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MicroscopyBy Jane Horlings
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Microscopy
Robert Hooke, circa 1700s Electron Microscope, late 1900s
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Early light microscope (UL), drawing by Hooke (LL)
Light Microscopes
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Light Microscopes
Dissecting (stereo) microscope (L), compound microscope (R)
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Light Microscopy; Plant Cells
Onion root tip, cell division (L), shoot tip (R)
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Light Microscopy, Phase Contrast
Single celled Amoeba dividing (L), green alga Micrasterias (R)
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Phase contrast
Fluorescence
Confocal
Light Microscopy
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Electron Microscopes
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Limitations of Electron Microscopy
• Works in a vacuum• Specimens are dead,
chemically preserved; no life processes can be seen
• No color (colorized by artist on computer)
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Electron MicroscopesTransmission Electron Microscope (TEM)
Scanning Electron Microscope (SEM)
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Scanning Electron Microscopy
SEM of cilia
TEM of cilia
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Bacterium
RBCs in clot
SEM
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Mite (UL)
Gecko toes (LL)
Shark skin (LR)
SEM
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Red blood cells and Trypanosoma (L), Giardia (R)
SEMTrypanosome
Red blood cells
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Transmission Electron Microscopy
SEM of cilia
TEM of cilia
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Viruses (L), animal cell (R)
TEM
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Animal cell (L), muscle tissue (R)
TEM
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Light Microscopy
• Based on light• Specimens can be
alive; life processes can be seen
• Color; dyes may be used
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Parts of the Microscope
• Ocular lenses
• Objective lenses
• How to compute the magnification
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Use of the Microscope
• Place slide in center• Adjust light, lenses, barrel
– Put on low magnification!– Move stage all the way up
and then back down half a turn!
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Use of the Microscope
• Look and readjust focus
• Move to higher magnification if needed
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Use of the Microscope
• Importance of focusing with the fine adjustment!
• Oil immersion lens
• How to adjust the light
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End