method to expose rats to ozone-updated2014

Exposing Rats to Ozone – Method Setup

Update 2014

There are other ways to do this much cheaper, but this would put together since this what was freely available at the time. Today I would probably use an Arduino or some other cheap datalogger with simple relay capability. There are too many vendors to list.

For ozone monitoring, the 2B Technologies Model 202 Ozone Monitor would do fine. It is EPA approved as well. It has the capability of built in storage card and has an analog output.

It might also be a good idea to have a small fan and some heating strips to circulate the air and minimize moisture from the rats' breathing. It is also good to minimize any moisture to prevent damageto the ozone monitor.

It was never necessary, but it would have been neat to have swapped the potentiometer control for ozone level with an electronic one. Otherwise, it is a test and go until it is right at first.

Make sure generator and ozone monitor are sized appropriately to the concentrations and size of the enclose area.

A Crystal Air (or the slightly bigger model)

http://www.ozone.ca/products/DC-PRO-200.htm

Ozone Monitor

http://www.twobtech.com/model_202.htm

Crystal Air


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http://www.twobtech.com/model_202.htm


Purpose: This document explains the basics of setting up an experimental system for exposing mice or rats to high concentrations of ozone. Most of the components may be available over the internet from lab suppliers or other vendors.

The setup for exposing the rats to ozone consists of a closed chamber, a Dasibi 1008 series ozone monitor, a Zeno 3200 datalogger from Coastal Environmental Systems, a dehumidifier for the chamber,a couple of heating strips for regulating temperature/humidity in the chamber, and a Crystal Air Model 450 ozone generator.

The heating strips, dehumidifier, and ozone monitor need to operate constantly. Air is fed into the chamber from the laboratory compressed air supply. A sampling tube is inside the chamber and attachesto the ozone monitor. The ozone monitor has an output voltage that is fed into a channel on the Zeno 3200 datalogger. The datalogger records the ozone level in one minute averages and activates a 12V relay that controls a standard 110V outlet to turn the ozone generator on or off. The ozone level is programmed into the datalogger and the datalogger records the ozone levels and determines when the relay should run the ozone generator on or off with some simple if/then combinations that are part of the Zeno 3200's operating system. A desktop or laptop computer is available only to access the datalogger with Window's Hyperterminal software to download the data and program the datalogger.

Materials and Equipment

Glove Box: This chamber also has power strip built-in. One power strip or electrical cord will have to be committed for turning the ozone generator on and off. The heating strips and dehumidifier should beleft on all the time. This will probably require another power strip. It will also be important to have constant supply of lab compressed air flowing into the chamber so the animals do not suffocate.

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Dasibi 1008 AH Ozone Monitor

This particular model and brand of ozone monitor is no longer made. Other models can be used. Make sure to keep moisture out of these monitors. Some models do not tolerate condensation too well and one could end up with some expensive repairs.

Zeno 3200 Datalogger

http://www.coastalenvironmental.com/Coastal Environmental Systems, Inc.820 First Avenue SouthSeattle, WA 98134

Phone:206-682-6048 800-488-8291Fax: 206-682-5658

This model has a numerous channels for recording electrical signals from ozone monitors to ph meters or wind speed or thermocouples. It also has some general programming capability so electrical relays can be activated at certain times or when a specific value has been reached by a recording instrument. Itfunctions independently of a desktop or laptop computer.

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http://www.coastalenvironmental.com/


12V Relay

Radioshack12VDC/10A DPDT Plug-in Relay Model: 275-218 | Catalog #: 275-218

Wiring Diagrams/Schematics

Materials: 12V Relay, some small gauge wire of a few inches or more (used to wire the relay and ozone monitor voltage output to datalogger), an electrical cord, a standard outlet with 2 plugs, a box and cover to house the outlet and relay.

The standard electrical cord of 12 or 14 gauge wire will typically have a black, white, and green. One can also cut off the end of an extra computer cord. Solder the black wire and white wire to the prongs as indicated on the relay. Wire the green ground wire directly to the connection on the plug. Solder a n inch or two from the relay and strip the ends of the wire to connect the outlet to the relay. Most plugs usually have a copper and silver screw and a ground connection; wire the black to copper and the whiteto silver. This outlet will be used to turn the ozone generator on and off.

Wire the relay to the 12V connections on the datalogger; wire one to Channel A, B, or C and the other to a ground (any of the channels will work, just make sure to modify programming accordingly). Wire the the output of the ozone monitor to the channel on the datalogger; wire the (+) to the input channel and the (-) to a ground connection on the datalogger terminals.

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Zeno Program

Follow this if inputting the setting manually. The program to upload is at the end of this document. Thisis a crude control system, so it may be necessary to adjust the values plus or minus at the desired point. For example, if 800 ppb is desired, the program might be set to 801 or 805 ppb before cutting off the ozone generator. Keep in mind that the datalogger is an electronic device and it will always DO WHAT YOU TELL IT TO DO not what you want it to do.

* Zeno 3200 System Configuration Menu File* Program Version And Date: ZENO-3200 using ZENOSOFT V2.02 Sep 10 2002 11:29:41 CS B97B* (C)opyright 1995-2002, Coastal Environmental Systems, Seattle, WA, USA.* Menu Setup File Date And Time: 07/07/25 16:09:41

COMMUNICATIONS MENU(Cn/m) Change Item n To Value m (R) Repeater Menu (M) Modem Menu (Tn) Terminal Mode On COM Port n(P) Power Control Menu (E) Save Parameters To EEPROM (G) GOES Menu (U) User Menu (A) ARGOS Menu (Q) Quit (D) Digital Control Menu (H) Help

Item 1: 9600 (COM1 Baud Rate)Item 2: 9600 (COM2 Baud Rate)Item 3: 9600 (COM3 Baud Rate)Item 4: RS232 (COM1 Port Type)Item 5: RS232 (COM2 Port Type)Item 6: RS232 (COM3 Port Type)

SYSTEM FUNCTIONS MENU(Cn/m) Change Item n To Value m (I) Contact Information (S) System Date And Time (E) Save Parameters To EEPROM(T) Calibrate Internal Temperature (U) User Menu (V) Program Version (Q) Quit (K) Constants Menu (H) Help (B) BIT Names Menu

Item 1: 0 (Primary Unit/Experiment ID)Item 2: 0 (Secondary Unit/Experiment ID)Item 3: 1 (Data Dump Format)Item 4: 1 (Real Time Output Format)Item 5: 0 (Add Compass To Vane)Item 6: 0 (Compass Offset)Item 7: 0 (Barometer Elevation)Item 8: (Bad Sensor Value Replace)Item 9: <ESC> (Passthrough Mode Escape Character)

SAMPLE PERIOD MENU(Cn/m) Change Item n To Value m (Q) Quit(E) Save Parameters To EEPROM (H) Help(U) User Menu

Item 1: 60 (Sample Interval Time)

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Item 2: 30 (Sample Duration Time)Item 3: 0 (Sample Time Offset)

MODEM MENU(Cn/m) Change Item n To Value m (U) User Menu(E) Save Parameters To EEPROM (Q) Quit (S) Speech Interface Menu (H) Help

Item 1: 0 (System Modem Type)Item 2: NONE (Modem COM Port)Item 3: 5 (Modem Retry Period)Item 4: 0 (Modem Hangup Delay)Item 5: NONE (Modem Telephone Number 1)Item 6: NONE (Modem Telephone Number 2)Item 7: NONE (Modem Telephone Number 3)Item 8: NONE (Modem Telephone Number 4)Item 9: NONE (Modem Initialization String)Item 10: 0 (Modem Switched Voltage)Item 11: 0 (Modem Ring Detect Channel)

POWER CONTROL MENU(Cn/m) Change Item n To Value m (Q) Quit(E) Save Parameters To EEPROM (H) Help(U) User Menu

Item 1: 100 (COM1 Push-To-Talk Setup Time)Item 2: 0 (COM1 Switched Power Code)Item 3: 0 (COM2 Switched Power Code)Item 4: 0 (COM3 Switched Power Code)Item 5: 0 (Response Delay Time (milliseconds)

GOES MENU(Cn/m) Change Item n To Value m (E) Save Parameters To EEPROM(D) Run GOES Diagnostics (U) User Menu (R) Reset GOES Errors (Q) Quit (I) Initialize GOES (H) Help

Item 1: 03001190 (Data Collection Platform Address)Item 2: 151 (Self-Timed Transmit Channel Number)Item 3: 00:03:00:00 (Self-Timed Transmission Interval)Item 4: 00:00:00 (Self-Timed Transmission Offset)Item 5: 1 (Transmission Window Length)Item 6: SHORT (Satellite Link Parameter: Preamble)Item 7: 151 (Random Transmit Channel Number)Item 8: 00:00:00 (Random Transmission Interval)Item 9: 00:05:00 (Random Disable Time)Item 10: 1200 (GOES Bit Rate)Item 11: NONE (GOES Interleaver)

DIGITAL CONTROL MENU(Cn/m) Change Item n To Value m (Q) Quit(E) Save Parameters To EEPROM (H) Help(U) User Menu

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Item 1: 000:00:00 (Power On Interval)Item 2: 000:00:00 (Power On Duration)Item 3: 03/07/29 15:00:00 (Start of First Power On)Item 4: 0 (Control Channel)

REPEATER MENU(Cn/m) Change Item n To Value m (U) User Menu(R) Reset All Repeater Values (Q) Quit (E) Save Parameters To EEPROM (H) Help

Item 1: Repeater Tranmit Delay 0 msecItem 2: Network Source Address -1Item 3: Alternative Source Address -1Item 4: Remote ZENO Address #1 -1Item 5: Remote ZENO Address #2 -1Item 6: Remote ZENO Address #3 -1Item 7: Remote ZENO Address #4 -1Item 8: Remote ZENO Address #5 -1Item 9: Remote ZENO Address #6 -1Item 10: Remote ZENO Address #7 -1Item 11: Remote ZENO Address #8 -1

ARGOS MENU(Cn/m) Change Item n To Value m (U) User Menu(E) Save Parameters To EEPROM (Q) Quit (D) Display ARGOS Message (H) Help

Item 1: 42 (Tranmission Repeat Interval, seconds)Item 2: 1 (Transmission Repeat Count)Item 3: 10 (Radio Push-To-Talk Delay Time, milliseconds)Item 4: 0 (Radio Push-To-Talk Digital Port)Item 5: NO (Use Hour/Min Time Stamp in Message)Item 6: NO (Use Checksum in Final Message Byte)

CONSTANTS MENU(Cn/m) Change Constant n to Value m (E) Save Parameters To EEPROM(P) Previous Page of Constants (U) User Menu (N) Next Page of Constants (Q) Quit (X) Erase All Constants (H) Help

Constants 1 through 20Constant 1: 0 Constant 11: 0Constant 2: 100 Constant 12: 0Constant 3: 0 Constant 13: 0Constant 4: 0 Constant 14: 0Constant 5: 0 Constant 15: 0Constant 6: 0 Constant 16: 0Constant 7: 0 Constant 17: 0Constant 8: 0 Constant 18: 0Constant 9: 0 Constant 19: 0Constant 10: 0 Constant 20: 0

SPEECH INTERFACE MENU

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(Cn/m) Change Item n To Value m (Q) Quit(E) Save Parameters To EEPROM (H) Help(U) User Menu

Item 1: NO (Speech Interface Installed)Item 2: 0 (Speech Activity Channel)Item 3: 0 (Speech Tone Detect Channel)Item 4: 0 (Speech Switched Voltage)

BIT NAMES MENU(Cn/m) Change Bit n to Value m (Q) Quit(E) Save Parameters To EEPROM (H) Help(U) User Menu

BIT Names 1 through 32Bit 1: ZENO-3200-Reset Bit 17: Bit 2: Real-Time-Clock-Suspect Bit 18: Bit 3: Logging-Memory-Initialized Bit 19: Bit 4: Serial-Sensor-COM-Failure Bit 20: Bit 5: EEPROM-Suspect Bit 21: Bit 6: 18-Bit-ADC-Suspect Bit 22: Bit 7: 12-Bit-ADC-Suspect Bit 23: Bit 8: Temperature-Clock-Adjustment Bit 24: Bit 9: Bit 25: Bit 10: Bit 26: Bit 11: Bit 27: Bit 12: Bit 28: Bit 13: Bit 29: Bit 14: Bit 30: Bit 15: Bit 31: Bit 16: Bit 32:

BACK DOOR MENU(Cn/m) Change Item n To Value m (G) Reinitialize Flash Memory(F) Calculate Free Heap Memory (X) Display Stack Usage (A) Auto-Calibrate Compass (E) Save Parameters To EEPROM(I) Initialize Compass (W) Harware Test Menu (R) Reset Parameters To Defaults (U) User Menu (Y) Configuration Heap Usage (H) Help

Item 1: 16777 (Processor Clock Speed)Item 2: 0 (RAM/ROM Wait States)Item 3: 60 (50/60 Hz Rejection For 18-bit ADC)Item 4: 18 (13/18 Bit Operation Of 18-bit ADC)Item 5: VOLTS (A To D Conversion Results)Item 6: NO (Expert Menu Mode)Item 7: 32767.69 (Real-time Clock Crystal Frequency At 25 Degrees C)Item 8: 0 (Speed vs. Noise Tradeoffs For 18-bit ADC, Factory Only)Item 9: 1.0000 (12-bit ADC Correction Factor)Item 10: 0 (Zero Offset Period in Seconds)Item 11: 500 (Low Freq. Sensor Timeout in msec)

SENSOR MENU(Cn/m) Change Item n To Value m (Jn) Jump To Record n

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(A) Insert After This Record (N) Go To Next Record (B) Insert Before This Record (P) Go To Previous Record(X) Cut Record to Clipboard (XA) Delete ALL Records (C) Copy Record To Clipboard (Z) Zeno Program Menu (V) Paste Record From Clipboard (H) Help (Sn/m) Search Item n for Value m

Sensor Items for Record 1 of 3:Item 1: Sensor Type Code 3 (18-bit Differential A to D)Item 2: Sensor Name pHSTItem 3: Sensor Input Channel 7Item 4: Analog Channel Gain 1Item 5: Analog Channel Attenuation 1Item 6: Switched Power Code 3 (12V SW'D A)Item 7: Sensor Excitation Voltage Code 0 (NO EXCITATION VOLTAGE)Item 8: Switched Excitation Return 0Item 9: Switched Power Warmup Time 3Item 10: Sensor Sample Count 1Item 11: Maximum Sensor Readings 0Item 12: Sensor Timing Loop 2 (1.0 seconds)Item 13: Conversion Coefficient A 0Item 14: Conversion Coefficient B 40Item 15: Conversion Coefficient C 0Item 17: No. of Additional 15-msec Delays 0

Sensor Items for Record 2 of 3:Item 1: Sensor Type Code 3 (18-bit Differential A to D)Item 2: Sensor Name pHMTItem 3: Sensor Input Channel 3Item 4: Analog Channel Gain 1Item 5: Analog Channel Attenuation 1Item 6: Switched Power Code 3 (12V SW'D A)Item 7: Sensor Excitation Voltage Code 0 (NO EXCITATION VOLTAGE)Item 8: Switched Excitation Return 0Item 9: Switched Power Warmup Time 3Item 10: Sensor Sample Count 1Item 11: Maximum Sensor Readings 0Item 12: Sensor Timing Loop 2 (1.0 seconds)Item 13: Conversion Coefficient A 0Item 14: Conversion Coefficient B 40Item 15: Conversion Coefficient C 0Item 17: No. of Additional 15-msec Delays 0

Sensor Items for Record 3 of 3:Item 1: Sensor Type Code 3 (18-bit Differential A to D)Item 2: Sensor Name condItem 3: Sensor Input Channel 5Item 4: Analog Channel Gain 1Item 5: Analog Channel Attenuation 1Item 6: Switched Power Code 3 (12V SW'D A)Item 7: Sensor Excitation Voltage Code 0 (NO EXCITATION VOLTAGE)Item 8: Switched Excitation Return 0

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Item 9: Switched Power Warmup Time 3Item 10: Sensor Sample Count 1Item 11: Maximum Sensor Readings 0Item 12: Sensor Timing Loop 2 (1.0 seconds)Item 13: Conversion Coefficient A 0Item 14: Conversion Coefficient B 40Item 15: Conversion Coefficient C 0Item 17: No. of Additional 15-msec Delays 0

PROCESS MENU(Cn/m) Change Item n To Value m (Jn) Jump To Record n (A) Insert After This Record (N) Go To Next Record (B) Insert Before This Record (P) Go To Previous Record(X) Cut Record to Clipboard (XA) Delete ALL Records (C) Copy Record To Clipboard (Z) Zeno Program Menu (V) Paste Record From Clipboard (H) Help (Sn/m) Search Item n for Value m

Process Items for Record 1 of 5:Item 1: Process Category 5 : ArithmeticItem 2: Process Number 8 : Quadratic ProcessItem 3: User-defined Process Name pHSTItem 4: Input for Data (X) S1 : pHSTItem 5: A2 Coefficient 0Item 6: A1 Coefficient 1.113Item 7: A0 Coefficient -6.148

Process Items for Record 2 of 5:Item 1: Process Category 5 : ArithmeticItem 2: Process Number 8 : Quadratic ProcessItem 3: User-defined Process Name pHMTItem 4: Input for Data (X) S2 : pHMTItem 5: A2 Coefficient 0Item 6: A1 Coefficient 0.98Item 7: A0 Coefficient -4.522

Process Items for Record 3 of 5:Item 1: Process Category 5 : ArithmeticItem 2: Process Number 8 : Quadratic ProcessItem 3: User-defined Process Name CondItem 4: Input for Data (X) S3 : condItem 5: A2 Coefficient 0Item 6: A1 Coefficient 694.5Item 7: A0 Coefficient -2777.8

Process Items for Record 4 of 5:Item 1: Process Category 3 : Alarm

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Item 2: Process Number 3 : Alarm ProcessItem 3: User-defined Process Name pHch3Item 4: Input for Data S2 : pHMTItem 5: Alarm Setpoint 12Item 6: Alarm Reset 13.5Item 7: BIT Flag Number (1 - 31) 0Item 8: Digital Output Channel 1 0Item 9: Digital Output Channel 2 0Item 10: Switched Voltage 4

Process Items for Record 5 of 5:Item 1: Process Category 3 : AlarmItem 2: Process Number 3 : Alarm ProcessItem 3: User-defined Process Name condItem 4: Input for Data S3 : condItem 5: Alarm Setpoint 6.5Item 6: Alarm Reset 6.4995Item 7: BIT Flag Number (1 - 31) 0Item 8: Digital Output Channel 1 0Item 9: Digital Output Channel 2 0Item 10: Switched Voltage 5

DATA OUTPUT MENU(Cn/m) Change Item n To Value m (Jn) Jump To Record n (A) Insert After This Record (N) Go To Next Record (B) Insert Before This Record (P) Go To Previous Record(X) Cut Record to Clipboard (XA) Delete ALL Records (C) Copy Record To Clipboard (Z) Zeno Program Menu (V) Paste Record From Clipboard (H) Help (Sn/m) Search Item n for Value m

Data Items for Record 1 of 5:Item 1: Field Type Code 9 : Transmit and Log Data FieldItem 2: Output Message(s) 1Item 3: Field Name pHmASTItem 4: Input Record S1 : pHSTItem 5: Field Decimal Places 1Item 6: Field Width 6Item 7: Data Storage Class Code 8 : 4-byte floating point

Data Items for Record 2 of 5:Item 1: Field Type Code 9 : Transmit and Log Data FieldItem 2: Output Message(s) 1Item 3: Field Name pHSTItem 4: Input Record P1.1 : pHSTItem 5: Field Decimal Places 1Item 6: Field Width 6Item 7: Data Storage Class Code 8 : 4-byte floating point

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Data Items for Record 3 of 5:Item 1: Field Type Code 9 : Transmit and Log Data FieldItem 2: Output Message(s) 1Item 3: Field Name condSTItem 4: Input Record P3.1 : CondItem 5: Field Decimal Places 1Item 6: Field Width 6Item 7: Data Storage Class Code 8 : 4-byte floating point

Data Items for Record 4 of 5:Item 1: Field Type Code 9 : Transmit and Log Data FieldItem 2: Output Message(s) 1Item 3: Field Name pHmAMTItem 4: Input Record S2 : pHMTItem 5: Field Decimal Places 1Item 6: Field Width 6Item 7: Data Storage Class Code 8 : 4-byte floating point

Data Items for Record 5 of 5:Item 1: Field Type Code 9 : Transmit and Log Data FieldItem 2: Output Message(s) 1Item 3: Field Name pHMTItem 4: Input Record P2.1 : pHMTItem 5: Field Decimal Places 1Item 6: Field Width 6Item 7: Data Storage Class Code 8 : 4-byte floating point

GENERAL SERIAL SCRIPT MENU(Cn/m) Change Line n to m (X) Delete this Script (In) Insert Line n (Jn) Jump to Script n (Rn) Remove Line n (N) Go to Next Script (En) Erase Line n (P) Go to Previous Script(L) Delete All Lines (XA) Delete All Scripts (A) Insert After this Script (Z) Zeno Program Menu (B) Insert Before this Script (H) Help

Script Lines for Script 1 of 1Line 1: NO_COMMAND

SENSOR TIMING LOOP MENU(Cn/m) Change Item n To Value m (H) Help(Z) Zeno Program Menu

Item 1: 0.5 (Timing Loop #1 Period)Item 2: 1.0 (Timing Loop #2 Period)Item 3: 1.5 (Timing Loop #3 Period)Item 4: 2.0 (Timing Loop #4 Period)

OUTPUT MESSAGE TIMING MENU(Cn/m) Change Item n To Value m (H) Help(Z) Zeno Program Menu

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Item 1: COM3 (Data Output Message #1 COM Port)Item 2: COM3 (Data Output Message #2 COM Port)Item 3: COM3 (Data Output Message #3 COM Port)Item 4: COM3 (Data Output Message #4 COM Port)Item 5: 0.0 (Data Output Message #1 Period)Item 6: 0.0 (Data Output Message #2 Period)Item 7: 0.0 (Data Output Message #3 Period)Item 8: 0.0 (Data Output Message #4 Period)Item 9: 0 (Record Counter Starting Value)Item 10: 0 (Record Counter Reset Value)

MEMORY MANAGEMENT MENU(Cn/m) Change Item n To Value m (H) Help(Z) Zeno Program Menu

Item 1: 0 (COM1 GSI Memory Size in Bytes)Item 2: 0 (COM2 GSI Memory Size in Bytes)Item 3: 0 (COM3 GSI Memory Size in Bytes)* !!SYSTEM TRANSFER COMPLETE.* Turn Off File Capture Now.* Enter Any Key To Continue.EOF

Copy this to a text editor like Notepad and upload the file to the Zeno 3200. Make sure the top line is the beginning of the file with no extra spaces:

* Zeno 3200 System Setup File* Program Version And Date: ZENO-3200 using ZENOSOFT V2.02 Sep 10 2002 11:29:41 CS B97B* (C)opyright 1995-2002, Coastal Environmental Systems, Seattle, WA, USA.* Setup File Date And Time: 07/07/25 16:09:19PARAM1 60 0 30 2 3 4 0 0 9600 9600 PARAM2 9600 0 0 0 0 1 1 0 0 0 PARAM3 16777 0 60 18 0 0 0 0 2 2 PARAM4 2 2 0 0 0 3276769 0 -1 5 0 PARAM5 3 0 0 0 100 0 0 0 0 0 PARAM6 0 0 0 1059577200 50336144 151 196608 0 1 0 PARAM7 151 0 1280 0 10000 -1 -1 0 10 1 PARAM8 42 0 0 0 0 0 0 0 0 0 PARAM9 0 0 0 0 0 0 5 0 0 0 PARAM10 500 0 0 0 1200 -1 27 PARAM11 "NONE" "NONE" "NONE" "NONE" "NONE" "NONE" "" "ZENO" "" "NONE" PARAM12 "" "ZENO-3200-Reset" "Real-Time-Clock-Suspect" "Logging-Memory-Initialized""Serial-Sensor-COM-Failure" "EEPROM-Suspect" "18-Bit-ADC-Suspect" "12-Bit-ADC-Suspect" "Temperature-Clock-Adjustment" "" PARAM13 "" "" "" "" "" "" "" "" "" "" PARAM14 "" "" "" "" "" "" "" "" "" "" PARAM15 "" "" "" REPEAT1 -1 -1 -1 -1 -1 -1 -1 -1CONSTANT1 0 100 0 0 0 0 0 0 0 0 CONSTANT2 0 0 0 0 0 0 0 0 0 0 GSI 1 NO_COMMANDSENSOR 3 "pHST" 7 0 0 3 0 0 3 1 0 2 0 40 0 0 0 0 0 0 0 0 0 0 S0.1

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SENSOR 3 "pHMT" 3 0 0 3 0 0 3 1 0 2 0 40 0 0 0 0 0 0 0 0 0 0 S0.1 SENSOR 3 "cond" 5 0 0 3 0 0 3 1 0 2 0 40 0 0 0 0 0 0 0 0 0 0 S0.1 PROCESS 5 8 "pHST" S1.1 0 1.113 -6.148 PROCESS 5 8 "pHMT" S2.1 0 0.98 -4.522 PROCESS 5 8 "Cond" S3.1 0 694.5 -2777.8 PROCESS 3 3 "pHch3" S2.1 12 13.5 0 0 0 4 PROCESS 3 3 "cond" S3.1 6.5 6.4995 0 0 0 5 DATA 9 1 "pHmAST" S1.1 1 6 8 P1.1 P1.1 P1.1 DATA 9 1 "pHST" P1.1 1 6 8 P1.1 P1.1 P1.1 DATA 9 1 "condST" P3.1 1 6 8 P1.1 P1.1 P1.1 DATA 9 1 "pHmAMT" S2.1 1 6 8 P1.1 P1.1 P1.1 DATA 9 1 "pHMT" P2.1 1 6 8 P1.1 P1.1 P1.1 * !!SYSTEM TRANSFER COMPLETE.* Turn Off File Capture Now.* Enter Any Key To Continue.EOF

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Heat Strip

Dehumidifier and heat strips inside chamber.

Ozone Monitor

OzoneGenerator

Relay and outlet wired together inside box. Control wires go to chosen 12V channel on datalogger. The ozone value is read from the ozone monitor to turn the ozone generator on and off.

110VAC

Fan. Circulates air in chamber and shows that circuit is turned on.

(+) and (-) for output voltage of monitor.

method to expose rats to ozone-updated2014

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