microscopy
DESCRIPTION
By Jane Horlings. Microscopy. Microscopy. Robert Hooke, circa 1700s. Electron Microscope, late 1900s. Light Microscopes. Early light microscope (UL), drawing by Hooke (LL). Light Microscopes. Dissecting (stereo) microscope (L), compound microscope (R). Light Microscopy; Plant Cells. - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/1.jpg)
MicroscopyBy Jane Horlings
![Page 2: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/2.jpg)
Microscopy
Robert Hooke, circa 1700s Electron Microscope, late 1900s
![Page 3: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/3.jpg)
Early light microscope (UL), drawing by Hooke (LL)
Light Microscopes
![Page 4: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/4.jpg)
Light Microscopes
Dissecting (stereo) microscope (L), compound microscope (R)
![Page 5: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/5.jpg)
Light Microscopy; Plant Cells
Onion root tip, cell division (L), shoot tip (R)
![Page 6: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/6.jpg)
Light Microscopy, Phase Contrast
Single celled Amoeba dividing (L), green alga Micrasterias (R)
![Page 7: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/7.jpg)
Phase contrast
Fluorescence
Confocal
Light Microscopy
![Page 8: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/8.jpg)
Electron Microscopes
![Page 9: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/9.jpg)
Limitations of Electron Microscopy
• Works in a vacuum• Specimens are dead,
chemically preserved; no life processes can be seen
• No color (colorized by artist on computer)
![Page 10: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/10.jpg)
Electron MicroscopesTransmission Electron Microscope (TEM)
Scanning Electron Microscope (SEM)
![Page 11: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/11.jpg)
Scanning Electron Microscopy
SEM of cilia
TEM of cilia
![Page 12: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/12.jpg)
Bacterium
RBCs in clot
SEM
![Page 13: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/13.jpg)
Mite (UL)
Gecko toes (LL)
Shark skin (LR)
SEM
![Page 14: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/14.jpg)
Red blood cells and Trypanosoma (L), Giardia (R)
SEMTrypanosome
Red blood cells
![Page 15: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/15.jpg)
Transmission Electron Microscopy
SEM of cilia
TEM of cilia
![Page 16: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/16.jpg)
Viruses (L), animal cell (R)
TEM
![Page 17: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/17.jpg)
Animal cell (L), muscle tissue (R)
TEM
![Page 18: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/18.jpg)
Light Microscopy
• Based on light• Specimens can be
alive; life processes can be seen
• Color; dyes may be used
![Page 19: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/19.jpg)
Parts of the Microscope
• Ocular lenses
• Objective lenses
• How to compute the magnification
![Page 20: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/20.jpg)
![Page 21: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/21.jpg)
Use of the Microscope
• Place slide in center• Adjust light, lenses, barrel
– Put on low magnification!– Move stage all the way up
and then back down half a turn!
![Page 22: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/22.jpg)
Use of the Microscope
• Look and readjust focus
• Move to higher magnification if needed
![Page 23: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/23.jpg)
Use of the Microscope
• Importance of focusing with the fine adjustment!
• Oil immersion lens
• How to adjust the light
![Page 24: Microscopy](https://reader037.vdocuments.us/reader037/viewer/2022110210/56812b77550346895d8f9861/html5/thumbnails/24.jpg)
End