metabolomics. strategic approach for gc amenable compounds
DESCRIPTION
Metabolomics is the newest hype in the 'omics' family. Although defined as highly important to recent biological research, the analytical science applied is far from acceptable for the majority of publications that appear nowadays. Take a look at our approach to tackle the metabolomics issue, which remains a new name for an old science.TRANSCRIPT
METABOLOMICSA strategic approach for GC amenable compounds
Interscience Expert Centerwww.is-x.be
Analysis of small-molecule metabolites formed by the cell in association with its metabolism.
“
Accurate reflection of changes in cell functions taking genotype and phenotype into account.
Bacteria
Food quality assessmentEnvironmental remediation
Antibiotics discovery
Plants
Stress assessment studies Identification of gene function
Cultivar differentiation
Mammals
Health status assessmentPrediction of clinical outcomes
Patient classification
Glycomics
Lipidomics
Flavouromics
Environmental
metabolomics
Emerging spin-offs
Renewed hype for an ‘old science’ Classical biochemical conceptsClassical analytical techniques
Metabolite screening
“Generic approach to detect & identify all metabolites in a given cell or organism”
Metabolite fingerprinting
“Identify and compare chromatographic patterns in response to change”
Metabolite profiling
“Detect & quantify a class of compounds in a given cell or organism”
Metabolite target analysis
“Detect & quantify a limited and specific set of compounds in a given cell or organism”
Approximately 85% of the articles published in ‘Metabolomics’ apply non-target screening.
Carolyn Honour, Editorial Director ‘Metabolomics’ (Springer)
Unfortunately,
the non-target screening approach suffers from some serious drawbacks.
Extremely complex
matrices
High sample variability
Large chemical diversity
Target analysis
Profiling
Screening
“ Accuracy
Sco
pe
Scientifically most relevant approach…
… true target analysis in combination with data from other ‘omics’ experiments.
Method requirements
Correct sample preparation
Accurate analysis
Fast data handling
Sample preparation
Too often the bottleneck…
Go for automated solutions that apply ‘state-of-the-art’ sampler robotics.
Target analysis
High throughput required…
Go for GC in combination with the selectivity of MS/MS in h-SRM mode.
Data management
Go for automated data reporting solutions with direct LIMS interfacing.
Conclusion
Expert chromatographic knowledge is mandatory for you to decide which strategy to apply so you can be sure that the results you report are scientifically correct.
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