IMPROVE DIGESTIBILITY | LOWER FEED COSTS | HIGHER QUALITY MEAT | HIGHER NET PROFITS

MORE HIGH QUALITY MEAT WITH LESS FEED BY USING ADVANCED MULTI-ENZYME TECHNOLOGY

BIOPROTON PTY LTDABN 19 059 093 417

Head Office:38/141 Station RoadSunnybank, Brisbane 4109 QLD, AUSTRALIA

Tel: + 61 7 3345 9115Tel: + 61 7 3344 1490Fax: + 61 7 3345 9582

Email: natuzyme@bioproton.comweb: www.bioproton.com

NATUZYME - HIGH QUALITY - GOOD RESULTS

NATUZYME HIGH QUALITY GOOD RESULTS

BIOPROTON PTY LTDBioproton is an Australian based biotechnology company with core business in developing; manufacturing and marketing high quality feed enzyme supplements. The company was founded in 1984 in Finland. In 1993 the company relocated to the fast growing Asia-Pacific region and today the head-office, product development and manufacturing operations are based in Brisbane, Australia. Bioproton has a global marketing and distribution network covering Africa, Asia, Europe, Russia, Middle East, North and South America. Customers can be supplied from facilities in Brisbane, Australia or Atlanta, Georgia, USA.

NATUZYME Natuzyme is a high quality & multi-activity feed enzyme formulated specifically for broiler, layer, duck, turkey, ruminant and aqua feeds to enable better nutrient utilisation, resulting in more high quality meat, eggs or milk with lower total costs.

Natuzyme contains cellulase, xylanase, beta-glucanase, mannanase, alpha-amylase, protease, lipase and phytase and other enzymes. The enzymes have a wide pH range, long-term stability and high temperature tolerance with high levels of enzyme activity enabling Natuzyme to be successfully applied to animal feeds with excellent results under wide ranging conditions around the world.

Many plant based feeds contain substrates with Anti-Nutritional Factors (ANFs) that reduce animal growth. Natuzyme enzymes improve the digestibility of these ANFs by degrading fibres, proteins, starches and phytates in feed to become digestible enabling the use of cheaper and often locally produced feeds while maximising meat, egg or milk productivity.

WHY USE MULTI-ENZYME NATUZYMEA single enzyme like phytase will only release phosphorous stored in phytate but not extract energy from feed. Feed qualities (NSP content, protein, energy, fat, etc) and feed prices can fluctuate significantly and unexpectedly. For these reasons, only a multi-enzyme approach will fully capture nutrition value by degrading all ANFs in all circumstances. In response to this, Bioproton developed Natuzyme in the 80s and 90s to provide Simplicity, Cost Effectiveness and Flexibility in feed enzyme use. We continue R&D with our partner, University of Queensland’s Australian Institute for Bioengineering and Nanotechnology (AIBN) in developing and producing feed enzymes with improved characteristics, which we incorporate into Natuzyme at strategic points.

BENEFITS OF NATUZYME ENZYMESNatuzyme has many enzyme activities included in the feed mix. The table below describes each of them and their functions in improving the nutritional content of feed.

Below is more information about two of the common ANFs in animals’ feeds.

NSP (Non-Starch Polysaccharides)Many plant based animal feeds contain long, complex carbohydrate or fibrous molecules known as non-starch polysaccharides (NSPs). It is well recognized that NSPs have an ANF impact. NSPs increase the digestion viscosity, which means that the animals own enzymes have a harder time locking and absorbing these nutrients, but they also encapsulate some nutrients making them unavailable. The inclusion of Natuzyme, which has NSP enzymes, allows the breakdown of these ANFs in feed and leads to improved digestion, resulting in greater feed efficiency.

Phytate and PhosphorousPhosphorous is a key nutritional requirement for animals to provide bone growth. Most of the phosphorus contained in animal feed of plant origin exists in the storage form phytate. Poultry and swine cannot digest phosphorus contained within phytate, since they lack phytase enzyme that breaks down this phytate molecule. Therefore the inclusion of the phytase enzyme in Natuzyme is required for release of phytate-bound phosphorus. This enables the reduction of Inorganic Phosphorous, which is most commonly applied in the form of DiCalcium Phosphate (DCP) into animal feed.

Enzyme Substrate Function Total Benefits

Phytase Phytate Release phosphorus stored in phytate Improve Feed Conversion

Protease Protein Improve protein digestibility Improve Litter (Less wet Droppings and Odour)

Pectinase NSP Breaks down indigestible Pectin (reduces digestive viscosity) Greater Uniformity

Xylanase NSP Breaks down fibre Reduce Feed Costs

Cellulase NSP Breaks down fibre Use Cheaper Feeds

Beta-Glucanase NSP Breaks down fibre Reduce DCP use and costs

Mannanase NSP Breaks down fibre Reduce Feed Costs

Alpha-Amylase Starch Improves starch digestibility Reduce Phosphorous and Nitrogen Excretion

Lipase Fat Improve fat absorption Improve Animal Wellbeing (reduces mortality and need for antibiotic use)

Broiler Trial - USA Diet: Corn / Soybean MealSpecies: BroilerLocation: Georgia (Southern Poultry Research), 2010Protocol: Evaluate the effectiveness of different enzyme products (1. Natuzyme, 2. Enzyme R + Phytase, 3. Enzyme A + Phytase) in Negative Control (NC) Diet vs. Positive Control (PC) Diet over a 42 day period. PC Diet: A typical corn / soybean meal used in Mid-West USA.NC Diet: PC less 140 Kcal/kg ME and less 0.1% Available Phosphorous

Results: Natuzyme provided superior outcome without needing phytase.

Broiler Trial – AustraliaDiet: Diet either wheat or sorghum.Species: BroilerLocation: University of Queensland, 2011Protocol: Measure AME (Apparent Metabolisable Energy) impact of Natuzyme either wheat / sorghum diet

Results: In both Sorghum diets: Natuzyme improved AME by 2.4% (and was statistically significant) In Wheat diets: Natuzyme improved AME by 1.9% (and was statistically significant)

Broiler Trial - USA Diet: Corn / Soybean Meal Species: Broiler Location: Georgia (Southern Poultry Research), 2010 Protocol: Evaluate the effectiveness of different enzyme products (1. Natuzyme, 2. Enzyme R +

Phytase, 3. Enzyme A + Phytase) in Negative Control (NC) Diet vs. Positive Control (PC) Diet over a 42 day period.

PC Diet: A typical corn / soybean meal used in Mid-West USA. NC Diet: PC less 140 Kcal/kg ME and less 0.1% Available Phosphorous Results: Natuzyme provided superior outcome without needing phytase. Broiler Trial – Australia Diet: Diet either wheat or sorghum. Species: Broiler Location: University of Queensland, 2011 Protocol: Measure AME (Apparent Metabolisable Energy) impact of Natuzyme either wheat / sorghum

diet Results: In both Sorghum diets: Natuzyme improved AME by 2.4% (and was statistically significant)

In Wheat diets: Natuzyme improved AME by 1.9% (and was statistically significant)

ab Means in column followed by different superscript are significantly different (p<0.05)

Diet Group AME, MJ/kg DM Improvement AME, MJ/kg DM Improvement, %

Control 14.38ab - Sorghum Natuzyme 14.73a 0.35 2.4% Control 13.92b - Wheat Natuzyme 14.18ab 0.26 1.9%

Broiler Trial – BrazilDiet: Diet corn / SMB (NC Diets = PC Less 140 Kcal/kg of ME).Diets: T1: Positive Control, T2: Negative Control, T3: T2+Natuzyme (made by Bioproton), T4: T2+ Competi tor Multienzyme Product.Species: BroilerLocation: Brazil, 2012Protocol: Compare FCR of two multienzyme products in commercial broiler diets.Temperature: Feed Pelletisation Temperature was 82C (Recorded by Laser)Results: Natuzyme outperformed Competitor Multienzyme Product by 4.52% and provided equal benefit for to match PC Diet.

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Compara've  Broiler  Trial  Data  

Brazil  2012  

Parameters  T1   T2   T3   T4   Average  

Daily  feed  intake   113.56a   114.00a   113.52a   116.29a   114.34  

Daily  body  weight  gain     63.03a   59.00b   62.83a   61.58ab   61.61  

Feed  Conversion   1.80a   1.93b   1.81a   1.89ab   1.86  Mortality  

0.01   0.02   0.02   0.01   0.02  

Broiler Trial – New ZealandDiet: Commercial wheat and sorghum diets.Species: BroilerLocation: New Zealand, 2012Protocol: Compare FCR of leading NSP enzyme products in commercial feedsResults: In Sorghum diets: Natuzyme FCR was 2.44% better than typical competitor In Wheat diets: Natuzyme FCR was 0.9% better than typical competito

Broiler Trial - Thailand Diet: Corn / Soybean MealSpecies: Broiler (Starter/Grower/Finisher)Location: Kasetsart University Thailand 2005Protocol: To evaluate Natuzyme in a Metabolisable Energy (ME) deficient starter/grower/finisher Broiler feed diets v. control diet.

Results: Natuzyme at 350grams/ton enables the following ME reduction without detrimental effects compared to the control diet of a broiler diet.

Layer Trial – RussiaDiet: basal diet with combination of wheat, barley, oat, soybean and sunflowerSpecies: Hisex WhiteLocation: Sinyavina Farm (Russia), 2011Protocol: To compare the effectiveness of Natuzyme and Commercial Phytase product in diets that had available phosphorous (aP) reduced by 0.1%

Results: Natuzyme was demonstrated to be more effective than just pure phytase use.

Layer Trial - Ukraine Diet: A basal diet with combination of wheat, barley, oat, soybean and sunflower.Species: LayerLocation: Ukraine, 2010Protocol: Natuzyme was added “on-the-top” and compared to diet without enzymes in layers over a 30d period.

Results: Natuzyme group increased egg production by 3.6 % and reduced the number of dirty eggs from 6.1% to 2.5%.

Compara've  Broiler  Trial  A  

1.369  

1.360  

1.369  

1.347  

1.327  

1.366  

1.373  

1.361  

1.300  

1.310  

1.320  

1.330  

1.340  

1.350  

1.360  

1.370  

1.380  

FCR  -­‐  Sorghum  Feeds  

1.436  

1.422  1.426  

1.404  

1.398   1.400  

1.409   1.407  

1.370  

1.380  

1.390  

1.400  

1.410  

1.420  

1.430  

1.440  

FCR  -­‐  Wheat  Feeds  

New  Zealand  2012  

Broiler Trial - Thailand Diet: Corn / Soybean Meal Species: Broiler (Starter/Grower/Finisher) Location: Kasetsart University Thailand 2005 Protocol: To evaluate Natuzyme in a Metabolisable Energy (ME) deficient starter/grower/finisher

Broiler feed diets v. control diet. Results: Natuzyme at 350grams/ton enables the following ME reduction without detrimental effects

compared to the control diet of a broiler diet. Starter: - 2.5% (or 77.5 Kcal ME/kg) Grower: - 5.0% (or 157.5 Kcal ME/kg) Finisher: - 7.5% (or 240.0 Kcal ME/kg)

Layer Trial - Ukraine Diet: A basal diet with combination of wheat, barley, oat, soybean and sunflower. Species: Layer Location: Ukraine, 2010 Protocol: Natuzyme was added “on-the-top” and compared to diet without enzymes in layers over a

30d period. Results: Natuzyme group increased egg production by 3.6 % and reduced the number of dirty eggs

from 6.1% to 2.5%.

Egg Production

Change in Egg Production

(%)

Dirty Eggs (%)

Control 23369 - 6.1% Natuzyme 24237 3.6% 2.5%

Stage Diet ME Reduction (Kcal ME/kg)

Weight Gain (Grams)

FCR

Control (3,100 Kcal ME/kg) - 710.7 1.5 Starter (0-21 DOA) -2.50% ME + Natuzyme 77.5 684.8 1.6

Control (3,150 Kcal ME/kg) - 1202.3 2.5 Grower (22-42 DOA) -5.00% ME + Natuzyme 157.5 1195.0 2.5

Control (3,200 Kcal ME/kg) - 302.1 4.3 Finisher (43-49 DOA) -7.50% ME + Natuzyme 240.0 303.5 4.4

Broiler Trial - Thailand Diet: Corn / Soybean Meal Species: Broiler (Starter/Grower/Finisher) Location: Kasetsart University Thailand 2005 Protocol: To evaluate Natuzyme in a Metabolisable Energy (ME) deficient starter/grower/finisher

Broiler feed diets v. control diet. Results: Natuzyme at 350grams/ton enables the following ME reduction without detrimental effects

compared to the control diet of a broiler diet. Starter: - 2.5% (or 77.5 Kcal ME/kg) Grower: - 5.0% (or 157.5 Kcal ME/kg) Finisher: - 7.5% (or 240.0 Kcal ME/kg)

Layer Trial - Ukraine Diet: A basal diet with combination of wheat, barley, oat, soybean and sunflower. Species: Layer Location: Ukraine, 2010 Protocol: Natuzyme was added “on-the-top” and compared to diet without enzymes in layers over a

30d period. Results: Natuzyme group increased egg production by 3.6 % and reduced the number of dirty eggs

from 6.1% to 2.5%.

Egg Production

Change in Egg Production

(%)

Dirty Eggs (%)

Control 23369 - 6.1% Natuzyme 24237 3.6% 2.5%

Stage Diet ME Reduction (Kcal ME/kg)

Weight Gain (Grams)

FCR

Control (3,100 Kcal ME/kg) - 710.7 1.5 Starter (0-21 DOA) -2.50% ME + Natuzyme 77.5 684.8 1.6

Control (3,150 Kcal ME/kg) - 1202.3 2.5 Grower (22-42 DOA) -5.00% ME + Natuzyme 157.5 1195.0 2.5

Control (3,200 Kcal ME/kg) - 302.1 4.3 Finisher (43-49 DOA) -7.50% ME + Natuzyme 240.0 303.5 4.4

Layer Trial – Russia Diet: basal diet with combination of wheat, barley, oat, soybean and sunflower Species: Hisex White (Layers) Location: Sinyavina Farm (Russia), 2011 Protocol: To compare the effectiveness of Natuzyme and Commercial Phytase product in diets that had available phosphorous (aP) reduced by 0.1% Results: Natuzyme was demonstrated to be more effective than just pure phytase use.

Measured Characteristic

Unit of Measure

Control + 500FTU Phytase

Control + 350g/ton Natuzyme

Improvement

Mortality % 2.25% 2.24% -0.44%

Average Weight of Egg

Gram 63.47 63.48 0.02%

Productivity % 92.46% 92.68% 0.24%

Average Feed Intake

Gram/bird/day

116.26

116.18

-0.07%

Dirty Eggs % 19.93% 19.48% -2.26%

Feed Cost (Per 100,000 birds)

$US/day

$4,616

$4,547

-1.48% (-US$68.2)

Value of Eggs (Per 100,000 birds)

$US/day

$6,472

$6,488

0.24% (+US$15.4)

Profit (Per 100,000 birds)

$US/day

$1,857

$1,940

4.50% (+US$83.6)

Profit Margin % 40.2%

42.7%

6.07%

QUALITY ASSURANCE IN MANUFACTURING

To ensure highest levels of quality assurance Bioproton follows current Good Manufacturing Practices (cGMP) and is regulated by APVMA (Australian Pesticides and Veterinary Medicines Authority). This ensures Natuzyme is manufactured consistently, by a specified method, under adequate supervision and with effective quality control procedures.

• All operations are designed to avoid contamination, formation of undesirable by-products, deterioration and handling errors. The principles of cGMP include systems of quality control assurance, employee qualifications, maintenance standards for equipment, control of raw materials and product stability.• Identity control and laboratory management of the production strain. • Control of pure culture and operational parameters during fermentation. • Hygienic control during enzyme recovery.• Natuzyme is packed in an automatic computer controlled packaging line into three fold paper and one fold PE (polyethylene) bags, which are sealed at both ends.• The pallet is shrink-wrapped with several layers against moisture. Shipping mark is attached.• The Natuzyme is assayed for pharmacological, microbiological, toxicological, physio-chemical quality including TVC (total viable counts), coliforms and salmonella and heavy metals including lead, and for enzyme activity (a Certificate of Analysis and Certificate of Formulation is issued) before being released by Quality Control.

BIOPROTON PARTNERS

Growth in global food demand is placing pressure on the global agricultural sector to deliver safe, efficient and environmentally sustainable food to meet the demands of current and future generations. Enzyme supplementation to animal feeds plays a critical role in meeting this challenge. Bioproton, a feed enzyme manufacturer, is collaborating with the University of Queensland’s Australian Institute for Bioengineering and Nanotechnology (AIBN) in developing and producing feed enzymes with improved characteristics.

AIBN is an integrated multi-disciplinary research institute bringing together the skills of world-class researchers in the areas of bioengineering and nanotechnology. It is home to 19 research groups working at the interface of the biological, chemical and physical science to alleviate current problems in human health and environmental issues.

MILKING COW RESULTS

Milking Cow Trial – EgyptDiet: Normal local grass fed diet in Egypt. Species: Milking CowsLocation: Egypt, 2009Protocol: Measure the increase in milk production with Natuzyme use in milking cows in separate trial periods (A+B). Natuzyme added at 20g/head/day.Results: By using Natuzyme multi-enzyme formulation, it was demonstrated that Milk Production increased 2.43% and 1.67% respectively and Peak Milk Production period was extended

Swine Trial – United States of America (USA) Diet: Corn / Soybean MealSpecies: Weanling pigsLocation: Kansas State University, 2007Protocol: To evaluate the effectiveness of Natuzyme in both Positive Control (PC) and Negative Control (NC) Diets in a 35d trial on weanling pig performance. PC Diet: A typical corn / soybean meal used in Mid-West USA, NC Diet: PC but with 12.5% Soy Hulls and no antibiotics (34kcal/kg less ME and 1.1% less Protein).Results: Natuzyme enabled the use of cheaper and lower quality feeds (less energy and protein and no antibiotics) on a dose rate of 500g/ton.

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THERMOSTABILITYThermo-stability is an important parameter as enzymes are heat-sensitive proteins. Proteins denature (unwind) at high temperatures because their tertiary structures fall apart. Natuzyme has been engineered to withstand denaturing at high temperatures, enabling it to be included into animal feed prior to steam pelleting.

Thermo-stability TrialTrial Locations: USA 2010, South Africa 2011, and Brazil 2011Xylanase and Phytase (two key enzymes in Natuzyme) show >95% recovery in commercial feed milling trials.

ENZYME ACTIVITIESCellulaseXylanaseb-Glucanasea-AmylaseMannanaseProteasePectinasePhytaseLipaseAnd other enzyme activities

Note that Enzyme formulation may vary to ensure optimal enzyme formulation for local feed conditions.

APPLICATIONUpgrading feed efficiency of cereal diets on poultry (layers and broilers), turkeys, aqua and ruminant feeds.Natuzyme can be applied into many different feeds corn / soy / wheat / barley / rice (bran) / wheat (bran) / DDGS and other locally available ingredients.

RECOMMENDED DOSE RATETypical dosage rate is 350g/Ton of feed for broilers and 250g/Ton of feed for layers. The dose rate for swine is 350 - 500g/Ton of feed.

PRODUCT INFORMATIONMicro-granulated enzymesResistance to heat pelleting of up-to 90°CStorage for 24mths at room temperaturePackaging: 5 or 20kg Bags (3 paper and 1 polyethylene layers), and 13kg Plastic Pails.Available for delivery from Brisbane (Australia) or from Atlanta (Georgia)