Journal of Cell Science

Journal of Cell Science

VOLUME 98 1991

Editors

GARY BORISY (USA Editor) A. V. GRIMSTONE HENRY HARRIS R. T. JOHNSON

Editorial Board

RUDI APPELS PETER BARLOW MICHAEL BENNETT

ADRIAN BIRD ANTHONY BRETSCHER KEITH BURRIDGE

PETER COOK MICHAEL DEXTER GRAHAM DUNN

WILLIAM EARNSHAW DAVID GARROD BENNY GEIGER

IAN GIBBONS DAVID GLOVER SIAMON GORDON

CHRIS GRAHAM BRIAN GUNNING R. COLIN HUGHES

GORDON KOCH DAVID LANE RON LASKEY

CLIVE LLOYD HERBERT MACGREGOR CHRIS MARSHALL

FREDERICK MAXFIELD KENNETH MILLER SHIGEKI MIZUNO

PETER NEWELL BRUCE NICKLAS POTU RAO

KEITH ROBERTS JOEL ROSENBAUM ULRICH SCHEER

NICHOLAS SEVERS ERIK SCHWEITZER ALAN TARTAKOFF

JEAN-PAUL THIERY JEAN THOMAS GRAHAM WARREN FIONA WATT

Published by

THE COMPANY OF BIOLOGISTS LIMITED CAMBRIDGE

Typeset, Printed and Published byThe Company of Biologists Limited,

Department of Zoology, University of Cambridge, Downing Street,Cambridge CB2 3EJ

© The Company of Biologists Limited 1991

Contents

Volume 98 1991

CommentaryVasiliev, J. M.Polarization of pseudopodial activities: cytoskeletalmechanisms 1

Sugrue, P., Avolio, J., Satir, P.and Holwill, M. E. J.Computer modelling of Tetrahymena axonemes atmacromolecular resolution: interpretation ofelectron micrographs 5

Burgess, S. A., Dover, S. D. and Woolley, D. M.Architecture of the outer dynein arm ATPase in anavian sperm flagellum, with further evidence for theB-link 17

Chapin, S. J. and Bulinski, J. C.Non-neuronal 210xl03Mr microtubule-associatedprotein (MAP4) contains a domain homologous tothe microtubule-binding domains of neuronal MAP2and tau

cultured mesothelial cells from rabbit pericardia!cavity 93

Joswig, G., Petzelt, C. and Werner, D.Murine cDNAs coding for the centrosomal antigencentrosomin A

27

37

Bartles, J. R., Rao, M. S., Zhang, L., Fayos, B. E.,Nehme, C. L. and Reddy, J. K.Expression and compartmentalization of integralplasma membrane proteins by hepatocytes and theirprogenitors in the rat pancreas 45

Stearns, M. E., Wang, M. and Sousa, O.Evidence that estramustine binds MAP-1A toinhibit type IV collagenase secretion 55

Zurzolo, C, Gentile, R., Mascia, A., Garbi, C,Polistina, C, Aloj. L., Awedimento, V. E.and Nitsch, L.The polarized epithelial phenotype is dominant inhybrids between polarized and unpolarized ratthyroid cell lines 65

Reynolds, A. J., Oliver, R. F.and Jahoda, C. A. B.Dermal cell populations show variable competencein epidermal cell support: stimulatory effects of hairpapilla cells 75

Coleman, L. and Harris, A.Immortalization of male genital duct epithelium: anassay system for the cystic fibrosis gene 85

Honda, A., Noguchi, N., Takehara, H.,Ohashi, Y., Asuwa, N. and Mori, Y.Cooperative enhancement of hyaluronic acidsynthesis by combined use of IGF-I and EGF, andinhibition by tyrosine kinase inhibitor genistein, in

Hernandez-Verdun, D., Robert-Nicoud, M.,Geraud, G. and Masson, C.Behaviour of nucleolar proteins in nuclei lackingribosomal genes. A study by confocal laser scanningmicroscopy 101

Wang, X. and Traub, P.Resinless section immunogold electron microscopy ofkaryo-cytoskeletal frameworks of eukaryotic cellscultured in vitro. Absence of a salt-stable nuclearmatrix from mouse plasmacytoma MPC-11 cells 109

Symington, A. L., Zimmerman, S., Stein, J.,Stein, G. and Zimmerman, A. M.Hydrostatic pressure influences histone mRNA 125

CommentariesKuwano, M., Seguchi, T. and Ono, M.Glycosylation mutations of serine/threonine-linkedoligosaccharides in low-density lipoprotein receptor:indispensable roles of O-glycosylation 131

Bennett, D. C.Colour genes, oncogenes and melanocytedifferentiation 135

Palmberg, L., Lindgren, J. A., Thyberg, J.and Claesson, H.-E.On the mechanism of induction of DNA synthesis incultured arterial smooth muscle cells byleukotrienes: possible role of prostaglandinendoperoxide synthase products and platelet-derivedgrowth factor 141

Cassimeris, L. and Salmon, E. D.Kinetochore microtubules shorten by loss ofsubunits at the kinetochores of prometaphasechromosomes

Larsen, P. M., Chen, T.-L. L. and Wolniak, S. M.Neomycin reversibly disrupts mitotic progression instamen hair cells of Tradescantia

151

159

Akashi, T. and Shibaoka, H.Involvement of transmembrane proteins in theassociation of cortical microtubules with the plasmamembrane in tobacco BY-2 cells 169

Shingyoji, C, Katada, J., Takahashi, K.and Gibbons, I. R.Rotating the plane of imposed vibration can rotatethe plane of flagellar beating in sea-urchin spermwithout twisting the axoneme 175

Takahashi, K., Shingyoji, C, Katada, J.,Eshel, D. and Gibbons, I. R.Polarity in spontaneous unwinding after priorrotation of the flagellar beat plane in sea-urchinspermatozoa 183

Holifield, B. F. and Jacobson, K.Mapping trajectories of Pgp-1 membrane proteinpatches on surfaces of motile fibroblasts reveals adistinct boundary separating capping on the lamellaand forward transport on the retracting tail 191

Larkin, J. M. and Palade, G. E.Transcytotic vesicular carriers for polymeric IgAreceptors accumulate in rat hepatocytes after bileduct ligation

Tatham, P. E. R. and Gomperts, B. D.Rat mast cells degranulate in response tomicroinjection of guanine nucleotide

205

217

Nicholson, L. J. and Watt, F. M.Decreased expression of fibronectin and theintegrin during terminal differentiation of humankeratinocytes 225

Vessey, K. B., Ludwiczak, R. L., Briot, A. S.and Underwood, E. M.abnormal chromatin (abc), a maternal-effect locus inDrosophila melanogaster 233

Lee, R. E. and Kugrens, P.Attachment strips: a new type of hemidesmosome-like structure in the protozoon Katablepharis ovalisSkuja 245

Cossette, L. J. and Vincent, M.Expression of a developmentally regulated cross-linking intermediate filament-associated protein(IFAPa-400) during the replacement of vimentin fordesmin in muscle cell differentiation 251

CommentariesMarkl, J.Cytokeratins in mesenchymal cells: impact onfunctional concepts of the diversity of intermediatefilament proteins 261

Thome, K. J. I. and Mazza, G.Eosinophilia, activated eosinophils and humanschistosomiasis 265

Meier, J., Campbell, K. H. S., Ford, C. C,Stick, R. and Hutchison, C. J.The role of lamin LEI in nuclear assembly and DNAreplication, in cell-free extracts of Xenopus eggs 271

Belgrader, P., Siegel, A. J. and Berezney, R.A comprehensive study on the isolation andcharacterization of the HeLa S3 nuclear matrix 281

Beven, A., Guan, Y., Peart, J., Cooper, C.and Shaw, P.Monoclonal antibodies to plant nuclear matrixreveal intermediate filament-related componentswithin the nucleus 293

Meikrantz, W., Smith, D. M., Sladicka, M. M.and Schlegel, R. A.Nuclear localization of an O-glycosylated proteinphosphotyrosine phosphatase from human cells 303

Dilworth, S. M.A perichromosomal region contains proteinsphosphorylated during mitosis in Xenopus laeviscells 309

Shen, D.-W., Lu, Y.-G., Chin, K.V., Pastan, I.and Gottesman, M. M.Human hepatocellular carcinoma cell lines exhibitmultidrug resistance unrelated to MDR1 geneexpression 317

Woodcock, W., Williamson, I., Hassan, I.and Mackay, M.Isolation and characterisation of clones from theCaco-2 cell line displaying increased taurocholic acidtransport 323

Tuan, R. S., Moore, C. J., Brittingham, J. W.,Kirwin, J. J., Akins, R. E. and Wong, M.In vitro study of placental trophoblast calciumuptake using JEG-3 human choriocarcinoma cells 333

Boitano, S. and Omoto, C. K.Membrane hyperpolarization activates trout spermwithout an increase in intracellular pH 343

Morel, N., Brochier, G., Synguelakis, M. and LeGal La Salle, G.Immunological identification of a new 14xlO3 Afrmembrane-bound protein in Torpedo electric organ 351

Edwards, J. G., Campbell, G., Grierson, A. W.and Kinn, S. R.Vanadate inhibits both intercellular adhesion andspreading on fibronectin of BHK21 cells andtransformed derivatives 363

Margolis, L. B. and Popov, S. V.Induction of cell processes by local force

Sanders, E. J.Embryonic cell invasiveness: an in vitro study ofchick gastrulation

369

Tint, I. S., Hollenbeck, P. J., Verkhovsky, A. B.,Surgucheva, I. G. and Bershadsky, A. D.Evidence that intermediate filament reorganizationis induced by ATP-dependent contraction of theactomyosin cortex in permeabilized fibroblasts 375

Adams, J. C, Furlong, R. A. and Watt, F. M.Production of scatter factor by ndk, a strain ofepithelial cells, and inhibition of scatter factoractivity by suramin 385

Coopman, P. J., Bracke, M. E., Lissitzky, J. C,de Bruyne, G. K., van Roy, F. M., Foidart, J. M.and Mareel, M. M.Influence of basement membrane molecules ondirectional migration of human breast cell lines invitro 395

403

EMaz-Nido, J., Annas-Portela, R. and Avila, J.Addition of protease inhibitors to the culturemedium of neuroblastoma cells induces both neuriteoutgrowth and phosphorylation of microtubule-associated protein MAP-IB 409

Contents

Forer, A.In ultraviolet microbeam irradiations,characteristics of the monochromator and lampaffect the spectral composition of the ultravioletlight and probably the biological results 415

Holy, J. and Schatten, G.Differential behavior of centrosomes in unequallydividing blastomeres during fourth cleavage of seaurchin embryos 423

CommentariesBaserga, R.Growth regulation of the PCNA gene 433Jordan, E. G.Interpreting nucleolar structure: where are thetranscribing genes? 437

Earnshaw, W. C. and Cooke, C. A.Analysis of the distribution of the ENCENPsthroughout mitosis reveals the existence of apathway of structural changes in the chromosomesduring metaphase and early events in cleavagefurrow formation 443

Lamprecht, J., Schroeter, D. and Paweletz, N.Derangement of microtubule arrays in interphaseand mitotic PtK2 cells treated with deuterium oxide(heavy water) 463

Liu, B. and Palevitz, B. A.Kinetochore fiber formation in dividing generativecells of Tradescantia. Kinetochore reorientationassociated with the transition between lateralmicrotubule interactions and end-on kinetochorefibers 475

Liu, G. and Newell, P. C.Evidence that cyclic GMP regulates the associationof myosin II heavy chain with the cytoskeleton byinhibiting its phosphorylation 483

Nave, R., Fiirst, D., Vinkemeier, U.and Weber, K.Purification and physical properties of nematodemini-titins and their relation to twitchin

McCaig, C. D. and Dover, P. J.Factors influencing perpendicular elongation ofembryonic frog muscle cells in a small appliedelectric field

491

497

Bray, D., Money, N. P., Harold, F. M.and Bamburg, J. R.Responses of growth cones to changes in osmolalityof the surrounding medium 507

Duband, J.-L., Dufour, S., Yamada, S. S.,Yamada, K. M. and Thiery, J. P.Neural crest cell locomotion induced by antibodiesto pi integrins. A tool for studying the roles ofsubstratum molecular avidity and density inmigration 517

Farmer, A. A. and Gos8, S. J.BWTG3 hepatoma cells can acquire phenylalaninehydroxylase, cystathionine synthase and CPS-1without genetic manipulation, but activation of thesilent OTC gene requires cell fusion withhepatocytes 533

Oparka, K. J., Murphy, R., Derrick, P. M.,Prior, D. A. M. and Smith, J. A. C.Modification of the pressure-probe technique permitscontrolled intracellular microinjection of fluorescentprobes 539

Fritz, S. E., Hood, K. R. and Hood, E. E.Localization of soluble and insoluble fractions ofhydroxyproline-rich glycoproteins during maizekernel development 545

Knowles, G. C, McKeown, M., Sodek, J.and McCulloch, C. A. G.Mechanism of collagen phagocytosis by humangingival fibroblasts: importance of collagenstructure in cell recognition and internalization 551

Dargemont, C, Dunon, D., Salamero, J.,Deugnier, M.-A., Davoust, J. and Thiery, J. P.Overproduction and secretion of ^-microglobulin bya rat thymic epithelial cell line that expresses MHCclass I heavy chain 559

Armstrong, J. and Patel, S.The Golgi sorting domain of coronavirus El protein 567

Vandre', D. D., Centonze, V. E., Peloquin, J.,Tombes, R. M. and Borisy, G. G.Proteins of the mammalian mitotic spindle:phosphorylation/dephosphorylation of MAP-4during mitosis 577

Index of Authors 589

Index of Subjects 593

Contents

Information for contributors1 Manuscripts should be sent to The Editors, Journal of CellScience, Department of Zoology, Cambridge CB2 3EJ, England.Contributors in Canada and USA may send their manuscripts toDr Gary Borisy, Journal of Cell Science, Laboratory of MolecularBiology, University of Wisconsin, 1525 Linden Drive, Madison,Wisconsin 53706. (Bitnet number: BORISY® WISCMACC.)

2 Manuscripts should be in English and as concise as possible.They must be typewritten, in double spacing throughout (includ-ing tables, references and legends) on one side of the paper only.Each table should be typed on a separate sheet. Legends to figuresshould be typed in a single series and placed at the end of themanuscript. Three complete copies of the manuscript should besubmitted. Authors should retain a complete copy of the finalversion of their manuscript. Manuscripts must be fully ready forpublication; in the interests of rapid publication it will notnormally be possible to make alterations in proof other than thecorrection of typographical errors.

3 (a) Word-processor disks. Journal of Cell Science can useyour disk (51 or 31") instead of rekeying the accepted article.If available please send a copy of the disk with your final revisedmanuscript (disk will be returned).Please observe the following instructions1. Create and/or edit your manuscript, using the document mode

(or equivalent) in the word-processor program.2. Use only underline (or italic), bold, subscript and superscript.

Multiple font, style or ruler changes, or graphics inserted intothe text, reduce the usefulness of the disk.

3. Do not right-justify your text.4. Do not use footnotes.5. Use paragraph indents.6. Leave a blank line before and after all headings, which should

be underlined.7. Distinguish the numerals 0 and 1 from the letters 0 and 1.8. Also include an ASCII file on your disk (file without control

commands).Apple Macintosh disks. We can read standard 400k and 800kdisk formats. Please place the file in the root directory (do not usefolders) and save your file in MacWrite format if your wordprocessor allows it, or has a translator utility.BBC micro. If possible, send your file in ADFS format.

(b) Automatic text scanning. If you have no disk it may bepossible for us to 'scan' your manuscript (not photocopies) using aText scanning system':

Please ensure your manuscript is free from pen/pencil marks.Any alterations should be marked on a photocopy of yourmanuscript.

We cannot accurately scan manuscripts if the underline ruletouches the descending characters. If possible please provideanother manuscript without using this facility.

4 A short title of not more than 40 characters, should besupplied and at least 3 key words for indexing papers.

5 Manuscripts must contain a Summary of not more than 500words, placed immediately after the title page. This must beintelligible without reference to the main text and must containno references.

6 The list of References must be given in alphabetical order ofauthors' names. The titles of journals should be abbreviated inaccordance with the World List of Scientific Periodicals, 4th edn(1963) and Short Titles of Commonly Cited Scientific Journals,Royal Society, London (1980). The following style is used:Gray, E. G. and Willis, R. A. (1968). Problems of electron

stereoscopy of biological tissue. J. Cell Sci. 3, 309-326.Mazia, D. (1961). Mitosis and physiology of cell division. In The

Cell, vol. 3 (ed. J. Brachet and A. E. Mirsky), pp. 77-412. NewYork, London: Academic Press.

Citations in the text are given in the following form: Jones andSmith (1960) or (Jones and Smith, 1960). Where there are morethan two authors citations should be in the form (Jones et al.1960). Where more than one paper by the same author(s) has beenpublished in the same year they are cited as Jones (1960a), Jones(19606) etc.

7 Footnotes should be avoided wherever possible.

8 SI units should be used throughout in the preparation ofmanuscripts.

9 Illustrations: general. All figures, whether line drawings ormicrographs, should be numbered in a single series, in the orderin which they are referred to in the text. Each figure should benumbered separately (Fig. 1, Fig. 2, etc.); where a figure hasseveral parts use capital roman letters (Fig. 1A, IB, etc.). Themaximum printed size for illustrations is 235 mm x175 mm. In general, figures should not exceed in size about one-half of this. Whenever possible, plan illustrations so that, eithersingly or grouped together, they will be the width of a whole page(168 mm) or a single column (81 mm).

10 Line drawings. Original drawings, in black India ink,should be submitted for reproduction, as photographic copiescannot always be used. They should not be more than twicecolumn width.

11 Micrographs should be submitted the same size as they areto appear. Where several micrographs are grouped together theyshould be mounted accurately on mounting board. Irregularlyshaped micrographs should be avoided.

12 Labelling on figures may be inserted by authors provided itconforms to the general style and size used by the journal.Alternatively, it may be inserted by the printer, in which caselabelling should be indicated either on a duplicate, marked set offigures or on tracing paper overlays bearing accurately markedoutlines.

13 Colour plates can be reproduced at the discretion of theeditors and will be printed free of charge if they are judgedessential. Authors should note that, apart from being expensive,colour plates add significantly to the production time of thejournal.

14 Authors should retain a complete set of labelled figures forchecking against proofs. The originals will not be returned toauthors with proofs.

15 Authors will receive 200 offprints free of charge and mayorder additional copies when proofs are returned.

Copyright and reproduction1 In order to give The Company of Biologists Limited authorityto deal with matters of copyright, authors will be required toassign to them the copyright of any article published in thejournal.

2 Authors may make copies of their own papers in this journalwithout seeking permission from The Company of BiologistsLimited, provided that such copies are for free distribution only:they must not be sold.

3 Authors may re-use their own illustrations in other pub-lications appearing under their own name, without seekingpermission.

4 Specific permission will not be required for photocopyingcopyright material in the following circumstances:

(a) For private study, provided the copying is done by theperson requiring its use, or by an employee of the institution towhich he/she belongs, without charge beyond the actual cost ofcopying.

(b) For the production of multiple copies of such material, to beused for bona fide educational purposes, provided this is done by amember of the staff of the university, school or other comparableinstitution, for distribution without profit to student members ofthat institution, and provided the copy is made from the originaljournal.

5 For all other matters relating to the reproduction of copyrightmaterial written application must be made to Dr R. J. Skaer,Company Secretary, The Company of Biologists Limited, Depart-ment of Zoology, Downing Street, Cambridge CB2 3EJ, UK.

© The Company of Biologists Limited 1991

UNESCOInternational Cell Research Organization (ICRO)

EUROPEAN COMMUNITY

CELL CYCLE ANALYSIS AND

REGULATION OF EARLY EMBRYOGENESIS

A Practical Course Using Cells of Marine Organisms m

International Laboratory of Marine Cell Biology (LIBM)

ILE D'YEU, FRANCE

SEPTEMBER 2nd-14th, 1991

The course is intended to familiarize young scientists, especially of the Eastern countries,with new experimental concepts and approaches to an analysis of single events of the cellcycle and early embryogenesis presenting cell systems such as the sea urchin, star fish,ascidia, etc. At the same time it will offer for single cells the latest progress in imageanalysis, microinjection and ion measurements. It is essentially a practical course together

with additional lectures and discussions.

Faculty: B. R. Brinkley (Birmingham, USA); A. Lambert (Strasbourg, FRANCE);D. Mazia (Pacific Grove, USA); R. Nobiling (Heidelberg, RFA); C. Petzelt (He d'Yeu, FRANCE);

H. Sato (Nagano, JAPAN); G. Sluder (Boston, USA)

About 20 students will be accepted. The registration fee is FF 3000 (room and board notincluded). A limited number of fellowships is available, please apply as soon as possible(C.V., reasons to attend the course, and if you absolutely need support to attend the course).

Further information and application (please phone or Fax!):Prof. Christian Petzelt, Laboratoire International de Biologie cellulaire Marine,

LIBM, F-85350 lie d'Yeu, FranceTel.: 51.59.21.60 Fax: 51.58.39.12

THE CELL CYCLEEdited by

Robert Brooks, Peter Fantes, Tim Hunt, Denys Wheatley

Journal of Cell Science Supplement 12 1989Casebound, approx 300pp. $60 (£40) ISBN 0 948601 23 X

Contents:

Maturation promoting factor, Histone HI kinase and the timing of mitosis.Reports on yeast and higher eukaryote cycles from Nurse, Beach, Hyams,Reed, Doree, Mailer and Co-workers.

The role of cyclin synthesis and destruction in the cell cycle.

Reports from the Murray, Hunt, Whitaker, Cross and Karsentilaboratories.

The role of DNA damage and DNA synthesis in the control of the cell cycle.Reports from the Hartvvell, Newport, Stillman, Roberts, Laskey andHutchison groups.

Chromosomes; Centrosomes, Centromeres and Kinetochores.

Reports from Yanagida, Bloom, Hurt, Sluder and Glover.

The Cell Cycle is the most upto date analysis of new dataand ideas on cell cycleregulation by most of theleading researchers in thisrapidly moving and importantfield

This indexed volume is basedon the successful BritishSociety for Cell Biologyinternational meeting held inApril 1989 at St Andrews,Scotland.

JOURNAL OF CELL SCIENCEA major international journal of cell biology

12 monthly issues plus annual supplements (2 in 1991)

1991 subscriptions: Libraries - $870 (USA), £550 (Japan), £500 (rest of world)

New, lower price: personal subscription for individual use only - £75; $100 including postage and handling

24-hour ordering by FAX: (0206) 549331 - USA 44 206 549331

I would like to subscribe to Journal of Cell Science at the library • or individual • rate.

Please send me copy(s) of The Cell Cycle @ $60 • £40 • 1991

Libraries:

Individuals

USA

$870

$100

RastolWorid

£500

£75

Account No.

Name:

[ Expiry date:_

(PLEASE PRINT). Signature:.

Address:.

.Telephone:.

All orders toThe Portland Press, PO Box 32, Commerce Way, Colchester CO2 8HP, Essex, UK.Telephone: Colchester (0206) 46351. Telex No: 987275 BIOSOC G

orDistributed in the US by The Rockefeller University Press. Send prepaid orders to Order Service, The Rockefeller UniversityPress, Box 5483, Church Street Station, New York, NY 10249. All checks must be drawn on US banks and payable toThe Rockefeller University Press. No credit cards accepted on US orders. 391120

Journal of Cell Science Supplements

No. 1 Higher Order Structure in the NucleusEdited by P. R. Cook and R. A. LaskeyISBN. 0 9508709 4 3 234 ppProceedings of 1st BSCB-Company of Biologists (COB)Symposium £12 00

No 2 The Cell Surface in Plant Qrowth and DevelopmentEdited by K. Roberts, A. W. B. Johnston, C W. Lloyd,P. Shaw and H. W, WoolhouseISBN-0 9508709 7 8 350 pp.The 6th John Innes Symposium £15 00

No. 3 Qrowth Factors: Structure and FunctionEdited by C. R. Hopkins and R. C. HughesISBN: 0 9508709 9 4 242 ppBSCB-COB Symposium £15 00

No 4 Prospects In Cell BiologyEdited by A. V. Grimstone, Henry Harris andR. T. JohnsonISBN-0 948601 01 9 458 pp.An essay volume to mark the journal's 20th anniversary £13 I

No 5 The Cytoskeleton: Cell Function and OrganizationEdited by C W Lloyd, J. S. Hyams and R M WarnISBN: 0 948601 04 3 360 pp.BSCB-COB Symposium £19 00

No 6 The Molecular Biology of DNA RepairEdited by A. R. S. Collins, R T Johnson andJ. M. BoyleISBN'0 948601 06 X 353 pp. £40.00

No 7 Virus Replication and Qenome InteractionsEdited by J. W Davies et al.ISBN: 0 948601 10 8 350 pp.The 7th John Innes Symposium £40.00

No 8 Cell Behaviour: Shape, Adhesion and MotllltyEdited by J. Heaysman, A. Middleton and F. WattsISBN. 0 948601 12 4 449 pp.BSCB-COB Symposium £35.00

No 9 Macrophage Plasma Membrane Receptors: Structure and FunctionEdited by S GordonISBN. 0 948601 13 2 200 pp. £29 00

No.10 Stem CellsEdited by Brian I Lord and T Michael DexterISBN: 0 948601 16 7 280pp. £35 00

No 11 Protein TargetingEdited by K. F. Chater, N J. Brewin, R. Casey, K. Roberts, TM. A. Wilson and R. B. FlavellISBN'0 948601 21 3 270 pp.The 8th John Innes Symposium £35.00

No 12 The Cell CycleEdited by Robert Brooks, Peter Fantes, Tim Hunt and DenysWheatleyISBN: 0 948601 23 X 300 pp.BSCB-Joumal of Cell Science Symposium £40 00

No. 13 Qrowth Factors In Cell and Developmental BiologyEdited by M. D. WaterfieldISBN-0 948601 27 2 210ppBSCB-Joumal of Cell Science Symposium £30.00

U S $23 00

U S $30 00

US$30 00

SOLD OUTD U o 530 00

SOLD OUT

U.S.$70 00

U S $70 00

U.S.$60.00

U S $50 00

U.S.$65.00

U.S $65 00

U.S$60 00

U S $55.00

1984

1985

1985

1986

1986

1987

1987

1987

1988

1988

1989

1989

1990

This series of supplementary casebound volumes deals with topics of outstandinginterest to cell and molecular biologists

These are provided free to subscribers to Journal of Cell Science. They may be purchased separately from:

Portland Press Ltd, PO Box 32, Commerce Way, Colchester CO2 8HP, UK