto the tomato, wait 1 minute for binding to occur.
Measure wirelessly and simultaneously thesensors. Less than 1 minute.
undetectable amount of Salmonella- control and measurement show same resonance frequency
positive Salmonella Contamination- peaks appear below control
Retrieve sensor
1 2
34
( Li, S. et al. 2010. Detection & Food Safety Center, Auburn University )
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(Steve Tung, University of Arkansas)
E-COLI BACTERIA IDENTIFICATION
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(a) Au/Si nanorod fabrication; (b) dye immobilization onto the Si nanorods; (c) antibody conjugation onto the Au tip;(d) Salmonella detection via the antibody–antigen rxn
Salmonella detection by bio-functionalized Au/Si nanorods.
(Fu etal, 2008)
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Nano Sensors for Grain Storage
Pressure Sensor
Acoustic Sensors
Odour Sensor
Temperature Sensor
Carbon dioxide Sensor
Humidity and Dust SensorGrain Bin
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The purpose of food packaging is to increase food shelf life by avoiding•spoilage, •bacteria, or •the loss of food nutrient.
Nanotechnology offers higher hopes in food packaging by promising •longer shelf life, •safer packaging, •better traceability of food products, and healthier food.
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FOOD PACKAGING: NANO-enabled food contact material (FCM)
• Nanoparticle reinforced materials composites
Polymer [PA, Nylons, PS, PU, PET] composites + nanoclay
• Polymer composites with nano-metals or nano-oxides
Coating containing nano-particles
Antimicrobiol nano-emulsions (e.g. nanomicelle-based products containing glycerine & removes pesticides residues, and oil/dirt from cutlery)
• Coating containing Nanoparticles
Product: Kitchenware, cutting boards, teapots, etc.
Lightweight but strong
•Prolonged shelf-life of package foodstuff, restricts permeation of gases.
Antimicrobial action, abrasion resistance, UV absorption, and strength.
Active packaging (absorb O2 to keep food fresh)
UV absorbers to prevent UV degradation in plastics
•Low risk to consumers attributable to the fixed or embedded of ENM in plastic polymer
Low Cost
Communication with consumers as a marketing tool
Nano-Particles Benefits
• Used to create antimicrobial, scratch resistant, anti-reflective, or corrosion resistant surfaces.
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• Active (Intelligent) Packaging,
• Modified atmosphere packaging,
• Edible film and
• Edible coating(Source: Han, 2005)
• EDIBLE FILM : assemble of multilayered edible films or
coating (Jochen et al,2006)
• EDIBLE COATING : Coating and object with multilayer (Jochen et al,2006)
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Intelligent packaging
• Intelligent ,smart and active packaging system produced by nanotechnology be able to repair the tears and leakage
• Respond to environmental conditions change in moisture and temperature
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Time Temperature Indicator(TTI)
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Reinforcement of (Bio-) Polymer Based Packaging Materials- Nanoclay Composites
Inert Monolayers Protein Carbohydrates
Clayor syntheticpolymers
• Torturous Path• Reduced O2
and H2Otransmission
• Microwaveable• Increased
mechanicalstrength
Microphase separated IntercalatedExfoliated
Already commercialized (Weiss, J., 2007)
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Barrier PropertiesInorganic substances (e.g.nanoclay) can be used tointroduce oxygen barrierproperties to fibre-basedMaterials
Biomolecules (carbohydrates,proteins) goodoxygen barrierproperties in low RH
Improved performance withbiomolecules with tailorednanostructures
(Forssell et al (2002) Carboh Polymers, 47: 125129)(Partanen et al (2004) Carboh Polymers, 56: 147155)
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(A) Schematic showing exploded view of typical antimicrobial coating nanopackaging film; (B) antimicrobial active packaging microorganisms hydrolyses starch based particles causing release of the antimicrobial lysozyme resulting in inhibitors of microbial growth
(Suresh and Digvir,2010)Nanotechnology in Food Industries: An Opportunities
NANOPACKAGING: Antimicrobial Coating
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NANODELIVERY SYSTEMS BASED ON ENCAPSULATION TECHNOLOGY
Used to develop delivery systems for additives and supplements using carrier systems:
•Micelles
•Liposomes
•Bio-Polymer
Example
Vegetable oil enriched in vitamins, minerals, phytochemicals.
Slimming product based on cocoa nanocluster
Hydrolysed milk protein in Infant Formula –lactalbumin, a nutrient, supplement & pharmaceuticals.
• Preserving the ingredients and additives during processing & storage
• Masking unpleasant tastes and flavours
• Controlling the release of additives
• Better dispersion of water-insoluble food ingredients and additives
• Improve uptake of the encapsulated nutrients and supplements
Nano-EncapsulationBenefits
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Solid-Lipid Nanoparticles
Composite Nanofibers
Colloidosomes
Double Layered Liposome
Nanolaminates
Next Generation Nano-Encapsulation Systems
(Weiss, J., 2007)
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Mechanism of Encapsulation-Controlled Release
Microsphere
Active ingredient
Nanosphere
( source: Adi Shefer, Salvona Technologies Inc.)
Nanoencapsulation- a technology of packaging solids, liquids or gaseous materials in miniature, sealed capsules that can release their contents at controlled rates under specific conditions (Desai and Park, 2005).
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Incorporation of insulin by "co-precipitation" into CaCO3 microparticles. After polyelectrolyte (PE) multilayer coating the carbonate-cores are dissolved by EDTA treatment:
pH-Triggered Release of Encapsulated Insulin
PE multilayers
CaCO3 particles
dissolution1 M CaCl2 +
1 M Na2CO3
+
1 mg/ml insulin
CaCO3 particles with incorporated
insulin of up to 100mg of insulin
per 1 g of particles
PE Layer-by-layer coating: Use of
biocompaticle or biodegradable polymers. Shell
thickness 10-50nm
CaCO3 particles dissolution, insulin remains. PE shell provides defined insulin release.
(GMD BIO Advance Inc.)
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pH-Triggered for the Release of BIOACTIVE & NUTRACEUTICALS MOLECULES
- nanocapsules with tuna fish oil
- nanocapsules break only in the stomach
Source: Tip Top Bakery
‘Tip-Top Up’ - Omega 3 Bread Canola Active Oil
Source: Shemen Industries
- nanoencapsulation of fortified phytosterols
- reduce cholesterol intake by 14%
Some Bio-active & Nutraceuticals molecules :Lactobacillus, alpha-lactalbumin, Flavanoids, Phenolic compounds, Vitamines (A,B+, C, E, etc), CoQ10, Omega-3, Lycopenes, Linoleic acid, Isoflavones,
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Temperature-Triggered Release of Encapsulated TOM YUM Cube
lemongrass oil bergamot oil
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Nanostructures & NanoParticles in Food
• NanoEmulsion
• Emulsion Bilayers
• Double or Multiple Emulsions
• Surfactant
• Micelles
• Reverse Micelles
The size & structure of FOOD influence the functionality of foods by providing
•Reduced Quantity
•New Taste
•Improved Textures
•Consistency
•Stability of Emulsions
Type of Nanotextures Benefits
E.g. Low salt, Creamy but Low Fat, hence healthier option to consumers. Spreads, Mayonnaise, Yoghurt, Cream, Ice creams, etc
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Fibers
Nanoemulsions
Particles
Liposomes
Microemulsions
Nanotech Ingredient Technologies
(Weiss, J., 2007)
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Enzymatic Structure Modification
Na caseinate
Potential enzymes•Transferases (Tgase)•Oxidative enzymes (laccase, peroxidase etc.)
Potential enzymes•Transferases (Tgase)•Oxidative enzymes (laccase, peroxidase etc.)
( Lantto et al. (2005) J.Agric. Food Chem. 53:92319237 )( Autio et al. (2005) J.Agric. Food Chem. 53:10391045 )
Dairy products Meat products
Cereals
10 x hardnessincrease withcrosslinking enzyme
37oC, Control 37oC, Tgase
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N&N publications with East Asia for each sector as percentage in period of 2009
In Malaysia
NANOFOOD ACTIVITIES
R&D PUBLICATIONSUSA = 1st
China = 18% (2nd )Active Players: India, Iran, S.Korea, Thailand.
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FOOD INDUSTRY
MATERIALS
PRODUCTPROCESSING
FOOD SAFETY &BIOSECURITY
FOOD TRACKING
Formulation
Packaging
Delivery
Nanosensors
Nanotraces
Molecularsynthesis
Nanobiotechnology
Nanoparticles
Nanostructuredmaterial
Nanocomposites
Nanoemulsions
Nanoscale reactionEngineering
Heat/ MassTransfer
Possibilities of Nanotechnology inFood Industry
The Next Big Thing is
……………………very small
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ACKNOWLEDGEMENT
Poopak & Nazirah, Fatimah Zahara
COMBICAT Team (University Malaya)
Nanotechnology & Catalysis R&D Centre (University Malaya)
NanoC Sdn Bhd
THANK YOU
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