Manipulating the Mous Embryo A Laboratory Manual, Second Edition By Brigid Hogan, Vanderbilt University Medical School; Rosa Beddington, National Institute for Medical Research, London; Frank Costantini, Columbia University; Elizabeth Lacy, Memorial Sloan-Kettering Cancer Center

The 1986 publication of Manipulating the Mouse Embryo catalyzed the interaction between molecular biology and mammalian embryology. For the first time, detailed instructions on how to begin applying recombinant DNA technology to important questions about mammalian embryonic development were made available to a broad audience. The gather- ing pace of such studies in recent years has brought improvements to existing methods and fueled the creation of new and powerful technologies. The sec- ond edition of this classic manual has been complete- ly revised and expanded to incorporate these ad- vances. It contains new sections on the production and analysis of transgenic mice, the manipulation of preimplantation embryos to generate chimeras, the culture and manipulation of embryonic stem cells, in- eluding gene "knockouts," and techniques for visual- izing genes, gene products, and specific cell types. As before, included with the protocols is a summary of current understanding of mouse development at a molecular level. In its new edition, this manual of proven distinction is again an authoritative and com- prehensive source of technical guidance for experi- enced investigators and an essential resource for new- comers to mammalian genetics and embryology.

CONTENTS Developmental Genetics and Embryology of the Mouse: Past, Present, and Future Section A. Summary of Mouse Development Section B. Setting Up a Colony for the Production of Transgenic and Mutant Mice

Section C. Recovery, Culture, and Transfer of Embryos and Germ Cells Section D. In Vitro Manipulation of Preimplantation Embryos Section E. Production of Transgenic Mice Section F. Isolation, Culture, and Manipulation of Embryonic Stem Cells Section G. Analysis of Transgenic Mice Section H. Techniques for Visualizing Genes, Gene Products, and Specialized Cell Types Section I. In Vitro Culture of Eggs, Embryos, Primordial Germ Cells, and Teratocarcinoma Cells Appendices Suggested Reading References

1994, 497 pp., illus., color plates, appendices, glossary index Plastic comb binding $95 ISBN 0-87969-384-3 Cloth $190 ISBN 0-87969-392-4

Reader Service No. 318

GENES

DEVELOPMENT

VOLUME 9 NUMBER 4 PAGES 387-508 FEBRUARY 15, 1995

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Contents GENES & DEVELOPMENT February 15, 1995

Research papers

The Cpx two-component signal transduction pathway of Escherichia coil regulates transcription of the gene specifying the stress-inducible periplasmic protease, DegP Paul N. Danese, William B. Snyder, Christine L. Cosma, Laura J.B. Davis, and Thomas J. Silhavy

387

G~ cyclin turnover and nutrient uptake are controlled by a common pathway in yeast Yves Barral, Stefan Jentsch, and Carl Mann

399

Reciprocal interferences between nucleosomal organization and transcriptional activity of the yeast SNR6 gene Marie-Claude Marsolier, Shigeo Tanaka, Magda Livingstone-Zatchej, Michael Grunstein, Fritz Thoma, and Andr6 Sentenac

410

Identification and characterization of genes that are required for the accelerated degradation of mRNAs containing a premature translational termination codon Ying Cui, Kevin W. Hagan, Shuang Zhang, and Stuart W. Peltz

423

Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen Feng He and Allan Jacobson

437

DNA rearrangements located over 100 kb 5' of the Steel (S/)-coding region in Steel-panda and Steel-contrasted mice deregulate SI expression and cause female sterility by disrupting ovarian follicle development Mary A. Bedell, Camilynn A. Brannan, Edward P. Evans, Neal G. Copeland, Nancy A. Jenkins, and Peter I. Donovan

455

Inhibition of viral gene expression by the catalytic RNA subunit of RNase P from Escherichia coli Fenyong Liu and Sidney Altman

471

RNA polymerase II subunit RPB9 is required for accurate start site selection Melissa W. Hull, Keith McKune, and Nancy A. Woychik

481

flbD encodes a Myb-like DNA-binding protein that coordinates initiation of Aspergillus n/du/ans conidiophore development Jenny Wieser and Thomas H. Adams

491

Cell--cell signaling pathway activating a developmental transcription factor in Bacillus subtilis J.-Arturo Londofio-Vallejo and Patrick Stragier

503

Cover Expression of Mgf mRNA in P12 ovaries SlP~n/S1 p~n mice. (For details, see Bedell et al., p. 455.)