localized and automated chemical and oxygen delivery system for microfluidic brain slice devices
DESCRIPTION
Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices. Author: Gene Yu Co-Authors: Dr. AlexBlake Dr. David Eddington July 29, 2010 NSF Research Experiences for Undergraduates (REU) in - PowerPoint PPT PresentationTRANSCRIPT
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Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices
Author: Gene Yu Co-Authors: Dr. AlexBlake
Dr. David EddingtonJuly 29, 2010
NSF Research Experiences for Undergraduates (REU) inNovel Advanced Materials and Processing with Applications in Biomedical, Electrical and
Chemical Engineering
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ObjectiveDesign localized and
automated delivery system for microfluidic brain slice devices (μBSDs)
Delivers chemicals/drugs and gases
Automatically mixes oxygen
Characterize delivery of chemicals through tissue Image taken from
Mohammed, J, Caicedo, H, Fall, C, & Eddington, D. (2008). Microfluidic add-on for standard electrophysiology chambers. Lab Chip, 8, 1048-1055.
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MotivationμBSDs allow in vitro study of:
NeurogenesisSynaptogenesisRegenerationProtein expressionResponses to physical trauma
Image taken fromhttp://www.koki.hu/cdnb/research/research.html
Rambani, K, Vukasinovic, J, Glezer, A, & Potter, S. (2009). Culturing thick brain slices: an interstitial 3d microperfusion system for enhanced viability. Journal of Neuroscience Methods, 180, 243-254.
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RelevanceChemical Delivery
Spatial resolution Current systems affect entire
slice with drug Desire stimulation of specific
areasOxygen Delivery
Automatic Mixing Saves money Pre-mixing is not required
AutomationMulti-tasking
Image modified fromhttp://www.imagingeconomics.com/issues/articles/MI_2006-08_01.asp
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Oxygen Mixing and DeliveryExperimental Set-Up
Y Connector
Output Tube
Valves Gas Feed Lines: 0% and 21%
Oxygen concentration measured by a NeoFox Fiber Optic Oxygen (FOXY) sensor
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Oxygen Mixing and DeliveryOutput
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 210
4
8
12
16
20
Oxygen Output
Desired Oxygen Levels (%)
Ave
rage
Exp
eri-
men
tal O
xyge
n Le
v-el
s (%
)
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Oxygen Mixing and DeliveryGraphical User Interface (GUI)
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Delivery CharacterizationμBSD Design
T Channel
Delivery Channel
Reservoir
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Delivery CharacterizationExperimental Set-up
μBSD
Valve
DI Water Lines T Channel
Vacuum Line
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Delivery CharacterizationNo Tissue Delivery
10ms 15ms 20ms 25ms
2x5ms 3x5ms 4x5ms 5x5ms
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Delivery CharacterizationLinear Relationships: No Tissue
Width (µm)
Width (µm)
860
1320
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Delivery CharacterizationLinear Relationships: With Tissue
Full Dose Chemical Delivery
Pulsing Dose Chemical Delivery
0 0.5 1 1.5 2 2.5 3 3.5 4 4.50
20406080
100120140160
f(x) = 19.750471 x + 1.3889974R² = 0.97197294218466
Maximum Intensities
0 2 4 6Number of 5ms Pulses
Inte
nsity
Inte
nsity
Inte
nsity
Time (min)
Time (min)
2
2
0 5 10 15 20 25 300
20406080
100120140
f(x) = 4.904786 x + 12.66823R² = 0.898041669777912
Maximum Intensities
0 10 20
30Valve Open Time (ms)
Inte
nsity
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Delivery CharacterizationGUI
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ConclusionsOxygen Delivery
Precise and accurate results
Reliable oxygen switching
Chemical Delivery Demonstrated linear
relationship Area of effect: ~2mm x
2mm Selective and
independent valve control
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AcknowledgementsFunding
NSF-REU DoD-ASSURE EEC-NSF Grant # 0755115
REU Directors Christos G. Takoudis, Ph.D. Greg Jursich, Ph.D.
Research Advisor David Thomas Eddington, Ph.D.
Mentors Alexander Blake, Ph.D. Gerardo Mauleon