let your lc inlet do the work - waters … your lc inlet do the work for your nano- to microscale...

[ ACQUITY UPLC M-CLASS SYSTEM ]

LET YOUR LC INLET DO THE WORK FOR YOUR NANO- TO MICROSCALE SEPARATIONS

Analytical scientists are constantly

challenged to increase the sensitivity

of their LC/MS analyses to meet the

demanding goals of their laboratories.

In order to achieve this, focus is typically

directed toward improving the sensitivity

of the mass spectrometer or decreasing

the diameter of the LC column. Adapting

conventional separations instruments can

cause problems with reproducibility and

reliability. These questions of usability

prevent many scientists from taking

full advantage of nano- to microscale

separations in their methods.

Over the past 10 years UPLC® Technology

has continued to provide improved

chromatographic performance.

Now, with Waters’ newest addition, the

holistically designed ACQUITY UPLC®

M-Class System, nano- to microscale

separations will never be the same.

The ACQUITY UPLC M-Class System is

the most flexible nano- to microscale

instrument available to deliver UPLC-

quality separations across a wide range

of column dimensions, providing the

sensitivity required for an array of

applications. The system provides excellent

performance over the range of column

sizes, from 75 μm to 1.0 mm, delivering

outstanding results over a 170-fold

range of column volumes.

IF YOU’RE NOT T RYING TO INC REASE SENSIT IV IT Y BY UPGRADING YOUR LC INLET, YOU HAV E NO IDEA W HAT YOU’V E BEEN MISSING

desiredthe analyses you always

As a member of the ACQUITY UPLC

family, holistic design and intuitive

diagnostics make the ACQUITY UPLC

M-Class System the most robust,

reliable, and flexible UPLC system for

nano- to microscale separations.

With unprecedented usability, the

ACQUITY UPLC M-Class System

enables laboratories to analyze

compounds in low concentrations

amid complex matrices.

Confidently achieve your laboratory

goals with high-efficiency ACQUITY UPLC

M-Class Columns ranging from 75 µm

to 1.0 mm I.D. Use complementary mass

spectrometry to support our full range of

column I.D.’s, or run 300 µm to 1.0 mm

columns with optical detection.


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ESI+ MSMicro-probe9 μL/min

CaffeineLidocaineSulfadimethoxineTerfenadineDanazol

BEH, 300 µm x 150 mm 5% to 95%B14 μL/min

14,000 psiACQUITY UPLC M-Class

8,000 psinanoACQUITY UPLC

Achieve faster separations using higher flow rates.

In this small molecule analysis the ACQUITY UPLC M-Class System provides a faster separation by approximately 30% when compared to the nanoACQUITY UPLC System.

T HE MOST ROBUST, RELIABLE, HIGHEST PERFORMING UPLC SYST EM FOR NANO- TO MIC ROSCALE SEPARAT IONS

■■ Higher sensitivity for any MS

■■ Maximum amount of data

from any sample

■■ Potential for less sample

preparation volume and cost

■■ Reduced solvent consumption

■■ Measure small amounts of analyte

in complex matrices with 2D

■■ Flexible configurations,

scalability, and confidence

for your laboratory

Run a wider range of column dimensions

for wide-ranging applications

desired

The ACQUITY UPLC M-Class System is

the product of years of Waters’ resolute

commitment to providing a reliable

nano- to microscale system. The low

system volume and newly redesigned

fluidics minimize dispersive and

adsorptive losses, ensuring superior

chromatographic resolution through

efficient use of sub-2-µm particles.

The system features improved robust

fittings, columns, and valves to ensure

that your analyte is resolved and

delivered to your mass spectrometer

or optical detector for characterization

and quantification experiments.

The ACQUITY UPLC M-Class System

features a microscale ESI probe in

the ZSpray™ API interface to deliver

superior sensitivity. As column I.D.

is decreased, this micro-MS probe

reduces band-broadening to maintain

chromatographic integrity.

T HE ACQUIT Y UPLC M-CLASS SYST EM DELIV ERS:

confidentlysee morewith less

■■ Highest sensitivity

UPLC separations yield increased

analyte concentration for higher

UV and MS signal responses

■■ Maximum chromatographic

resolution

New 15k psi-capable

ACQUITY UPLC M-Class

Columns deliver sub-2-µm

particle performance

■■ Faster nano- and

microscale separations

Increased operating pressures

yield maximum flexibility for

higher throughput analyses

■■ Higher resolution with

longer columns

Increased pressure enables

more separation power

■■ Confidence in sample analysis

New non-reactive wetted surfaces

throughout the fluidic path minimize

absorptive losses

■■ Increased solvent compatibility

Improved range of solvent

blends precisely metered

for gradient fidelity

■■ Increased sample capacity

Sample organizer compatibility

allows you to store a wide variety

of plates and vials in the system

for longer, unattended operation

■■ UV compatibility

UV flow cells compatible with

300 µm to 1.0 mm I.D. columns to

augment your UPLC/MS analysis

■■ Scalability

With flow rates from 200 nL/min

to 100 μL/min, achieve the best

chromatographic performance,

from 75 µm to 1.0 mm I.D. columns

covering a 170-fold range of

column volumes

■■ Increased flexibility

More valve configurations

enable a variety of applications,

increased throughput, and

maximized peak capacity


The most reliable and

highest performing

nano- to microscale

instrument available

to deliver true UPLC

separations across the

widest range of sample

and column sizes.

■■ Maximum reproducibility

Direct flow control

■■ Interactive on-board

diagnostics

Effectively monitor

system performance

■■ MassLynx® Software

Fully compatible with

the system and the

suite of data processing

Application Managers

ACQUITY UPLC M-Class System with ACQUITY TUV Detector.

innovations that provide flexibility

Engineered for the lowest dispersion –

including an improved ESI micro-probe

design – and with an extended pressure

envelope, complex separations can

be accelerated without compromising

chromatographic fidelity.

The ACQUITY UPLC M-Class System is the

result of years of Waters’ experience and

research and delivers the promise of

robust nano- to microscale separations.

It was designed to meet the expanding

needs of researchers – every aspect

of the system is directed toward

increasing information.

Optical and MS detection

Optical detection grants an additional

identification and quantification tool

orthogonal to mass spectrometry.


maximizes peak capacity, extending

the dynamic range of your analysis.

The ACQUITY UPLC M-Class System can

be easily combined with all types of mass

spectrometers, whether analyzing complex

samples in wide-ranging matrices with

a high resolution MS, or quantifying

peptides, metabolites, or other targeted

experiments with tandem MS.

Separation of small molecule standards on 1.0 mm I.D. (left) and 300 µm I.D. (right) columns with the ACQUITY TUV Detector, 100 nL flow cell. The 1.0 mm and the 300 µm I.D. columns provide comparable chromatographic resolution with a scaled sample load.

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8.0e-2

1.0e-1

1.2e-1

1.4e-1

1.6e-1

1.8e-1

2.0e-1

2.2e-1

2.4e-1

2.6e-1

2.8e-1

3.0e-1

3.2e-1

3.4e-1

Gradient slope: 10% per CV5-95%B linear A= water/0.1% formic acidB= acetonitrile/0.1% formic acidλ 254ACQUITY UPLC M-Class BEH C18, 1.7 µm, 1.0 mm x 150 mm Column

4.0 ng of each injected:2-AcetylfuranAcetanilideAcetophenonePropylphenoneButyl ParabenBenzophenoneValerophenone

Time-0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00

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0.0

2.0e-2

4.0e-2

6.0e-2

8.0e-2

1.0e-1

1.2e-1

1.4e-1

1.6e-1

1.8e-1

2.0e-1

2.2e-1

Gradient slope: 10% per CV5-95%B linear A= water/0.1% formic acidB= acetonitrile/0.1% formic acid λ 254ACQUITY UPLC M-Class BEH C18, 1.7 µm, 300 µm x 150 mm Column

0.4 ng of each injected:2-Acetylfuran AcetanilideAcetophenonePropylphenoneButyl ParabenBenzophenoneValerophenone


Because of its advanced fluidic pathways,

the ACQUITY UPLC M-Class System

delivers highly concentrated analyte

to the MS.

Maximize recovery

The non-reactive flowpath, unique

to the ACQUITY UPLC M-Class System,

ensures recovery of biologically

important molecules.


Sample integrity.

Changes in phosphorylation can correlate to physiological changes and disease states. The recovery of multiple phosphorylated peptides, demonstrated here, addresses concerns of researchers who fear missed data could lead to poor quality decisions.

Sample: The MassPREP™ Enolase Digestion (p/n 186002325) with four added yeast enolase phosphopeptides (three are singly phosphorylated and one is doubly phosphorylated) – T18p, T19p, T43p, T43pp. The system design is verified with the appropriate standard to demonstrate successful resolution.Time

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100 13.25

14.40

18.1316.32 18.82

18.24

17.9215.32

20.09 35.3122.6521.42 24.9123.33 25.91 31.3428.3626.61 30.51 31.9932.90 37.26

19.17

18.79

18.2414.6313.2511.57 17.0616.30

21.4320.09

19.0824.86

22.6537.95

37.7625.38 36.9435.2733.9138.94

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T19 1P

T18 1P

T43 2P

T43 1P

COMP REHENSIV E 2D – EX PANDING T HE IMPACT

T HE FLEXIBLE DESIGN OF THE ACQUIT Y UPLC M-CLASS SYST EM OFFERS:

Until recently, 2D-LC was considered

both time-consuming and difficult to

accomplish. The ACQUITY UPLC M-Class

System has streamlined the 2D-LC

separation process with highly intuitive

menu-driven method setup, standardized

separation chemistries, and intelligent

valve operation.

Expanding on the use of a novel 2D

reversed phase/reversed phase (RP/RP)

approach, where the 1st dimension is

run at pH 10 and the 2nd at pH 2,

grants the ability to robustly increase

sample throughput.

The 2D configuration extends the dynamic

range of the analysis and yields itself to

host cell protein (HCP) analysis – removing

the need for cumbersome ELISA analysis.

0.0E+01.6E+6

0.0E+08.0E+5

0.0E+09.0E+5

0.0E+0

0.0E+0

5.0E+5

4.0E+5

Inte

nsity

0 5 10 15 20 25 30 min

Fraction 1

Fraction 2

Fraction 3

Fraction 4

Fraction 5

Separation of a MassPREP Digest Standard Mix 1 using a five step elution profile.

■■ Smaller I.D. columns for

increased sensitivity with

minimal sample use

■■ Optical detection with

ACQUITY® TUV and

PDA Detectors

■■ Optional non-reactive

flow paths

■■ Microscale electrospray

(ESI) probe for a ZSpray

API interface

■■ Comprehensive 2D analysis

■■ HDX capability

The ACQUITY UPLC M-Class System for 2D analysis.

[ ACQUITY UPLC M-CLASS SYSTEM ][ ACQUITY UPLC M-CLASS SYSTEM ]

With the increase in data quantity and quality along with improvements in DynamX HDX Data Analysis Software, researchers are in the best position to unlock the meaning of their data and improve decisions.

Butterfly plot comparing the deuterium uptake between two different states: bound (bottom) vs. unbound (top).

Expanding on the initial collaboration with

thought leaders worldwide, Waters has

extended the HDX capabilities of the

ACQUITY UPLC M-Class System. The higher

pressure envelope enables even faster

chromatographic resolution of peptic peptides.

The system integrates all the steps of

HDX from sample prep to actual sequence

determination, deuterium uptake curves, and

heat maps with the innovative DynamX™ HDX

Data Analysis Software.

Maximum chromatographic resolution


enables columns packed with sub-2-µm

particles to improve nano- to microscale

separations. The Enzymate™ BEH Pepsin

Column adds a new level of control to

the entire HDX experiment.

MONITOR P ROT EIN CONFORMATION WIT H HDX T ECHNOLOGY

Be Assured. Choose Waters Global Services.

Waters Global Services helps

customers optimize laboratory

operations by providing superior

service, support, upgrades,

training, and Waters Quality Parts.®

For more information, go to

www.waters.com/services.

The ACQUITY UPLC M-Class System is designed for any nano- to

microscale separation you need to run. In addition Waters offers 75 µm

fused silica capillary columns, 150 µm iKeys, 300 µm stainless steel

columns, and access to traditional 1.0 mm I.D. columns. Depending on

your laboratory needs, we have an option for you.

ACQUITY UPLC M-CLASS COLUMNS

ACQUITY UPLC M-Class Columns are specifically

engineered for use with the ACQUITY UPLC M-Class

System and are designed for the high peak capacity

required for sample-limited applications. With 10 years

of UPLC sub-2-µm column design, these new columns

continue a tradition of reliability and performance.

of nano- to microscale chemistries

full range

iK EY SEPARAT ION DEV IC E

Ongoing collaboration with customers

uncovered a need for simplified microscale analysis.

The iKey™ Separation Device, with its ionKey™ Source,

is specifically designed to address this need, providing high

sensitivity separations to users doing routine analysis.

Its 150 μm I.D. provides optimum sensitivity and instrument

duty cycle.


The ACQUITY UPLC M-Class System and SYNAPT G2-Si delivers high peak capacity separations with exceptional LC/MS data quality.

Time (min)

Peak capacity = 578 1 µg of a tryptic-digested50 kDa protein

75 µm x 150 mm CSH130 C18, 1.7 µm

55 min


expands the performance of any nano-

to microscale MS analysis. It increases

the linear dynamic range of your analysis,

resulting in better MS analyses by

DDA or MS.E

The direct (non split) automatic solvent flow

control algorithms of the ACQUITY UPLC

M-Class System provide pulse-less flow for

increased retention time reproducibility.

Sample integrity is maintained due to

the ACQUITY UPLC M-Class System’s

non-reactive surfaces. New drivers for

MassLynx Software integrate the system

seamlessly into your existing workflows.

yourinlet to MS

Intuitive system-level diagnostics

Enables interactive display of system fluidic status to ensure system integrity and maximum performance – especially when changing columns, tubings, and fittings.

ACQUITY UPLC M-Class System with SYNAPT® G2-Si.

SALES OFFICES:

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Brazil 55 11 4134 3788

Canada 1 800 252 4752

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Czech Republic 420 2 617 11384

Denmark 45 46 59 8080

Finland 358 9 5659 6288

France 33 1 30 48 72 00

Germany 49 6196 400 600

Hong Kong 852 2964 1800

Hungary 36 1 350 5086

India 080 492292200 03

Ireland 353 1 448 1500

Israel 9723 3731391

Italy 39 02 265 0983

Japan 81 3 3471 7191

Korea 82 2 6300 4800

Mexico 52 55 52 00 1860

The Netherlands 31 76 508 7200

Norway 47 6 384 6050

Poland 48 22 101 5900

Portugal 351 21 893 61 77

Puerto Rico 1 787 747 8445

Russia/CIS 7 495 727 4490 / 290 9737

Singapore 65 6593 7100

Spain 34 93 600 9300

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Switzerland 41 56 676 7000

Taiwan 886 2 2501 9928

UK 44 208 238 6100

US 1 800 252 4752

Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 508 478 2000 F: 508 872 1990 www.waters.com

www.waters.com/mclass

www.waters.com/mclasscolumns

Waters, The Science of What’s Possible, ACQUITY, ACQUITY UPLC, UPLC, MassLynx, Symmetry, SYNAPT, and Waters Quality Parts are registered trademarks of Waters Corporation. DynamX, Enzymate, ionKey, iKey, MassPREP, and ZSpray are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2014 Waters Corporation. Printed in the U.S.A.March 2014 720004841EN LM-SIG

http://www.waters.com/mclass

http://www.waters.com/mclass

let your lc inlet do the work - waters … your lc inlet do the work for your nano- to microscale...

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