molecular biology folder
TRANSCRIPT
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IMPORTANT GENE-REGULATION PROTEINS
PINPOINTED BY NEW METHOD
ANDPOLAR GROWTH AT THE
BACTERIAL SCALE REVEALS POTENTIAL NEW TARGETS FOR ANTIBIOTIC
THERAPY.
Laura Maria Másmela gilUniversity medicine student lll semesterJanuary 23, 2012
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INTRODUCTION
A novel technique has been developed and demonstrated at Penn State to map the proteins that read and regulate chromosomes
An international team of microbiologists led by Indiana University researchers has identified a new bacterial growth process , one that occurs at a single end or pole of the cell instead of uniform
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ScienceDaily (Jan. 18, 2012)
PART A
IMPORTANT GENE-
REGULATION PROTEINS
PINPOINTED BY NEW METHOD
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ScienceDaily (Jan. 18, 2012)
PART B
IMPORTANT GENE-REGULATION PROTEINS PINPOINTED BY NEW METHOD
•The new technique precisely pinpoints their location, and has the potential to take high resolution snapshots of proteins as they regulate or miss regulate an entire genome.
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PART C
IMPORTANT GENE-REGULATION PROTEINS PINPOINTED BY NEW METHOD
The research process began by their using a molecular tool called an exonuclease to remove DNA that is not bound by one of the gene-regulating proteins. They then determined the nucleotide sequence for each of the remaining protein-bound DNA bundles.
This technique is known as CHIP-EXO
ScienceDaily (Jan. 18, 2012)
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PART D
Technique CHIP-EXO
Reduce any binding site single nucleotide THROUGH million nucleotides of the
genomes.
provides an unprecedented view of how genes are regulated.
is a low-noise technique that reveals two to five times more binding sites,
which provides a much more complete picture of the genes that are regulated by
a particular protein, and a greater understanding of
their structural organization across genomes .
IMPORTANT GENE-REGULATION PROTEINS PINPOINTED BY NEW METHOD
ScienceDaily (Jan. 18, 2012)
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IMPORTANT GENE-REGULATION PROTEINS PINPOINTED BY NEW METHOD
PERSONAL COMMENT
Its important know about the gene-regulation proteins, because this allows to determine the different type of cells, and a better picture of how the gene pathways work in the normal development of the human body and when a pathology exist.
ScienceDaily (Jan. 18, 2012)
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POLAR GROWTH AT
THE BACTERIAL
SCALE REVEALS
POTENTIAL NEW
TARGETS FOR
ANTIBIOTIC THERAPY.ScienceDaily (Jan. 17,
2012)
PART A
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PART B
ScienceDaily (Jan. 17, 2012)
Based on past detailed studies of rod-shaped bacteria such as Escherichia coli and Bacillus subtilis, it has been assumed that most bacteria grow by binary fission, a dispersed mode of growth involving insertion of new cell wall material uniformly along the long axis of the cell. Growth requires breaking the cell wall at numerous places along the cylinder to allow insertion of new cell wall material, enabling uniform elongation of the cell, with the process culminated by cleavage at the mid-point of the cell to create two symmetric new cells.
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PART C
Outer membrane proteins of an Agrobacterium tumefaciens cell were labeled in red, with images taken every 50 minutes as the cell grew. In panels three and four it is clear that the cell on the left (red) has kept all the labeled proteins, whereas the other cell has all new surface proteins.
Credit: Image courtesy of Indiana University
ScienceDaily (Jan. 17, 2012)
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PART D
using time-lapse microscopy and transmission electron microscopy was observed using four species of bacteria
Polar growth of four bacterial species , the plant symbiont Sinorhizobium meliloti, the plant pathogen Agrobacterium tumefaciens and the human pathogens Brucella abortus
and Ochrobactrum anthropi
these bacteria are members of a class of bacteria called the Alphaproteobacteria.
the results show that polar growth is present in different types of bacteria including types that are outside the classroomalphaproteobacteria.
ScienceDaily (Jan. 17, 2012)
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POLAR GROWTH AT THE BACTERIAL SCALE REVEALS POTENTIAL NEW TARGETS FOR
ANTIBIOTIC THERAPY.
PERSONAL COMMENT
This process of bacterial growth is important because from the molecular biology scientist can do some procedures, the objective is improve the clinical practice , from study cellular method and antibacterial strategies to therapies with organs, systems, and whole human body, preventing diseases.
ScienceDaily (Jan. 17, 2012)
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MEDICAL
UTILITY
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the study of molecular
structures facilitate better
understanding of medicine in their practice.
MEDICAL UTILITY
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MEDICAL UTILITY
investigations allow this type of progress in medical
treatment, as both take preventive measures
on the development of some diseases caused
by specific characteristics of the organisms that had
never been explored in detail. these studies promo
te better health in the human population.
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MEDICAL UTILITY
gene regulatory proteins
allows a more complete vision of the normal and abnormal
human development in the disease
polar growth of bacteria
antibiotic therapy allowed.
PROMOTION AND DISEASE PREVENTION
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Important Gene-Regulation Proteins Pinpointed by New Method.sciencedaily.2012,january 18, from http://www.sciencedaily.com/releases/2012/01/120118132330.htm
Polar Growth at the Bacterial Scale Reveals Potential New Targets for Antibiotic Therapy.sciencedaily.2012,january 17, from http://www.sciencedaily.com/releases/2012/01/120117191421.htm
MARTINEZ S. LINA MARIA, Biología molecular. 6 ed. Medellin; UPB.fac. de medicina 2011, p 10-14;42-55.
BIBLIOGRAFY
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THANKS