1994

METHoDs

" ' : " * edition

• cfl~Js t(ai~or ~US~r~ MiChaefj~ Aamn M~tchel!

- L o Methods in Yeast Genetics A Cold Spring Harbor Laboratory Course Manual, 1994 Edition By Chris Kaiser, Massachusetts Institute of Technol- ogy; Susan Michaelis, Johns Hopkins Medical School; Aaron Mitchell, Columbia University Col- lege of Physicians & Surgeons

The Cold Spring Harbor Yeast Genetics Course, founded in 1970, evolves continuously to present a combination of standard methods and the latest tech- niques in yeast biology. This is the latest edition of the course manual, which incorporates a variety of new techniques with revised experiments and the es- sential elements from the 1987 and 1990 publica- tions. The book remains the primary source of techni- cal guidance for anyone using yeast, either as a new- comer or as an established investigator.

C O N T E N T S Introduction Genetic Nomenclature Experiments Looking at Yeast Cells; I. Isolation and Characterization of Auxotrophic, Temperature-sensitive, and UV-sensitive Mutants; II. Meiotic Mapping; III. Mitotic Recombination: Loss of Heterozygosity and Mitotic Mapping; IV. Trans- formation of Yeast; V. Complementation and Random Spore Analysis of Histidine Auxotrophs; VI. Gene Re- placement; VII. Isolation of ras2 Suppressors; VIII. The Formation of Zygotes: Mating and Karyogamy; IX. Clon- ing of Yeast Genes by Complementation; X. lacZ Gene Fu- sion Expression in Yeast; XI. lmmunofluorescent Staining

of Yeast Cells; XII. Electrophoretic Karyotyping and Gene Mapping Using Pulsed-field Gel Eiectrophoresis Techniques and Protocols 1. Yeast Transformation; 2. Lithium Acetate Yeast Trans- formation; 3. Yeast Colony Hybridization; 4. Yeast DNA Isolations: a. Yeast DNA Miniprep (40 ml), b. Yeast DNA Miniprep (5 ml), c. A Ten-minute DNA Prep from Yeast, d. Preparation of Yeast Genomic DNA (CsCi Method); 5. Yeast Protein Extracts; 6. Yeast RNA Isolation; 7. Northern Analysis: Formaldehyde Agarose Gel, Blotting, and Hybridization to Filters; 8. Alkaline Southern Blotting Pro- cedure; 9. Scoring Killer Factor; 10. Hydroxylamine Mutagenesis of Plasmid DNA; 11. Assay of I~-Galacto- sidase in Yeast; 12. Transformation of Bacteria (CaC! 2 Method); 13. E. coli Plasmid DNA Miniprep; 14. Prepara- tion of Chromosome-sized Yeast DNA Molecules in Solid Agarose; 15. Mapping Cloned DNA Segments by Chromo- some "Fragmentation"; 16. Running Gels Using the CHEF Apparatus; 17. Running Gels Using the OFAGE Apparatus; 18. Yeast Vital Stains; 19. Yeast lmmunofluorescence with Antibodies; 20. Actin Staining in Fixed Cells; 21. Modified Lithium Acetate Yeast Transformation; 22. PCR Analysis of Genotype Appendices A. Media; B. Stock Preservation; C. Yeast Genetic Map; D. Grids; E. Electrophoretic Karyotypes of Strains for Southern Blot Mapping; F. Strains

1994, 202 pp., illus., appendices ISBN 0-87969-451-3 Plastic comb binding $49

Reader Service No. 316

/geubl:e Tal/ g H.:¢lix F lu-.es " . . . the point o f this story, I'II tell you right now.

Did you ever sit down and think about how

It is that each time a baby's born

It 's a baby - - not a rabbit or an ear o f corn ?"

The answer is in DNA m

the s tu f f that genes are made of.

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Written by Joe: Herskowi tz .

I l lus t ra ted by J u d y Cuddihy . A d d i t i o n a l text by Ira Herskowi t z .

1993, Cloth (plus 12-minute audio cassette), ISBN 0.87969.431.9:$20

hiuS delightful tape-book package is nique way of learning about DNA

and genes and how they work. On tape, hear identical twin brothers

Joel and Ira Herskowitz each perform the Double Talking Helix Blues. Same song, same genes, different styles.

In the book, read through the lyrics and enjoy Judy Cuddihy's beautiful illustrations. Chromosomes and cells really do look like this, just a bit less colorful.

And on the back pages, be entertained and intrigued by a guide to heredity that makes all the details clear.

The Double Talking Helix Blues is interesting and fun for young people (8 and up) and adults who are curious about how they and their relatives became the unique individuals they are.

About the authors and illustrator:

Joel Herskowitz lives in Framingham, Massachusetts where he practices pediatric neurology. A graduate of Princeton University (as a music major), he is on the staff of New England Medical Center Hospitals in Boston. The "Double Talking Helix Blues" was originally written for

O Cold Spring Harbor

L a b o r a t o r y Press

"fa[ t g H::.dis iblucs Three easy ways to order.* Call: 1-8001843-4388 (Continental U.S. and Canada)

516/349-1930 (All other locations) Fax: 516/349-1946 Write: CSHL Press, 10 Skyline Drive, Plainview, NY 11803

Printed on recycled & recyclable paper and printed using soy-based inks. @

his father, geneticisi Irwin H. Herskowitz, who is Professor Emeritus of Biology at Hunter College in the City University of New York.

Ira llerskowitz, Joel's identical twin brother (and 7 minutes older), is professor and chairman of the Department of Biochemistry and Biophysics at the University of California, San Francisco. He is a member of the National Academy of Sciences and has been recognized for his scientific contributions to molecular genetics by a MacArthur Foundation Fellowship and the Genetics Society of America Award. He has performed the "Double Talking Helix Blues" for students and at lectures throughout the country.

Judy Cuddihy is an editor with Cold Spring Harbor Laboratory Press. She graduated from Bucknell University with a degree in biology and has studied at the Parsons School of Design in New York City.

Reader Service No. 317