kevin l. gunderson et al. genome res. 2004; 14: 870-877 assembly of a random array 96 well...
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Kevin L. Gunderson et al. Genome Res. 2004; 14: 870-877
Assembly of a random array
• 96 well microtiter plate- 1 fibers bundle per well
• 1 bundle ~50,000 glass optical fibers w/ silica bead attached
• 1,536 bead types exist: yields @ 30-fold redundancy per SNP
GoldenGate SNP Genotyping Assay
J-B Fan et al. Cold Spring Harbor Symposia on Quantitative Biology, LXVIII, 69-78, January 2004
Genotyping data from one bundle
J-B Fan et al. Cold Spring Harbor Symposia on Quantitative Biology, LXVIII, 69-78, January 2004
Kevin L. Gunderson et al. Genome Res. 2004; 14: 870-877
Decoding images from eight stages
• Stage-by-Stage
• Core-by-Core
Genotype Calling – More difficult SNP
Mean GenCalls Across SNPs