mojca brložnik 1 , tomaž curk 2 , klaus natter 3 , uroš petrovič 1
DESCRIPTION
IDENTIFICATION OF GENETIC VARIANTS RESPONSIBLE FOR BIOTECHNOLOGICALLY IMPORTANT TRAITS AND THE DESIGN OF NEW GENERATION INDUSTRIAL YEAST STRAINS. Mojca Brložnik 1 , Tomaž Curk 2 , Klaus Natter 3 , Uroš Petrovič 1 1 Jožef Stefan Institute, Ljubljana, Slovenia. - PowerPoint PPT PresentationTRANSCRIPT
IDENTIFICATION OF GENETIC VARIANTS RESPONSIBLE FOR BIOTECHNOLOGICALLY
IMPORTANT TRAITS AND THE DESIGN OF NEW GENERATION INDUSTRIAL YEAST STRAINS
Mojca Brložnik1, Tomaž Curk2, Klaus Natter3, Uroš Petrovič1
1 Jožef Stefan Institute, Ljubljana, Slovenia.2 University of Ljubljana, Slovenia.
3 University of Graz, Austria.
Fat parentSkinny parent
X
Tiling microarray
Identify the relevant QTLs (SNVs) in pooled strains. Sequencing
Segregants
Average populationExtreme population
QTL#1Extreme population Average population
position position
Fat parent
Skinny parent
Extreme population Average population
QTL#1Tiling microarray
Sequencing
Candidate causative gene
position position
Fat parent
Candidate causative genes:
Single
swaps
Double swaps
Triple swaps
Quadruple swap
CONCLUSIONS
RANDOM MUTAGENESIS
Hundreds - thousands mutants
Robust strain, low productivity
Non-robust strain, high productivity
Identification ofproductivity-related mutations
ALLELESWAPS
Robust strain, high productivity
NEW GENERATION INDUSTRIAL MICROORGANISMS
ACKNOWLEDGEMENTS
Tomaž Curk
Bioinformatics LaboratoryFaculty of Computer and
Information Science University of Ljubljana
Slovenia
Klaus Natter
Yeast Genetics and Molecular Biology Group
Institute of Molecular Biosciences
University of GrazAustria