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Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee P, Gosden RG Fertil Steril. 1997 Mar;67(3):481-6.

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Page 1: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee

Isolation and characterization of primordial follicles from fresh and

cryopreserved human ovarian tissue

Oktay K, Nugent D, Newton H, Salha O, Chatterjee P, Gosden RG

Fertil Steril. 1997 Mar;67(3):481-6.

Page 2: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 3: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 4: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 5: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 6: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 7: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 8: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee

Chemical structure of sterols that activate oocyte meiosis

Byskov AG, Andersen CY, Nordholm L, Thogersen H, Xia G, Wassmann O, Andersen JV, Guddal E, Roed T.

Nature. 1995 Apr 6;374(6522):559-62

Page 9: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 10: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 11: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee

Kryokonservierungvon

Spermatogonien

• Spermatogonien reifen in Samenfäden anderer Spezies heran

• Ralph Brinster• Nature Medicine , 6,16

Page 12: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 13: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee

Präimplantationsdiagnostik I

• Cystische Fibrose• M. Tay Sachs• Duchennesche Muskeldystrophie• Fragiles X - Syndrom

Page 14: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee

Präimplantationsdiagnostik II

• Trisomie 21• BrCa I• Sexing• Numerische Aberrationen

Page 15: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 16: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 17: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 18: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 19: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 20: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 21: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 22: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 23: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 24: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee

Leben ist Elektronik.Den ersten Impuls zum

Elektronenfluß gab die Sonne, wodurch

Kohlenwasserstoffverbindungen entstanden. Deren Verbrennung

erzeugt ebenfalls einen Elektronenfluß, aus dem unser

Körper entstand.

Page 25: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 26: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee
Page 27: Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue Oktay K, Nugent D, Newton H, Salha O, Chatterjee

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dial follicles (Oktay et al., 1997). Ovarian cortical pieces (2 x 2 mm) were cryopreserved with the slow-freeze process (Table 2). After thawing, the tissues were par- tially digested

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Cryptographie Réalisé par: Jihen THOUIR Imen JENDOUBI Meriam BEN SALHA Marwa LAZHAR

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