multilocus sequence typing of bacteria: a genotypic tool for strain selection aundy kumar, *jos m....
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Multilocus Sequence Typing of bacteria:a genotypic tool for strain selection
Aundy Kumar, *Jos M. Raaijmakers, Santhosh J Eapen
Indian Institute of Spices Research, Calicut
___________________________________________________________________**Laboratory of Phytopathology, Wageningen University,
The Netherlands
Image courtesy: pseudomonas.com
Tools and web resources at your door step!
Advances in sequencing methodsEver reducing cost of sequencingFreely available analytical toolsGood databases with constant updates
Full genome information on 1400 bacteria
http://pubmlst.org/http://www.mlst.net/http://www.pasteur.fr/recherche/genopole/PF8/mlst/
PseudomonasAcinetobacterKlebsiellaStenotrophomonasEnterobacterBacillus Burkholderia
MLST scheme has been developed for ~40 bacteria so far
Frequently encountered ones
What is MLST?
A nucleotide sequence based approach to uncover
allelic variants in conserved genes (house keeping)
for the purpose of characterizing, subtyping, and
classifying members of bacterial populations.
Proposed in 1998 using the human pathogen
Neisseria meningitidis as an example
A portable, universal, and definitive method for
characterizing bacteria
MLST – genes used
Pseudomonas aerugenosaacsA acetyl coenzyme AaroE Shikimate dehydrogenase
guaA GMP synthasemutL DNA mismatch repair proteinnuoD NADH dehydrogenase IppsA Phosphoenol pyruvate
synthasetrpE Anthralite synthetase
Ralstonia solanacearum
Glutamate dehydrogenase oxidoreductase
gdhA
Adenylate kinase adk
DNA gyrase subunit B gyrB
Glyceraldehyde 3-phosphate dehydrogeanase oxidoreductase
gapA
Phosphoenol pyruvate synthase
ppsA
Flagellin protein fli C
Regulatory transcription regulator
hrpB
Endoglucanase precursor
egl
PCR amplification of selected genes
Elution & purification of PCR product
Sequencing of PCR product
Sequence assembly (DNA Baser)
Blast the assembled sequence in database www.PAMDB.org
Assigning allele numbers for each gene
Unique allelic profile is called the Sequence Type(ST)
PROTOCOL
Each sequence for a given locus is screened for identity (not for similarity) with already known sequences for that locus. If the sequence is different, it is considered to be a new allele and is assigned a unique (arbitrary) allele number
Case study of Multilocus Sequence Typing of plant associated Pseudomonas aeruginosa
In Spice crops
Projects on bacterial endophytes in black pepper/ginger against major soil borne pathogens
…has yielded several diverse group of bacterial species
The best among the lot is
Pseudomonas aeruginosa
Aravind et al Letters in Applied Microbiology, 2009
Strain Source Year of collection
Isolated by
Pa-GEB9 Ginger, Calicut (against Pythium)
2007 Aundy Kumar
Pa-IISR51 Ginger, Karnataka (against Pythium)
1998 Aundy Kumar
Pa-BP35 Black pepper, Kerala (against Phytophthora)
2006 Aundy Kumar
Pa-IISR13 Black pepper, Andhra Pradesh (against Phytophthora)
1998 Y.R.Sarma
Pa-IISR6 Black pepper, Kerala (against Phytophthora)
2000 Y.R.Sarma
Pa-IISR853
Chromolaena odorata (against nematodes)
2005 S.J.Eapen
PAO1- Type strain
Human burn patient, Australia
1950s Bruce Holloway
Selected from several collection in various projects during different points
Some of them are endophytes in plants
…b
ackg
rou
nd
0
10
20
30
40
50
60
70
80
90
100
10 9 8 7 6 5 4 3 2 0
Bacterial concentration in suspension (log CFU per mL)
Lesi
on in
hibi
tion
(%)
0
1
2
3
4
5
6
7
8
Bacterial population in shoot
as endophyte (log CFU per
gram of shoot)
Inhibition of P. capsici induced les ion (%) by BP35 strain
Endophytic population (Log CFU g-1)
DN
A of
BP3
5
DNA extracted from bacterized stem is used as template
578bp
Direct evidence for protection of black pepper
by specific density of P. aeruginosa BP35 cells
PhytophthoraSuppression
90-100%
BP35Density in Suspension
8-10 log units
EndogenousPopulation of
BP354.5-6.5 log
units
Phenotype- functional
Leaf
Roots
StemLocalization of BP35::gfp2 in tomato
Accumulation of bacterial cells
Tracking of bacterium PaBP35
15 Days Post Bacterization in Five weeks old tomato cultivar, Moneymaker
Tissues fixed in 4% paraformaldehyde
Localized in aerial plant parts
The bacterium was tagged with gpf using Tn7 system
Phenotype- functional
Few of them are in field evaluation stage for
1. Phytophthora suppression2. Radopholus similis suppression
Single or Two nodal cutting are Bioprimed
Bacterized shoots
March 2010
July 2010
August 2010
Metalaxyl
Collectively they may be classified as Plant Associated Pseudomonas aeruginosa
Pseudomonas aeruginosa-
Type strain for Pseudomonas
Most intensively studied bacterium
Opportunistic human pathogen
Full genome published in Nature
MLST analysis
Analysis of housekeeping genes that are scattered in the genome
www.pseudomonas.com
Gene Amplicon (bp)
Position in the PAO1 genome
Sequence for MLST analysis (bp)
Coordinates in the PAO1 genome
No of alleles documented so far
acsA -(acetyl coenzyme A)
842 969670 390 970604- 971033
145
aroE-(Shikimate dehydrogenase)
1042 26711 495 26883-27380
146
guaA-(GMP synthase) 940 4227237 372 4226567-4226155 115
mutL-(DNA mismatch repair protein)
940 5551681 441 5551179- 5550698
124
nuoD- (NADH dehydrogenase I)
1042 2983963 366 2984570- 2984955
86
ppsA- (Phosphoenol pyruvate synthase)
989 1914037 369 1914994- 1915403
97
trpE- Anthralite synthetase
811 670980 441 671811- 672293
129
Choice of genes for MLST analysis
Genes were amplified using specific primers
The amplicon were eluted using gel elution kit (Sigma Aldrich)
Sequenced (Both forward and reverse run)
M: DNA ladder 50bp
aro
E
acsA
gu
aA
mu
tL
nu
oD
pp
sA
trp
E
BP3
5
PAO
1
acs
A a
roE
guaA
mutL
nuo
D ppsA
trpE
acs
A a
roE
guaA
mutL
nuo
D ppsA
trpE
1000bp
Out group from clinical origin
Gene amplification, isolation, sequencing and sequence analysis
aro
E
acsA
gu
aA
m
utL
n
uoD
p
psA
trp
E aro
E
acsA
gu
aA
m
utL
n
uoD
p
psA
trp
E
BP35 IISR 853 GEB 9
aro
E
acsA
gu
aA
mu
tL
nu
oD
p
psA
trp
Earo
E
acsA
gu
aA
m
utL
n
uoD
p
psA
trp
E aro
E
acsA
gu
aA
mu
tL
nu
oD
p
psA
trp
E
aro
E
acsA
gu
aA
m
utL
n
uoD
p
psA
trp
E
IISR 6 IISR 51 IISR 13
All seven genes were
isolated, sequenced
and analysed
The sequences were assembled and analysed using Vector NTiSequence viewer/DNA baser
Alleles were determined using pubmlst.org
String of alleles (Sequence Type) were analysed using eBurst algorithm (eBurst V3) or profuse algorithm (goeBurst.org)
>acsA-IISR6TATAACTGTNGGNTTNAACGCGCTGACCCACGAGCGGGTCTTCGACTACCGTCCGGGCGAAGTCTACTGGTGCACCCGCCGGACATCGGCTGGGTCACCGGCCACACCTACATCGTCTATGGCCCGCTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGCCGAACTACCCCGACGTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGCGCCGACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGACGGTTCCAGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGTGGTACTACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCAGACCGAGACCGGCGCCTGCCTGATGACCCCGCTGCCGGGCGCCCACGCGATGAAGCCGGGCTCTGCAGCCAAGCCGTTCTTCGGCGTGGTACCGGCGCTGGTGGACAACCTCGGCAACCTGATCGAGGGCGCCGCCGAGGGCAACCTGGTGATCCTCGACTCCTGGCCGGGCCAGGCGCGGACCCTGTTCGGCGACCATGACCGCTTCGTCGACACCTACTTCAAGACCTTCAAGGGCATGTACTTCACCGGCGACGGCGCGCGCCGCGACGAGGACGGCTACTACTGGATCACCGGGCGGGTCGACGACGTGCTCAACGTCTCCGGCCACCGCATGGGCACCGCCGAGGTGGAAAGCGCGATGGTCGCCCACCCGAAGGTCGCCGAGGCGGCGGTGGTCGGCATGCAGCACGACATCAAGGGGAGCG
Allele: 15
>aroE-IISR6CATGTCAGATGGAAACCGATTGAGACGTACGTCTCAAATGCTGCCTGACAACTCAGACAGTTGCCAATTGCCGGCGCAGCGTCTCCAACACTGGCGCCGAGGCAGGACGCACGCCACGCCAGAGGAAGAATGCCTCGGCGGCCTGCTCCACCAGCATGCCCAGGCCATCCAGGGTACGCGCCGCACCGCGTTCGGCGGCCCAGCGGTTGAAGGCGGTCGGTTCCTTGGCATACATCATGTCGTAGCAAACGGTACGGCCGGGCTCGATCACGCTCTGCGCCAGCGGCGGCACGTCGCCGGCAAGACTGGCCGAGGTGCCGTTGACGACCAGGTCGAAAGGCCCTTCGACCTCGGCGAAACCGCAGCCGCGCACCGCGCCGAGATCGGCGAACCGCTCGGCCAGATCCACGGCCTTCCGCGCCGTGCGGTTGGCGATCAGCAGCTCCGCCGGGCACTCGCCGAGGAAGGGTTCGAGCACCCCGCGCACCGCACCGCCGGCGCCGAGCAGGAGAACCCGCTTGCCGCGCAGCTCGACCCCGGCGTTCGCCGTCAGGTCCCGCAGCAAGCCCGCGCCGTCGGTGTTGTCGCCGCGCAGGCGACCGTCGGCCAGGCGGATGAGGGTGTTCACCGCCCCGGCCCGGGTGGCCCGCTCGCTCAATTCGTCCACCAGACGATAGGCCTCTTCCTTGAACGGCACGGTGACATTGGCGCCCTTGCCCTGCTCGAAGAAGCGCCGGGCGAACCCGGGGAAATCGTCCAGCGGCGCCAGCTGCGCGTCATAGACCAGCGCCTCGCCGGTCTGCTCGGCGAACAGGCGGTGGATCAGCGGCGACTTGCTGTGGCCGATGGGGTTGCCGAATACGCAATAGCGGTCCATGGGTCGTTCCTTTGCCGGGGTGGGACGGGACGAGCGGACTCGTCCCCTGCGGGCGGGTCAGGGCTGGCCGGCCAGCCAATCGCGGGTCGGCGAGGAAATACTCGGTCAGGCGCGCTTCCTCGCTGC
Allele: 121
>guaA-IISR6ANTNGNGGNCGNAACCNAANGNTGCCGCGGCCTGNCTTGGTGTTGGGTGACTTCCGGGGGGAATTCCAGCCGTTGNTGGCGCGGGAATTCGTNCCTTNAAGCGGTGGTGAGCCACGGCGACAAGGTCACCGAGATGCCGGCCGCGCTTCCACATCCTGGCCAGCACCCCGAGCTGCCCGATCGCCGCCATGGCAGACGATGCCCGCGCCTACTACGGCGTGCAATTCCACCCGGAAGTCACCCACACCAAGCAGGGCCTGCGCATTCTCTCGCGCTTCGTCCTCGACATCTGCGGTTGCGCCGCGCTGTGGACCCCCTCGAACATCGTCGACGACGCCATCGCCACCGTGCGCGCCCAGGTCGGTTCCTCCAAGGTCCTGCTAGGCCTCTCCGGCGGCGTGGACTCCTCGGTGGTCGCCGCGCTGCTGCACAAGGCCATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACGAAGGCGACCAGGTGATGGCCATGTTCGCCGAGAACATGGGCGTGAAGGTGATCCGCGCCAACGCCGAGGACAAGTTCCTCGGCCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGCAAGATCATCGGCCGCACCTTCATCGAAGTCTTCGACGAAGAAGCCACCAAGCTGCAGGACGTGAAGTTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCGGCCGGCGCCAAGACCGGCAAGGCCCACGTGATCAAGTCGCACCACAACGTCGGCGGCCTGCCGGAGGACATGCAGTTCGAACTGGTCGAGCCGCTGCGCGAACTGTTCAAGGACGAAGTGCGCAAGATCGGCCTGGAGCTGGGCCTGCCCTACGACATGGTCTACCGCCACCCGTTCCCCGGCCCGGGCCTGGGCGTGCGCATCCTCGGCGAGGTGAAGAAAGAGTACGCCGACCTGCTGCGCCAGGCCGACCACATCTTCATCGAGGAACTGCGCGCCTTCGACTGTACCACAGACAGCCCGGGCGGTTCGATACANTGCNNCGCCTACNACGGCNGGCAATTCCGCCCNGAAGTCACTCACACAAGCANNNNGGCCANNCTCTGCCNGTTCCN
Allele: 36
>mutL-IISR6GGCGGTCGCGAGTCATGGTNGCGCGNACCGAACTAATCGAGCCAGCGCTCGCCACGGAGCCGNGGCTCATCCACCCGCCTTCAGAGANATGCGTCGCGTGAGTGGTCGAGCGTCCCGCCTCGGTGGCCAAGGAACTCTGGAAAACAGCTTGACGCCGGTTCCCGGGCGCATTGATGTGGAGGTCGAGCAGGGCGGCATCAAGTTGCTGCGAGTGCGCGACGACGGTCGCGGCATCCCCGCCGACGACCTGCCGCTGGCCCTGGCTCGCCACGCCACCAGCAAGATCCGCGAGCTGGAAGACCTGGAGCGGGTGATGAGCCTCGGCTTCCGTGGCGAGGCGCTGGCCTCGATCAGTTCGGTAGCGCGCCTGACCATGACCTCGCGTACCGCCGACGCCGGCGAAGCCTGGCAGGTGGAAACCGAGGGCCGTGACATGCAGCCGCGGGTACAGCCGGCGGCGCACCCGGTGGGGACCAGCGTCGAGGTTCGCGACCTGTTCTTCAACACCCCGGCGCGGCGCAAGTTCCTGCGCGCCGAGAAGACCGAGTTCGACCATCTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCACCTGCGCCACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGCGCGCGCCGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAGGTCGAGCGCAACGGCCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCCAGCCGGACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCCACGCGGTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCTGTTCTTCGAAGTCGATCCGGCGGTTGGTGGACGTCANNTGCACCCGAACNGCACGAGTTCGCTTNNNGACAGCCGGATGNCCATGACCTTCCCTCTNNNGAGANACCCNNNGA
>nuoD-IISR6NTNNNGCCCACGCCAACTGGTACGAGCGCGAAGTGTGGGACATGTACGGCATCACCTTTCACCGGCCACCCGCACCTGACCCGCATGCTGATGCCGCCGACCTGGCAGGGCCATCCGCTGCGCAAGGACTACCCGGCGCGCGCCACCGAGTTCGATCCCTACTCCCTGTCCGCCGCCAAGCAGGACCTGGAGCAGGAAGCCCTGCGCTTCAAGCCGGAAGACTGGGGCATGAAGCGCCACGGCGAGAACGAGGACTACATGTTCCTCAACCTCGGCCCGAACCACCCGTCCGCCCACGGCGCGTTCCGCATCATCCTGCAACTGGACGGCGAGGAGATCATCGACTGCGTCCCGGAGATCGGCTACCACCACCGCGGCGCCGAGAAGATGGCCGAGCGCCAGTCCTGGCACAGTTTCATCCCCTACACCGACCGCATCGACTACCTCGGCGGGGTGATGAACAACCTGCCCTACGTACTCTCGGTGGAGAAGCTCGCCGGGATCAAGGTGCCCCAGCGGGTCGACGTGATCCGGATCATGATGGCGGAGTTCTTCCGTATCCTGAACCACCTGCTGTACCTGGGCACCTATATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACAAGGTGGTCGAGGCCATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGCGGCGTCGCCCACGACCTGCCGCGCGGCTGGGACAAGCTGGTCCGCGAGTTCCTCGACTGGATGCCCAAGCGCCTCGACGAGTACGAGACCGCGGCCCTGAAGAACAGCATCTTGCGCGGCCGGACCATCGGCGTCGCCCAGTACAACACCAAGGAAGCCCTCGAGTGGGGCACCACCGGCGCCGGCCTGCGCGCCACCGGTTGCGACTTCGACCTGCGCAAGGCCCGCCCCTACTCCGGCTACGAGAACTTCGAGTTCGAGGTACCGCTGGCCCACAACGGCGACGCCTACGACCGCTGCATGGT
Allele: 4
>ppsA-IISR6AGATGCTTANGGAGCATAAGCTNTNGNAATCCTGGNNNCCCAANCCTCCTCCCTTCTATTNNNNGACGTCGATCGTNCGATCGCGCCNNTCGCCTGAGCGACGCCGAGGTCACCGAAGCTGGCCAAGCAGGCCATGATCATCGAGAAGCACTATGGCCGCCCGATGGACATCGAATGGGCCAAGGACGGTGACGACGGCAAGCTGTACATCGTCCAGGCACGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCATGGAGCGCTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGGCGTGCCATCGGCCAGCGCATCGGTGCCGGTCCGGTCAAGGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAACCGGGTGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCAGTGATGAAGCGCGCCAGCGCCATCGTCACCAACCGCGGCGGGCGCACCTGCCACGCGGCGATCATCGCTCGCGAACTGGGCATCCCGGCGGTGGTCGGTTGCGGCAACGCCACCCAGATCCTGCAGGATGGCCAGGGGGTGACCGTTTCCTGTGCCGAAGGCGATACCGGCTTCATCTTCGAAGGCGAACTCGGTTTCGATGTGCGCAAGAACTCGGTCGACGCCATGCCCGACCTTCCGTTCAAGATCATGATGAACGTCGGCAATCCCGATCGCGCTTTCGATTTCGCCCAGTTGCCGAACGAAGGCGTGGGCCTGGCCCGCCTCGAATTCATCATCAACCGCATGATCGGCGTGCATCCCAAGGCGTTGCTGAACTTCGCCGGCCTGCCGGCGGACATCAAGGAAAGCGTGGAGAAGCGCATCGCCGGCTATCCCGATCCGGTCGGCTTCTACGTCGAGAAGCTGGTGGAGGGCATCAGCACCCTGGCCGCGGCGTTCTGGCCGAAGAACGGTCATCGTGCGCCTGTCCGACTTCAAGTCCAATGAGTACGCCACCTGATCGGCGGCAGCTCTACGAGCAGGAGAAACATAACNCTCCACCAATGAANANANNGACNANN
Allele: 15
>trpE-IISR6ATCNNNTATTCAAGNCTCAATCGTTCAAGNACGATCCAGCNACAGCGNNNNNNGCNNNGCTTCCNCCTAATCNCGNNAAATNNGNNNNGCGCCNNCAGCNGNNNNGGCGCGNGCACGGCTGAAGGCAATGAACGCATGCATCGATCGCGTATCAAACGTAACTGCACTGCATGCGGGGGCGGATCGTGCCACCTCCGTCCCGGCGGCTTGTGATGGTTTCTTCCCACTCCAGCGCCGGCACCGAGTCGGCAACGATACCGCCGCCGGCCTGCACGTGGAGTTCACCGTTCTTGATCACCGCGGTGCGGATGGCAATGGCGGTGTCCATGTTGCCGTTCCATGCCAGGTAGCCGACCGCGCCGCCGTAGACTCCACGCTTGACCGGCTCCAGCTCGTCGATGATCTCCATGGCGCGGATCTTCGGCGCGCCGGACAGCGTACCCGCCGGCAGGATCGCCCGCAGCGCGTCCATCGCGCTGAGCCCCTCGCGCAATTGCCCGGTGACGTTGGACACGATGTGCATGACGTTGGAGTAACGTTCGATCACCATTTTTTCGGTGACCTTCACCGCGCCGATATCGGACACCCGCCCCACGTCGTTGCGCCCCAGGTCGATCAGCATCAGGTGCTCGGCGATCTCCTTGGCGTCCGACAGCAGATCCTGCTCCAGCGCCAGGTCGGCCTCTTCGTTGATCCCGCGCGGACGGGTACCGGCGATCGGGCGCACCGTCACCAGGCCATCCTCGACCCGTACCAGCACCTCCGGCGAGCTGCCCACGACATGGAAGTCGCCGAAGTTGAAGAAGTACATGTAGGGCGTCGGATTGAAACAGCGCAGCGCGCGGTACAGGTCGATGGGCGCCGCCTTGAATTCGATGGACATGCGCTGCGACGGCACCACCTGCATGCAGTCGCCGGCCAGGATGTAGTCCTTGATCCTTCCTACCGCGTCTTCATAGTCCTCGCGGGTGAAGCTGGCACGAAACGCCAGCTCACGCCTTGGGGNCCCGCACANAGTGGGGGCGGGTGCCCCATCTGCGTGCCGTGAAGTTCACCGTTATAGATGANCGNTGTACGGATAGTCNTAGCTGTNTCATGTTGCCTTCAATGTCACGTGCAATCGCGCGCGTAGACCNCTCGACGGACGCCTCTCATGATATCCTGGCGGCAGTACGCTGCGCAGCGCANNGNAAGTNGNNNCATGGCTNGCCTCGGCAATGTCGGATCAG
Allele: 8
P. aeruginosa IISR6
Allele: 5
>acsA-IISR51CNNCAGGGGTTCTTGANTACCGTCCGGGCGAGTCTACTGGTGCACCGCCGACATCGGCTGGGTCACCGGCCACACCTACATCGTCTATGGCCCGCTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGCCGAACTACCCCGACGTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGCGCCGACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGACGGTTCCAGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGTGGTACTACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCACACCGAGACCGGCGCCTGCCTGATGACCCCGCTGCCGGGCGCCCACGCGATGAAGCCGGGCTCTGCAGCCAAGCCGTTCTTCGGCGTGGTACCGGCGCTGGTGGACAACCTCGGCAACCTGATCGAGGGCGCCGCCGAGGGCAACCTGGTGATCCTCGACTCCTGGCCGGGCCAGGCGCGGACCCTGTTCGGCGACCATGACCGCTTCGTCGACACCTACTTCAAGACCTTCAAGGGCATGTACTTCACCGGCGACGGCGCGCGCCGCGACGAGGACGGCTACTACTGGATCACCGGGCGGGTCGACGACGTGCTCAACGTCTCCGGCCACCGCATGGGCACCGCCGAGGTGGAAAGCGCGATGGTCGCCCACCCGAAGGTCGCCGAGGCGGCGGTGGTCGGCATGCAGCACGACATCAAGGGGCAGCA
Allele: 15
>aroE-IISR51TGNNTTNGNGGACGGATTTTGAGACGTACGTCTCAAATGCTGCCTGACAACTCAGACAGTTGCCAATTGCCGGCGCAGCGTCTCCAACACTGGCGCCGAGGCAGGACGCACGCCACGCCAGAGGAAGAATGCCTCGGCGGCCTGCTCCACCAGCATGCCCAGGCCATCCAGGGTACGCGCCGCACCGCGTTCGGCGGCCCAGCGGTTGAAGGCGGTCGGTTCCTTGGCATACATCATGTCGTAGCAAACGGTACGGCCGGGCTCGATCACGCTCTGCGCCAGCGGCGGCACGTCGCCGGCAAGACTGGCCGAGGTGCCGTTGACGACCAGGTCGAAAGGCCCTTCGACCTCGGCGAAACCGCAGCCGCGCACCGCGCCGAGATCGGCGAACCGCTCGGCCAGATCCACGGCCTTCCGCGCCGTGCGGTTGGCGATCAGCAGCTCCGCCGGGCACTCGCCGAGGAAGGGTTCGAGCACCCCGCGCACCGCACCGCCGGCGCCGAGCAGGAGAACCCGCTTGCCGCGCAGCTCGACCCCGGCGTTCGCCGTCAGGTCCCGCAGCAAGCCCGCGCCGTCGGTGTTGTCGCCGCGCAGGCGACCGTCGGCCAGGCGGATGAGGGTGTTCACCGCCCCGGCCCGGGTGGCCCGCTCGCTCAATTCGTCCACCAGACGATAGGCCTCTTCCTTGAACGGCACGGTGACATTGGCGCCCTTGCCCTGCTCGAAGAAGCGCCGGGCGAACCCGGGGAAATCGTCCAGCGGCGCCAGCTGCGCGTCATAGACCAGCGCCTCGCCGGTCTGCTCGGCGAACAGGCGGTGGATCAGCGGCGACTTGCTGTGGCCGATGGGGTTGCCGAATACGCAATAGCGGTCCATGGGTCGTTCCTTTGCCGGGGTGGGACGGGACGAGCGGACTCGTCCCCTGCGGGCGGGTCAGGGCTGGCCGGCGAGCCAATCGCGGTCGGCGAGGAAATACTCGGTCAGGCGCGCTCCTCNNNNCCGGCC
Allele: 121
>guaA-IISR51NGGNNNNNANACCCCANNGACTNNNNGGCCCTGCTGGTGTGGGTGACTCCGGGGGGGAATTTCAGCCGTGGTAGCCGCGGATCTCTGTCTNCNCGANGAGTGTGATGAGCACGGCGACAAGGTCACCGAGATGCCGGCCGGCTTCCACATCCTGGCCAGCACCCCGAGCTGCCCGATCGCCGCCATGGCCGACGATGCCCGCGCCTACTACGGCGTGCAATTCCACCCGGAAGTCACCCACACCAAGCAGGGCCTGCGCATTCTCTCGCGCTTCGTCCTCGACATCTGCGGTTGCGCCGCGCTGTGGACCCCCTCGAACATCGTCGACGACGCCATCGCCACCGTGCGCGCCCAGGTCGGTTCCTCCAAGGTCCTGCTAGGCCTCTCCGGCGGCGTGGACTCCTCGGTGGTCGCCGCGCTGCTGCACAAGGCCATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACGAAGGCGACCAGGTGATGGCCATGTTCGCCGAGAACATGGGCGTGAAGGTGATCCGCGCCAACGCCGAGGACAAGTTCCTCGGCCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGCAAGATCATCGGCCGCACCTTCATCGAAGTCTTCGACGAAGAAGCCACCAAGCTGCAGGACGTGAAGTTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCGGCCGGCGCCAAGACCGGCAAGGCCCACGTGATCAAGTCGCACCACAACGTCGGCGGCCTGCCGGAGGACATGCAGTTCGAACTGGTCGAGCCGCTGCGCGAACTGTTCAAGGACGAAGTGCGCAAGATCGGCCTGGAGCTGGGCCTGCCCTACGACATGGTCTACCGCCACCCGTTCCCCGGCCCGGGCCTGGGCGTGCGCATCCTCGGCGAGGTGAAGAAGGAGTACGCCGACCTGCTGCGCCAGGCCGACCACATCTTCATCGAGGAACTGCGCGCCTTCGACTGGTACCACAGACAGCCCGGGGGCGTTCAGTTCNATGGCTCAAAGATCTAGTAACGGCTTGCAAATTCNGCCCGGGATANTCACNCCANTACCATGCCNNNAGGCGCATTCC
Allele: 36
>mutL-IISR51NCGAGGTCATGGTNNNCGCGCAACCGAANTATCGAGGCAAGCGACTTCGCCNACGAAGCCGAGGCTTCATCACCCGCTCCATGNCNTCAATAGTCCCCCGCGTGAGTGTCGAGCGTCCCGCTCGGTGGCCAAGGAACTCTGGAAAACAGCTTGACGCCGGTTCCCGGCGCATTGATGTGGAGGTCGAGCAGGGCGGCATCAAGTTGCTGCGAGTGCGCGACGACGGTCGCGGCATCCCCGCCGACGACCTGCCGCTGGCCCTGGCTCGCCACGCCACCAGCAAGATCCGCGAGCTGGAAGACCTGGAGCGGGTGATGAGCCTCGGCTTCCGTGGCGAGGCGCTGGCCTCGATCAGTTCGGTAGCGCGCCTGACCATGACCTCGCGTACCGCCGACGCCGGCGAAGCCTGGCAGGTGGAAACCGAGGGCCGTGACATGCAGCCGCGGGTACAGCCGGCGGCGCACCCGGTGGGGACCAGCGACGAGGTTCGCGACCTGTTCTTCAACACCCCGGCGCGGCGCAAGTTCCTGCGCGCCGAGAAGACCGAGTTCGACCATCTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCACCTGCGCCACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGCGCGCGCCGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAGGTCGAGCGCAACGGCCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCCAGCCGGACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCCACGCGGTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCTGTTCTTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAGTTCGCTTCCGTGACAGCCGGATGGTCCATGACTTCCTCTANGGGGNCACCNGAA
Allele: 5
>nuoD-IISR51CCCCCGNTNGCCNACGCCACTGGTACGAGCGCGAAGTGTGGGACATGTACGGCATCACCTTCACCGGCCACCCGCACCTGACCCGCATGCTGATGCCGCCGACCTGGCAGGGCCATCCGCTGCGCAAGGACTACCCGGCGCGCGCCACCGAGTTCGATCCCTACTCCCTGTCCGCCGCCAAGCAGGACCTGGAGCAGGAAGCCCTGCGCTTCAAGCCGGAAGACTGGGGCATGAAGCGCCACGGCGAGAACGAGGACTACATGTTCCTCAACCTCGGCCCGAACCACCCGTCCGCCCACGGCGCGTTCCGCATCATCCTGCAACTGGACGGCGAGGAGATCATCGACTGCGTCCCGGAGATCGGCTACCACCACCGCGGCGCCGAGAAGATGGCCGAGCGCCAGTCCTGGCACAGTTTCATCCCCTACACCGACCGCATCGACTACCTCGGCGGGGTGATGAACAACCTGCCCTACGTACTCTCGGTGGAGAAGCTCGCCGGGATCAAGGTGCCCCAGCGGGTCGACGTGATCCGGATCATGATGGCGGAGTTCTTCCGTATCCTGAACCACCTGCTGTACCTGGGCACCTATATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACAAGGTGGTCGAGGCCATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGCGGCGTCGCCCACGACCTGCCGCGCGGCTGGGACAAGCTGGTCCGCGAGTTCCTCGACTGGATGCCCAAGCGCCTCGACGAGTACGAGACCGCGGCCCTGAAGAACAGCATCTTGCGCGGCCGGACCATCGGCGTCGCCCAGTACAACACCAAGGAAGCCCTCGAGTGGGGCACCACCGGCGCCGGCCTGCGCGCCACCGGTTGCGACTTCGACCTGCGCAAGGCCCGCCCCTACTCCCGGCTACGAGAACTTCGAGTTCGAGGTACCGCTGGCCCACACGGCGACGCTACGACGCTGCNTNNNNNN
Allele: 4
>ppsA-IISR51NCGNCAAAATGNTTCTTGCNTATNNGNNNNTTTTGNGTGCTTCCTTNNGTATGTANGACGTCGATCGTGCCGATCGCGCCCGCTTCGCCTGAGCGACGCCGAGGTCACCGAGCTGGCCCAAGCAGGCCATGATCATCGAGAAGCACTATGGCCGCCCGATGGACATCGAATGGGCCAAGGACGGTGACGACGGCAAGCTGTACATCGTCCAGGCACGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCATGGAGCGCTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGGCGTGCCATCGGCCAGCGCATCGGTGCCGGTCCGGTCAAGGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAACCGGGTGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCAGTGATGAAGCGCGCCAGCGCCATCGTCACCAACCGCGGCGGGCGCACCTGCCACGCGGCGATCATCGCTCGCGAACTGGGCATCCCGGCGGTGGTCGGTTGCGGCAACGCCACCCAGATCCTGCAGGATGGCCAGGGGGTGACCGTTTCCTGTGCCGAAGGCGATACCGGCTTCATCTTCGAAGGCGAACTCGGTCCCGATGTGCGCAAGAACTCGGTCGACGCCATGCCCGACCTTCCGTTCAAGATCATGATGAACGTCGGCAATCCCGATCGCGCTTTCGATTTCGCCCAGTTGCCGAACGAAGGCGTGGGCCTGGCCCGCCTCGAATTCATCATCAACCGCATGATCGGCGTGCATCCCAAGGCGTTGCTGAACTTCGCCGGCCTGCCGGCGGACATCAAGGAAAGCGTGGAGAAGCGCATCGCCGGCTATCCCGATCCGGTCGGCTTCTACGTCGAGAAGCTGGTGGAGGGCATCAGCACCCTGGCCGCGGCGTTCTGGCCGAAGAAGGTCATCGTGCGCCTGTCCGACTTCAAGTCCAATGAGTACGCCACCTGATCGGCGCAAGCTCTACGAGCCGGAGAAAAGAGCAACACCTNCNACCATTGNNNGCANNNCAANN
Allele: 15
P. aeruginosa IISR51
>trpE-IISR51GATACCGCCGCCGGCCTGCACGTGGAGTTCACCGTTCTTGATCACCGCGGTGCGGATGGCAATGGCGGTGTCCATGTTGCCGTTCCATGCCAGGTAGCCGACCGCGCCGCCGTAGACTCCACGCTTGACCGGCTCCAGCTCGTCGATGATCTCCATGGCGCGGATCTTCGGCGCGCCGGACAGCGTACCCGCCGGCAGGATCGCCCGCAGCGCGTCCATCGCGCTGAGCCCCTCGCGCAATTGCCCGGTGACGTTGGACACGATGTGCATGACGTTGGAGTAACGTTCGATCACCATTTTTTCGGTGACCTTCACCGCGCCGATATCGGACACCCGCCCCACGTCGTTGCGCCCCAGGTCGATCAGCATCAGGTGCTCGGCGATCTCCTTGGCGTCCGACAGCAGATCCTGCTCCAGCGCCAGGTCGGCCTCTTCGTTGATCCCGCGCGGACGGGTACCGGCGATCGGGCGCACCGTCACCAGGCCATCCTCGACCCGTACCAGCACCTCCGGCGAGCTGCCCACGACATGGAAGTCGCCGAAGTTGAAGAAGTACATGTAGGGCGTCGGATTGAAACAGCGCAGCGCGCGGTACAGGTCGATGGGCGCCGCCTTGAATTCGATGGACATGCGCTGCGACGGCACCACCTGCATGCAGTCGCCGGCCAGGATGTAGTCCTTGATCCTTCCTACCGCGTTTTCATAGTCCTCGCGGGTG
Allele:8
>acsA-IISR13TGCTATCACTCTNGGGTTTTATNGTCGCTGACCCACGAGCGGGTCTTCGACTACCGTCCGGGCGAAGTCTACTGGTGCACCGCCGACATCGGCTGGGTCACCGGCCACACCTACATCGTCTATGGCCCGCTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGCCGAACTACCCCGACGTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGCGCCGACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGACGGTTCCAGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGTGGTACTACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCAGACCGAGACCGGCGCCTGCCTGATGACCCCGCTGCCGGGCGCCCACGCGATGAAGCCGGGCTCTGCAGCCAAGCCGTTCTTCGGCGTGGTACCGGCGCTGGTGGACAACCTCGGCAACCTGATCGAGGGCGCCGCCGAGGGCAACCTGGTGATCCTCGACTCCTGGCCGGGCCAGGCGCGGACCCTGTTCGGCGACCATGACCGCTTCGTCGACACCTACTTCAAGACCTTCAAGGGCATGTACTTCACCGGCGACGGCGCGCGCCGCGACGAGGACGGCTACTACTGGATCACCGGGCGGGTCGACGACGTGCTCAACGTCTCCGGCCACCGCATAAGCACCGCCGAGGTGGAAAGCGCGATGGTCGCCCACCCGAAGGTCGCCGAGGCGGCGGTGGTCGGCATGCAGCACGACATCAAGGGGCACCCATTCTCTTTAGAGNGAGGGGGGCGTC
Allele: 15
>aroE-IISR13GGNTTNNNGGGACGGATTTGAGACGTACGTCTCAAATGCTGCCTGACAACTCAGACAGTTGCCAATCGCCGGCGCAGCGTCTCCAACACTGGCGCCGAGGCAGGACGCACGCCACGCCAGAGGAAGAATGCCTCGGCGGCCTGCTCCACCAGCATGCCCAGGCCATCCAGGGTACGCGCCGCACCGCGTTCGGCGGCCCAGCGGTTGAAGGCGGTCGGTTCCTTGGCATACATCATGTCGTAGCAAACGGTACGGCCGGGCTCGATCACGCTCTGCGCCAGCGGCGGCACGTCGCCGGCAAGACTGGCCGAGGTGCCGTTGACGACCAGGTCGAAAGGCCCTTCGACCTCGGCGAAACCGCAGCCGCGCACCGCGCCGAGATCGGCGAACCGCTCGGCCAGATCCACGGCCTTCCGCGCCGTGCGGTTGGCGATCAGCAGCTCCGCCGGGCACTCGCCGAGGAAGGGTTCGAGCACCCCGCGCACCGCACCGCCGGCGCCGAGCAGGAGAACCCGCTTGCCGCGCAGCTCGACCCCGGCGTTCGCCGTCAGGTCCCGCAGCAAGCCCGCGCCGTCGGTGTTGTCGCCGCGCAGGCGACCGTCGGCCAGGCGGATGAGGGTGTTCAACGCCCCGGCCCGGGTGGCCCGCTCGCTCAATTCGTCCACCAGACGATAGGCCTCTTCCTTGAACGGCACGGTGACATTGGCGCCCTTGCCCTGCTCGAAGAAGCGCCGGGCGAACCCGGGGAAATCGTCCAGCGGCGCCAGCTGCGCGTCATAGACCAGCGCCTCGCCGGTCTGCTCGGCGAACAGGCGGTGGATCAGCGGCGACTTGCTGTGGCCGATGGGGTTGCCGAATACGCAATAGCGGTCCATGGGTCGTTCCTTTGCCGGGGTGGGNAACGGGACGAGCGAACTCGTCCCCTGGCGGGCGGGTCAGGGCTGGCCGGCGAGCCAATCGCGGTCGGCGAGGAAATACTCGGTCAGGCGCGCTCCCCGNNNCGGGCA
Allele: 121
>mutL-IISR13NGNNGCGGTACCAGAGGTCATGTCNGCGCGNACCAACNATCNCGCCAGCGCCTCNCCANNANGCCGAGGCTCATCACCGCTTCTCAGNATCTTNGTCCGCGTGAGCTGTCGAGCGTCCCGCCTCGGTGGCCAAGGAACTCTGGAAAACAGCTTGACGCCGGTTCCCGGCGCATTGATGTGGAGGTCGAGCAGGGCGGCATCAAGTTGCTGCGAGTGCGCGACGACGGTCGCGGCATCCCCGCCGACGACCTGCCGCTGGCCCTGGCTCGCCACGCCACCAGCAAGATCCGCGAGCTGGAAGACCTGGAGCGGGTGATGAGCCTCGGCTTCCGTGGCGAGGCGCTGGCCTCGATCAGTTCGGTAGCGCGCCTGACCATGACCTCGCGTACCGCCGACGCCGGCGAAGCCTGGCAGGTGGAAACCGAGGGCCGTGACATGCAGCCGCGGGTACAGCCGGCGGCGCACCCGGTGGGGACCAGCGTCGAGGTTCGCGACCTGTTCTTCAACACCCCGGCGCGGCGCAAGTTCCTGCGCGCCGAGAAGACCGAGTTCGACCATCTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCACCTGCGCCACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGCGCGCGCCGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAGGTCGAGCGCAACGGCCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCCAGCCGGACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCCACGCGGTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCTGTTCTTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAGTTCGCTTCCGTGACAGCCGGATGGTCCATGACTTCCTCTAAGAGGAGACNCCTNNN
Allele: 5
>mutL-IISR13NGNNGCGGTACCAGAGGTCATGTCNGCGCGNACCAACNATCNCGCCAGCGCCTCNCCANNANGCCGAGGCTCATCACCGCTTCTCAGNATCTTNGTCCGCGTGAGCTGTCGAGCGTCCCGCCTCGGTGGCCAAGGAACTCTGGAAAACAGCTTGACGCCGGTTCCCGGCGCATTGATGTGGAGGTCGAGCAGGGCGGCATCAAGTTGCTGCGAGTGCGCGACGACGGTCGCGGCATCCCCGCCGACGACCTGCCGCTGGCCCTGGCTCGCCACGCCACCAGCAAGATCCGCGAGCTGGAAGACCTGGAGCGGGTGATGAGCCTCGGCTTCCGTGGCGAGGCGCTGGCCTCGATCAGTTCGGTAGCGCGCCTGACCATGACCTCGCGTACCGCCGACGCCGGCGAAGCCTGGCAGGTGGAAACCGAGGGCCGTGACATGCAGCCGCGGGTACAGCCGGCGGCGCACCCGGTGGGGACCAGCGTCGAGGTTCGCGACCTGTTCTTCAACACCCCGGCGCGGCGCAAGTTCCTGCGCGCCGAGAAGACCGAGTTCGACCATCTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCACCTGCGCCACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGCGCGCGCCGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAGGTCGAGCGCAACGGCCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCCAGCCGGACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCCACGCGGTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCTGTTCTTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAGTTCGCTTCCGTGACAGCCGGATGGTCCATGACTTCCTCTAAGAGGAGACNCCTNNN
Allele: 5
>nuoD-IISR13NANTGCCCACGCACTGGTACGAGCGCGAAGTGTGGGACATGTACGGCATCACCTTCACCGGCCACCCGCACCTGACCCGCATGCTGATGCCGCCGACCTGGCAGGGCCATCCGCTGCGCAAGGACTACCCGGCGCGCGCCACCGAGTTCGATCCCTACTCCCTGTCCGCCGCCAAGCAGGACCTGGAGCAGGAAGCCCTGCGCTTCAAGCCGGAAGACTGGGGCATGAAGCGCCACGGCGAGAACGAGGACTACATGTTCCTCAACCTCGGCCCGAACCACCCGTCCGCCCACGGCGCGTTCCGCATCATCCTGCAACTGGACGGCGAGGAGATCATCGACTGCGTCCCGGAGATCGGCTACCACCACCGCGGCGCCGAGAAGATGGCCGAGCGCCAGTCCTGGCACAGTTTCATCCCCTACACCGACCGCATCGACTACCTCGGCGGGGTGATGAACAACCTGCCCTACGTACTCTCGGTGGAGAAGCTCGCCGGGATCAAGGTGCCCCAGCGGGTCGACGTGATCCGGATCATGATGGCGGAGTTCTTCCGTATCCTGAACCACCTGCTGTACCTGGGCACCTATATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACAAGGTGGTCGAGGCCATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGCGGCGTCGCCCACGACCTGCCGCGCGGCTGGGACAAGCTGGTCCGCGAGTTCCTCGACTGGATGCCCAAGCGCCTCGACGAGTACGAGACCGCGGCCCTGAAGAACAGCATCTTGCGCGGCCGGACCATCGGCGTCGCCCAGTACAACACCAAGGAAGCCCTCGAGTGGGGCACCACCGGCGCCGGCCTGCGCGCCACCGGTTGCGACTTCGACCTGCGCAAGGCCCGCCCCTACTCCGGCTACGAGAACTTCGAGTTCGAGGTACCGCTGGCCCACACGGCGACGCTACGACGCTGCANNNNGAAN
Allele: 4
>ppsA-IISR13NTCGAGGGCCATATNCNNTCGATGATCTTGCCTNCTNNGGTCGCNNNNTNNCAGTAGTGACGTCGATCGTGCCGATCGCGCCGCTTCGCCCTGAGCGACGCCGAGGTCACCGAGCTGGCCAAGCAGGCCATGATCATCGAGAAGCACTATGGCCGCCCGATGGACATCGAATGGGCCAAGGACGGTGACGACGGCAAGCTGTACATCGTCCAGGCACGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCATGGAGCGCTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGGCGTGCCATCGGCCAGCGCATCGGTGCCGGTCCGGTCAAGGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAACCGGGTGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCAGTGATGAAGCGCGCCAGCGCCATCGTCACCAACCGCGGCGGGCGCACCTGCCACGCGGCGATCATCGCTCGCGAACTGGGCATCCCGGCGGTGGTCGGTTGCGGCAACGCCACCCAGATCCTGCAGGATGGCCAGGGGGTGACCGTTTCCTGTGCCGAAGGCGATACCGGCTTCATCTTCGAAGGCGAACTCGGTTTCGATGTGCGCAAGAACTCGGTCGACGCCATGCCCGACCTTCCGTTCAAGATCATGATGAACGTCGGCAATCCCGATCGCGCTTTCGATTTCGCCCAGTTGCCGAACGAAGGCGTGGGCCTGGCCCGCCTCGAATTCATCATCAACCGCATGATCGGCGTGCATCCCAAGGCGTTGCTGAACTTCGCCGGCCTGCCGGCGGACATCAAGGAAAGCGTGGAGAAGCGCATCGCCGGCTATCCCGATCCGGTCGGCTTCTACGTCGAGAAGCTGGTGGAGGGCATCAGCACCCTGGCCGCGGCGTTCTGGCCGAAGAAGGTCATCGTGCGCCTGTCCGACTTCAAGTCCAATGAGTACGCCAACCTGATCGGCGCAAGCTCTACGAGCCTGAGNNNNTACCTNNACCATNNCGCAGCNACCANNANNNNTTNGCAGAAAGG
ppsA: 15
>trpE-IISR13GGGGGGNGGNTNTCGCTTACCGCGAGGACTATGAAACGCGGTAGGAAGGATCAAGGACTACATCCTGGCCGGCGACTGCATGCAGGTGGTGCCGTCGCAGCGCATGTCCATCGAATTCAAGGCGGCGCCCATCGACCTGTACCGCGCGCTGCGCTGTTTCAATCCGACGCCCTACATGTACTTCTTCAACTTCGGCGACTTCCATGTCGTGGGCAGCTCGCCGGAGGTGCTGGTACGGGTCGAGGATGGCCTGGTGACGGTGCGCCCGATCGCCGGTACCCGTCCGCGCGGGATCAACGAAGAGGCCGACCTGGCGCTGGAGCAGGATCTGCTGTCGGACGCCAAGGAGATCGCCGAGCACCTGATGCTGATCGACCTGGGGCGCAACGACGTGGGGCGGGTGTCCGATATCGGCGCGGTGAAGGTCACCGAAAAAATGGTGATCGAACGTTACTCCAACGTCATGCACATCGTGTCCAACGTCACCGGGCAATTGCGCGAGGGGCTCAGCGCGATGGACGCGCTGCGGGCGATCCTGCCGGCGGGTACGCTGTCCGGCGCGCCGAAGATCCGCGCCATGGAGATCATCGACGAGCTGGAGCCGGTCAAGCGTGGAGTCTACGGCGGCGCGGTCGGCTACCTGGCATGGAACGGCAACATGGACACCGCCATTGCCATCCGCACCGCGGTGATCAAGAACGGTGAACTCCACGTGCAGGCCGGCGGCGGTATCGTTGCCGACTCGGTGCCGGCGCTGGAGTGGGAAGAACCATCAACAGGCGCCGGACCGAGTCGGCACGATCCGCCACAGCTGCAGTGGAGTTCACGGTTTGATCACGGCGTGCGAGGCATGCGTTCATGTGGCTTTCAGCGGTAGCACGCGCCNAAANNCGCTGGACGGCGCGCGNCNTAATTCNGGCGGNAAATTACGCGCCGNNNNGNC
Allele: 8
P. aeruginosa IISR13
>acsA-IISR853TATTCTGGTGTTTCGCCTCGCTGACCCACGAGCGGGTCTTCGACTACCGTCCGGGCGAAGTCTACTGGTGCACCGCCGACATCGGCTGGGTCACCGGCCACACCTACATCGTCTATGGCCCGCTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGCCGAACTACCCCGACGTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGCGCCGACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGACGGTTCCAGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGTGGTACTACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCAGACCGAGACCGGCGCCTGCCTGATGACCCCGCTGCCGGGCGCCCACGCGATGAAGCCGGGCTCCGCGGCCAAGCCGTTCTTCGGCGTGGTACCGGCACTGGTGGACAATCTCGGCAACCTGATCGAGGGCGCCGCCGAGGGCAACCTGGTGATCCTCGACTCCTGGCCGGGCCAGGCGCGGACCCTGTTCGGCGACCATGACCGCTTCGTCGACACCTACTTCAAGACCTTCAAGGGCATGTACTTCACCGGCGACGGCGCGCGCCGCGACGAGGACGGCTACTACTGGATCACCGGGCGGGTCGACGACGTGCTCAACGTCTCCGGCCACCGCATGGGCACCGCCGAGGTGGAAAGCGCGATGGTCCGCCCACCCCGAAGGTCGCCGAGGCGGCGGTGGTCGGCATGCAGCACGACATCAAGGAGGGGATTTTTTTTTTNATAAGCCNCCCTGTTAGCCNGGTAACTTNGGTGGGGCACATGGTTCACTNGTCGTCCAGCCGTGGCGGAGACTTCAGAGTCTCACCGCGTGATCTTTNTAGCCNCNCTNCGCGNCNCCGNTCAATCANGTCNNGANGTTTGANNNAGTTTCGACAAAACGCTTAGNATGCCTACAGGGACCNNN
Allele: 22
>aroE-IISR853GGAGAGGGTAAANCCATTTTGAGACGTACGTCTCAAATGCCGCCTGACAACTCAGACAGTTGCCAACTGTCGGCGCAGCGTCTCCAACACTGGCGCCGAGGCAGGACGCACGCCGCGCCAGAGGAAGAATGCCTCGGCGGCCTGCTCCACCAGCATGCCCAGGCCATCCAGGGTACGCGCCGCACCGCGTTCGGCGGCCCAGCGGTTGAAGGCAGTCGGTTCCTTGGCATACATCATGTCGTAGCAGACGGTACGGCCGGGCTCGATCACGCTCTGCGCCAGCGGCGGCACGTCGCCGGCAAGACTGGCCGAGGTGCCGTTGACGATCAGGTCGAAAGGCCCTTCGACCTCGGCGAAACCGCAGCCGTGCACCGCGCCGAGGTCGGCGAACCGCTCGGCCAGGTCCACGGCCTTCCGCGCCGTGCGGTTGGCGATCAGCAACTCCGCCGGGCACTCGCCGAGGAAGGGTTCGAGCACCCCACGCACCGCACCGCCGGCGCCGAGCAGGAGAACCCGCTTGCCGCGCAGCTCGACCCCGGCGTTCGCCGTCAGGTCCCGCAGCAGGCCGGCGCCGTCGGTGTTGTCGCCGCGCAGGCGACCGTCGGCGAGGCGGATCAGGGTGTTCACCGCCCCGGCCCGGGTGGCCCGCTCGCTCAACTCGTCCACCAGACGATAGGCCTCTTCCTTGAACGGCACGGTGACATTGGCGCCCTTGCCCTGCTCGAAGAAGCGCCGGGCGAACCCGGGGAAATCGTCCAGCGGCGCCAGTTGCGCGTCATAGACCAGCGCCTCGCCGGTCTGCTCGGCGAACAGGCGGTGGATCAGCGGCGACTTGCTGTGGCCGATGGGGTTGCCGAATACGCAATAGCGGTCCATGGGTCGTTCCTTTGCCGGAGTGGGGACGGGACGAGCGGACTCGTCCCCTGCGGGCGGGTCAGGGCTGGCCGGCGAGCCAATCGCGGTCGGCGAGGAAATACTCGGTCAGGCGCGCTCCCCNCCCNCGGNN
Allele: 5
>guaA-IISR853NGTAGTGAGNAAACCAAAGAACTGGCCNNCCTGCTGGTGNTGGGTGACTTCCGGGGGAATTCCAGCGGTCGTGGGCGCGGGATTTCTGGCCTTNNAACGTGTGATGAGCCACGGCGACAAGGTCACCGAGATGCCGGCCGGCTTCCACATCCTGGCCAGCACCCCGAGCTGCCCGATCGCCGCCATGGCCGACGATGCCCGCGCCTACTACGGCGTGCAATTCCACCCGGAAGTCACCCACACCAAGCAGGGCCTGCGCATTCTCTCGCGCTTCGTCCTCGACATCTGCGGTTGCGCCGCGCTGTGGACCCCCTCGAACATCGTCGACGACGCCATCGCCACCGTGCGCGCCCAGGTCGGTTCCTCCAAGGTCCTGCTAGGCCTCTCCGGCGGCGTGGACTCCTCGGTGGTCGCCGCGCTGCTGCACAAGGCCATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACGAAGGCGACCAGGTGATGGCCATGTTCGCCGAGAACATGGGCGTGAAGGTGATCCGCGCCAACGCCGAGGACAAGTTCCTCGGCCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGCAAGATCATCGGCCGCACCTTAATCGAAGTTTTCGACGAAGAAGCCACCAAGCTGCAGGACGTGAAGTTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCAGCCGGCGCCAAGACCGGCAAGGCCCACGTGATCAAGTCGCACCACAACGTCGGCGGCCTGCCGGAGGACATGCAGTTCGAACTGGTCGAGCCGCTGCGCGAACTGTTCAAGGACGAAGTGCGCAAGATCGGCCTGGAGCTGGGCCTGCCCTACGACATGGTCTACCGCCACCCGTTCCCCGGCCCGGGCCTGGGCGTGCGCATCCTCGGCGAGGTGAAGAAGGAGTACGCCGACCTGCTGCGCCAGGCCGACCACATCTTCATCGAGGAACTGCGCGCCTTCGACTGGTACACAGACAGCCCGGGGCGTTCAGTACCAATGCCAAGNTCTAGCTACGCCNTGCAATTCCGCNNCGTAGTCNCTCCTACACCNGCNNGNNTGCCCANTCCTTGNNNCT
Allele: 83
>mutL-IISRT853AAGTCATGGTCAGGCGGCGCAACCNGGTTATCGAGNCAAGCCGCCTCGCCACGGAAGCGGGGGCTCATCACCCGCTCCTCGTANCATCGTCCGCCGGTGAGGTGGTCGAGCGTCCCGCCTCGGTGGCCAAGGAACTCTGGAAAACAGCCTTGACGCCGGTTCCCGGCGCATTGATGTGGAGGTCGAGCAGGGCGGCATCAAGTTGCTGCGAGTGCGCGACGACGGTCGCGGCATCCCCGCCGACGACCTGCCGCTGGCCCTGGCTCGCCACGCCACCAGCAAGATCCGCGAGCTGGAAGACCTGGAGCGGGTGATGAGCCTCGGCTTCCGTGGCGAGGCGCTGGCCTCGATCAGCTCGGTAGCGCGCCTGACCATGACCTCGCGTACCGCCGACGCCGGCGAAGCCTGGCAGGTGGAAACCGAGGGCCGCGACATGCAGCCGCGGGTACAGCCGGCGGCGCATCCGGTGGGGACCAGCGACGAGGTTCGCGACCTGTTCTTCAACACCCCGGCGCGGCGCAAGTTCCTGCGTGCCGAGAAGACCGAGTTCGACCATCTGCAGGAGGTCATCAAGCGCCTGGCGCTGGCCCGCTTCGACGTGGCTTTCCACCTGCGCCACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGCGCGCGCCGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAGGTCGAGCGCAACGGTCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCCAGCCGGACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCCACGCGGTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCTGTTCTTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAGTTCGCTTCCGTGACAGCCGGATGGTCCATGACTTCCTCTATGGGAACCCTGA
Allele: 2
>nuoD-IISR853TNNAGCAGCGGTCGTAGGCGTCGCCGTTGTGGGCCAGCGGTACCTCGAACTCGAAGTTCTCGTAGCCGGAGTAGGGGCGGGCCTTGCGCAGGTCGAAGTCGCAACCGGTGGCGCGCAGGCCGGCGCCGGTGGTGCCCCACTCGAGGGCTTCCTTGGTGTTGTACTGGGCGACGCCGATGGTCCGGCCGCGCAGGATGCTGTTCTTCAGGGCCGCGGTCTCGTACTCGTCGAGGCGCTTGGGCATCCAGTCGAGGAACTCGCGGACCAGCTTGTCCCAGCCGCGCGGCAGGTCGTGGGCGACGCCGCCGATGCGGTACCAGGCCGGGTGCAGGCGGAAGCCGGTGATGGCCTCGACCACCTTGTAGGCGCGCTGGCGGTCGGGGAAGGTGAAGAACACCGGGGTCATGGCGCCGACGTCCTGGATATAGGTGCCCAGGTACAGCAGGTGGTTCAGGATACGGAAGAACTCCGCCATCATGATCCGGATCACGTCGACCCGCTGGGGCACCTTGATCCCGGCGAGCTTCTCCACCGAGAGTACGTAGGGCAGGTTGTTCATCACCCCGCCGAGGTAGTCGATGCGGTCGGTGTAGGGGATGAAACTGTGCCAGGACTGGCGCTCGGCCATCTTCTCGGCGCCGCGGTGGTGGTAGCCGATCTCCGGGACGCAGTCGATGATCTCCTCGCCGTCCAGTTGCAGGATGATGCGGAACGCGCCGTGGGCGGACGGGTGGTTCGGGCCGAGGTTGAGGAACATGTAGTCCTCGTTCTCGCCGTGGCGCTTCATGCCCCAGTCTTCCGGCTTGAAGCGCAGGGCTTCCTGCTCCAGGTCCTGCTTGGCGGCGGACAGGGAGTAGGGATCGAACTCGGTGGCGCGCGCCGGGTAGTCCTTGCGCAGCGGATGGCCCTGCCAGGTCGGCGGCATCAGCATGCGGGTCAGGTGCGGGTGGCCGGTGAAGGTGATGCCGTACATGTCCCACACTTCGCGCTCGTACAGTGGCGTGNCNNNNGGG
Allele: 4
>ppsA-IISR853CTATCGGGGCANTAGNNNNTTGGGCNGTTTTTNNNNGNCNNGTTGNTCTNCGCTNNAGGTAGTGACGTCGATCGCGCCGATCGCGCCCGCTTCGCCTGAGCGACGCCGAGGTCACCGAGCTGGCCAAGCAGGCCATGATCATCGAGAAGCACTATGGCCGCCCGATGGACATCGAATGGGCCAAGGACGGTGACGACGGCAAGCTGTACATCGTCCAGGCACGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCATGGAGCGCTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGGCGTGCCATCGGCCAGCGCATCGGTGCCGGTCCGGTCAAAGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAACCGGGTGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCAGTGATGAAGCGCGCCAGCGCCATCGTCACCAACCGCGGCGGGCGCACCTGCCACGCGGCGATCATCGCTCGCGAACTGGGCATCCCGGCGGTGGTCGGTTGCGGCAACGCCACCCAGATCCTGCAGGATGGCCAGGGGGTGACCGTTTCCTGTGCCGAAGGCGATACCGGCTTCATCTTCGAAGGCGAACTCGGTTTCGATGTGCGCAAGAACTCGGTCGACGCCATGCCCGACCTTCCGTTCAAGATCATGATGAACGTCGGCAATCCCGATCGCGCTTTCGATTTCGCCCAGTTGCCGAACGAAGGCGTGGGCCTGGCCCGCCTCGAATTCATCATCAACCGCAAGATCGGCGTGCATCCCAAGGCGTTGCTGAACTTCGCCGGCCTGCCGGCGGACATCAAGGAAAGCGTGGAGAAGCGCATCGCCGGCTATCCCGATCCGGTCGGCTTCTACGTCGAGAAGCTGGTGGAGGGCATCAGCACCCTGGCCGCGGCGTTCTGGCCGAAGAAGGTCATCGTGCGCCTGTCCGACTTCAAGTCCAATGAGTACGCCAACCTGATCGGCGCAGCTCTACACNNGNNGCCCNNACCNGCCCCNCTACTNCACNCAA
Allele: 13
>trpE-IISR853CTCACTTGTCGATATGTCCATGGCGGGATTTCGCGCGCGGAAGAGTGCCCGCTGGAAGATCGCCCGCAGCGGCGTCCACCGCGTTGAGCCCCCCGCGCAATTGCCCGGTGGACGGTCGGGCCCGATGTGCACGGCGTTGGGAGGTAACCTTCCATCANCCATTTTTTTCGTGTGACCTTTCACCGCGCCGATATCGGACACCCGCCCCACGTTCGCGGGGGCCCCCAAGGGCGTGAGCGGCGTTTCGTGCCAGCTTCACCCGCGAGGACTATGAAAACGCGGTAGGAAGGATCAAGGACTACATCCTGGCCGGCGACTGCATGCAGGTGGTGCCGTCGCAGCGCATGTCCATCGAATTCAAGGCGGCGCCCATCGACCTGTACCGCGCGCTGCGCTGTTTCAATCCGACGCCCTACATGTACTTCTTCAACTTCGGCGACTTCCATGTCGTGGGCAGCTCGCCGGAGGTGCTGGTACGGGTCGAGGATGGCCTGGTGACGGTGCGCCCGATCGCCGGTACCCGTCCGCGCGGGATCAACGAAGAGGCCGACCTGGCACTGGAGCAGGATCTGCTGTCGGACGCCAAGGAGATCGCCGAGCACCTGATGCTGATCGACCTGGGGCGCAACGACGTGGGGCGGGTGTCCGATATCGGCGCGGTGAAGGTCACCGAAAAAATGGTGATCGAACGTTACTCCAACGTCATGCACATCGTGTCCAACGTCACCGGGCAATTGCGCGAGGGGCTCAGCGCGATGGACGCGCTGCGGGCGATTCTGCCGGCGGGCACTCTATCCGGCGCGCCGAAGATCCGCGCCATGGAGATCATCGACGAGCTGGAGCCGGTCAAGCGTGGAGTCTACGGCGGCGCGGTCGGCTACCTGGCATGGAACGGCAACATGGACACCGCCATTGCCATCCGCACCGCGGTGATCAAGAACGGTGAACTCCACGTGCAGGCCGGCGGCGGTATCGTTGCCGACTCGGTGCCCGCGCTGGAGTGGGAAGAAACCATCACACCCCCCGGGACCAAATCGGCACGATTCCGCCTCGCTGCAGTGAGTTAACCGTTTGGATGACGCGTTCGGAAGGCATGGCGGTTTCATGTGCNTTCAGCNGGTNCNNCGNNNCTAAACCCGCTGGACGGNNNNNTCATNTATTCGGGGCGAANTNGGCGCNAAGAGGGCNGANAAATGCCGCNGCTTGCCNTGNNGCTCANNTGNACGAATTNNNACAAATTGANN
Allele: 7
P. aeruginosa IISR853
>acsA-GEB9NTTGTNCAAAAGAATTGCTGCCCCTTGATGGTCGTGCTGCATGCCGACCACCGCCGCCTCGGCGACCTTCGGGTGGGCGACCATCGCGCTTTCCACCTCGGCGGTGCCCATGCGGTGGCCGGAGACGTTGAGCACGTCGTCGACCCGCCCGGTGATCCAGTAGTAGCCGTCCTCGTCGCGGCGCGCGCCGTCGCCGGTGAAGTACATGCCCTTGAAGGTCTTGAAGTAGGTGTCGACGAAGCGGTCATGGTCGCCGAACAGGGTCCGCGCCTGGCCCGGCCAGGAGTCGAGGATCACCAGGTTGCCCTCGGCGGCGCCCTCGATCAGGTTGCCGAGATTGTCCACCAGTGCCGGTACCACGCCGAAGAACGGCTTGGCCGCGGAGCCCGGCTTCATCGCGTGGGCGCCCGGCAGCGGGGTCATCAGGCAGGCGCCGGTCTCGGTCTGCCACCAGGTGTCGACGATCGGGCAGCGCGACTGGCCGACGGTCTCGTAGTACCACTGCCAGGCTTCCGGGTTGATCGGCTCGCCCACCGAACCGAGCAGACGCAGGCTGGAACCGTCGGCACCGGCCACCGCCGCCTTGCCTTCGGCCATCATCGCGCGGATCGCGGTCGGCGCGGTGTAGAGGATGTTGACCTTGTGCTTGTCGATGATTTTCGCCACGCGGGTCACGTCGGGGTAGTTCGGCACGCCCTCGAACAGAATGGTGGTGGCGCCGTTGGCCAGCGGGCCATAGACGATGTAGGTGTGGCCGGTGACCCAGCCGATGTCGGCGGTGCACCAGTAGACTTCGCCCGGACGGTAGTCGAAGACCCGCTCGTGGGTCAGCGAGGTAANANCCNGAGATCGA
Allele: 22
>aroE-GEB9AGCCGGGCAGAGAGGAAGGCGCCTGACCGAGTATTTCCTCGCCGACCGCGATTGACTCGCCGGCCAGCCCTGACCAGCCCGCAGGGGACGAGTCCGCTCGTCCCGTCCCACTCCGGCAAAGGAACGACCCATGGACCGCTATTGCGTATTCGGCAACCCCATCGGCCACAGCAAGTCGCCGCTGATCCACCGCCTGTTCGCCGAGCAGACCGGCGAGGCGCTGGTCTATGACGCGCAACTGGCGCCGCTGGACGATTTCCCCGGGTTCGCCCGGCGCTTCTTCGAGCAGGGCAAGGGCGCCAATGTCACCGTGCCGTTCAAGGAAGAGGCCTATCGTCTGGTGGACGAGTTGAGCGAGCGGGCCACCCGGGCCGGGGCGGTGAACACCCTGATCCGCCTCGCCGACGGTCGCCTGCGCGGCGACAACACCGACGGCGCCGGCCTGCTGCGGGACCTGACGGCGAACGCCGGGGTCGAGCTGCGCGGCAAGCGGGTTCTCCTGCTCGGCGCCGGCGGTGCGGTGCGTGGGGTGCTCGAACCCTTCCTCGGCGAGTGCCCGGCGGAGTTGCTGATCGCCAACCGCACGGCGCGGAAGGCCGTGGACCTGGCCGAGCGGTTCGCCGACCTCGGCGCGGTGCACGGCTGCGGTTTCGCCGAGGTCGAAGGGCCTTTCGACCTGATCGTCAACGGCACCTCGGCCAGTCTTGCCGGCGACGTGCCGCCGCTGGCGCAGAGCGTGATCGAGCCCGGCCGTACCGTCTGCTACGACATGATGTATGCCAAGGAACCGACTGCCTTCAACCGCTGGGCCGCCGAACGCGGTGCGGCGCGTACCCTGGATGGCCTGGGCATGCTGGTGGAGCAGGCCGCCGAGGCATTCTTCCTCTGGCGCGGCGTGCGTCCTGCCTCGGCGCCAGTGTTGGAGACGCTGCGCCGACAGTTGGCAACTGTCTGAGTTGTCAGGCGGCATTTGAGACGTANGTNNCAATTTGGNTTCCATCTG
Allele: 5
>guaA-GEB9GNGAGCGCGAGAGAATNGCGCNNNCCTTCCTTGGTNGGGNTNNATCTCGGGGGGGGAATTGCNNGCCGTCGTAGGCGCGGGATCTGTCGGTCTCTTAAGCGGTGTGATGAGCCACGGCGACAAGGTCACCGAGATGCCGGCCGCGCTTCCACAGTCCGTGGCCAGCAGCCCCGAGCTGCCCGATCGCCGCCATGGCCGACGATGCCCGCGCCTACTACGGCGTGCAATTCCACCCGGAAGTCACCCACACCAAGCAGGGCCTGCGCATTCTCTCGCGCTTCGTCCTCGACATCTGCGGTTGCGCCGCGCTGTGGACCCCCTCGAACATCGTCGACGACGCCATCGCCACCGTGCGCGCCCAGGTCGGTTCCTCCAAGGTCCTGCTAGGCCTCTCCGGCGGCGTGGACTCCTCGGTGGTCGCCGCGCTGCTGCACAAGGCCATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACGAAGGCGACCAGGTGATGGCCATGTTCGCCGAGAACATGGGCGTGAAGGTGATCCGCGCCAACGCCGAGGACAAGTTCCTCGGCCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGCAAGATCATCGGCCGCACCTTCATCGAAGTTTTCGACGAAGAAGCCACCAAGCTGCAGGACGTGAAGTTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCAGCCGGCGCCAAGACCGGCAAGGCCCACGTGATCAAGTCGCACCACAACGTCGGCGGCCTGCCGGAGGACATGCAGTTCGAACTGGTCGAGCCGCTGCGCGAACTGTTCAAGGACGAAGTGCGCAAGATCGGCCTGGAGCTGGGCCTGCCCTACGACATGGTCTACCGCCACCCGTTCCCCGGCCCGGGCCTGGGCGTGCGCATCCTCGGCGAGGTGAAGAAGGAGTACGCCGACCTGCTGCGCCAGGCCGACCACATCTTCATCGAGGAACTGCGCGCCTTCGACTGGTACCACAAGACACCCGGGGCGTATCAGCTCCNCGATCACAACTTGTNCGGCTGGCAATTCACGCCGGAATTTCCTCATCAACTCGGGGAAGANCCNNNCCTGGTCCN
Allele: 83
>mutL-GEB9TCAGGTGNTCCCGTAGAGGGAAGGTCATGGACCATCCGGCTGTCACGGAAGCGAACTTCGTGCCTGGTCGGGTGCACGTTGACGTCCACCACCGCCGGATCGACTTCGAAGAACAGCACGAAGGTCGGATGCCGGCCGTTGTACAGCACGTCGCGATAAGCCTGGCGCACCGCGTGGGCGACCAGCTTGTCGCGCACCATGCGCCCGTTCACATAGAAGTACTGCAGGTCCGGCTGGCTGCGGGAGAAGGTCGGCAAGCCGACCCAGCCCCACAGGTGCAGACCGTTGCGCTCGACCTCGATCGGCAGCGCCTGCTCGAGGAATGCCTGGCCGCACACCGCGCCGACCCGGCGCGCGCGGGCCAGCTCGTCTCGCGCCTCGTGCAGGGCGAAGATGGTCTTGCCGTTGTGGCGCAGGTGGAAAGCCACGTCGAAGCGGGCCAGCGCCAGGCGCTTGATGACCTCCTGCAGATGGTCGAACTCGGTCTTCTCGGCACGCAGGAACTTGCGCCGCGCCGGGGTGTTGAAGAACAGGTCGCGAACCTCGACGCTGGTCCCCACCGGATGCGCCGCCGGCTGTACCCGCGGCTGCATGTCGCGGCCCTCGGTTTCCACCTGCCAGGCTTCGCCGGCGTCGGCGGTACGCGAGGTCATGGTCAGGCGCGCTACCGAGCTGATCGAGGCCAGCGCCTCGCCACGGAAGCCGAGGCTCATCACCCGCTCCAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGGCGAGCCAGGGCCAGCGGCAGGTCGTCGGCGGGGATGCCGCGACCGTCGTCGCGCACTCGCAGCAACTTGATGCCGCCCTGCTCGACCTCCACATCAATGCGCCGGGAACCGGCGTCAAGGCTGTTTTTCCAGGAGTTCCTTGGCCACCGAGGCGGGACGCTCGACCACCTCACCGGCGGGGACATCATGAGATTGGAGCGGGTGATGANCCTTGGCTTCCNGGGAGAGGCCGCTCGCCTCCGATCATCTNTTTGTNCGTCTGACCATGACCTCTCGCN
Allele: 2
>nuoD-GEB9TCTGACATGCAGCGGTCGTAGGCGTCGCCGTGTGGGCCAGCGGTACCTCGAACTCGAAGTTCTCGTAGCCGGAGTAGGGGCGGGCCTTGCGCAGGTCGAAGTCGCAACCGGTGGCGCGCAGGCCGGCGCCGGTGGTGCCCCACTCGAGGGCTTCCTTGGTGTTGTACTGGGCGACGCCGATGGTCCGGCCGCGCAGGATGCTGTTCTTCAGGGCCGCGGTCTCGTACTCGTCGAGGCGCTTGGGCATCCAGTCGAGGAACTCGCGGACCAGCTTGTCCCAGCCGCGCGGCAGGTCGTGGGCGACGCCGCCGATGCGGTACCAGGCCGGGTGCAGGCGGAAGCCGGTGATGGCCTCGACCACCTTGTAGGCGCGCTGGCGGTCGGTGAAGGTGAAGAACACCGGGGTCATGGCGCCGACGTCCTGGATATAGGTGCCCAGGTACAGCAGGTGGTTCAGGATACGGAAGAACTCCGCCATCATGATCCGGATCACGTCGACCCGCTGGGGCACCTTGATCCCGGCGAGCTTCTCCACCGAGAGTACGTAGGGCAGGTTGTTCATCACCCCGCCGAGGTAGTCGATGCGGTCGGTGTAGGGGATGAAACTGTGCCAGGACTGGCGCTCGGCCATCTTCTCGGCGCCGCGGTGGTGGTAGCCGATCTCCGGGACGCAGTCGATGATCTCCTCGCCGTCCAGTTGCAGGATGATGCGGAACGCGCCGTGGGCGGACGGGTGGTTCGGGCCGAGGTTGAGGAACATGTAGTCCTCGTTCTCGCCGTGGCGCTTCATGCCCCAGTCTTCCGGCTTGAAGCGCAGGGCTTCCTGCTCCAGGTCCTGCTTGGCGGCGGACAGGGAGTAGGGATCGAACTCGGTGGCGCGCGCCGGGTAGTCCTTGCGCAGCGGATGGCCCTGCCAGGTCGGCGGCATCAGCATGCGGGTCAGGTGCGGGTGGCCGGTGAAGGTGATGCCGTACATGTCCCACACTTCGNNCTCGTACCAGTCGCGTNGGNCAGNN
Allele: 4
>ppsA-GEB9GGACAGGCGCACGATGACCTTCTTCGGCCAGAACGCCGCGGCCAGGGTGCTGATGCCCTCCACCAGCTTCTCGACGTAGAAGCCGACCGGATCGGGATAGCCGGCGATGCGCTTCTCCACGCTTTCCTTGATGTCCGCCGGCAGGCCGGCGAAGTTCAGCAACGCCTTGGGATGCACGCCGATCATGCGGTTGATGATGAATTCGAGGCGGGCCAGGCCCACGCCTTCGTTCGGCAACTGGGCGAAATCGAAAGCGCGATCGGGATTGCCGACGTTCATCATGATCTTGAACGGAAGGTCGGGCATGGCGTCGACCGAGTTCTTGCGCACATCGAAACCGAGTTCGCCTTCGAAGATGAAGCCGGTATCGCCTTCGGCACAGGAAACGGTCACCCCCTGGCCATCCTGCAGGATCTGGGTGGCGTTGCCGCAACCGACCACCGCCGGGATGCCCAGTTCGCGAGCGATGATCGCCGCGTGGCAGGTGCGCCCGCCGCGGTTGGTGACGATGGCGCTGGCGCGCTTCATCACTGGCTCCCAGTCCGGGTCGGTCATGTCGGAGACCAGGACGTCACCCGGTTGGACCTTGTCCATTTCCGACACGTCGTTGATCACTTTGACCGGACCGGCACCGATGCGCTGGCCGATGGCACGCCCTTCCACCAGGACGGTCCCCTTCTCTTTCAGCAGGTAGCGCTCCATGACCGTGGCGCTGGCGCGGCTCTTCACGGTTTCCGGGCGTGCCTGGACGATGTACAGCTTGCCGTCGTCACCGTCCTTGGCCCATTCGATGTCCATCGGGCGGCCATAGTGCTTCTCGATGATCATGGCCTGCTTGGCCAG
Allele: 13
>trpE-GEB9ANNNTGATCGATTAANTTCCNNGNGGGATGTTCGGCGCGCAGGAAAGAGTGCCCGCCTGCAGAATTCGCCCGCAGACGCNTCCACGCGATTGAGCCCCACGCGCAATGACCCGGTGACCGTGGGCCCGATGTGCACGACGTTGGAGTAACGTTCGATCACCCATTTTTTCGGTGACCTTCACCGCGCCGAAATNGGACACCCCGCCCCACGTCGTCTGCGGCCCCCAGTCGTGAGCCGGCGTTTCGTGCCAGCTTCACCCGCGAGGACTATGAAAACGCGGTAGGAAGGATCAAGGACTACATCCTGGCCGGCGACTGCATGCAGGTGGTGCCGTCGCAGCGCATGTCCATCGAATTCAAGGCGGCGCCCATCGACCTGTACCGCGCGCTGCGCTGTTTCAATCCGACGCCCTACATGTACTTCTTCAACTTCGGCGACTTCCATGTCGTGGGCAGCTCGCCGGAGGTGCTGGTACGGGTCGAGGATGGCCTGGTGACGGTGCGCCCGATCGCCGGTACCCGTCCGCGCGGGATCAACGAAGAGGCCGACCTGGCACTGGAGCAGGATCTGCTGTCGGACGCCAAGGAGATCGCCGAGCACCTGATGCTGATCGACCTGGGGCGCAACGACGTGGGGCGGGTGTCCGATATCGGCGCGGTGAAGGTCACCGAAAAAATGGTGATCGAACGTTACTCCAACGTCATGCACATCGTGTCCAACGTCACCGGGCAATTGCGCGAGGGGCTCAGCGCGATGGACGCGCTGCGGGCGATTCTGCCGGCGGGCACTCTATCCGGCGCGCCGAAGATCCGCGCCATGGAGATCATCGACGAGCTGGAGCCGGTCAAGCGTGGAGTCTACGGCGGCGCGGTCGGCTACCTGGCATGGAACGGCAACATGGACACCGCCATTGCCATCCGCACCGCGGTGATCAAGAACGGTGAACTCCACGTGCAGGCCGGCGGCGGTATCGTTGCCGACTCGGTGCCCGCGCTGGAGTGGGAAGAAACCATCACAGCCCGCCGGGACCCGAGTCGCACGATACCGCCCCCCGGCCTGCAGTGGAAGTTCACCTTTTTGATCACCGCGATGCGGAAGCAATTGGCNNNTTCCACGTTTGNCTTTCANGNCAGTAGGCCACAGCGCCCCCTTAGAACCAGCTNGACCGCTCCNNCTTNATATAATNTCTGGGNGANATTTNCGCCGCTGANGAGTGCTCTGAAGAATCCTCATNNGACATCCGC
Allele: 7
P. aeruginosa GEB9
Allelic profile of P. aeruginosa based on sequence comparison of seven house keeping genes
Strain Acs Aro Gua Mut Nuo Pps Trp1 IISR13 15 121 36 5 4 15 82 IISR6 15 121 36 5 4 15 83 IISR51 15 121 36 5 4 15 84 IISR853 22 5 83 2 4 13 75 BP35 22 5 83 2 4 13 76 GEB9 22 5 83 2 4 13 77 PAO1 7 5 12 3 4 1 7
Population snapshot of relationship among the global P. aeruginosa-burst diagramCreated by eBurst programme which is based on a novel eBurst algorithm
IISR6 &others
BP35 & others
BP35
Dutch
Chinese Strain and
IISR51, IISR13 & IISR6
Population snapshot of relationship among the global P. aeruginosa-burst diagram Created by eBurst programme which is based on a
novel profuse algorithm
1. MLST analysis revealed that the isolates can be grouped into two
i. IISR6, IISR51 and IISR13 belong to one Sequence Type
ii. BP35, IISR853 and GEB9 belong to another Sequence Type
iii. PAO1 formed singleton in the population
2. Comparison of the allelic profile with global collection (932 isolates) revealed that the
i. IISR6, IIASR51 and IISR13 clustered with a Chinese strain (ST760) has origin in human sputum ( submitted by Yun-Song Yu Zhejiang University, PR-China, 02-06-2009)?
ii. BP35, IISR853 and GEB9 are SLV of a Dutch strain (ST 575) with unknown history (Submitted by R Van MansfieldUniversity Medical Center Utrecht, Netherlands, 14-03-2008)?
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