lab 3 goals and objectives: exercise 7: ubiquity of bacteria: observe results exercise 9: aseptic...
TRANSCRIPT
![Page 1: Lab 3 Goals and Objectives: Exercise 7: Ubiquity of Bacteria: Observe results Exercise 9: Aseptic Technique: Check results Exercise 10: Pure Culture Technique](https://reader033.vdocuments.us/reader033/viewer/2022051621/5697bf8a1a28abf838c8abcc/html5/thumbnails/1.jpg)
Lab 3 Goals and Objectives:
Exercise 7: Ubiquity of Bacteria: Observe resultsExercise 9: Aseptic Technique: Check resultsExercise 10: Pure Culture Technique
Check results, repeat once from your plate if necessaryRed = S. marcescens / Small yellow = M. luteus / Cream = E. coli
Exercise 11: Smear PreparationEach person make smear slides: ***Less is More!!!!
Your broth E. coli (from Ex. 9), Your slant E. coli (from Ex. 9)Interesting colonies (2-5) from ubiquity home plates (Exercise 7)
Exercise 12: Simple StainingEach person stain heat-fixed smear slides from Exercise 11***All stain and rinse water into tray: nothing down sink!!!
Hint for microscopy:Best smears will be barely visible to the naked eye when complete. In
order to find the focal plane put a marker dot on the finished dry slide, same side as the bacteria, before going to the scope and use the dot to find your focus.
Label a slide box with your name on tape and store any slides you wish to save. Blot oil off for storage. No methanol on slides (removes sample)
!!!CLEAN YOUR SCOPE WELL!!!
![Page 2: Lab 3 Goals and Objectives: Exercise 7: Ubiquity of Bacteria: Observe results Exercise 9: Aseptic Technique: Check results Exercise 10: Pure Culture Technique](https://reader033.vdocuments.us/reader033/viewer/2022051621/5697bf8a1a28abf838c8abcc/html5/thumbnails/2.jpg)
Bacterial ShapesCoccusCocciDiplococciTetradStreptococciStaphylococci
BacillusBacilliCoccobacilliStreptobacilliFusiform
SpiralVibrioSpirillumSpirochaetes
Lab book pg 101Sup. packet pg 56
![Page 3: Lab 3 Goals and Objectives: Exercise 7: Ubiquity of Bacteria: Observe results Exercise 9: Aseptic Technique: Check results Exercise 10: Pure Culture Technique](https://reader033.vdocuments.us/reader033/viewer/2022051621/5697bf8a1a28abf838c8abcc/html5/thumbnails/3.jpg)
No big blobs!Target circle optional: spread out wide to make smearas thin as possible
Must be COMPLETELY
dry!
Water first always with solid media
Figure 11.1
![Page 4: Lab 3 Goals and Objectives: Exercise 7: Ubiquity of Bacteria: Observe results Exercise 9: Aseptic Technique: Check results Exercise 10: Pure Culture Technique](https://reader033.vdocuments.us/reader033/viewer/2022051621/5697bf8a1a28abf838c8abcc/html5/thumbnails/4.jpg)
paper towel
Figure 12.2
![Page 5: Lab 3 Goals and Objectives: Exercise 7: Ubiquity of Bacteria: Observe results Exercise 9: Aseptic Technique: Check results Exercise 10: Pure Culture Technique](https://reader033.vdocuments.us/reader033/viewer/2022051621/5697bf8a1a28abf838c8abcc/html5/thumbnails/5.jpg)
Lab 3 Goals and Objectives:
Exercise 7: Ubiquity of Bacteria: Observe resultsExercise 9: Aseptic Technique: Check resultsExercise 10: Pure Culture Technique
Check results, repeat once from your plate if necessaryRed = S. marcescens / Small yellow = M. luteus / Cream = E. coli
Exercise 11: Smear PreparationEach person make smear slides: ***Less is More!!!!
Your broth E. coli (from Ex. 9), Your slant E. coli (from Ex. 9)Interesting colonies (2-5) from ubiquity home plates (Exercise 7)
Exercise 12: Simple StainingEach person stain heat-fixed smear slides from Exercise 11***All stain and rinse water into tray: nothing down sink!!!
Hint for microscopy:Best smears will be barely visible to the naked eye when complete. In
order to find the focal plane put a marker dot on the finished dry slide, same side as the bacteria, before going to the scope and use the dot to find your focus.
Label a slide box with your name on tape and store any slides you wish to save. Blot oil off for storage. No methanol on slides (removes sample)
!!!CLEAN YOUR SCOPE WELL!!!