mir-9

1

0 1 2 4 6 8 10 - -- - - + + + ++ + ++ + ++ + + ++ + ++ + miR-9 0 1 2 4 6 8 10 - - - - - + + + ++ + +- + ++ ++ ++ + ++ + 0 1 2 4 6 8 10 - -- - - + + +- +- -+ + ++ + + ++ + +- + 0 2 4 6 8 10 *36.8% (7/19) Relative expression (/ C20 normal liver) - - - - - + + + ++- -- + ++- + ++ +-- + *33.3% (6/18) *46.2% (6/13) miR-503 Methylation Methylation miR-9-1 miR-9-3 Methylation Methylation Methylation *26.7% (4/15) miR-137 Methylation *0% (0/15) Methylation *7.7% (1/13) miR-149 - -- - - + + - ++ + ++ - -- + + + - + ++ + 12 14 -- -- -- + ++ ++ ++ -- - + +++ ++ ++ 0 1 2 4 6 8 10 miR-196b C20 C40 LC08N LC07N LC11N cHc4 Hep 3B Hep G2 Hep-KANO HLE HLF huH-1 HuH-6 Huh7 JHH-1 JHH-2 JHH-4 JHH-5 JHH-6 JHH-7 Li7 PLC/PRF/5 sK-Hep-1 Hep-TABATA HCC cell lines 0 1 2 4 6 8 10 *35.3% (6/17) + - -- --+ + + + + +- + ++ + + ++ + + - + miR-129 Relative expression (/ C20 normal liver) Supplementary Fig. S1 Relative expression (/ C20 normal liver) Relative expression (/ C20 normal liver) Relative expression (/ C20 normal liver) Relative expression (/ C20 normal liver) Relative expression (/ C20 normal liver) 0 0.2 0.4 0.6 0.8 1.0 1.2 - -- - - + + +++ + +- + ++- + + + +- ++ *75.0% (12/16) miR-596 1.4 1 13.4 12.2 23.4 22.9 14.7 10.3 71.2 11.8 25.2 109 135 556 38.5 55.8 285 394 229 336 23.2 77.4 45.9 35.1 744 56.9 27.7 388 88.7 343 186 92 52 11 21.6 38.8

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Supplementary Fig. S1. 23.4. miR-503. 22.9. 13.4. 12.2. 10. 8. 6. Relative expression (/ C20 normal liver). 4. 2. 1. 0. -. -. -. -. -. +. +. +. +. +. +. +. +. +. +. +. +. +. +. +. +. +. +. +. *36.8% (7/19). Methylation. 71.2. miR-9. 14.7. 11.8. 10.3. - PowerPoint PPT Presentation

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miR-9

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miR-503

Methylation

MethylationmiR-9-1miR-9-3

Methylation

Methylation

Methylation *26.7% (4/15)

miR-137

Methylation *0% (0/15)

Methylation *7.7% (1/13)

miR-149

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HCC cell lines

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miR-129

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Supplementary Fig. S1　

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miR-5961.4

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