list of posters - altex 2013 altex proceedings 2, 2/13, linz 2013 ix list of posters in alphabetical...

Linz 2013

ALTEX Proceedings 2, 2/13, LINZ 2013 IX

List of postersIn alphabetical order of the first authors

S. Ayehunie, Z. Stevens, T. Landry, A. Armento, M. Klausner, and P. HaydenA New Organotypic 3-D Human Small Intestinal Tissue Model Reconstructed from Primary Human Cells 4

S. Ayehunie, C. Hedin, T. Landry, M. Spratt, M. Clark, T. Kupper, and M. KlausnerNovel 3-D reconstructed human tissues model of psoriatic skin 5

M. Bartok, D. Gabel, M. Engelke, M. Zorn-Kruppa, J. M. Brandner, K. Reisinger, K. Daton, and K. MewesHuman 3D corneal models for a detailed quantification of the initial depth of injury as an indicator for cellular damage in the human eye 6

M. D. Brauneis and P. SteinbergCoupling of the BALB/c-3T3 cell transformation assay to a metabolic activation system and to the soft agar colony formation assay: determination of two endpoints in a single in vitro test system 9

K. Brown and R. HarrisonThe Lush Prize – Supporting Animal Free Testing 10

F. Caloni, A. Theodoridis, V. Kehagias, C. Cortinovis, F. Pizzo, and M. SachanaAlternative methods in education: what do students think? 13

S. Commandeur, S. Sparks, L. van Zijl, M. Rietveld, H. J. Lai, C. C. Lin, and A. El GhalbzouriOlogen® Collagen Matrix: a new dermal scaffold for skin tissue engineering 15

S. Corke, G. Foss-Smith, K. Hewitt, L. E. Haswell, D. Azzopardi, and G. PhillipsInvestigation into the inflammatory response of the H292 lung epithelial model to cigarette smoke particulate generated using different smoking regimes and reduced toxicant prototypes 16

T. Doktorova, G. Ates, C. Chesne, T. Vanhaecke, and V. RogiersThe way forward in case of a false positive in vitro genotoxicity result for a cosmetic substance? 20

A. Érseková, J. Klánová, J. Giesy, and J. NovakIn vitro toxicology of air samples: Breathe or not to breathe? 21

A. C. Feutz and C. de GeyterTesting neurodevelopmental toxicity on differentiating human embryonic stem cells 23

D. Fieblinger, D. Barthel, J. Tharmann, K. Maul, S. Trappe, M. Götz, and A. LuchGenotoxicity of nanosized TiO2 particles in human reconstructed full thickness skin models 24

B. Filipič, G. Lidija, M. Hrvoje, M. Slobodan, and S. KorenThe antiproliferative, proapoptototic and antitumor activity of huifn-α against CaCo-2 cells can be enhanced with 10% PBS holocene grain wash out 25

B. Filipič, A. Pereyra, M. Hrvoje, E Šooš, J. Potokar, and S. KorenHuIFN-αN3 inducing capacity of the Newcastle disease virus (NDV) ZG1999HDS 26

E. Fritsche, K. Gassmann, S. Giersiefer, J. Schuwald, and J. BaumannNeurospheres as a predictive 3D in vitro model for DNT testing and pathway investigation in a species-specific context 29

altex_2013_Linz-I.indd 9 26.8.2013 15:51:35 Uhr

Linz 2013

ALTEX Proceedings 2, 2/13, LINZ 2013X

J. GoodmanUse of Animals and Alternatives in Military Medical Training by NATO Nations 31

M. Gotz, K. Maul, D. Barthel, D. Fieblinger, D. Storm, A. Köth, D. Wittke, A. Luch, and M. GötzGenotoxicity of nanosized Titanium dioxide particles in 16HBE14o- cells 32

K. Guth, J. Riviere, J. Brooks, M. Schaefer-Korting, M. Dammann, E. Fabian, B. van Ravenzwaay, and R. LandsiedelIn silico models for dermal absorption from complex formulations 35

T. Hasenberg, E. M. Materne, C. Frädrich, U. Süßbier, R. Horland, S. Hoffmann, S. Brincker, A. Lorenz, M. Busek, F. Sonntag, R. Lauster, and U. MarxDynamic culture of human liver equivalents inside a micro-bioreactor for long-term substance testing 36

P. Hayden, R. Jackson, J. Bolmarcich, and M. KlausnerTissue engineered in vitro human airway models (Epiairway) of asthma and COPD 37

P. Hinterdorfer and L. ChtcheglovaMolecular Recognition Force Microscopy/Spectroscopy 39

M. C. Inglez de Souza, A. A. Ribeiro, and J. M. MateraImproving cadaveric models for surgery teaching and training 41

N. Jukes and D. LeporskyInterNICHE outreach and replacement of animal experiments in education in Uzbekistan and Kyrgyzstan 43

C. Julius, D. Lehmeier, I. Schultz, and A. AlbrechtA 3R test of biocompatibility of a new intensive patch by using the reconstituted three-dimensional human skin model EPISKIN-SM™ (SKINETHIC) to replace animal use 44

Y. Kaluzhny, M. W. Kinuthia, P. Hayden, L. d’Argembeau-Thornton, H. Kandarova, and M. KlausnerIn Vitro Phototoxicity Screening Assay for Systemically Administered Pharmaceuticals Using a Reconstructed Skin Model EpiDerm 45

Y. Kaluzhny, H. Kandarova, L. d’Argembeau-Thornton, J. De Luca, P. Hayden, A. Hunter, T. Truong, and M. KlausnerOptimization of the EpiOcular Eye Irritation Test for Hazard Identification and Labelling of Chemicals in Response to the Requirements of the EU Cosmetic Directive and REACH Legislation 46

H. Kandarova, S. Letasiova, T. Milasova, P. Hayden, and M. KlausnerAnalysis of the Validated Epiderm Skin Corrosion Test (EpiDerm SCT) and a Prediction Model for Sub-Categorization According to the UN GHS and EU CLP 47

S. Y. Kim, Y. K. Lee, M. H. Nam, C. H. Lim, J. Y. Yang, I. Y. Ahn, and K. H. ChoiStudy on development for the alternative methods for photo-toxicity and photo-genotoxicity tests using mouse keratinocytes and human 3D skin model 51

S. Kimeswenger, K. A. Vincze-Minya, K. R. Schröder, S. Hild, and M. R. Lornejad-SchäferConfocal µ-Raman spectroscopy of living cells 52

S. N. Kolle, A. Mehling, N. Honarvar, W. Teubner, B. van Ravenzwaay, and R. LandsiedelAlternative Method in practice: postvalidation experience of the skin sensitization in vitro test strategy 58

P. Kosina, A. Galandáková, S. Snášelová, and J. UlrichováIn vitro toxicity testing: influence of different cell lines and endpoints 59

E. Koutsouraki, S. Pells, and P. De SousaThe role of dioxygenases in human embryonic stem cells 60


Linz 2013

ALTEX Proceedings 2, 2/13, LINZ 2013 XI

E. Koutsouraki and P. De SousaDefining compound subcytotoxic effects on epigenetic determinants of human embryonic stem cell renewal and lineage commitment 61

J. Lenoir, I. Claerhout, P. Kestelyn, J. P. Remon, and E. AdriaensUsing the Slug Mucosal Irritation assay to predict discomfort caused by ophthalmic formulations 66

S. Letasiova, H. Kandarova, M. Bachelor, P. Kearney, and M. KlausnerImportance of reproducibility demonstration of the bio-engineered tissue models used for in vitro toxicity testing purposes 67

M. Lewis and J. M. WilkinsonMoving forward: a new paradigm for drug discovery 68

L. J. Löwenau, S. Wattanapitayakul, J. M. Brandner, G. Weindl, and M. Schaefer-KortingHuman epidermis reconstructed from UVB-irradiated keratinocytes mimics premature ageing in human skin 73

J. Madden, A. Richarz, D. Neagu, C. Yang, E. Fioravanzo, A. Pery, A. Worth, M. Berthold, and M. CroninThe COSMOS Project - Developing Integrated Computational Approaches to Predict Repeated Dose Toxicity 75

V. Marashi, N. Alzmann, and H. GrimmAnimal Testing and Transparency – a Contradiction in Terms? Presenting a Project to Promote both, Transparency of Animal Testing and the Social Dialogue between Proponents and Opponents 76

V. Marashi, N. Alzmann, and H. Grimm„Taking Ethical Considerations Into Account? Methods to Carry Out the Harm-Benefit Analysis According to the EU Directive 2010/63/EU“. Summary of a Symposium at the Messerli Research Institute 77

U. Markert, A. Schmidt, C. Göhner, J. Pastuschek, and M. WeberThe human placenta in toxicology 78

T. Markovic, M. Gobec, D. Gurwitz, and I. Mlinaric-RascanCharacterization of lymphoblastoid cell lines as a novel in vitro test system to predict immunotoxicity of xenobiotics 79

K. Maul, D. Fieblinger, A. Heppenheimer, J. Kreutz, A. Luch, R. Pirow, A. Poth, K. Reisinger, P. Strauch, and T. WolfResults of a (pre)validation study of the Hen’s Egg Test for Micronucleus-Induction (HET-MN) 81

K. McQuillanUsing 21st Century toxicology to model disease risk 82

K. Meganathan, S. Jagtap, S. Perumal Srinivasan, V. Shindev, V. Wagh, J. Hescheler, and A. SachinidisHuman Embryonic Stem Cells Differentiation Reveals Toxicity Signatures for HDAC inhibitors and Mercuric Toxicants 83

F. Noor3D Organotypic cultures of liver cells for metabolism and toxicity testing 85

O. O'Shea, L. Healy, and G. StaceyQualification of neural differentiation protocols and points to consider when transferring standardized protocols between laboratories 86

R. OwensA novel, flexible method for assessing barrier tissue integrity 87

G. Pallocca, B. Zimmer, N. Dreser, S. Foerster, N. Balmer, S. Julien, K. H. Krause, S. Bosgra, and M. LeistScreening of a large group of medical substances and environmental pollutants in a neural crest stem-cell based functional migration assay 88


Linz 2013

ALTEX Proceedings 2, 2/13, LINZ 2013XII

M. Paparella, M. Daneshian, R. Hornek-Gausterer, M. Kinzl, I. Mauritz, and S. MühleggerUncertainty of testing methods – what do we (want to) know? 89

S. Pells, E. Koutsouraki, S. Morfopoulou, S. Valencia-Cadavid, A. Malinowski, R. Riddoch, S. Tomlinson, R. Kalathur, M. Futschik, and P. De SousaEpigenetically-conserved biomarkers of an undifferentiated human embryonic stem cell phenotype 90

T. Ramirez Hernandez, T. Weisschu, H. A. Huener, B. van Ravenzwaay, and R. LandsiedelNeurotoxicity in vitro: assessment of the predictivity of neuronal networks coped to microelectrode arrays for identification of neurotoxicants 93

C. Rauch, E. Feifel, and G. GstraunthalerHuman Platelet Lysates Successfully Replace Fetal Bovine Serum in Adipose-derived Adult Stem Cell Culture 95

C. Rauch, E. Feifel, A. Flörl, K. Pfaller, and G. GstraunthalerHuman Platelet Lysates Promote the Differentiation Potential of Adipose-derived Adult Stem Cell Cultures 96

J. Reich, W. Mutter, and H. GrallertEvaluation of two new recombinant Factor C based assays as alternatives for Limulus blood based endotoxin detection methods 97

R. Roshanaie and N. JukesHumane education and ethical science: Campaigning for replacement in Iran 98

C. RovidaHow many animals have been really used for REACH purposes: appraisal after the second deadline 99

C. Rovida and M. AquinoDialogue with EFSA to avoid useless animal testing in the area of genotoxicity 100

M. Rucki, K. Kejlova, and D. JirovaAcute toxicity of alcohols determined by alternative methods 101

C. Schäfer, M. R. Lornejad-Schäfer, and K. R. SchröderA human liver 3D cell model for substance testing 105

C. Schäfer, W. Hilber, K. R. Schröder, B. Jakoby, and M. R. Lornejad-SchäferConstruction of an Impedance monitoring system for cell seeding and drug screening in a 3D cell culture model 106

A. Seiler, K. Hayess, C. Riebeling, R. Pirow, M. Steinfath, D. Sittner, B. Slawik, K. Gulich, and A. LuchThe DNT-EST: a predictive embryonic stem cell test for developmental neurotoxicity testing in vitro 111

A. Sharanek, P. Bachour-El Azzi, C. Guguen-Guillouzo, and A. GuillouzoAlterations of canalicular and basolateral transporters by cyclosporin A in human HepaRG® cells 113

K. Sommer, E. Bradt, S. Constant, D. Breheny, and K. R. SchröderNext Generation Sequencing (NGS) approaches to detect fixed point mutations in human airway epithelia 115

G. StoddartREACH – Who is responsible for ensuring that animal testing is conducted only as a last resort? 117

L. Stoppini, A. Sandoz, and I. CharvetDevelopment of a microfluidic biochip for chronic monitoring of 3D neural tissues derived from human Embryonic Stem Cells 118


Linz 2013

ALTEX Proceedings 2, 2/13, LINZ 2013 XIII

M. Talikka, J. Hoeng, J. Binder, S. Boué, V. Belcastro, A. Iskandar, E. Bilhal, P. Meyer Rojas, R. Norel, J. J Rice, K. Rhrissorrakrai, J. Park, J. Sprengel, Fields, W. Hayes, R. Kleiman, M. Peitsch, and G. StolovitzkyVerification of Systems Biology Research in the Age of Collaborative- Competition 119

K. TaylorReview of REACH from an animal protection perspective 121

K. TaylorA ‘low toxicity profile’ can waive the 90-day repeated dose test for REACH 122

K. TaylorThe harm:benefit assessment under the new Directive 2010/63- will any projects be rejected? 123

A. M. VinggaardAre structural analogs to bisphenol A a safe alternative? 127

I. Wagner, B. Atac, G. Lindner, F. Sonntag, R. Lauster, and U. Marx“Skin on a Chip” - Perfused long term culture of skin tissue 128

D. Walter, M. Niehof, A. Hackbarth, and T. HansenStudies on the mode of action of Multi-walled Carbon Nanotubes onto human bronchial epithelial cells 129

G. Weindl, N. Do, M. Salwiczek, B. Koksch, and M. Schaefer-KortingTopical application of cationic membrane-active peptides: enzymatic degradation by human skin ex vivo and the effect on skin penetration 131

D. Weisensee, O. Engelking, T. Klein, D. Fuchs, and H. FuchsEvaluation of the sub-classification of dermal corrosives in vitro using the epiCS® (CellSystems) reconstructed human skin model 133

R. A. WessNon-testing strategies - a tiered approach 134

J. Wiest, R. Kolar, and I. RuhdelComparison of standard and fetal-calf-serum-free cell culture media by impedance measurement 136

J. Wiest, A. Steininger, and T. LindlMonitoring of multilayer development of human 3D cornea constructs by trans-epithelial impedance measurement 137

D. Zagoura, F. Pistollato, J. Louisse, S. Colleoni, G. Lazzari, A. Sachinidis, and S. BremerElucidation of perturbed pathways by using stem cell derived neural progenitors 138

S. Colleoni, C. Galli, 2, J. A. Gaspar, K. Meganathan, S. Jagtap, J. Hescheler, S. Bremer, A. Sachinidis, and G. LazzariDevelopment of a neural teratogenicity test: comparative microarray analysis of two different hESCs lines following exposure to valproic acid 138

M. Zorn-Kruppa, M. Bartok, D. Gabel, M. Engelke, K. Reisinger, K. Daton, K. Mewes, and J. M. BrandnerDevelopment and characterization of a bioengineered conjunctiva model on the basis of immortalized cells 139

C. Zoschke, N. Alnasif, E. Fleige, R. Haag, H. F. Merk, G. Weindl, S. Küchler, and M. Schaefer-KortingIn Vitro Non-Melanoma Skin Cancer Model for Toxicity Testing and Pre-Clinical Drug Evaluation 140


Linz 2013

ALTEX Proceedings 2, 2/13, LINZ 2013XIV


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