C. VREULS, A. GENIN, G. ZOCCHI, G. GARITTE,C. VREULS, A. GENIN, G. ZOCCHI, G. GARITTE,C. ARCHAMBEAU, C. VAN DE WEERDT, J. MARTIAL C. ARCHAMBEAU, C. VAN DE WEERDT, J. MARTIAL
BMGGBMGG-CERM--CERM-ArArcelorMittalcelorMittal Research Ličge Research Ličge Director: J. MARTIALDirector: J. [email protected]@ulg.ac.be
Nanocoatings of inorganic surfaces by molecular biomimetic
Strategies for surface coating:Bottom-up hierarchic assembly using engineered proteins
Molecular Biomimetic: Conception of new materials that imitate, or learn from nature at molecular scale
Aim of the Biocoat project (PPP, RW): To develop new functionalities with high added value on inorganic substrates through multifunctional molecular coatings following a multidisciplinary biomimetic approach
Interests of GEPIs : high specificity for- chemical composition - structuration (powder, surface…) - cristallographic form
GEPI Genetically Engineerd Polypeptide for Inorganic
Recognition => binding protein - inorganic material
Nature Molecular biomimetic
COPY
NATURE
1st step : isolate GEPI to gather incompatible materials
small peptide that recognizes and specifically binds an inorganic surface
GEPI: Linker betweeninorganic-organic materials
Selection of GEPI by Phage Display technology
1: Librairy presentation; 2: Wash; 3: Elution; 4: Amplification; 5: next round of selection; 6: sequencing after 3-4 rounds of selection
Inorganic-binding peptides as tools for surface quality control GEPI applications
Team’s Publications & Patent ApplicationsAll-in-one strategy for the fabrication of antimicrobial biomimetic films on stainless steel. J. Mat. Chem. 2009 19: 4117-4125.Inorganic-binding peptides as tools for surface quality control J. Inorganic Biochem. 2010Plasma deposition of antimicrobial peptides onto stainless steel for the prevention of bacterial biofilms (submitted to J. Mat. Chem)Biomolecules in multilayer film for antimicrobial and easy-cleaning stainless steel surface applications (submitted to Biofouling)Multifunctional Coatings WO 2009/147007 A2Inorganic-binding peptides as tools for surfaces quality control detection PCT/EP2009/004876).
ZnO-binding peptide with FITC at the N-terminal
Galva Gi206031 Galva DRX51
Ag2O
100 µm
ZnO
Ag2O
ZnO
Patterned substrate: 50-nm layer of Ag on pure zinc
2 types of galvanized steel
Control quality of raw or coated inorganic surface by fluorescence analyses
Acknowledgments The research was partly supported by the Walloon Region (PPP program BIOCOAT). We thank the BIOCOAT team members for their contribution, in particular Fabrice Farina (ArcelorMittal Research Industry, Liege, Belgium) for providing the various inorganic material coupons, GIGA-R, Systems Biology and Chemical Biology Unit (ULg)
Perspectives
Use Gepi for powder sorting, Surface fonctionalization…