john girard project opening doors. chemical “messengers” cells communicate by chemical...
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Cell Communication
John GirardProject Opening Doors
Cells communicate by chemical “messengers”
Animal and plant cells have cell junctions that directly connect the cytoplasm of adjacent cells
In local signaling, animal cells and unicellular organisms may communicate by direct contact, called cell-cell recognition
Cell Communication
Animal cells communicate using local regulators, messenger molecules that travel only short distances
In long-distance signaling, plants and animals use chemicals called hormones
Cell Communication
Local and Long-Distance Cell Communication in Animals
Local and Long-Distance Cell Communication in Animals
Local and Long-Distance Cell Communication in Animals
Communication by Direct Contact between Cells
Communication by Direct Contact between Cells
A signal transduction pathway is a series of steps by which a signal on a cell’s surface is converted into a specific cellular response
Cells receiving signals go through three processes:
Reception Transduction Response
Cell Communication
Overview of Cell Signaling
Overview of Cell Signaling
Overview of Cell Signaling
Receptors in the Plasma Membrane
There are three main types of membrane receptors:
G protein-coupled receptors
Tyrosine kinase receptors
Ion channel receptor
The Structure of a G-Protein-coupled Receptor
The Functioning of a G-Protein-coupled Receptor
The Functioning of a G-Protein-coupled Receptor
The Functioning of a G-Protein-coupled Receptor
The Functioning of a G-Protein-coupled Receptor
Activity: Pathways with Friends
Instructions
1. Form groups of 6 people each
2. Create space for your group to interact without bumping into each other
3. Rules:
Each person in the group will get a card
Do not let others in your group know what your card says
When prompted, follow the instructions on the card
The Functioning of a G-Protein-coupled Receptor
Activity: Pathways with Friends
The Functioning of a G-Protein-coupled Receptor
Activity: Pathways with Friends
The Functioning of a G-Protein-Coupled Receptor
Activity:
The Functioning of a G-Protein-Coupled Receptor
Activity:
Receptors in the Plasma Membrane
Tyrosine-kinase receptors are membrane receptors that attach phosphates to tyrosines
A tyrosine-kinase receptor can trigger multiple signal transduction pathways at once
The Structure and Function of a Tyrosine-Kinase Receptor
The Structure and Function of a Tyrosine-Kinase Receptor
The Structure and Function of a Tyrosine-Kinase Receptor
Activity:
The Structure and Function of a Tyrosine-Kinase Receptor
Activity:
A Ligand-Gated Ion-Channel Receptor
A Ligand-Gated Ion-Channel Receptor
A Ligand-Gated Ion-Channel Receptor
Hormone(testosterone)
Receptorprotein
Plasmamembrane
EXTRACELLULARFLUID
DNA
NUCLEUS
CYTOPLASM
Steroid Hormone Interacting with an Intracellular Receptor
Receptorprotein
Hormone(testosterone)
EXTRACELLULARFLUID
Plasmamembrane
Hormone-receptorcomplex
DNA
NUCLEUS
CYTOPLASM
Steroid Hormone Interacting with an Intracellular Receptor
Hormone(testosterone)
EXTRACELLULARFLUID
Receptorprotein
Plasmamembrane
Hormone-receptorcomplex
DNA
NUCLEUS
CYTOPLASM
Steroid Hormone Interacting with an Intracellular Receptor
Responses to Other Signals
Innerchamber
Outerchamber
–90 mV
140 mM 5 mM
KCIKCI
K+
Cl–
Potassiumchannel
(a) Membrane selectively permeable to K+ (b) Membrane selectively permeable to Na+
+62 mV
15 mMNaCI
Cl–
150 mMNaCI
Na+
Sodiumchannel
CYTOPLASM
Reception
Plasmamembrane
Cellwall
Phytochromeactivated by light
Light
Transduction
Second messenger produced
cGMPNUCLEUS
1 2
Specific protein
kinase 1 activated
Responses to Other Signals
CYTOPLASM
Reception
Plasmamembrane
Cellwall
Phytochromeactivated by light
Light
Transduction
Second messenger produced
cGMPSpecific protein
kinase 1 activated
NUCLEUS
1 2
Specific protein
kinase 2 activated
Ca2+ channel opened
Ca2+
Responses to Other Signals
CYTOPLASM
Reception
Plasmamembrane
Cellwall
Phytochromeactivated by light
Light
Transduction
Second messenger produced
cGMPSpecific protein
kinase 1 activated
NUCLEUS
1 2
Specific protein
kinase 2 activated
Ca2+ channel opened
Ca2+
Response3
Transcriptionfactor 1
Transcriptionfactor 2
NUCLEUS
Transcription
Translation
De-etiolation(greening)responseproteins
P
P
Responses to Other Signals
Signal Transduction Pathways
Protein kinases transfer phosphates from ATP to protein, a process called phosphorylation
Signal Transduction Pathways
Protein phosphatases remove the phosphates from proteins, a process called dephosphorylation
This phosphorylation and dephosphorylation system acts as a molecular switch, turning activities on and off
A Phosphorylation CascadeSignaling molecule
ReceptorActivated relaymolecule
A Phosphorylation CascadeSignaling molecule
ReceptorActivated relaymolecule
Inactiveprotein kinase
1 Activeproteinkinase
1
A Phosphorylation CascadeSignaling molecule
ReceptorActivated relaymolecule
Inactiveprotein kinase
1 Activeproteinkinase
1
Inactiveprotein kinase
2
ATPADP Active
proteinkinase
2
P
PPP
i
A Phosphorylation CascadeSignaling molecule
ReceptorActivated relaymolecule
Inactiveprotein kinase
1 Activeproteinkinase
1
Inactiveprotein kinase
2
ATPADP Active
proteinkinase
2
P
PPP
Inactiveprotein kinase
3
ATPADP Active
proteinkinase
3
P
PPP
i
i
A Phosphorylation CascadeSignaling molecule
ReceptorActivated relaymolecule
Inactiveprotein kinase
1 Activeproteinkinase
1
Inactiveprotein kinase
2
ATPADP Active
proteinkinase
2
P
PPP
Inactiveprotein kinase
3
ATPADP Active
proteinkinase
3
P
PPP
i
ATPADP P
ActiveproteinPP
P i
Inactiveprotein
Cellularresponse
Phosphorylation cascade
i
Second Messengers
The extracellular signal molecule that binds to the receptor is a pathway’s first messenger
Second messengers are small, nonprotein, water-soluble molecules or ions that spread throughout a cell by diffusion
Second Messengers
Cyclic AMP (cAMP) is one of the most widely used second messengers
Adenylyl cyclase, an enzyme in the plasma membrane, converts ATP to cAMP in response to an extracellular signal
cAMP as a Second Messenger
First messenger
G protein
Adenylylcyclase
GTP
ATP
cAMPSecondmessenger
Proteinkinase A
G protein-coupledreceptor
Cellular responses
Epinephrine Transduction Pathway
cAMPSecond
messenger
Adenylylcyclase
G protein-coupledreceptor
ATP
GTP
G protein
Epinephrine
cAMPSecond
messenger
Adenylylcyclase
G protein-coupledreceptor
ATP
GTP
G protein
Epinephrine
Inhibition ofglycogen synthesis
Promotion ofglycogen breakdown
Proteinkinase A
Epinephrine Transduction Pathway
cAMP as a Second Messenger
Activity:
cAMP as a Second Messenger
Activity:
Calcium Ions
Calcium ions (Ca2+) act as a second messenger in many pathways
Calcium is an important second messenger because cells can regulate its concentration
The Maintenance of Calcium Ion Concentrationsin an Animal Cell
High Ca++
Low Ca++
Inositol Triphosphate
Pathways leading to the release of calcium involve inositol triphosphate (IP3) and diacylglycerol (DAG) as additional second messengers
Calcium and Inositol Triphosphate in Signaling Pathways
EXTRA-CELLULARFLUID
G protein
GTP
Phospholipase C PIP2
DAG
Endoplasmicreticulum (ER) Ca2+
CYTOSOL
Signaling molecule(first messenger)
IP3
IP3-gatedcalcium channel
(second messenger)
G protein-coupledreceptor
Calcium and Inositol Triphosphate in Signaling Pathways
G protein
PIP2
EXTRA-CELLULARFLUID
Signaling molecule(first messenger)
GTP
IP3
DAG
IP3-gatedcalcium channel
Endoplasmicreticulum (ER) Ca2+
CYTOSOL
Phospholipase C
(second messenger)
Ca2+
(secondmessenger)
G protein-coupledreceptor
Calcium and Inositol Triphosphate in Signaling Pathways
DAG
Variousproteinsactivated
Cellularresponses
Ca2+
(secondmessenger)
Endoplasmicreticulum (ER) Ca2+
CYTOSOL
IP3-gatedcalcium channel
PIP2
IP3
(second messenger)
GTP
G protein
G protein-coupledreceptor
EXTRA-CELLULARFLUID
Signaling molecule(first messenger)
Phospholipase C
Nuclear Response to a Signal: The Activation of a
Specific Gene by a Growth Factor
Growth factor
Receptor
Phosphorylationcascade
Reception
Transduction
Activetranscriptionfactor
ResponseP
Inactivetranscriptionfactor
CYTOPLASM
DNA
NUCLEUS mRNA
Gene
Signal Amplification: Stimulation of Glycogen Breakdown by Epinephrine
Is Signal Transduction Important?
Androgen Insensitivity Syndrome:
Genetic male (XY) without androgen receptors
The gene for the syndrome is on the X chromosome in band Xq11-q12.
The gene codes for the androgen receptor.
Result: No signal transduction!
Cell Communication Free-Response Questions
19922. Biological recognition is important in many processes at the molecular, cellular, tissue, and organismal levels. Select three of the following, and for each of the three that you have chosen, explain how the process of recognition occurs and give an example.
a) Organisms recognize others as members of their own species.
b) Neurotransmitters are recognized in the synapse.
c) Antigens trigger antibody responses.
d) Nucleic acids are complementary.
e) Target cells respond to specific hormone
Cell Communication Free-Response Questions
19992. Communication occurs among the cells in a multicellular organism. Choose THREE of the following examples of cell-to-cell communication, and for each example, describe the communication that occurs and the types of responses that result from this communication.
a) Communication between two plant cells
b) Communication between two immune-system cells
c) Communication either between a neuron and another neuron, or between a neuron and a muscle cell
d) Communication between a specific endocrine-gland cell and its target cell
Cell Communication Free-Response Questions
20071. Membranes are essential components of all cells.
a) Identify THREE macromolecules that are components of the plasma membrane in a eukaryotic cell and discuss the structure and function of each.
b) Explain how membranes participate in THREE of the following biological processes:
• Muscle contraction
• Fertilization of an egg
• Chemiosmotic production of ATP
• Intercellular signaling
Cell Communication Free-Response Questions
20081. The physical structure of a protein often reflects and affects its function.
a) Describe THREE types of chemical bonds/interactions found in proteins. For each type, describe its role in determining protein structure.
b) Discuss how the structure of a protein affects the function of TWO of the following.
• Muscle contraction
• Regulation of enzyme activity
• Cell signaling
c) Abnormal hemoglobin is the identifying characteristic of sickle cell anemia. Explain the genetic basis of the abnormal hemoglobin. Explain why the sickle cell allele is selected for in certain areas of the world.