msc bt booklet

7/31/2019 MSc BT Booklet

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CHARUSAT 2010 Page 1 of66

ACADEMICREGULATIONS

&SYLLABUS

Faculty of Applied Sciences

Master of Science Programme(Biotechnology)


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CHAROTAR UNIVERSITY OF

SCIENCE & TECHNOLOGY

Education Campus Changa, (ECC), hitherto a conglomerate of institutes of professional

education in Engineering, Pharmacy, Computer Applications, Management, Applied

Sciences, Physiotherapy and Nursing, is one of the choicest destinations by students. It

has been transformed into Charotar University of Science and Technology

(CHARUSAT) through an Act by Government of Gujarat. CHARUSAT is permitted to

grant degrees under Section-22 of UGC- Govt. of India.

The journey of CHARUSAT started in the year 2000, with only 240 Students, 4

Programmes, one Institute and an investment of about Rs. 3 Crores (INR 30 million). At

present there are seven different institutes falling under ambit of six different faculties.

The programmes offered by these faculties range from undergraduate (UG) to Ph.D

degrees including M.Phil. These faculties, in all offer 23 different programmes. A quick

glimpse in as under:

Faculty Institute ProgrammesOffered

Faculty of Technology &Engineering

Charotar Institute of Technology B.Tech

M.Tech

Ph.DFaculty of Pharmacy Ramanbhai Patel College of

PharmacyB.Pharm

M.Pharm

Ph.DFaculty of Management Studies Indukaka Ipcowala Institute of

Management

M.B.A

PGDBM*Ph.D

Faculty of ComputerApplications

Charotar Institute of ComputerApplications

M.C.A

Ph.DFaculty of Applied Sciences P.D.Patel Institute of Applied

SciencesM.Sc

M.Phil

Ph.DFaculty of Medical Sciences Charotar Institute of Physiotherapy

Charotar Institute of Nursing

B.PT

B.Sc (Nursing)

* To be started from this academic year


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The development and growth of the institutes have already led to an investment of over

Rs.63 crores (INR 630 Million). The future outlay is planned with an estimate of Rs. 250

Crores (INR 2500 Million).

The University is characterized by state-of-the-art infrastructural facilities, innovative

teaching methods and highly learned faculty members. The University Campus sprawls

over 100 acres of land and is Wi-Fi enabled. It is also recognized as the Greenest Campus

of Gujarat.

CHARUSAT is privileged to have 300 core faculty members, educated and trained in

Stanford, IITs, IIMs and leading Indian Universities, and with long exposure to industry.

It is also proud of its past students who are employed in prestigious national and

multinational corporations.

From one college to the level of a forward-looking University, CHARUSAT has the vision

of entering the club of premier Universities initially in the country and then globally.

High Moral Values like Honesty, Integrity and Transparency which have been the

foundation of ECC continue to anchor the functioning of CHARUSAT. Banking on the

world class infrastructure and highly qualified and competent faculty, the University is

expected to be catapulted into top 20 Universities in the coming five years. In order to

align with the global requirements, the University has collaborated with internationally

reputed organizations like Pennsylvania State University USA, University at Alabama at

Birmingham USA, Northwick Park Institute UK, ISRO, BARC, etc.

CHARUSAT has designed curricula for all its programmes in line with the current

international practices and emerging requirements. Industrial Visits, Study Tours, Expert

Lectures and Interactive IT enabled Teaching Practice form an integral part of the unique

CHARUSAT pedagogy.


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The programmes are credit-based and have continuous evaluation as an important feature.

The pedagogy is student-centred, augurs well for self-learning and motivation for enquiry

and research, and contains innumerable unique features like:

Participatory and interactive discussion-based classes. Sessions by visiting faculty members drawn from leading academic institutions

and industry.

Regular weekly seminars. Distinguished lecture series. Practical, field-based projects and assignments. Summer training in leading organizations under faculty supervision in relevant

programmes.

Industrial tours and visits. Extensive use of technology for learning. Final Placement through campus interviews.

Exploration in the field of knowledge through research and development and

comprehensive industrial linkages will be a hallmark of the University, which will mould

the students for global assignments through technology-based knowledge and critical

skills.

The evaluation of the student is based on grading system. A student has to pursue his/her

programme with diligence for scoring a good Cumulative Grade Point Average (CGPA)

and for succeeding in the chosen profession and life.


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CHAROTAR UNIVERSITY OF SCIENCE AND TECHNOLOGY

Faculty of Applied Sciences

ACADEMIC REGULATIONSM. Sc. (Biotechnology) Programme

Charotar University of Science and Technology (CHARUSAT)CHARUSAT Campus, At Post: Changa 388421, Taluka: Petlad, District: Anand

Phone: 02697-247500, Fax: 02697-247100, Email: [email protected]

Year 2010


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CHARUSAT

FACULTY OF APPLIED SCIENCES

ACADEMIC REGULATIONSM. Sc. (Biotechnology) Programme

To ensure uniform system of education, duration of post graduate programmes,

eligibility criteria for and mode of admission, credit load requirement and its

distribution between course and system of examination and other related aspects,

following are the academic rules and regulations.

1. System of EducationThe Semester system of education shall be followed across The Charotar University of

Science and Technology (CHARUSAT) at Masters levels. Each semester will be at

least 90 working day duration. Every enrolled student will be required to do a

specified course work in the chosen subject of specialization and also complete a

project/dissertation if any. Medium of instruction will be English

2. Duration of ProgrammePostgraduate programme (M.Sc.)

Minimum 4 semesters (2 academic years)

Maximum 6 semesters (3 academic years)

The maximum limit can be extended by 1 or 2 semester subject to the

approval of university on case to case basis.

3. Eligibility for admissionsFor the admission to M.Sc., programs in the subject of

Biological/Physical/Mathematical/Chemical Sciences a candidate must have

obtained a Degree of Bachelor of Science from any recognized University or aDegree recognized as equivalent thereto, with minimum Second Class.

4. Mode of admissionsAdmission to M.Sc. programme will purely on combined merit of admission test

and performance at graduation.


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5. Programme structure and CreditsA student admitted to a program should study the course and earn credits

specified in the course structure. (Please refer Annexure-A)

6. Attendance6.1All activities prescribed under these regulations and listed by the course faculty

members in their respective course outlines are compulsory for all students

pursuing the courses. No exemption will be given to any student from attendance

except on account of serious personal illness or accident or family calamity that

may genuinely prevent a student from attending a particular session or a few

sessions. However, such unexpected absence from classes and other activities will

be required to be condoned by the Dean/Principal.

6.2Student attendance in a course should be 80%.

7. Course Evaluation7.1The performance of every student in each course will be evaluated as follows:

7.1.1 Internal evaluation by the course faculty member(s) based on continuousassessment, for 30% of the marks for the course; and

7.1.2 Final examination will be conducted by the University t for 70% of themarks for the course.

7.2Internal Evaluation7.2.1 Internal evaluation will be based on internal tests and several other tools of

assessment like, quiz, viva, seminar etc., as prescribed by concerned

teacher and decided by the faculty.

7.3Internal Institutional evaluation for practicals7.3.1 One internal practical test/viva will be conducted per semester totaling to

30 % internal marks for practicals

7.3.2 In Continuous evaluation Students shall be evaluated in a continuousmanner for their involvement in the practical, aptitude for learning,completion of practical related assignments, regularity in the practicals and

record keeping

7.4 University Examination7.4.1 The final examination by the University for 70% of the evaluation for the

course will be through written paper or practical test or oral test or

presentation by the student or a combination of any two or more of these.

7.4.2 In order to earn the credit in a course a student has to obtain grade otherthan FF.

7.5 Performance at Internal & University Examination


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7.5.1 Minimum performance with respect to internal marks as well as universityexamination will be an important consideration for passing a course.Details of minimum percentage of marks to be obtained in theexaminations are as follows

Minimum marks in University

Exam per subject

Minimum marks

Overall per subject

40% 50%

7.5.1. If a candidate obtains minimum required marks per subject but fails toobtain minimum required overall marks, he/she has to repeat the university

examination till the minimum required overall marks are obtained.(As per

the clause 8.2(iv)

8 Grading8.1 The internal evaluation marks and final University examination marks in

each course will be converted to a letter grade on a ten-point scale as per the following

scheme:

Grading Scheme:

Range of Marks

(%)

80 75


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9. Awards of Degree9.1 Every student of the programme who fulfils the following criteria will be

eligible for the award of the degree:

9.1.1 He should have earned at least minimum required credits as prescribed in

course structure; and

9.1.2 He should have cleared all internal and external evaluation components in

every course; and

9.1.3 He should have secured a minimum CGPA of 5.0 at the end of theprogramme;

9.1.4 In addition to above, the student has to complete the required formalities as

per the regulatory bodies.

9.2 The student who fails to satisfy minimum requirement of CGPA will be

allowed to improve the grades so as to secure a minimum CGPA for award ofdegree. Only latest grade will be considered.

10 Award of Class:

The class awarded to a student in the programme is decided by the final CGPA as

per the following scheme:

Distinction: CGPA 7.5

First class: CGPA 6.0

Second Class: CGPA 5.0

11 Transcript:The transcript issued to the student at the time of leaving the University will

contain a consolidated record of all the courses taken, credits earned, grades

obtained, SGPA,CGPA, class obtained, etc.


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ANTEXURE-A

CHAROTAR UNIVERSITY OF SCIENCE & TECHNOLOGY

M.Sc. (Biotechnology) Programme

Sem Subject

Code

Subjects Teaching scheme Theory Evaluation Practical Evaluation Total

I +II

L P Contact

hrs.

Total

Credits

Institute University Total-I Institute University Total-II

I

BT701 General microbiology 3 3 6 5 30 70 100 15 35 50 150BT702 Cell biology 3 3 6 5 30 70 100 15 35 50 150BT703 Bioinstrumentation and

biostatistics3 3 6 5 30 70 100 15 35 50 150

BT704 Molecular biology 3 3 6 5 30 70 100 15 35 50 150CS703 Communication Skills 2 2 4 3 15 35 50 30 70 100 150AP701 Seminar/Assignment/Mini-

projects/library8 4

Sem Subject

Code


I +II

L P Contact

hrs.

Total

Credits


II

BT705 Immunology 3 - 3 3 30 70 100 - - - 100

BT706 Genetics and biochemistry 3 - 3 3 30 70 100 - - - 100BT707 Bioprocess technology 3 - 3 3 30 70 100 - - - 100BT708 Industrial biotechnology 3 - 3 3 30 70 100 - - - 100BT709 Biotechnology Laboratory I - 12 12 8 60 140 200BT710 Computational Skills 2 2 4 3 15 35 50 30 70 100 150AP702 Seminar/Assignment/Mini-

projects/library8 4


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M.Sc. (Biotechnology) Programme

Sem Subject

Code


I+II

L P Contact

hrs.

Total

Credits


III

BT801 Advanced genetics and GeneticEngineering

3 3 3 30 70 100 - - - 100

BT802 Plant and Animal

biotechnology3 3 3 30 70 100 - - - 100

BT803 Environment Biotechnology

and bioinformatics3 3 3 30 70 100 - -- 100

BT804

Electives group

1. Advanced Bioprocess

Technology andBiopharmaceuticals

2. Molecular Medicine

3 3 3 30 70 100 - - - 100

BT805 Biotechnology Laboratory II 12 12 8 - - - 60 140 200 200BT806 Skills for Biological Sciences I 3 3 2 45 105 150 150AP801 Seminar/Assignment/Mini-

projects/library9 5

Sem Subject

Code


I+II

L P Contact

hrs.

Total Credits Institute University Total-

I

Institute University Total-

II

IV

BT807 Dissertation 08 25 32 24 100 150 250 150 200 350 600BT808 Skills for Biological Sciences II 01 02 04 03 15 35 50 30 70 100 150

In every programme additional credits can be earned by candidates by undertaking summer training projects:

1 Credits/project (Minimum 15 working days)

Minimum credits to earn a degree is 25x4 = 100


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M. Sc. (Biotechnology) Programme

SYLLABI(Semester 1)



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BT 701: GENERAL MICROBIOLOGY

Credit: 27 Semester - 1

A. Objective of the course. Understanding the diverse groups of microorganisms will help student to appreciate

the nature and play a positive role in solving global environmental problems. Recognize and describe the structure and function of prokaryotic cells including

differences between Gram positive and negative bacterial cell structure and to defendthis information in class discussions and on exams.

Demonstrate proper use and care of microscope and other delicate lab instruments asassessed during routine laboratory exercise.

Explain and demonstrate the importance of aseptic techniques as evaluated duringroutine laboratory exercises and applying universal precautions in hospital and homesetting.

To evaluate and describe various physical and chemical methods and how they can beused to control microbial growth.

To explain and defend in exams the mechanism of microbial growth, microbialmetabolism and genetics.

Distinguish between various disease causing microbes, recognize how to effectivelytreat them and apply this information in laboratory discussion and in exams.

B. Outline of the CourseSr. No Title of the units Minimum

number of hoursBT 701 - GENERAL MICROBIOLOGY

1 Introduction to microorganisms and methods oftheir study

9h

2 Molecular evolution and microbial systematics 9h3 Diversity of microorganisms- I 9h4 Diversity of microorganisms- II 9h5 Microbial nutrition and growth 9h


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C. Detailed syllabus.

Sr. No Title of the units Minimumnumber of

hours

Weightage

BT 701 - GENERAL MICROBIOLOGY

Unit 1 Introduction to microorganisms and methods oftheir study

9h 20%

Introduction to microbiology and brief history, thenature of the microbial world, diverse groups ofprokaryotic and eukaryotic microorganisms,microscopy, stains and staining techniques,sterilization, control of microorganisms, isolation,

cultivation and preservation of microorganisms,safety in the microbiological laboratory

Unit 2 Molecular evolution and microbial systematics 9h 20%

Early earth, origin of biological molecules, theevolutionary time scale- eras, periods and epoch,major events in the evolutionary time scale, thefirst cell, evolution of prokaryotes and eukaryotes,spontaneity of mutations, evolutionary change in

nucleotide sequences, molecular divergence,molecular clocks, origin of new genes and proteins,evolution by gene duplication, domain shuffling,transposition and horizontal gene transfer,concept of microbial species, theories and methodsof microbial systematics, nomenclature, methodsof determining evolutionary relationships,phylogenetic relationships of microorganisms,Bergeys Manual of Systematic Bacteriology

Unit 3 Diversity of microorganisms- I 9h 20%Principles of microbial diversity- distribution,

abundance and ecological niche, microbialdiversification, morphological, cultural, molecularand genomic methods for study of microbialdiversity, salient features of major groups ofDomain Prokaryotes and Archea, exploitation ofmicrobial diversity, viral diversity


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Unit 4 Diversity of microorganisms- II 9h 20%General characteristics, diversity, classificationand economic importance of cyanobacteria, algae

and fungi, salient features of Chytridiomycota,Mastigomycota, Zygomycota, Ascomycota, Basidiomycotaand Deuteromycota, identification and generalcultivation methods for economically importantcyanobacteria, fungi and algae

Unit 5 Microbial nutrition and growth 9h 20%Principles of microbial nutrition, nutritionalcategories of microorganisms, culture media forcultivation of microorganisms, batch, fed-batchand continuous growth, synchronous growth,

mathematical nature and expression of microbialgrowth, measurement of microbial growth,microbial growth curve, attached growth andbiofilms

D. Instructional Methods and Pedagogy:

The topics will be discussed in interactive class room sessions using classical black-board

teaching to power-point presentations. Practical sessions will be conducted in a highly

equipped laboratory. Experiments will be carried out by students individually. Special

interactive problem solving sessions will be also conducted by respective faculty members

on weekly bases. Course materials will be provided to the students from various primary

and secondary sources of information. Unit tests will be conducted regularly as a part of

continuous evaluation and suggestions will be given to student in order to improve their

performance.


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E. Student learning outcome/objective

By the end of this course, students will be able to:

Describe various theoretical models of the creative process. Apply a variety of creativity enhancing modes in a team setting. Understanding their own personal style of learning and how this relates to

leadership.

Identify and interact with creative pockets in the community to continue to re-energize their skills.

F. Recommended Study Material:

Reference books:

1. Analytical Microbiology Edt by Frederick Kavanagh Volume I & II. AcademicPress New York

2. Applied Microbial Physiology by Rhodes.3. Bergey's Manual of Determinative Bacteriology : 9th Edition4. Biochemical Engineering Fundamentals by Bailey and Ollis, Tata McGraw Hill,

N.Y

5. Brock the biology of microorganisms. Tata McGraw Hill, N.Y6. Experimental Microbial Ecology by Aronson, Academic Press7. Extreme Environment. Mechanism of Microbial Adaptation. Edited by Milton R.

Heinrich. Academic Press

8. Extremophiles by Johri B.N. 2000. Springer Verlag. , New York9. Introductory Practical Microbiology, by Mu dili, J (2007) Narosa Publ. House Pvt.

Ltd., New Delhi (ISBN: 978-81-7319-744-4)

10. Microbial Ecology. 2nd Edition. by R. Campbell. Blackwell Scientific Publication.11. MIcrobial Life in Extreme Environments. Edited by D. J. Kushner. Academic Press12. Microbial Physiology and Metabolism by Caldwell D.R. 1995, Brown Publishers.13. Microbial Physiology by Benjamin14. Microbial Physiology by Moat A.G. and Foster J. W. 1999.. Wiley.


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15. Microbiology 5 th edition by Pelczar M.J.Jr. Chan E.C.S., Kreig (2006) TataMcGraw Hill.

16. Microbiology and Biotechnology: A Laboratory Manual, by Kalaichelvan, PT(2006) MJP Publ., Chennai (ISBN:81-8094-008-X)

17. Microbiology of Extreme Environments and its potential for Biotechnology. Editedby M.S. Da Costa, J.C. Duarate, R.A. D. Williams. Elsiever Applied Science,

London.

18. Microbiology of Soil by Alexander and Martin19. Microbiology Principles and Explorations by Black, J.G. (2005) 6 th edition John

Wiley and Sons Inc.

20.Microbiology: Dynamics and Diversity by Perry.21. Microbiology-A Laboratory Manual, by Cappucino, J and Sherman, N.C. ( ) The

Benjamin-Cummings Publ. Co., Inc

22.Principles of Microbiology by R.MAtlas23.Microbiology by Willey,Sherwood,WoolvertonTata McGraw Hill.24.Source Book for Experiments for the Teaching of Microbiology, by Primrose, SB

and Wardlow, AC (1982) Academic Press, London (ISBN: 0-12-565680-7).

Microbial diversity by Colwd. D


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BT702 - CELL BIOLOGY


A. Objective of the course. Describe the structure and functions of biological molecules Differentiate between prokaryotes and eukaryotes, and their cellular components Clarify cell organelles, surface structures and their functions Explain mitosis and meiosis Awareness against cancer and cancer causing agents.

B. Outline of the Course

Sr.No Title of the units Minimum noof hours

1 Introduction to the Cell,Cellular Organization and

Function

9h

2 Structural Organization andFunction of Intracellular

Organelles

9h

3 Cell Signaling 9h4 Cellular Communication 9h5 Cell Division and Cell Cycle 9h


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C. Detailed syllabusSr. No. Title of the units Min of

hours

Unit 1 INTRODUCTION TO THE CELL, CELLULARORGANIZATION AND FUNCTION 9h 20%

Evolution and history of the cell, structure andorganization of prokaryotic and eukaryotic cells,shape and size of cells, molecular organization andfunctions of biomembranes, cell permeability,transport across membranes, passive diffusion,facilitated diffusion, osmosis, ion channels, ionpumps, active transport and receptor mediatedendocytosis, exocytosis, mechanism of sorting andregulation of intracellular transport, membrane

excitability, electrical properties of membranes,techniques and methods used to cellsUnit 2 STRUCTURAL ORGANIZATION AND

FUNCTION OF INTRACELLULARORGANELLES

9h 20%

Structure and functions of cell wall, nucleus,mitochondria, golgy bodies, lysosomes, endoplasmicreticulum, peroxisomes, plastids, vacuoles,chloroplast, cytoskeleton and its role in motility,special cellular structures in prokaryotes, cellmovement, vesicular transport and membrane

transport.Unit 3 CELL SINGNALING 9h 20%

Hormones and their receptors, cell surface receptor,characteristics of receptors, signaling through G-protein coupled receptors, signal transductionpathways, secondary messengers, regulation ofsignaling pathways, bacterial and plant twocomponent signaling systems, bacterial chemotaxisand quorum sensing

Unit 4 CELLULAR COMMUNICATION 9h 20%Regulation of hematopoeisis, general principles of

cell communication, cell adhesion and roles ofdifferent adhesion molecules, gap junctions,extracellular matrix, integrins, neurotransmissionand its regulation

Unit 5 CELL DIVISION AND CELL CYCLE 9h 20%Mitosis and meiosis, regulation of mitosis andmeiosis, cell cycle and its control, cell death,introduction to cancer


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D. Instructional Methods and Pedagogy:The topics will be discussed in interactive class room sessions using classical black-boardteaching to power-point presentations. Practical sessions will be conducted in a highlyequipped laboratory. Experiments will be carried out by students individually. Specialinteractive problem solving sessions will be also conducted by respective faculty memberson weekly bases. Course materials will be provided to the students from various primaryand secondary sources of information. Unit tests will be conducted regularly as a part ofcontinuous evaluation and suggestions will be given to student in order to improve theirperformance.

E. Student learning outcome/objectiveBy the end of this course, students will be able to: Describe various theoretical models of the creative process.

Apply a variety of creativity enhancing modes in a team setting. Understanding in their own style of learning and how this relates to leadership. Identify and interact with creative pockets in the community to continue to re-

energize their skills.

F. Recommended Study Material:1. Essentials of Cell and Molecular Biology: by de Robertis E. D. P. and E. M. F. ,

Holt Saunder's International Edition (new edition)

2. Essentials of Molecular Biology, 4th edn., by Malacinski GM (2003) Jones &Batiett, London. (ISBN: 0-7637- 2133-6)

3. Instant Notes : Immunology, by Ladyard, Whelan and Fanger (new edition), Vivabooks

4. Molecular Biotechnology Glick5. The Physiology and Biochemistry of Prokaryotes, by White, D (2000) Oxford

University Press, Oxford

6. The Biochemistry of Cell Signalling- Ernst J. M. Helmreich ( Indian Edition)-2005Oxford University Press

7. Molecular biology of the Cell by Bruce Alberts.8. Molecular biology of the Cell by Lodish .9. Immunology by kuby.willey and sons.10. Immunobiology by Janeway.11. The Microbial Cell Cycle. Gloover & Hopwood


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BT703 -BIOINSTRUMENTATION AND BIOSTATISTICS


A. Objective of the course. This course is designed to cover the tools and techniques of modern statistics with

specific applications to biomedical and clinical research. Both parametric and

nonparametric analysis will be presented. Descriptive statistics will be discussed

although emphasis is on inferential statistics and experimental design.

The objective of the course is to equip students with research skills necessary for asuccessful career in biomedical engineering. With this in mind, the course has

been structured to provide students experience at: i) applying engineering tools

and concepts learned in class and in other courses to realistic biological problems,

ii) working in different teams to complete research projects, and iii) collecting and

presenting data in a meaningful and professional manner

B.

Outline of the Course


1 Microscopic, Histochemical,Immunotechniques AndImaging Techniques

9h

2 Biophysical Methods- I 9h3 Biophysical Methods- Ii 9h4 Molecular Biology Methods 9h

5 Biostatistics 9h


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Sr.No Title of the units Min ofhours

Unit 1 MICROSCOPIC, HISTOCHEMICAL,IMMUNOTECHNIQUES AND IMAGINGTECHNIQUES

9h 20%

Principle and applications of light, phase contrast,fluorescent microscopy, scanning electronmicroscopy (SEM), transmission electronmicroscopy (TEM), scanning tunneling microscopy(STM), atomic force microscopy (AFM), confocal

microscopy, cytophotometry and flow cytometry,ELISA, RIA, western blot, immunoprecipitation, insitu localization in cells by FISH and GISH, imagingtechniques- plasma emission tomography (PET),MRI, FMRI, CAT

Unit 2 BIOPHYSICAL METHODS- I 9h 20%UV/Vis, fluorescence, circular dichroism (CD),NMR, ESR, X-ray diffraction, light scattering, massspectrometry, centrifugation, atomic absorptionspectroscopy, surface plasma emission spectroscopy,IR and Raman spectroscopy, single neutron

recording, patch clamp recording, ECGUnit 3 BIOPHYSICAL METHODS- II 9h 20%

Chromatography, electrophoresis, measurement ofviscosity, osmosis and pH, electrochemicaltechniques, radioisotope techniques, safetyguidelines for use of radioisotopes, tracer techniquesin biological research

Unit 4 MOLECULAR BIOLOGY METHODS 9h 20%Isolation, purification and analysis of RNA, DNA(genomic and plasmid), proteins, isoelectric focusing(IEF), PCR, detection of post translationalmodification of proteins, DNA sequencing methods,methods for analysis of gene expression at RNA andprotein level, isolation, separation and analysis oflipid molecules, microarrays, SAGE, RFLP, RAPDand AFLP techniques, protein sequencing


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Unit 5 BIOSTATISTICS 9h 20%Populations, samples and inference, variables, datarepresentation, measures of central tendency anddispersion, probability distributions (binomial,poisson and normal), sampling distribution,hypothesis testing, confidence interval, analysis ofvariance (ANOVA), regression and correlation, t-

test, F-test, 2 test, introduction to experimentaldesigns .

D. Instructional Methods and Pedagogy:The topics will be discussed in interactive class room sessions using classical black-board

teaching to power-point presentations. Practical sessions will be conducted in a highlyequipped laboratory. Experiments will be carried out by students individually. Specialinteractive problem solving sessions will be also conducted by respective faculty memberson weekly bases. Course materials will be provided to the students from various primaryand secondary sources of information. Unit tests will be conducted regularly as a part ofcontinuous evaluation and suggestions will be given to student in order to improve theirperformance.

E. Student learning outcome/objectiveBy the end of this course, students will be able to:

1. Describe various theoretical models of the creative process.2. Apply a variety of creativity enhancing modes in a team setting.3. Understanding in their own style of learning and how this relates to leadership.4. Identify and interact with creative pockets in the community to continue to re-


F. Recommended Study Material:1. An Introduction to Genetic Engineering By Desmond S. T. Nicholl2. Biochemical Calculations by Segel. I. R. - 1995 - John Wiley and Sons.3. From Genes to Genomes: Concepts and Applications of DNA Technology by

Jeremy W. Dale (2007).

4. Gene and Genome Technology: Principles and Applications of Recombinant DNAand Genomics by Sandy Primrose

5. Methods in Biotechnology, by Schmauder, H.P, Schweizer, M and Schewizer, L.M(2003), Taylor and Francis Ltd., London (ISBN: 0-7484-0430-9)


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6. Microbial Genomes - D. A. Realman and E. Strauss - 2000 - American Academy OfMicrobiology (http:// WWW. ASMUSA.ORG / ACASRC / ACA1 .HTM

7. Molecular Biotechnology Glick8. Spectrometric Identification of Organic compounds by R M Silverstein and F X

Webster, (2002)

9. Spectroscopy: D.R.Browning10. Validation Standard Operating Procedures, 2nd edn., by Haider, SI (2006) CRC

Press Taylor and Francis Group, NY (ISBN: 0-8493-9529

11. Elements of biostatistics by S. Prasad. C. Edwards12. Genetics as a tool in Microbiology13. Biostatistics by Lewis A.E.14. Statistics and experimental design by G. M. Clarke15. Gene cloning and manipulation Christopher Howe16. Basic Biotechnology by Colin Ratledge and Bjor17. Methods in Biotechnology by Hans-peter-schmauder18. Recombinant DNA Principles and Methodology by James J Greene & Venigalla B.

Rao

19. Molecular Bio methods Handbook by Rapley & Walker20. Analytical biochemistry by Wilson and walker.21. Biochemical Methods by Pingoud A. etl.


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BT704 - MOLECULAR BIOLOGY


A. Objective of the course To gain a thorough understanding of the basic principles of molecular biology.

To understand the tools of DNA technology. To be able to read and interpret

scientific papers. To design approaches to addressing questions in molecular

biology and to interpret experimental data in molecular biology.

To become proficient with a number of advanced and basic tools in molecularbiology. To interpret and design experiments.

To keep a laboratory notebook, to gain the confidence and skills necessary tobe able to attempt new laboratory procedures and troubleshoot their

implementation. To be competitive for employment in an introductory

laboratory research position.



1 Nucleic Acid Structure andGenome Organization

9h

2 DNA Replication,Recomibination and Repair

9h

3 RNA Synthesis andProcessing

9h

4 Protein Synthesis andProcessing

9h

5 Control of Gene Expression 9h


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C. Detailed syllabusSr.No Title of the units Min of

hours

Unit 1 NUCLEIC ACID STRUCTURE AND GENOMEORGANIZATION 9h 20%

DNA as a genetic material, DNA structure, DNAtopology, supercoiling, topoisomerases I and II, C-value paradox, structure of mRNA, rRNA and tRNA;packaging in viruses, prokaryotes and eukaryotes,structure of chromatin and chromosomes, DNA-protein interactions, interrupted genes, genefamilies, unique and repetitive DNA, transposons

Unit 2 DNA REPLICATION, RECOMIBINATION ANDREPAIR

9h 20%

DNA replication in DNA viruses, prokaryotes andeukaryotes; enzymes involved and mechanism ofreplication, replication models, regulation ofreplication, role of telomerases, DNA synthesis inretroviruses, mechanism of recombination andrepair, inhibitors of replication, extrachromosomalreplicons

Unit 3 RNA SYNTHESIS AND PROCESSING 9h 20%Organization of transcriptional units, transcriptionin prokaryotes and eukaryotes, transcription factors,transcription activators and repressors, RNA

polymerases, formation of initiation complex and itsregulation, RNA processing, RNA editing, splicing,post transcriptional modifications, RNA transport,catalytic RNA

Unit 4 PROTEIN SYNTHESIS AND PROCESSING 9h 20%Ribosomes, genetic code, initiation, initiation factorsand their regulation, elongation and elongationfactors, translational proof reading, posttranslational modification of proteins, translationalinhibitors, role of tRNA, aminoacylation of tRNA,aminoacyl tRNA synthetase

Unit 5 CONTROL OF GENE EXPRESSION 9h 20%

Regulation of phages (T4, T7 and ), plant andanimal viruses; operon concept, regulation ofprokaryotic and eukaryotic gene expression,chromatin remodeling, regulation of lac, ara, his andtrp operons, gene silencing, regulatory circuits, roleof chromatin in gene expression


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The topics will be discussed in interactive class room sessions using classical black-boardteaching to power-point presentations. Practical sessions will be conducted in a highlyequipped laboratory. Experiments will be carried out by students individually. Specialinteractive problem solving sessions will be also conducted by respective faculty memberson weekly bases. Course materials will be provided to the students from various primaryand secondary sources of information. Unit tests will be conducted regularly as a part ofcontinuous evaluation and suggestions will be given to student in order to improve theirperformance.

E. Student learning outcome/objectiveBy the end of this course, students will be able to:

Describe various theoretical models of the creative process. Apply a variety of creativity enhancing modes in a team setting. Understanding their own personal style of learning and how this relates to

leadership. Identify and interact with creative pockets in the community to continue to re-


F. Recommended Study Material:Reference books:

1. An introduction to human molecular genetics: mechanisms of inherited diseases2. Jack j. Pasternak 2000.3. Computational biology and genome informatics jason t. L. Wang , cathy 2003

world scientific

4. Genome transcriptome and proteome analysis by alain bernot, james2004 johnwiley and sons

5. Methods in biotechnology and bioengineeringby s. P. Vyas, d.2002cbs publishers .6. Molecular genetics of bacteria jeremy dale, simon f 2004 john wiley and sons.7. Practical handbook of biochem and mol.bio geralal .jasmen.8. Molecular biology by David Freifelder9. A genetic switch by Mark Pthasne.10. Microbial genetics by David Freifelder11. Essential of molecular biology by David Freifelder12. Principles of Genome analysis

S.B.Primose


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13. Bacteriophages by John Douglas14. Biotechnology an Introdoction by Susan R. Barnum.15. Biotechnology Volumes by H.J. Rehm & Reed16. Genes IX by lewin17. Molecular biology of the gene by J.D.Watson.18. Gene cloning and DNA analysis by T.A.Brown.19. From genes to Clone20.Molecular Biology Lab fax I & II : T. A. Brown21. Molecular Biotechnology Glick22.Molecular Genetics of Bacteria 4th Edition by Dale, J.W., Park, S.F. (2005) Wiley

and Sons Inc

23.Intellectual Property Rights on Biotechnology, by Sigh, KC BCIL, New Delhi24.Introduction to Modern Virology 4th Edition by Dimmock N J, Primrose S. B.

1994. Blackwell Scientific Publications. Oxford.

25.DNA Science by David .A.Micholas.


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CS703 - Communication Skills1

Credit Hours:

TEACHING SCHEME THEORY PRACTICAL TOTALHours/week 1 1 2Marks 50 100 150

A. Objectives:

1) To enhance basic Communication Skills i.e. Listening, Speaking, Reading, Writingof the students,

2) On an advanced stage, to improve their Speaking and Listening Skills necessary foreveryday living and for classroom, academic and cultural situations, and eventually3) To sharpen their expressional skills and help them succeed in Interview Process.

B. Outline of the Course:

Sr. No. Title of the Unit MinimumNumber ofHours

1 Elements of Communication and Principles of effectivecommunication

06

2 Key Communication Skills and Barriers to effective

communication

06

3 Common Communication Styles and How to Work withthem

08

4 Comprehensional Skills 155 Expressional Skills 156 Grammar and Vocabulary 10

Total Hours (Theory): 30Total Hours (Lab): 30Total: 60


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C. Detailed Syllabus:

Unit 1: Elements of Communication and Principles of effective communicationHours: 06 Weightage: 10%

Sr. No. Topic MinimumNumber ofHours

1 Definition, Concept and Process of Communication 12 Types and Levels of Communication 1 3 Principles of Effective Communication 24 Verbal Vs Nonverbal Communication 1

Unit 2: Key Communication Skills and Barriers to effective communication:

Hours: 06 Weightage: 10%


1 LSRW in Communication 12 Comprehensional and Expressional Skills 23 Barriers to effective Communication and techniques to

overcome them2

4 Alternative Modes of Communication 1

Unit 3: Common Communication Styles and How to Work with them:Hours: 08 Weightage: 13%


1 Introduction to Communication Styles 12 Assertive Communication 13 Aggressive Communication 14 Passive Communication 1

5 Passive Aggressive Communication 16 Working with different Styles 3


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Unit 4: Comprehensional Skills:Hours: 15 Weightage: 25%

Sr. No. Topic Min.No.ofHours

1 Basic Comprehensional Skills: Reading and Listening 12 Types and Techniques - Skimming and Scanning of

Reading5

3 Types of Listening 24 Tips for effective listening 25 Academic Listening Lectures and Presentations 5

Unit 5: Expressional Skills:Hours: 15 Weightage: 25%


1 Basic Expressional Skills: Writing and Speaking 2 Note Taking, Note Making, and Paragraph Development

Coherence, Topic Sentence, Supporting Sentence,Authentication, Examples

1

3 Letter Writing and Resume Making 24 Technical Report Writing 35 Email Drafting and Etiquettes 16 Delivering Effective Speeches 2

7 Participating in Group Discussions 2 8 Winning at Personal Interviews 2

Unit 6: Grammar and Vocabulary:Hours: 10 Weightage: 17%


1 Tenses and the concept of time 32 Active and Passive Constructions 13 Direct Indirect Speech 24 Prepositions 15 Conditionals 16 Idioms, Confusables, One-word Substitutes, Synonyms,

Antonyms2


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Recommended Study Material:

Text Books:1.Contemporary Business Communication by Scott Over2.Basic Business Communication, 10th Edition by Lesikar and Flatley3.Developing Communication Skills by Krishna Mohan & Meera Banerji

Reference Books:1. Written Communication in English by Freeman Sardh2. ABC of Common Grammatical Errors by Nigel B.Turton3. English for Technical Communication Volume 2&2 Combined by Laxminarayan R

K


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AP701 Seminar / Assignment / Miniprojects / Library


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SYLLABI(Semester 2)



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BT705 - IMMUNOLOGY

Total credits: 27 Semester - 2

A.Objective of the course.

To introduce the architecture of the immune system and various aspects ofimmunity and immune responses.

To understand the role of various cells and molecules in innate and adaptiveimmunity

To introduce to the concepts of antigens and antibodies, their types and structuresand how diversity is generated among antibody molecules.

To elaborate the role of B and T cells in humoral and cell mediated immunityTo understand the mechanisms of pathogeneses and elicitation of immune response

during various bacterial, parasitic and viral infections.To know in detail the implications of inflammation, hypersensitivity and immune

deficiencies on health of the host.

Understanding various reactions of antigens and antibodies and their applicationsTo apply the knowledge of behaviour of immune system and molecules involved

therein towards development of diagnostic methods and vaccines.


Sr. No Title of the units Minimum

number ofhours

1 Immune system and immunity 9h2 Mechanisms of host defense-I 9h3 Mechanisms of host defense-II 9h4 The immune system in health and disease 9h5 Immunotechnology 9h


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C. Detailed syllabus

Sr. No Title of the units Minimumnumberof hours

Unit 1 Immune system and immunity 9h 20%Innate and adaptive immunity, organs of the immunesystem, cells and molecules involved in innate andadaptive immunity, antigens and antibodies, structureand functions of antibody molecules, generation ofantibody diversity

Unit 2 Mechanisms of host defense-I 9h 20%Humoral and cell mediated immune responses, B andT cell epitopes, primary and secondary immunemodulation, antigen processing and presentation,MHC molecules

Unit 3 Mechanisms of host defense-II 9h 20%Activation and differentiation of B and T cells thecomplement system, immune effectors mechanisms,mechanisms of pathogeneses, immune responsesduring bacterial, parasitic and viral infections

Unit 4 The immune system in health and disease 9h 20%Inflammation, hypersensitivity and autoimmunity,immune deficiencies, transplantation immunology

Unit 5 Immunotechnology 9h 20%Antigen and antibody reactions, principles ofimmunodiagnostics, hybridoma technology,monoclonal antibodies, antibody engineering, vaccines









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performance.

E Student learning outcome

After the successful completion of the course a student will be able to:

Understand the immune system, various aspects of immunity and immune responsesand the role of various cells and molecules in the innate and adaptive immunity.

Imbibe the concepts of antigens and antibodies, their types and structures and howdiversity is generated in antibody molecules.

Elaborate the role of B and T cells in humoral and cell mediated immunity.Delineate the mechanisms of pathogeneses and immune response during variousbacterial, parasitic and viral infectionsKnow the implications of inflammation, hypersensitivity and immune deficiencies

on health of the host.

Understand the various reactions of antigens and antibodies.To apply the principles of immunological processes in the development of diagnostic

methods and vaccines.

F Recommended Study Material:

1. Kuby Immunology by Thomos J. Kindt, Richard A. Goldsby, Barbara A. Osborne,Sixth Edition W. H. Freeman and Company, New York (2007)

2. Immunobiology by Charles A. Janeway, Jr., Paul Travers, Mark Walport, Mark J.Shlomchik; Sixth Edition, Garland Science (2005)

3. Prescotts, Microbiology by Joanne M. Willey, Linda M. Sherwood, ChristopherJ. Woolverton, Seventh edition. McGraw Hill (2008).


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BT706 - GENETICS AND BIOCHEMISTRY


A. Objectives of the courseTo introduce subject of genetics by giving a brief historical overview and by

discussing the principles of Mendelian inheritance and its extensions.

To learn the use of mutations and complementation test in genetic analysis. To know various genetic disorders and their genetic basis. To understand the various mechanisms of gene transfer in microorganism and extra

chromosomal inheritance in detail.

To discuss in detail the structures and functions of various biomolecules withparticular emphasis on proteins.

To understand the pathways involved in metabolism of various biomolecules. To elaborate structure, functions and characteristics of enzymes and enzyme

kinetics.


Sr.No Title of the units Minimumnumber ofhours

1 Basics of genetics 9h2 Genetics of microorganisms 9h3 Structure and functions of biomolecules 9h4 Metabolism of biomolecules 9h5 Principles of enzyme catalysis 9h


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Unit 1 Basics of genetics 9h 20%Brief history, Mendelian principles and itsextensions, mutations and their use in geneticanalysis, complementation test, linkage, inheritancein mitochondria and chloroplast genes, pedigreeanalysis, genetic disorders

Unit 2 Genetics of microorganisms 9h 20%Methods of gene transfer in microorganisms,extrachromosomal genetic elements and their

mainteance, tetrad analysis

Unit 3 Structure and functions of biomolecules 9h 20%Composition, structure and functions ofcarbohydrates, lipids, proteins, conformations ofproteins, protein folding

Unit 4 Metabolism of biomolecules 9h 20%Concepts of metabolism, major metabolic pathwaysfor carbohydrates, lipids, amino acids, nucleotidesand vitamins, regulation of metabolism

Unit 5 Principles of enzyme catalysis 9h 20%

Structure and characteristics of enzymes, enzymecatalysed reactions, kinetics of enzyme catalyzedreactions, enzyme activation and inhibition,purification of enzymes

D. Instructional Methods and Pedagogy








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performance.



Understand the basic principles of Mendelian inheritance and its extensions. Know the use of mutations and complementation test in genetic analysis. Understand genetic disorders and their genetic basis. Develop insights into various mechanisms of gene transfer in microorganism and

extra chromosomal inheritance.

Get knowledge about the structures and functions of various biomolecules withparticular emphasis on proteins.

Understand the pathways involved in metabolism of various biomolecules andappreciate the complexities involved therein.

Understand in detail the structure, functions and characteristics of enzymes andenzyme kinetics.

F Recommended Study Material

1. Lehninger Principles of Biochemistry by David Nelson and Michael Cox , FifthEdition Freeman Company. (2005)

2. Biochemistry by Jeremy Berg, John Tymoczko and Lubert Stryer, Fifth Edition,Freeman Company;

3. Enzymes: Biochemistry, Biotechnology, Clinical Chemistry by Trevor Palmer. East-West Press Edition (2004)

4. Principles of Genetics by R. snusted, Wiley publication5. Principles of Genetic analysis by F.Griffith, W.H.Freeman6. Principles of Genetics by Tamarin, Mcgraw-Hill7. Principles of Genetics by R. snusted, Wiley publication8. Principles of Genetics by Tamarin, Mcgraw-Hill9. Molecular Genetics by Uldis Streips, Wiley Liss10.

Molecular Genetics of Bacteria by Robert Glass, Academic Press


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BT707 - BIOPROCESS TECHNOLOGY


A. Objective of the course.To introduce and appreciate the scope and future of bioprocess technologyTo learn how microorganisms can be screened for production of metabolites and

activities.

To understand how process fluids, air and other utilities can be sterilized andasceptic conditions maintained.

To understand on what basis cultivation methods are decided for a particularbioprocess.

To know the types of bioreactor configurations available and the principles of theirdesign

To understand the role of mass and heat transfer, aeration and mixing inbioprocesses.

To introduce to various methods of cultivation of microbial, plant and animal cells.To know how bioprocesses can be controlled.To elaborate the various methods available for recovery and purification of

biotechnological products



1 Basic concepts of bioprocess technology 9h2 Principles of reactor design and operation 9h3 Fundamentals of process design 9h4 Cultivation methods and process control 9h5 Recovery and purification of products 9h


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Sr.No Title of the units Min ofhours

Unit 1 Basic concepts of bioprocess technology 9h 20%Scope and future of bioprocess technology, processorganisms, screening for microorganisms/ activities,cultivation media and its optimization, sterilizationof process fluids, air and utilities, control ofcontamination, improvement of productivity, use ofrecombinant organisms in bioprocessing

Unit 2 Principles of reactor design and operation 9h 20%

Choosing the cultivation method, mechanically andnonmechanically agitated reactors, mass and heattransfer, aeration, mixing

Unit 3 Fundamentals of process design 9h 20%Stoichiometry and energetics of microbial growthand product formation, bioprocess kinetics, rheologyof process fluids, process scale-up and its difficulties

Unit 4 Cultivation methods and process control 9h 20%Batch, fed batch and continuous cultivation,immobilized cell systems, solid substrate

cultivation, mixed cultures, control of bioprocessesUnit 5 Recovery and purification of products 9h 20%Strategies to recover and purify products, separationof insoluble products, cell disruption, separation ofsoluble products, concentration of biologicalproducts with special emphasis on proteins, productformulation- microbial cells and soluble products

D Instructional Methods and Pedagogy:






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performance.



Know and appreciate the scope and future of bioprocess technology.Understand how microorganisms can be screened for production of metabolites andactivities.Understand how process fluids, air and other utilities can be sterilized and asceptic

conditions maintained.

Delineate on what basis cultivation methods are decided for a particular bioprocess.Know the types of bioreactor configurations available and the principles of their

design

Appreciate the role of mass and heat transfer, aeration and mixing in bioprocesses.Know various methods of cultivation of microbial, plant and animal cells and how

bioprocesses can be controlled.

To elaborate the various methods available for recovery and purification ofbiotechnological products


1. Biochemical engineering by Aiba, Humphrey and Millis . University of TokyoPress, Tokyo

2. Biochemical Engineering fundamentals by Bailey and Ollis. McGraw Hill Book Co.New York

3. Principles of Fermentation technology by Stanbury, Whitaker and Hall, PegamonPress, Oxford


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BT708 - INDUSTRIAL BIOTECHNOLGY


A. Objective of the course.To elaborate on the scope of biotechnology based products as compared to

chemically derived products.

To introduce to the role of regulatory, IPR and biosafety issues concerning thedevelopment of biotechnological industry

To give an overview of bioentrepreneurship and biotechnology industry in India.To understand the production of various primary and secondary metabolites,

bioinoculants, recombinant proteins, probiotics and neutraceuticals.

To provide insights into regulations in biotechnology.


Sr. No Title of the units Minimumnumber ofhours

BT/MI 708: INDUSTRIAL BIOTECHNOLGY

1 Introduction to business of biotechnology 9h2 Biotechnological products-I 9h3 Biotechnological products-II 9h4 Biopharmaceuticals and neutraceuticals 9h5 Introduction to regulations in biotechnology 9h


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C Detailed syllabus

Sr. No. Title of the units Minimumnumber ofhours

Unit 1 Introduction to business of biotechnology 9h 20%Scope of biotechnology based products, competitionwith chemical synthesis based products, economicregulatory and IPR issues, patenting of processesand products, biotechnology companies- care andnurturing, bioentrepreneurship, an overview of thebiotechnology industry in India

Unit 2 Biotechnological products-I 9h 20%Production and applications of amino acids (lysine

and glutamic acid), citric acid, ethanol, vitamin B12,xanthan gum

Unit 3 Biotechnological products-II 9h 20%Production and applications of amylase, rifampicin,lovastatin, bioinoculants

Unit 4 Biopharmaceuticals and neutraceuticals 9h 20%Production of recombinant proteins,biotransformations, probiotics and neutraceuticals

Unit 5 Introduction to regulations in biotechnology 9h 20%Manufacturing facility for biotechnology based

products-block flow diagram and conceptual layout,Good manufacturing practices- cGMP guidelines,principles of quality assurance and quality control,validation of facility, equipment and materials, biowaste treatment and disposal,

D Instructional Methods and Pedagogy:








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performance.



Elaborate on the scope of biotechnology based products as compared to chemicallyderived products.

Understand the role of regulatory, IPR and biosafety issues concerning thedevelopment of biotechnological industry.

Get an overview of bioentrepreneurship and biotechnology industry in India.Understand the production of various primary and secondary metabolites,bioinoculants, recombinant proteins, probiotics and neutraceuticals.Obtain insights into regulations in biotechnology


1. Comprehensive Biotechnology by Murray Moo-Young, H.W. Blanch, S. Drew,D.I.C (Eds). Wang Pergamon Press Ltd.

2. Biotechnology by H J Rehm and G Reed (Eds) Wiley-VCH (1985)3. Industrial Microbiology by Prescott and Dunn4. Industrial Microbiology by A H Patel5. Handbook of Enology Vol 1, 2nd Edition, Pascal Ribereau-Gayon, Denis

Dubourdieu, Bernard Don`eche, Aline Lonvaud (Eds), John Wiley & Sons Ltd.

(2000)

6. Industrial Microbiology, L E Casida (2007), New Age International Ltd.7. Basic Biotechnology, 3rd edition, Colin Ratledge (Ed) Cambridge University Press

(2006)

8. Handbook of Microbiological quality control- Pharmaceutical and Medicaldevices. Baird, Hodges and Denyer (Eds), Taylor and Francis, London (2000)

9. Quality in the manufacture of medicine and other health care products by JohnSharp (2000)


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BT710 - COMPUTATIONAL SKILLS


A. Objective of the course. To understand the basic concepts of mathematics relevant to biotechnology. To introduce to the concepts of modern computing with respect to the

computer architecture, input output devices, storage devices and variousoperating systems

To introduce to the database systems, creation of databases and various typesof databases

To provide an basic insights into computer programming with particularreference to biocomputing languages

To discuss various applications of computers in biological sciences such as useof computers and softwares in data plotting and analysis, use of web basedprograms for solving biological problems



hours

1 BASIC MATHEMATICS 3h2 BASIC CONCEPTS OF COMPUTING 3h3 INTRODUCTION TO DATABASE

SYSTEMS3h

4 COMPUTER PROGRAMMINGFUNDAMENTALS

3h

5 APPLICATIONS OF COMPUTERS INBIOTECHNOLOGY

3h


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Sr. No Title of the units Minimumnumberof hours

Unit 1 BASIC MATHEMATICS 9h 20%Functions and graphs, 2D coordinate geometry, 3Dgeometry, basic trigonometric functions, matrixalgebra

Unit 2 BASIC CONCEPTS OF COMPUTING 9h 20%Overview and concepts of a computer system, inputand output devices, storage devices, main circuitboard of a PC, memory, types of processing, types ofmodern computing, an overview of computer viruses,

introduction to operating systemsUnit 3 INTRODUCTION TO DATABASE SYSTEMS 9h 20%

Concepts of various types of databases, dataabstraction, data models, instances and schemes, E-Rmodel, Network data model, Hierachial data model,database software, text databases, multimediadatabases

Unit 4 COMPUTER PROGRAMMINGFUNDAMENTALS

9h 20%

Algorithms, computer languages, fundamentals ofprogramming, introduction to biocomputinglanguages- C and Perl

Unit 5 APPLICATIONS OF COMPUTERS INBIOTECHNOLOGY

9h 20%

Use of computers in solving biological problems,softwares for biological sciences: data plotting andanalysis, bioinformatics and biostatistics, the internetand its resources








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performance.

E. Student learning outcome


To understand the basic concepts of mathematics relevant to biotechnology.Understand the concepts of modern computing with respect to the computer

architecture, input output devices, storage devices and various operating systems

Know the concepts of database systems, creation of databases and various types ofdatabasesWill get basic insights into computer programming with particular reference to

biocomputing languages

Able to know the applications of computers in biological sciences such as use ofcomputers and softwares in data plotting and analysis, use of web based programsfor solving biological problems


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SYLLABI(Semester 3)



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BT 801 - ADVANCED GENETICS AND GENETIC ENGINEERING


A. Objective of the course To learn the basic principles and techniques of genome mapping and analysis. To understand the principles of population genetics and its applications in

conservation and medicine

To provide insights into basic concepts and applications of genetic engineering. To understand various aspects of genomics and proteomics



hours

1 Mapping and analysis of genomes 9h2 Population genetics and its applications 9h3 Concepts of genetic engineering I 9h4 Concepts of genetic engineering II 9h5 Genomics and proteomics 9h


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hours

Unit 1 Mapping and analysis of genomes 9h 20%Chromosome mapping in eukaryotes, phenotypic v/smolecular markers, different kinds of DNA markers forgenome analysis, mapping with molecular markers,mapping by using somatic cell hybrids, development ofmapping population in plants, mapping genes byinterrupted mating, studying gene location and structure,genome sequencing, human genome project, salientfeatures of human genome

Unit 2 Quantitative genetics and population genetics 9h 20%Inheritance of complex traits, analysis of quantitativetraits, heritability and its measurements, QTL mapping,the theory of allele frequencies, natural selection, randomgenetic drift, populations in genetic equilibrium, geneticconstitution of a population, basic principles ofconservation genetics, role of genomics in conservation,applications of population genetics

Unit 3 Concepts of genetic engineering I 9h 20%Enzymes for genetic engineering, vectors and hosts forcloning and expression, cloning strategies, cloning in

expression vectors, gene silencingUnit 4 Concepts of genetic engineering II 9h 20%

Construction of gene libraries, screening of libraries,promoter isolation and deletion studies, site directedmutagenesis, in vitro mutagenesis and deletion techniques,gene knock out in bacterial and eukaryotic systems,applications of genetic engineering, gene therapy

Unit 5 Genomics and proteomics 9h 20%The genome, transcriptome and proteome, introductionto genomics, comparative genomics, functional genomics,methods for gene expression analysis, metagenomics,introduction to proteomics, the need for proteomics;separation, identification, quantitation and analysis ofproteins for proteomic studies, interaction proteomics,applications of genomics and proteomics


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performance.

E. Student learning outcomes


1. Understand the basic principles and techniques of genome mapping and analysis.2. Know the principles of population genetics, its applications in conservation and

medicine3. Get insights into basic concepts of genetic engineering and its applications4. Understand various aspects of genomics and proteomics and their applications.

F. Recommended Study Material

1. Principles of genetics by D.P. Snustad and M. J. Simmons, Fifth edition, JohnWiley and Sons, Inc. 2010

2. Gene cloning and manipulation by C. Howe, Cambride, 2007.3. Genetic Engineering by S. Mitra, MacMillan, 20054. Genomics fundamentals and applications by S. Choudhuri and D. B. Carlson,Informa, 20095. Microbial functional genomics. Zhou et al., John Wiley and Sons, 20046. Introduction to genomics by A. Lesk, Oxford University Press, 2007.7. Principles of Gene Manipulation and Genomics, Third Edition (2006) S.B.

Primrose, S.B. and R.M. Twyman, Blackwell Publishing Company, Oxford, UK. 2.8. Gene Cloning and DNA Analysis: An Introduction. Fifth Edition (2006) T.A.

Brown, Wiley-Blackwell, UK.9. Molecular Cloning. A Laboratory Manual. Volume 1-3. Third Edition (2001) A.

Sambrook and D.W. Russell, Cold Spring Harbor Laboratory Press, New York,USA.

10. Principles of proteomics by R. M. Twyman, Taylor and Francis, 2008.


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BT802 - PLANT AND ANIMAL BIOTECHNOLOGY


A. Objectives of the course

To understand the basic concepts of plant and animal development.To understand the applications of genetically engineered plant and animals.To train students on theoretical and practical aspects of plant tissue culture by

providing case studies.


number ofhours

1 Basic concepts of development 9h2 Plant development 9h3 Animal development 9h4 Genetic engineering of plant and animals 9h5 Plant tissue culture and its applications 9h


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C. Detailed syllabusSr.No Title of the units Minimum

number ofhours

Unit 1 Basic concepts of development 9h 20%Potency, commitment, specification, induction,competence, determination and differentiation,morphogenetic gradients, cell fate and cell lineages,stem cells, genomic equivalence and the cytoplasmicdeterminants, imprinting, mutants and transgenicsin analysis of development

Unit 2 Principles of plant development 9h 20%Embryo sac development and double fertilization in

plants, embryogenesis, establishment of symmetry inplants, seed formation and germination, shoot androot development, leaf development, transition toflowering, floral development in Arabidopsis,senescence

Unit 3 Principles of animal development 9h 20%Production of gametes, cell surface molecules insperm egg recognition in animals, zygote formation,cleavage, blastula formation, embryonic fields,gastrulation and formation of germ layers in animals,axes and pattern formation in Drosophila, amphibian

and chick, organogenesis, differentiation of neurons,environmental regulation of normal development

Unit 4 Transgenic plants and animals 9h 20%Introduction to transgene technology, transgenicplants and animals; gene transfer strategies for plantand animals, molecular farming and breeding,marker assisted breeding in plants and animals,improvement of plant and animals for specific traitsusing transgene technology- case studies,regulations for use of transgenic plant and animals

Unit 5 Plant tissue culture and its applications 9h 20%Plant tissue culture, culture media and growthhormones, micropropagation, somaticembryogenesis, anther culture, somatichybridization, somaclonal variation, applications ofplant tissue culture with case studies.


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D.Instructional Methods and Pedagogy:The topics will be discussed in interactive class room sessions using classical black-board







performance.



Understand the basic concepts of plant and animal development.Understand the use of genetic engineering of plant and animals and their

limitations.Get insights into the regulations for the use of genetically modified organisms.Get trained on theoretical and practical aspects of plant tissue culture by providing

case studies.

F. Recommended Study Material:

1. Introduction to Plant Tissue Culture by MK Razdan2. Experiments in Plant Tissue Culture by John H Dodds, Lorin W Roberts3. Plant Tissue Culture by S. S. Bhojwani & M. K. Razdan

4. Plant Anatomy by Esau5. Plant Physiology by Lincoln Taiz, Eduardo Zeiger6. Patterns in plant developmentby Taylor A. Steeves, Ian M. Sussex7. Mechanisms in plant developmentby Ottoline Leyser and Stephen Day8. Molecular genetics of plant developmentby Stephen H. Howell and Stephen HerbertHowel9. Plant biotechnology-The genetic manipulation of plants by Adrian Slater et al., OxfordUniversity Press, 2003.


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BT803 - ENVIRONMENTAL BIOTECHNOLOGY AND BIOINFORMATICS


A. Objective of the courseTo provide knowledge regarding various forms of pollution, its monitoring and

methods for its control.

To understand the biodegradation of various recalcitrant compounds bymicroorganisms.

To learn various strategies for bioremediation of polluted sites and wastes.To provide basic insights into various bioinformatics tools and techniques.To understand the use of bioinformatics tools and techniques in research and

industry.

B. Outline of the CourseSr.No Title of the units Minimum

number ofhours

1 Pollution, its monitoring and control 9h2 Biodegradation of pollutants 9h3 Bioremediation 9h4

Essentials of bioinformatics I9h

5 Essentials of bioinformatics II 9h


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C. Detailed syllabusSr.No Title of the units Minimum

numberof hours

Unit 1 Pollution, its monitoring and control 9h 20%Scope of environmental biotechnology, pollution andits types, effects of pollution on health andenvironment, environmental monitoring, overview ofenvironmental laws in India, pollution controlstrategies

Unit 2 Biodegradation of pollutants and recalcitrant

compounds

9h 20%

Recalcitrant molecules, basis for recalcitrance,xenobiotic compounds, growth linkedbiodegradation and cometabolism, effect of chemicalstructure on biodegradation, biodegradation ofhydrocarbons, dyes, pesticides, lignin, biodegradableplastics

Unit 3 Bioremediation 9h 20%Bioavailability, approaches to bioremediation, wastecharacterization methods, methods of liquid andsolid waste treatment and disposal

Unit 4 Essentials of Bioinformatics I 9h 20%Introduction to bioinformatics-goals, scope,applications and limitations, introduction tobiological databases, bioinformatics tools available,extraction of knowledge from resources, scoringmatrices

Unit 5 Essentials of Bioinformatics II 9h 20%sequence based bioinformatics tools, phylogenetictree construction methods and programs Structureanalysis and validation, 3 D structure visualizationof protein, classification and comparison of protein

3D structures


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D. Instructional Methods and PedagogyThe topics will be discussed in interactive class room sessions using classical black-board







performance.



Knowledge regarding various categories of pollution, its monitoring and methodsfor control will be obtained.

Understanding of mechanisms of biodegradation of various recalcitrantcompounds will be gained. Various strategies involved in bioremediation will be known. Basic insights into bioinformatics tools and techniques and their application in

research and industry will be gained

F. Recommended Study Material

1. Environmental Biotechnology by Alan Scragg, Oxford University Press, 2005.2. Environmental Biotechnology by B. Bhattacharya, Oxford University Press, 2007.

3. Biodegradation and Bioremediation by Martin Alexander, Academic Press, 1999.4. Essential Bioinformatics by Jin Xiong, Cambridge University Press, 2006.5. Environmental Biotechnology by G. M. Evans and J. C. Furlong, Wiley, 2003.6. Bioinformatics-An introduction, J.J.Ramsden, Springer, 2009.7. Introduction to bioinformatics by A. Lesk, Oxford University Press, 2008.


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BT804 - ADVANCED BIOPROCESS TECHNOLOGY ANDBIOPHARMACEUTICALS


A. Objective of the courseTo understand concepts of metabolic engineering and systems biology.To provide insights regarding the various process related considerations in the

cultivation and exploitation of plant and animal cells.

To provide basic knowledge of drug discovery and development approaches.To provide in depth knowledge of various biopharmaceuticals and their purification

and formulation techniques.


numberof hours

1 Metabolic engineering and systems biology 9h2 Bioprocess considerations in using animal and plant

cell cultures9h

3 Drug design and development 9h4 Biopharmaceuticals 9h5 Downstream processing of biopharmaceuticals 9h


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C. Detailed syllabusSr.No Title of the units Minimu

mnumberof hours

Unit 1 Metabolic engineering and systems biology 9h 20%Improvement of microbial strains and bioprocesses,overproduction of microbial products, geneticallyengineered organisms in bioprocesses, scaling up ofgenetically engineered cell culture fermentation, conceptsof systems biology, introduction to flux control analysis,metabolic control analysis, fermentation modeling

Unit 2 Bioprocess considerations in using animal and plant cellcultures

9h 20%

Approaches for cultivation of algal, plant and animal cells,comparison with cultivation of microbial cells, bioreactorconsiderations, stem cell cultivation, methods formicroalgal cultivation, products of animal cell cultures,medical applications of bioprocess engineering, biofuels,fermentation economics

Unit 3 Drug discovery and development 9h 20%Introduction to drug discovery and development, sourcesof drugs, approaches to new drug discovery, role ofmolecular recognition in drug design, enzymes andreceptors as drug targets, prodrug design and applications,

computer aided drug design, preclinical and clinical trialsUnit 4 Biopharmaceuticals 9h 20%

Concepts of pharmaceuticals, biologics andbiopharmaceuticals, sources of biopharmaceuticals,biopharmaceuticals in production and research- cytokines,hemopoetic growth factors, hormones, blood products,therapeutic enzymes, nucleic acid therapeatutics, casestudies

Unit 5 Downstream processing of biopharmaceuticals 9h 20%Principles of protein separation, intracellular andextracellular proteins, protein stability and solubility,various protein purification techniques at industrial scale,case studies for purification of biopharmaceuticals,formulation of biopharmaceutical products, proteinengineering


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D. Instructional Methods and Pedagogy:The topics will be discussed in interactive class room sessions using classical black-board







performance.



To know the concepts of metabolic engineering and systems biology.Insights regarding the various process related considerations in the cultivation and

exploitation of plant and animal cells will be gained.To understand the drug discovery strategies and development approaches.To provide in depth knowledge of various biopharmaceuticals and their purification

and development techniques.


1. An introduction to drug design by S. N. Pandya, New Age International publishers2. Fermentation microbiology and biotechnology by E.M.T. El Mansi and C.F.A. Bryce.Taylor and Francis, 1999.

3. Text book of Drug design and discovery by Larsen, Taylor and Francis, 2002.4. Biopharmaceuticals by Gary Walsh5. Biopharmaceutical drug design and development by Pong and Rojanasakul, HumanaPress, 2008.


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msc bt booklet

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