C. VREULS, A. GENIN, G. ZOCCHI, G. GARITTE,C. VREULS, A. GENIN, G. ZOCCHI, G. GARITTE,C. ARCHAMBEAU, C. VAN DE WEERDT, J. MARTIAL C. ARCHAMBEAU, C. VAN DE WEERDT, J. MARTIAL

BMGGBMGG-CERM--CERM-ArArcelorMittalcelorMittal Research Ličge Research Ličge Director: J. MARTIALDirector: J. MARTIALJ.Martial@ulg.ac.beJ.Martial@ulg.ac.be

Nanocoatings of inorganic surfaces by molecular biomimetic

Strategies for surface coating:Bottom-up hierarchic assembly using engineered proteins

Molecular Biomimetic: Conception of new materials that imitate, or learn from nature at molecular scale

Aim of the Biocoat project (PPP, RW): To develop new functionalities with high added value on inorganic substrates through multifunctional molecular coatings following a multidisciplinary biomimetic approach

Interests of GEPIs : high specificity for- chemical composition - structuration (powder, surface…) - cristallographic form

GEPI Genetically Engineerd Polypeptide for Inorganic

Recognition => binding protein - inorganic material

Nature Molecular biomimetic

COPY

NATURE

1st step : isolate GEPI to gather incompatible materials

small peptide that recognizes and specifically binds an inorganic surface

GEPI: Linker betweeninorganic-organic materials

Selection of GEPI by Phage Display technology

1: Librairy presentation; 2: Wash; 3: Elution; 4: Amplification; 5: next round of selection; 6: sequencing after 3-4 rounds of selection

Inorganic-binding peptides as tools for surface quality control GEPI applications

Team’s Publications & Patent ApplicationsAll-in-one strategy for the fabrication of antimicrobial biomimetic films on stainless steel. J. Mat. Chem. 2009 19: 4117-4125.Inorganic-binding peptides as tools for surface quality control J. Inorganic Biochem. 2010Plasma deposition of antimicrobial peptides onto stainless steel for the prevention of bacterial biofilms (submitted to J. Mat. Chem)Biomolecules in multilayer film for antimicrobial and easy-cleaning stainless steel surface applications (submitted to Biofouling)Multifunctional Coatings WO 2009/147007 A2Inorganic-binding peptides as tools for surfaces quality control detection PCT/EP2009/004876).

ZnO-binding peptide with FITC at the N-terminal

Galva Gi206031 Galva DRX51

Ag2O

100 µm

ZnO

Ag2O

ZnO

Patterned substrate: 50-nm layer of Ag on pure zinc

2 types of galvanized steel

Control quality of raw or coated inorganic surface by fluorescence analyses

Acknowledgments The research was partly supported by the Walloon Region (PPP program BIOCOAT). We thank the BIOCOAT team members for their contribution, in particular Fabrice Farina (ArcelorMittal Research Industry, Liege, Belgium) for providing the various inorganic material coupons, GIGA-R, Systems Biology and Chemical Biology Unit (ULg)

Perspectives

Use Gepi for powder sorting, Surface fonctionalization…