Dan Feldman

“Nanonaps” for Photothermal therapy on Balb/Cmice with 4T1 (cancer cell line) tumors

7/6/15

ONC

F127Fig 1

700 750 800 850 9000

0.05

0.1

0.15

0.2

0.25

0.3

0.35

Wavelength (nm)

Abso

rban

ce

860 wavelength, before fridge

700 750 800 850 9000

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

0.45

Wavelength (nm)

Abso

rban

ce

880 wavelength, after 24 hours in fridge

0 0.5 1 1.5 2 2.5 3 3.5 4 4.5862864866868870872874876878880882

Wavelength effected by DCM con-centration

DCM concentration (ml)

Wav

elen

gth

0 2 4 6 8 10 12 14 16857

859

861

863

865

867

Wavelength effected by F127 con-centration

F127 concentration (ml)

Wav

elen

gth

(nm

)

Pre Wash

Wash 1

Wash 2

Wash 3

0 20 40 60 80100

120140

160180

200

Series1

Size (nm)

Fig 2

Dye combinations

600 650 700 750 800 850 900 950 10000

0.1

0.2

0.3

0.4

0.5

0.6

250 ul ONC and 125 ul ZnBNc

Wavelength

Abso

rban

ce

600 650 700 750 800 850 900 9500

0.1

0.2

0.3

0.4

0.5

0.6

250 ul ONc 250 ul ZnBNc

Wavelength

Abso

rban

ce

600 650 700 750 800 850 900 950 10000

0.10.20.30.40.50.60.70.80.9

250 ul ONC 500 ul ZnBNc

wavelength

abso

rban

ce

600 650 700 750 800 850 900 950 10000

0.10.20.30.40.50.60.70.80.9

1

500 ul onc and 250 ul znbnc

wavelength

abso

rban

ce

ZnBNc (707 nm) experiences a red shift when combined with ONc (860 nm)

860 upconverting nanoparticles with Vaseline

0 50 100 150 200 250 30030

35

40

45

50

55

60

65

70

Temperature Vs. Time Of Different O.D.

0 O.D.12.5 O.D.25 O.D.50 O.D.

Time (Seconds)

Tem

pera

ture

(Cel

cius)

Power Density = 550

0 50 100 150 200 250 30030

35

40

45

50

55

60

65

70

Temperature of Tumor

50 ul NNP100 ul NNP200 ul NNP

Time (Seconds)

Tem

pera

ture

(Cel

cius)

Tumor, 200 ul (100 OD), 0 seconds

Tumor, 200 ul (100 OD), 30 seconds

Tumor, 200 ul (100 OD), 1.5 minutes

PD= 400

20 days after PTT

100 OD injection 50 OD injection

0 10 20 30 40 50 60 70 80 90 100 110 1200

0.02

0.04

0.06

0.08

0.1

0.12

Absorbance of nanonap after injection into tumor

Injected O.D.

Abso

rban

ce

0

0.5

1

1.5

2

2.5

3

3.5x 10

4100 OD

0

0.5

1

1.5

2

2.5

3

3.5x 10

450 OD

0

0.5

1

1.5

2

2.5

3

3.5x 10

4Control, 0 OD

PAT images 24 hours after nanonaps were injected into the tail vein of mice

0 20 40 60 80 100 1200

0.1

0.2

0.3

0.4

0.5

0.6

0.7

Mass of dye in tumor

Injected OD

Mas

s of O

Nc (

mg

dye/

kg

tum

or)

0 20 40 60 80 100 1200

20

40

60

80

100

120

Measured O.D. in tumor

Injected O.D.

Mea

sure

d O

.D. a

fter t

umor

hom

ogen

izatio

n

100 OD Injected into mouse

0.5

1

1.5

2

2.5

3

3.5x 10

4

0.5

1

1.5

2

2.5

3

3.5x 10

4

At 860 nm laser At 820 nm laser