Precursor Clone Collection

System Biosciences (SBI) has the largest

commercially-available collection of

microRNAs that are cloned into lentiviral

vectors. SBI’s constructs can be stably

expressed in a wide variety of cell types,

as opposed to synthetic microRNAs that

can only be transiently tested in cells.

Each construct in SBI’s collection consists

of the native stem loop structure and 200-400 base pairs of upstream and downstream � anking genomic

sequence. This unique feature ensures that the microRNAs expressed from SBI’s constructs will be correctly

processed in the cell into mature microRNAs.

Droshaprocessing

miR : miR*

dsRNA Intermediate

miR* separation

5’ 3’

5’3’

5’3’ 5’

3’

3’ 5’

miRISC complexes

DicerAGO2

AmpR

3’∆LTRWPRE

copGFPSV40 poly-A

cPPT

SV40 ORI

pUC ORI

RSV

CMV

EF1

5’LTRΨ

Dicerprocessing

microRNAPrecursors

microRNA Research

Lenti-miR™ microRNAPrecursor ClonesOverexpress Individual microRNAs or Pooled Virus Library

Highlights

• Largest collection of microRNA precursor clones available in lentivectors

• Native microRNA context ensures accurate and robust mature microRNA production

• Confi rm positive expressing cells with copGFP for convenient sorting of transfected or transduced cells

• Easily create stable cell lines

• High-throughput microRNA phenotypic screens using pooled Lenti-miR virus library

The microRNA precursors expressed from SBI’s Lenti-miR vectors produce mature microRNAs via the host cell’s processing machinery.

www.systembio.com/lentimir

5’ 3’

Native genomic sequences �anking hairpin (~400 bp)

Native microRNAhairpin structureretained.

SBI’s microRNAprecursor clonesare the real thing.

MCF-7 Cells infected (MOI 5:1) with Lentivirus made from PMIRH205PA-1 (pre-miR-205) construct. Images taken after 48 hours. Expression of mature miR-205 was validated and quantitated using qPCR assays.

0

2

4

6

8

10

12

miR-205 Control

Rela

tive

Expr

essi

on L

evel

(Nor

mal

ized

to U

6)

Mature miRNA Expression

MCF-7 Breast Cancer CellsTransduced with Lenti-miR-205

Phase Contrast GFP Fluorescence

Lentiviral Pre-miR-205 transduction

Lenti-miR™ microRNA Precursor Clones

Product Description Catalog # Size

MicroRNA Precursor Pooled Library(Pre-packaged) >10^7 IFUs/aliquot

PMIRHPLVA-1 4 virus aliquots

MicroRNA Precursor Pooled Library(HT, Pre-packaged) >10^7 IFUs/aliquot

PMIRHPLVAHT-1 10 virus aliquots

Pooled Lentivirus Particles of all SBI’s MicroRNA Precursors

Screen All microRNAs Simultaneously for Unique Phenotypes

SBI’s Lenti-miR virus library contains the pooled Lenti-miR precursor

clone collection in a ready-to-infect lentiviral preparation. The library

is designed for studying phenotypic e� ects associated with the over-

expression of individual microRNAs in hard-to-transfect mammalian

cell lines, primary cells, non-dividing cells and even whole animal

studies. The lentivirus preparation is pseudotyped with VSV-G for

broad cellular tropism. The microRNA or microRNAs responsible for

generating the phenotypes of interest may be recovered through

simple genomic PCR using lentivector-specifi c primers followed by

direct sequencing of microRNA precursor clones recovered.

Perform One Transduction and Easily Identify themicroRNAs Involved in Your Phenotypic Screen

Each virus within the pool will express an individual microRNA

precursor in its native context while preserving hairpin structures

to ensure biologically relevant interactions with endogenous

processing machinery and regulatory partners.

Identi�cation of microRNA E�ectors

Treatmentto inducephenotype

Selectionfor phenotype

Ampli�cationof microRNAe�ectors fromselected cells

InfectedTarget Cells

Treated Cells

Tumor InvasionCell Di�erentiationApoptosis

Transductionwith Lenti-miRmicroRNA Library

Pooled Lentiviral microRNA Screens

Simple Genomic PCR to Identify microRNA E�ectors

MicroRNA Precursor cloneCMV

5’ LVL Primer 3’ LVL Primer

copGFP

MicroRNAs

1000

500

(bp)M 16 29a 210 155 651 203 34a 125b

GFP

Flu

ores

cenc

ePh

ase

Cont

rast

HEK 293 Cells HT1080 Cells

Lentivirus Infection QS Data

We Also O� er Custom ServicesSystem Biosciences o� ers a wide-range of custom services to support your research, allowing you to spend less time making tools, and more time making discoveries. To learn more, visit our website at www.systembio.com/service or call us at 888-266-5066.

© 2009 System Biosciences, Inc. All rights reserved. System Biosciences and the System Biosciences logo are trademarks of System Biosciences, Inc.

System Biosciences, Inc.1616 North Shoreline Blvd.Mountain View, CA 94043

Toll Free: 888.266.5066Fax: 650-968-2277Email: info@systembio.comwww.systembio.com

The most comprehensive microRNA Precursor Clone Collection in lentiviral vectors available anywhere.