mycoplasma. mycoplasma has many different shapes because the microbe is absent of cell wall....
TRANSCRIPT
![Page 1: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/1.jpg)
Mycoplasma
![Page 2: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/2.jpg)
![Page 3: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/3.jpg)
![Page 4: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/4.jpg)
• Mycoplasma has many different shapes because the microbe is absent of cell wall.
Morphology and Staining
![Page 5: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/5.jpg)
Shapes of Mycoplasma by scanning electron microscopy
![Page 6: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/6.jpg)
Blue color by Giemsa stain
![Page 7: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/7.jpg)
• Mycoplasma is the smallest (0.2-0.3 µm) microbe that can grow on artificial cell-free-media and form very small colony on plate.
• Mycoplasma grow slowly in blood or serum contained media and produce "fried egg" colonies on agar plates.
• Due to the slow growth, the colonies need 3 weeks to develop. The colonies are extremely small.
Culture
![Page 8: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/8.jpg)
![Page 9: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/9.jpg)
Human pathogens
• There are many species in Mycoplasma in nature. Two genera are recognized as human pathogens: – Mycoplasma – Ureaplasma
• Mycoplasma pneumoniae • Ureaplasma urealyticum
![Page 10: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/10.jpg)
Mycoplasma pneumoniae
• cause primarily atypical pneumonia in human
![Page 11: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/11.jpg)
Virulent factors
• P1 protein– an adhering membrane protein
• Glycolipid antigen– Induce immunopathological injury
• Capsule– resists phagocytosis and display cytotoxicity
![Page 12: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/12.jpg)
Ureaplasma urealyticum
• Causes non-gonococcal urethritis (NGU). NGU is a common STD with or without clinical symptoms.
![Page 13: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/13.jpg)
Virulent factors
• Phospholipase
• Urease
• IgA protease
![Page 14: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/14.jpg)
◇ Laboratory diagnosis: Sample: Sputum or throat washings. Microscopy: Direct observation. Serological examination: Cold agglutinin test (IgM auto-antibody in sera of patients) and ELISA (P1 protein as the antigen).
![Page 15: Mycoplasma. Mycoplasma has many different shapes because the microbe is absent of cell wall. Morphology and Staining](https://reader036.vdocuments.us/reader036/viewer/2022062314/56649f395503460f94c56305/html5/thumbnails/15.jpg)
◇ Treatment: Since Mycoplasma lacks cell wall, the penicillins and cephalosporins are ineffective. The common used antibiotics are tetracycline and erythromycin.◇ Prevention: No vaccines are currently available