krio rus imminst
TRANSCRIPT
KRIORUS.R
U
BY
DA
NI L
A M
ED
VE
DE
V
HISTORY
1900s – glycerol properties discovered by Russian botanist Maximov
1913 – experiments by Russian physicist Porfiry Bahmetiev on anabiosis and hypobiosis on larvae
1929 – the main character in Mayakovsky play “Bedbug” is cryopreserve
1971 – Russian reanimatologist Vladimir Negovsky discusses “European cryonics corporation” with Anatol Dolinov (France)
2000s – we catch up
OCTOBER 27, 2005
Cryonics case report at our seminar
THE BASIC FACTS
Founded 2005, owned by 9 people
15 patients
$10K neuro / $30K full-body
>50% market share in Europe
A for-profit (easier to register and operate in Russia), but a non-profit in spirit
5+ compensated part-time employees, many volunteers
STAFF
Valerija Pride, General Director
Igor Artyukhov, Science Director
Danila Medvedev, Chairman
Eugenij Shumilov, Doctor/Technician
Alexey Sulaev, Embalmer/Perfusionist
Andrey Shvedko, Engineer/Philosopher
Victor Grebenshikov, Head of Technical Council
CASES
4 E
XA
MP
L E C
AS
ES
EXAMPLE CASE 1
Patient: 80+ year old relative of a transhumanist
Death is in the hospital during the weekend (not in Moscow, but in major city)
Delay in notification: 12 hours
We arrive ~20 hrs after death
The body is cooled passively, core temperature reaches +9C
Additional time spent on formalities (due to the weekend): ~15 hrs
EXAMPLE CASE 1
Permission from the head of the hospital is obtained to perform cryopreservation (before the death certificate, so the patient is in custody of the hospital)
Head of the pathology department and a nurse assist
Our specialist oversees the procedure, prepares the solutions
BPS-7 is used as carrier, ramped up glycerol as cryoprotector
Access to vasculature is established, washout and perfusion is done manually (up to 50% glycerol
EXAMPLE CASE 1
Cooling is done using water ice and dry ice vapour, nasal temperature monitored
Solutions are cooled to a few degrees C
During the operation the core temperature is about +2
The brain had to be removed per relatives’ request
Cooldown to -80C in a dry ice box with no temperature adjustment (brain submersed in glycerol)
The brain is visually inspected, there is no ice formation visible on the surface
EXAMPLE CASE 2
Patient: ~25 year old (in Moscow, several years ago)
Delay in reporting: 5 hours. Additional delay: 10 hours
In that time we had to find a place to perform perfusion, find a surgeon, assemble the team, get necessary supplies in place, move the patient from the hospital, get necessary permissions
Access and perfusion done by surgeon, assistance by our specialist. A perfusion specialist
EXAMPLE CASE 2
Whole body perfusion takes ~7 hours
Whole body is preserved in dry ice
No way to ascertain the quality of the perfusion (venous return not analyzed for glycerol concentration)
A perfusionist analyzing the recording heavily criticized the operation
Later cellular studies show good viability in cryopreserved fibroblasts, sperm, other cells. Neurons not studied
EXAMPLE CASE 3
A 70+ year old relative of a transhumanist
Death in Moscow hospital (intensive care)
Agreement with administration reached pre-mortem
Notification about death: <1 hour
Access to the morgue in 2-3 hours
Medication including heparin is injected by our specialist, indirect heart massage is performed
EXAMPLE CASE 3
The body is additionally cooled with water ice while in morgue
The body is taken out of the hospital before government death certificate is issued (only with a medical death certificate) with the help of a funeral director
Perfusion takes place in the morgue near KrioRus
An embalmer experienced in doing open-circuit embalming perfusion (including 5+ cryonics cases) performs the operation
EXAMPLE CASE 3
A roller-pump is used with open-circuit perfusion
The operation is monitored using video-conferencing by a US cryonicist (Mike Darwin) who provides additional advice
Two nurses and our specialist assist
Blood return samples are taken
Head is preserved
Cooling is done with manual temperature control and tracking (in an acetone bath)
EXAMPLE CASE 4
An old dog is cryopreserved
The operation takes place in a private laboratory
A surgeon is performing the operation
A closed-circuit perfusion is used (takes 12+ hours for initial setup)
The operation starts on a live anesthetized dog
There is some difficulty establishing access
EXAMPLE CASE 4
The dog dies during the operation from shock
Due to insufficient monitoring the perfusate reservoir runs empty, perfusion has to be stopped earlier
A head of the animal is preserved and transported at -10 to the storage facility
FACILI
TIES
FACILITIES
FACILITIES
INTERNATIONAL COOPERATION
Can accept neuro and full-body patients for storage
Can pick up the patients in Europe, Russia, CIS
no standby/perfusion capability in EU
Are always willing to share information with local groups (friendships with groups in Finland, South Africa, Australia, Hong Kong, etc.)
Will offer EUCRIO standby to our clients
INTERNATIONAL COOPERATION
QUESTIONS?
www . kriorus . ru
kriorus @ mail.ru
+7-911-KRIORUS
Moscow, Russia