Mr. AltorferLife Science

The Parts of the Compound Light Microscope

The Compound Light Microscope

• Simple Microscopes- Microscopes with only one lens.

• Example: Magnifying Glass, Glasses

http://player.discoveryeducation.com/index.cfm?guidAssetId=4b7b0c5c-07cd-4b5a-b196-c8ffd4dcb099

The Compound Light Microscope

• Compound Light Microscope- A microscope that uses two or more lenses to magnify an object.

• Examples:

http://player.discoveryeducation.com/index.cfm?guidAssetId=4b7b0c5c-07cd-4b5a-b196-c8ffd4dcb099http://player.discoveryeducation.com/index.cfm?guidAssetId=4b7b0c5c-07cd-4b5a-b196-c8ffd4dcb099

Body Tube

The Compound Light Microscope

• Body Tube- The part of the microscope that you look through with your eye.

Body Tube

Revolving Nose piece

The Compound Light Microscope

• Revolving Nose Piece- Allows the objective lenses to be rotated into position.

Body Tube

Revolving Nose piece

Scanning Lens

The Compound Light Microscope

• Scanning Lens- The smallest of the three objective lenses. Allows the user to see a very big picture.

• Very little detail is seen with this lens.

• Always use this lens first!

• Magnification level- 4X

Body Tube

Revolving Nose piece

Scanning Lens

Low Power Lens

The Compound Light Microscope

• Low Power Lens- Use this lens to see greater detail in a specimen.

• Use this lens second!

• Magnification level: 10X

Body Tube

Revolving Nose piece

Scanning Lens

Low Power Lens

High Power lens

The Compound Light Microscope

• High Power Lens- The lens with the highest magnification level. This lens provides the highest level of detail.

• Use this lens last!

• Magnification Level- 40X

Body Tube

Revolving Nose piece

Scanning Lens

Low Power Lens

High Power Lens

Stage Clips

The Compound Light Microscope

• Stage Clips- Use these to hold the slide in place.

Body Tube

Revolving Nose piece

Scanning Lens

Low Power Lens

High Power Lens

Stage Clips

Diaphragm

The Compound Light Microscope

• Diaphragm- Use this to control the amount of light that passes through the specimen.

Body Tube

Revolving Nose piece

Scanning Lens

Low Power Lens

High Power Lens

Stage Clips

Diaphragm

Light Source

The Compound Light Microscope

• Light Source- Illuminates the object being viewed.

Body Tube

Revolving Nose piece

Scanning Lens

Low Power Lens

High Power Lens

Stage Clips

Diaphragm

Light Source

Eye Piece

The Compound Light Microscope

• Eye Piece- The part of the microscope that you view the specimen through.

• Magnification Power- 10X

Body Tube

Revolving Nose piece

Scanning Lens

Low Power Lens

High Power Lens

Stage Clips

Diaphragm

Light Source

Eye Piece

Arm

The Compound Light Microscope

• Arm- The part of the microscope that supports the revolving nose piece.

• Use the arm to carry the microscope.

Body Tube

Revolving Nose piece

Scanning Lens

Low Power Lens

High Power Lens

Stage Clips

Diaphragm

Light Source

Eye Piece

Arm

Stage

The Compound Light Microscope

• Stage- The glass slide is placed on the stage. Keep the stage clean and dry.

Body Tube

Revolving Nose piece

Scanning Lens

Low Power Lens

High Power Lens

Stage Clips

Diaphragm

Light Source

Eye Piece

Arm

Stage

Coarse Adjustment

The Compound Light Microscope

• Coarse Adjustment- Use this knob to focus the microscope under scanning and low power.

• NEVER use this knob under high power.

Body Tube

Revolving Nose piece

Scanning Lens

Low Power Lens

High Power Lens

Stage Clips

Diaphragm

Light Source

Eye Piece

Arm

Stage

Coarse Adjustment

Fine Adjustment

The Compound Light Microscope

• Fine Adjustment Knob- Use this knob to focus the microscope under any power.

• This knob sharpens the image you are viewing.

Body Tube

Revolving Nose piece

Scanning Lens

Low Power Lens

High Power Lens

Stage Clips

Diaphragm

Light Source

Eye Piece

Arm

Stage

Coarse Adjustment

Fine Adjustment

Base

The Compound Light Microscope

• Base- Supports the microscope. Always keep the base flat on the table.

• Never tilt the microscope.

Determining the Magnification of your Microscope-

• To determine the magnification of your microscope:

Power of the eye piece- 10xPower of the scanner 4 x objective lens-

Multiply them together- 40x is the total

magnification!

Determining the Magnification of your Microscope-

• Low Power Objective- 10x

Eye Piece- 10x

Total Magnification - 100x

Determining the Magnification of your Microscope-

• High Power Objective- 40x

Eye Piece- 10x

Total Magnification- 400x