michael john mclane - slide holder
TRANSCRIPT
Dual Slide IHC Staining ChamberMichael McLane
3D model credit: Jon Bondy (Vermont Rapid Prototyping)
“The Team”
• Michael McLane– UVM undergraduate (CALS: Biological Science), Class of
2016; works in UVM Kraft and Jetton Laboratories
• Tom Jetton– Ph.D., UVM Department of Medicine: Endocrinology,
Diabetes, and Metabolism: Professor
• Jon Bondy– Vermont Rapid Prototyping: 3D design, 3D printing
IMMUNOHISTOCHEMISTRY(Fancy way of saying “staining with antibodies”)
Problems with microscope slide-based IHC:
Why should we care?• Disease research on almost any condition you can think of will often
involve slide-based IHC
• Ex.: Jetton/Kraft Lab (14-047) currently investigating Type 2 diabetes in mouse models– I stain pancreatic tissue sections for levels of key β-cell factors
• Academic labs must typically conduct these IHC methods by hand
• Large portion of laboratory budget goes towards antibodies; conserving grant money is important
• Failure due to error can result in loss of critical data
How can methods be improved?
• Stains two slides per chamber with 2/3 the amount ofreagents typically used to cover two slides lying face-up
• Much faster / easier than previous methods– Lowers likelihood of data loss due to human error
• Easier storage during incubation periods
• Should improve staining quality
“Dual Slide IHC Staining Chamber:”
How I can use IMF / Funding:• 3D printing using chemically inert, sturdy plastics
– Possibility of rubber siding for better fit & seal?– Add a cap for humidity seal
• Branch out beyond IHC:
– In situ hybridization techniques?– Oil Red O techniques?– Radiographic emulsions?– More…?
• Other uses for $:
– Testing supplies– Time on confocal microscope for data collection (+ subsequent analysis comparing chamber staining to
original methodology)