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LabRAM Series

Whether the goal is qualitative or quantitative data, Raman spectroscopy can provide key information, easily and quickly,detailing the chemical composition and the structure of theinvestigated material.

Pharmaceuticals and Cosmetics

Raman chemical image of pharmaceutical tablet showingdistribution of the three major constituents.

Semiconductors

Confocality and spatial resolution is pushed to the sub-micron limit.This example shows 250 nm features of a semiconductor structureresolved using our confocal micro Raman system.(Data courtesy of ATMEL Rousset Université Paul Cezanne, France)

Polymers

A chemical image of four micro-spheres using the LabRAM ARAMISRaman microscope showing, the high spatial resolution possiblewith this technique.

Geology / Mineralogy / Gemmology

High resolution Raman mapped image of a mineral sample, showing Metamictisation of ZrSiO4 by self irradiation due toradioactive decay of Uranium and Thorium incorporated in zircon crystals. (Data courtesy of Dr. L. Nasdala, University of Mainz,Germany)

Life Science

3D image of single cell bacteria on a substrate. The high spatialresolution enables single cells to be characterized and studied ingreat detail. (Data courtesy of Dr Wei Huang, CEH Oxford, UK)

Applications Art • Biomedical • Carbon • Catalysis

Chemistry • Forensics • Geology • MaterialsPharmaceuticals • Physics • Polymers

Process • Semiconductors

The advantages of Ramanspectroscopy

• Non destructive• Non-invasive• No need for sample preparation• Information rich• Highly specific chemical fingerprints

Fields of Application

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World leaders in RamanspectroscopyThe HORIBA Jobin Yvon Raman Division is the world leader inRaman spectroscopy. We have designed and manufactured state-of-the-art Raman systems for over four decades, and introducedground breaking innovations such as the first commercial Ramanmicroscope, the first holographic diffraction gratings and theworld’s first remote Raman sampling probe.

The Raman Division consistsof the combined expertise ofthe Jobin Yvon, Dilor andSPEX brand names and isproud of having more than5000 Raman systems installedworldwide.

Our Raman instruments are available as part of a complete rangeof HORIBA analytical, research and industrial solutions. TheHORIBA group has become one of the world's largestinstrumentation companies and has a reputation for supplyingreliable and high performance equipment in the environmental,research, analytical and process monitoring areas of application.

Looking for Raman: Why look any further?

Performance throughinnovative technologyThe Raman microscope has become an invaluable analytical toolfor many different applications including pharmaceuticals,polymers, semiconductors, forensics and even life science.

The latest series of LabRAM Raman microscopes incorporatesleading edge technology in detectors, lasers and optics to providefast, sensitive and flexible analytical measurements. The LabRAMseries features the true confocal Raman microscope for the highestpossible spatial and depth resolution. Analysis of samples down tothe micron scale and below, and automated high definition Ramanimages can be rapidly and easily obtained.

LabRAM Series of Raman MicroprobesFeature

Automated Raman mapping and imagingTrue confocal Raman microscope Versatile micro and macro sampling Multiple laser excitations from UV to NIRCombination with FTIR, PL, TCSPC and AFM technologiesRemote in-situ samping capabilities

Fast Raman Images

The advanced optical technology, high sensitivity and powerfulsoftware of the LabRAM systems provide access to faster andmore detailed chemical images. The LabRAM systems canmaximize your laboratory output.

Raman mapped pixelated image showing cluster chemometricanalysis of cells. (Data courtesy of M. MANFAIT, Univ de Reims,France)

Raman Spectroscopy

Spectral resolution and rangeThe LabRAM system incorporates a fast achromatic Czerny-Turner spectrometer with a rugged high-stability design. Ramananalysis with unsurpassed spectral resolution and range gives themost detailed chemical analyses and images.

The high resolution version, the LabRAM HR, is the most powerfulanalytical system available on the market with exceptional spectralperformance. Even in the UV, its unique 800 mm focal lengthspectrograph provides uncompromised efficiency and spectralresolution. No other Raman system offers such easily accessedperformance.

High throughput optics optimized for the UV, VIS, NIR rangeHigh precision spectrometer for reliable and robust Raman spectraMotorized computer controlled operation for ease of use and speed of acquisition.High precision grating turret offers easy optimisation for different lasers or resolutionsTrue confocal microscope for the most detailed images

CCl4 spectrum at room temperature obtained with a short focallength (300mm) and a long focal length (800mm) spectrograph. Clearseparation of the peaks is obtained only with the long focal lengthspectrograph.

Multiple laser excitationsThe Raman microsope benefits greatly from the use of differrentlaser wavelengths to expand the range of samples that may beanalysed. The LabRAM systems incorporate a high stabilityoptical design to enable fast and reliable switching for multiple lasersources. An internal HeNe (633nm) laser can be complemented byAr+, solid state, and diode lasers covering a broad range ofwavelengths, be it for resonance Raman optimized for sensitivity,for fluorescence suppression, or for sample penetration. Otherlaser sources can even be supplied for specialized UV and NIRapplications.

Patented 4 Rayleigh filter selector enables easy selection andoptimisation for different laser wavelengths

High stability rugged spectrograph designLong focal length for high spectral resolutionHigh precision grating turret (up to 4 gratings)Fiber port for remote probe measurementsDual detection ports for additional PL and NIR Raman measurements

Integrated true confocal microscopeStandard, Open or Inverted microscope Motorized adjustable confocal apertureAutomated wavelength selection (up to 4 lasers)Class I laser enclosure

Special features and accessoriesFTIR module: Reflective and contact ATR microscopyMacro chamber: 90° and 180° excitation Fluorescence microscopy and extended visualisation optionsHeating/cooling stages

System Concept

True confocal microscopeopticsThe true confocal entrance optics of the LabRAM microscopesoffer the ultimate performance in spatial resolution and depthdiscrimination. The most detailed Raman images are obtained withincreased speed and efficiency. The integrated design ensurestotal stability, whilst the continuously adjustable and motorizedconfocal aperture perfectly matches the investigated volume orparticle area.

Confocal microscope principle

Only true confocal performance provides the ultimate in Ramanmapped images and depth profiling. HORIBA Jobin Yvon confocalRaman microscopes approach the diffraction limit and offer thesmallest possible sampling volumes. Where correctly matchedobjectives and laser wavelengths are used the LabRAM systemcan reach sub-micron resolutions.

Confocal depth measurement on a laminar polymer film enables thecharacterisation of successive layers without cross-sectioning orsignificant blurring of layer boundaries

Macro measurementsThe macro chamber available on several of the LabRAMinstruments is designed to perform efficient Raman studies on bulk,solid or liquid samples. The chamber integrates on the side of theinstrument through direct coupling and enables both 90° and 180°scattering measurements for added flexibility.

Multipass effect for higher sensitivityDirect coupling for maximum efficiencyCapillaries and cuvette holdersXYZ and rotation sample holderViewing camera

Macro chamber withmultiple sample holders andcamera for viewing the sampleprior to analysis

Optimal laser rejectionThe LabRAM series offers state-of-the-art Rayleigh rejection filtersin order to ensure optimal laser rejection, increased throughputefficiency and extented filter lifetime. Standard holographic filtersare also available for where special antistokes Ramanmeasurements are required.

The low frequency region of the Raman spectrum down to30 cm-1 or less is reached through the use of our low frequencyfilter options. This information-rich region is ideal for the analysisof lattice vibration bands such as those found with subtle drugpolymorphism and crystal phases, with many inorganic samplesand with carbon nanotubes.

Low frequency Raman spectrum of sulphur illustrates the easyaccess to sub 100cm-1 ranges with the LabRAM series

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Air

Polymer layer 1

Polymer layer 2

Polymer layer 3

25µm

Robust and sensitive Raman The LabRAM family from HORIBA Jobin Yvon offers a completerange of compact, dedicated high performance Ramanmicroscopes suited to both the scientific researcher and theindustrial analyst.

This new generation of technically advanced instrumentationcombines ease of operation with superior performance. TheLabRAM provides enhanced sensitivity, flexibility and long termstability. Incorporating new and optimized high throughput singlespectrographs, measurement times are extremely fast, requiringoften only seconds to obtain a complete and reliable Raman"fingerprint".

Raman images can be recorded quickly and with a full spectrumcontained in each pixel. The speed of measurement and softwarecapabilities allow each spectrum to be obtained in milliseconds (foran example see the image below right). Switching between modesof operation, laser sources and resolution options is quick with fullyautomated versions available to maximize laboratory throughput.

Versatile sampling andtechnologyThe LabRAM systems are ideally suited to micro and macromeasurements, to 2-D and 3-D imaging and depth profiling. TheTRUE confocal microscope enables the most detailed images andanalyses to be obtained with speed and confidence.

Highly versatile, each LabRAM can be supplied as a base unitwhich can be expanded with a range of options, upgrades andaccessories to suit all budgets and applications. Specialized,dedicated or customised solutions can even be supplied whererequired, so, whatever spectral resolution, laser wavelength orsampling regime is needed HORIBA Jobin Yvon can provide asolution.

Be it for analytical, industrial or research measurements there is aLabRAM system ideally matched for you.

YOUR CHOICE !

The LabRAM series designed for performance,engineered for reliability.

LabRAM ARAMIS

High Performance Raman Made Easy

The LabRAM ARAMIS offers automated, intelligent Ramananalysis with uniquely simple but high performance operation.

Patented QUAD grating and filter technology ensures fast andsensitive Raman imaging. Automation and reliability providessimple and effective “point and shoot” chemical analysis.

Spectra of Ibuprofen taken at three different resolutions. TheLabRAM ARAMIS easily illustrates the subtle Raman information thatcan be found when the higher spectral resolution mode is used(green spectrum shown above)

Fast Raman map of pharmaceutical crystals with 100ms/point

LabRAM Series

LabRAM HR

High Resolution Raman

The integrated and robust high performance Raman microscope.The LabRAM HR features high spectral resolution, multiple laserand detector options, UV-VIS-NIR range options, and completeflexibility. It offers the highest performance Raman microscope onthe market and is ideally suited to pushing research to the verylimits.

LabRAM HTS

The ideal system for High Throughput Screening

The LabRAM HTS version of the family is optimized for rapid androutine Raman analysis and assays. Specialized automaticwellplate handling and on-line data analysis and interrogationprovide a powerful tool for pharmaceutical and chemical HTSrequirements.

LabRAM IR²

Combined Raman and FTIR on the same system

The unrivaled dual Raman and FTIR LabRAM system thatuniquely couples the confocal Raman microscope withcomplementary FTIR micro analysis.

For the first time a single bench-top system offers the completemolecular vibrational characterisation from the same samplelocation in a fast and robust single package.

LabRAM INV

The best for Bio-Chemical studies

The LabRAM INV is an innovative inverted Raman microscopewith either standard or high resolution versions. It provides trueconfocal microscope performance and full inverted Ramanmicroscopy. Ideally suited to life science and biological samplessuch as cell and tissue media, the LabRAM INV is the perfectanalytical tool for the bio and life science researcher.

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Raman and FTIRThe unique LabRAM IR² system combines both micro Raman andmicro FTIR analysis upon the same instrument enablingcomplementary spectroscopies to be undertaken easily. Thisheralds the beginning of hybrid Raman analysis, where severalspectroscopies can be combined to provide a wider analyticalpicture.

Combined Raman and FTIR in-situ measurements of a Pd/Al2O3catalyst. Absorbed species are best analysed using FTIR while lowerfrequency oxide components are characterised using Raman. (Datain collaboration with Dr E Payen LCL, Villeneuve d’Ascq, France)

Raman and AFMThe use of Raman microscopy has become an important tool forthe analysis of materials on the micron scale. The unique confocaland spatial resolution of the LabRAM series has enabled optical farfield resolution to be pushed to its limits with sub-micron resolutionnow achievable. To develop spatial resolution still further and intothe near field, the LabRAM series provides an ideal platform forcombining Raman with AFM and scanning probe microscopy.Specialised interfaces allow new and emerging techniques such asTERS and SNOM, to be explored with confidence.

250 nm pillars of strained Si onSiGe substrate. AFM image(a). Structure induced stressmonitored using Si Ramanpeak shift (b)

Raman andPhotoluminescenceHORIBA Jobin Yvon offers unique Raman/PL dedicatedconfigurations for the LabRAM HR and LabRAM ARAMIS. Using acombination of both vibrational and electronic/optical analyses thecombined Raman/PL system yields a greater depth of informationon many important materials.

This is particularly true in the characterization of semiconductormaterials, quantum dots and carbon nanotubes and can provideinformation directly related to sample structure, the productionconditions, homogeneity, purity and performance.

PL excited at 532nm and detectedin NIR range of three differentsemiconductor materials usingan InGaAs array detector

The screenshot shows Ramanspectra and PL maps of asilicon wafer, derived fromthermal donor characteristics

Raman and TCSPCTime-Correlated Single Photon Counting (TCSPC) is afluorescence lifetime measurement technique which providessensitive probing of low light level signals into the picosecondrealm. This technique can now be coupled to the LabRAMinstruments. Both vibrational and fluorescence lifetime informationcan be recorded from the same sample on the same equipmentplatform.

This unique innovation opens the door to new applications andnew solutions in the fields of analytical chemistry, biomedicalimaging and material science.

Fluorescence lifetimeimage obtained on a TCSPCmicroscope attachmentfrom HORIBA Jobin Yvon

Advanced Techniques

FTIR Raman

After cooling

NO exposure

After heating &reduction

Bulk Pd/Al2O3

a

b

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Raman imagingThe new generation of Raman confocal microscopes offers anon-destructive method of sample analysis at the micron andsub-micron level. It possible to obtain fast and accurate ‘chemical’images. Raman mapping from HORIBA Jobin Yvon has beendesigned to produce the fastest and most detailed Raman images.It is achieved by moving the sample with a precise motorized XYsample stage or by scanning the laser in the sample plane. Acomplete and confocal Raman spectrum is obtained for each pointon the sample. The Raman hyperspectral image is thenreconstructured in the sophisticated LabSpec Raman software toproduce the highly accurate final image.

Three-dimensional imaging of heterogeneous samplesExtended video mode to visualize the sample beyond the microscope field of viewChemical component distribution with sub-micron resolutionFast mapping capability

Images of isolated Single-Wall Carbon Nanotubes (SWCNTs) ofdifferent diameters on a Silicon wafer are generated from specificspectral features

Patented LineScan system for the fastest Raman imaging

Fast mappingAdvances in detection technology and optical design combine withnew software capabilities to speed up the acquisition of Raman‘chemical’ images. At the forefront of microscopy innovations,HORIBA Jobin Yvon has developed pioneering solutions forRaman imaging and offers ground-breaking acquisition times.

Raman map generated with 200ms/point and 1µm step, providingchemical identification of the various crystals

Mapping accessoriesWhere applications demand, specialized microscope options andaccessories can be readily interfaced to our Raman systems.

High precision motorized XY stages (up to 300 x 300mm)Z axis translation for depth profilingFast and accurate autofocusPiezo driven stages for ultimate precision in X, Y and Z

High resolution 200 x 200 x 200 µm piezo electric stage

Hyperspectral Imaging

157.8 - 161.7 cm-1

262.9 - 270.6 cm-1 165.6 - 169.5 cm-1

LabSpec softwareThe LabSpec software has been developed by our in-house teamof software engineers. It has been designed for Raman,luminescence and hyperspectral imaging measurements. Thishighly powerful software offers an efficient and easy-to-use tool forboth research and analytical operators. Icon driven screens andwizards control the different instrument functions, data acquisitionand data processing.

Raman image generation The powerful Raman imaging functions of LabSpec enable Ramanmaps to be produced from a range of parameters including bandposition, integral, FWHM, correlation fit to models, etc.

Active ingredients identification and distribution in a pharmaceuticaltablet

Full function controlAutomatic ND filter wheel, confocal aperture and excitation wavelength selection Motorized stages for XY and Z mapping and autofocusVideo image capture and digital storageAutocalibration routinesExternal triggering and additional detection systemsPowerful Visual Basic Script (VBS) editor for customizedautomated routines and experiments

Extensive data treatment Band fitting and de-convolutionSpectral subtraction and baseline correctionUnivariate and multivariate analysisLinear and nonlinear filtering functionsData file export/import and compatibility with commercialspectral search softwares (e.g. direct data links with Spectral IDlibrary module)

Spectral libraries LabSpec enables fast and user-friendly access to spectral librariesthrough the Spectral ID module. The active Raman spectrum canbe automatically searched from within LabSpec to enable simplechemical identification of an unknown compound. Powerfulalogorithms find the best match and assign a quality value to theassignment of the sample.

Compatible with most commercially available databasesCreate and manage new customized libraries

The Spectral ID module is provided with the HORIBA Jobin Yvonlibraries containing over 1500 spectra of organic and inorganiccompounds, pharmaceutical ingredients, semiconductors,polymers, minerals, ancient art pigments, etc.

Advanced chemometricanalysisISysTM has become the industry standard imaging software foradvanced chemometric analysis. The software offers robustassessment of chemical image data by providing flexible filemanagement (Import and Export), data preprocessing, includingspectral and spatial preprocessing, powerful data visualizationtools, and a range of alternative methods of Statistical PatternRecognition.

Software

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Microscope options

Where applications demand, specialized microscope options andaccessories can be readily interfaced to our Raman systems.

Unique Free Space Microscope(FSM) for larger samples and sam-ple cells

Multiwell plate for automatedhigh throughput screening

Optimized long working distance,UV and NIR microscope objectivesfor optimal results

Dual microscope options forextented versatility

Many specialized illumination and visualization options can beadded to our Raman microscope systems in order to enhancecontrast and sample observation.

Binoculars/TrinocularsPolarized light visualizationDark field illuminationDifferential Interference Contrast Phase contrastFluorescence microscopy

Remote sampling accessories The LabRAM microscopes can couple to a range of samplingaccessories, which enable in-situ, non-invasive chemical analysesto be undertaken. Typically connected via optical fibres, a ruggedRaman sampling probe is ideal for remote Raman measurementsand is well adapted to process monitoring and control applications.

SuperHead high efficiency fiber probeVideo probe with white light imagingConfocal probe for high spatial resolution and depth profilingVarious macro sampling options and accessoriesWide selection of resistant immersion accessories

Specialized stagesA range of specialized stages can be attached to the instrument forin-situ analysis in various conditions:

Heating/Cooling stages up to 1500°C or down to 4KCatalytic Operando cell reactor for corrosive or conductive carrier gases Controlled humidity (vapor pressure) stagesElectrochemical cellsDiamond anvil and pressure cells

Heating / cooling stage and catalytic cell are readily interfaced under the LabRAM Raman microscope

Detection optionsThe LabRAM systems have a dual detector port capability andoffer a large range of detector options to enable the broadest rangeof possible measurements. CCD detectors, NIR detectors, and fastsingle channel detection are all possible upon the same instrument.

Backthinned, open electrode and front illuminated CCD chips canoffer enhanced detection. All are provided as a high grade 1024 pixel1" CCD chip format.

Peltier or liquid nitrogen (LN2) coolingUV, visible or NIR enhanced CCD chipsLarge high resolution 2048 x 512 pixel CCD chip optionIR array (InGaAs) detection for measurement up to 1.6 µmiCCD for time-resolved measurementsSpecialized single channel IR detectors for measurements up to 2.5 µm or for time resolved applications

Options - Accessories

OPTICALSPECTROSCOPY

DETECTORS

OEM SPECTROMETERSAND GRATINGS

CUSTOM GRATINGSAND VUV BEAMLINES

ELLIPSOMETRY

THIN FILMPROCESS CONTROL

• The content of this catalogue is subjectto change without prior notice

• It is forbidden to copy from the contentsof this catalogue in part or in full

• Please read the instruction/operation manualbefore using these products

This instrument complies with 21CFR 1040.10 and IEC 60825-1 (08/2001)

FLUORESCENCE

ATOMIC EMISSION SPECTROSCOPY/

ELEMENTAL ANALYSIS

RAMANANALYSIS

X-RAY FLUORESCENCE

PARTICLE SIZE ANALYSERS

FORENSICS

www.jobinyvon.com

France : HORIBA Jobin Yvon S.A.S., 231 rue de Lille, 59650 Villeneuve d’Ascq. Tel : +33 (0)3 20 59 18 00Fax : +33 (0)3 20 59 18 08 - Email : raman@jobinyvon.fr - www.jobinyvon.fr

USA : HORIBA Jobin Yvon Inc., 3880 Park Avenue, Edison, NJ 08820-3012. Toll-free : +1-866-jobinyvonTel :+1-732-494-8660 - Fax : +1-732-549-2571 - Email : raman@jobinyvon.com - www.jobinyvon.com

Japan : HORIBA Ltd., JY Optical Sales Dept., 1-7-8 Higashi-kanda, Chiyoda-ku, Tokyo 101-0031.Tel : +81 (0)3 3861 8231 - Fax : +81 (0)3 3861 8259 - Email : raman@horiba.com

Germany : +49 (0) 6251 84 75-0 Italy : +39 02 57603050 UK : +44 (0)20 8204 8142China : +86 (0) 10 6849 2216 Other countries : +33 (0)1 64 54 13 00

Follow the Experts

λ = 325 – 1064 nm, P≤500 mWVISIBLE OR INVISIBLE LASER

RADIATIONAVOID EXPOSURE TO BEAMCLASS 3B LASER PRODUCT

COMPANY PROFILEHORIBA Jobin Yvon, is one of the world’s largest manufacturers of analyticaland spectroscopic systems and components and we are committed to servingour customers with superior products and technical support.

Established in 1819, Jobin Yvon is part of the HORIBA Group which employsmore than 4,000 people worldwide, with annual sales in excess of$935,000,000. HORIBA, Jobin Yvon, Sofie, Dilor, SPEX and IBH are someof our well known and respected brand names.

CLASS 1 LASER PRODUCT

*Laser safety classifications depend on individual systems and options