isolation and characterization of microsatellite markers for the neotropical tree, pachira quinata...
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MICROSATELLITE LETTERS
Isolation and characterization of microsatellite markersfor the neotropical tree, Pachira quinata (Malvaceae)
Paul D. Rymer • Stephen Harris • David Boshier
Received: 4 November 2013 / Accepted: 25 November 2013
� Springer Science+Business Media Dordrecht 2014
Abstract Twelve microsatellite loci were developed
from a genomic library enriched for SSRs of Pachira
quniata, a canopy tree species of dry and some humid
forests in the neotropics (Central and northern South
America). Genotyping of 30 trees from one population on
the Pacific coast of Nicaragua was successfully performed.
The loci were across amplified in related species. These
microsatellites will be used to compare regional population
structuring and mating patterns in fragmented landscapes.
Keywords Malvaceae � Bombacaceae � SSR
markers � Neotropical forest
Pachira quinata (Jacq.) (Alverson 1994) (syn: Bombac-
opsis quinata) is a medium to large-size deciduous tree,
highly valued for its timber within its native range from
Honduras in Central America, to Colombia and Venezuela
in South America (listed as vulnerable by IUCN). It is
hermaphroditic, largely self-incompatible, and principally
bat pollinated (Sandiford 1998).
We aimed to characterize highly polymorphic micro-
satellite (SSR) markers to be used in future studies on the
distribution and abundance of neutral genetic variation, and
the estimation of mating patterns in natural populations.
Seed from one P. quinata tree collected from Nambaras,
Nicaragua (12�100N, 85�380W) was extracted using a
QIAGEN DNeasy plant mini kit to obtain total genomic
DNA. The microsatellite-enriched library was constructed
using the protocol described by Glenn and Schable (2005),
with minor modifications (McPherson et al. 2008).
Fifty clones enriched with the mixed probes and two hun-
dred and twenty AG-rich clones were ethanol purified and
sequenced using universal M13 primers and BigDye termi-
nator version 3.1 (AppliedBiosystems). Fifty-one of these
sequences contained SSRs (see Appendix for sequences), 27
of which were suitable to design locus-specific primers based
on primer3 software and NETPRIMER (Premier biosoft).
Twenty-two were synthesized and tested as unlabeled pro-
ducts, 15 were selected for genotyping analysis being labeled
with fluorescent dyes (6FAM, PET, VIC and NED; Applied
Biosystems) and, of these, 12 loci were informative (Table 1).
DNA extracts from 30 individuals from a remnant forest
in Nambaras (Nicaragua) were then genotyped. PCR
amplifications were performed in an ABI Thermal cycler.
Amplification conditions were 94 �C for 3 min; 30 cycles
94 �C for 30 s, 60 �C for 30 s, 72 �C for 45 s; and a final
extension at 72 �C for 10 min. Yorkshire Biosciences TAQ
polymerase, 109 buffer and MgCl2, New England Biolabs
dNTPs and BSA were used in all polymerase chain reac-
tions (PCR). Reaction volume 10 uL: 19 buffer; 2.0 mM
MgCl2; 0.15 mM of each dNTP; 0.2 ng BSA; 0.25 mM
reverse (R) and forward-labelled (F) primers (Table 1); 0.5
U of TAQ. PCR products were run with Applied Biosys-
tems GeneScan LIZ-500 size standard on a 3730xl
sequencer. Fragment sizes were scored using ABI PRISM
GeneMapper version 4.0 software (Applied Biosystems).
Estimates of genetic variation were calculated in GenAlEx.
Hardy–Weinberg equilibrium (HWE) and linkage dis-
equilibrium were tested using the exact test in Arlequin,
P. D. Rymer (&)
Hawkesbury Institute for the Environment, University of
Western Sydney, Bourke Street, Richmond, NSW 2753,
Australia
e-mail: [email protected]
P. D. Rymer � S. Harris � D. Boshier
Department of Plant Sciences, University of Oxford, South Parks
Road, Oxford OX1 3RB, UK
123
Conservation Genet Resour
DOI 10.1007/s12686-013-0097-0
with significance levels determined after 1,000 batches of
10,000 iterations each (Bonferroni corrections were
applied). Amplification of primers with other members of
the Malvaceae was performed once using the same PCR
conditions on two individuals. The PCR products were run
on a 1.5 % agarose gel and visualized under UV light with
ethidium bromide staining.
Twelve informative primer pairs were found (Table 1)
with up to 17 alleles per locus. The majority of repeat
regions found were dinucleotide repeats; however, two
trinucleotide were also characterized. Comparison of the
allele size ranges obtained with the expected size from the
clone sequence indicates that there is more allelic variation
in P. quinata than found in the single population. Loci
Bq30 and Bq23 were outside HWE (Table 1). None of the
132 pairwise tests of genotypic disequilibrium were sig-
nificant after Bonferroni corrections. The characterized
microsatellites were also tested for amplification with DNA
extracts of Pachira aquatic, Ceiba pentandra, Pseud-
obombax ellipticum and Adansonia digitata (Malvaceae)
obtained from Oxford herbarium (FHO). Cross-species
amplification was most successful with related species
(Table 2).
Overall, the variability recorded within a single popu-
lation of P. quinata, and the amplification success across
other taxa suggests that it will be possible to further
develop large-scale population genetic studies (Rossetto
et al. 2007). We are particularly interested in investigating
regional patterns of diversity and genetic structuring, as
well as, the consequences of population fragmentation on
mating patterns.
Acknowledgments This paper is an output from the Seedsource
project funded by the EC (FP6-2002-INCO-DEV-1) and from
research projects partly funded by the United Kingdom Department
Table 2 Cross species amplification of Pachira quinata SSR loci
SSR loci Pachira
aquatic
Ceiba
pentandra
Pseudobombax
ellipticum
Adansonia
digitata
Bq06 1 1 1 1
Bq08 1 1
Bq12 1 1 1 1
Bq16 1 1 1 1
Bq19 1 1 1 1
Bq20
Bq22 1 1
Bq23 1 1 1
Bq25 1 1 1
Bq29 1 1
Bq30 1
Bq31 1 1 1 1
1 = Amplified fragment of correct size
Ta
ble
1A
des
crip
tio
no
fP
ach
ira
qu
ina
taS
SR
loci
wit
hp
rim
erse
qu
ence
s,es
tim
ates
of
gen
etic
var
iati
on
,an
dte
stfo
rH
ard
y–
Wei
nb
erg
equ
ilib
riu
m
Nam
eF
wd
-seq
uen
ceR
ev-s
equ
ence
Siz
eR
epea
tN
aN
eI
Ho
He
UH
eF
a
Bq
06
CA
AC
TC
CG
TC
CC
TT
AT
CC
AA
GT
GA
TG
CC
CC
AC
TG
AT
TT
CT
44
7(G
A)1
26
3.5
41
1.4
05
0.5
17
0.7
18
0.7
30
0.2
79
ns
Bq
08
CG
GT
GT
CG
AT
TG
TG
AG
TA
TG
GC
CG
AT
GG
GT
TG
GC
TA
GA
AT
A1
88
(GA
)23
42
.15
10
.91
90
.56
70
.53
50
.54
4-
0.0
59
ns
Bq
12
TC
TT
TC
GG
AC
CA
AG
TT
GT
GA
TG
GG
AA
GA
CC
AG
GA
CT
GA
TT
25
5(G
A)1
38
3.8
54
1.6
16
0.6
33
0.7
41
0.7
53
0.1
45
ns
Bq
16
GC
AA
GG
AG
GA
GC
AG
GA
TA
GT
TC
GT
GA
TC
TA
AA
TG
CA
AG
AG
CA
20
9(G
A)2
08
3.1
14
1.5
64
0.6
33
0.6
79
0.6
90
0.0
67
ns
Bq
19
TT
CC
TA
TC
CT
GC
TC
CG
CT
AC
AT
CG
AG
CC
AA
AC
CT
TT
CC
TT
14
0(G
A)1
81
71
1.1
11
2.5
91
0.7
67
0.9
10
0.9
25
0.1
58
ns
Bq
20
GT
TT
CA
AA
CG
GA
GA
CA
TC
TT
GA
AG
GA
AA
GG
AG
CA
TA
GG
CA
AA
18
4(G
A)3
CC
(GA
)13
83
.16
31
.43
20
.60
00
.68
40
.69
50
.12
3n
s
Bq
22
TG
AT
TT
TG
AA
CC
AA
CC
AG
GA
GG
TG
CT
TC
AA
GG
AA
AA
CG
AA
19
7(C
A)1
18
2.1
61
1.2
58
0.4
33
0.5
37
0.5
46
0.1
93
ns
Bq
23
TT
GA
GT
GG
AA
GG
GG
TC
TT
TC
GC
AA
CA
TG
CG
TA
TG
TC
CT
GA
32
2(G
A)1
81
27
.36
42
.19
90
.40
70
.86
40
.88
10
.52
9*
**
Bq
25
AG
GT
CA
AG
GG
GT
TT
GG
AG
AG
AT
AT
GG
AG
CC
CA
AT
GA
CA
GC
19
6(A
AT
)6(A
AC
)51
37
.69
22
.24
30
.83
30
.87
00
.88
50
.04
2n
s
Bq
29
TC
TC
AC
CA
GT
AA
CC
AG
TT
TG
AT
GT
TA
AG
CA
TT
GC
AG
GA
CA
GG
AC
24
3(G
A)7
AT
(GA
)11
83
.98
21
.66
60
.83
30
.74
90
.76
2-
0.1
13
ns
Bq
30
CG
GT
TC
TT
CA
GG
CT
CA
TC
AC
CT
GT
CC
AC
AA
CG
AC
CA
TT
AA
AA
28
2(T
GA
)14
11
7.1
15
2.1
23
0.8
67
0.8
59
0.8
74
-0
.00
8*
**
Bq
31
TT
CT
GA
AG
CC
AG
CA
CA
AC
TC
CC
TC
CC
AC
TG
AC
CC
AG
AT
AA
24
3(G
A)1
59
5.9
80
1.9
00
0.8
33
0.8
33
0.8
47
-0
.00
1n
s
ns
no
n-s
ign
ifica
nt
**
*P
\0
.01
aC
hi
squ
are
test
for
HW
E
Conservation Genet Resour
123
for International Development (DFID) for the benefit of developing
countries (R5729, R6168, R6516 Forestry Research Programme). The
views expressed are not necessarily those of DFID.
Appendix
[Bq06
AATCNCCCGAGNACCTGTATNTTCTCAATGAATCC
TCACCAGACTGAAGAAAACTCAATCATGACAACA
TATGTAAGAAAAGGAAAACAAAGAAGGAATGATT
CTCACATTAATGAAAAACGGAAGCAGAAGAAAAG
ACTGACCCTTTTGGCATTTAAAACTTGCTCCCTTC
GGTTTGGCTCAACTCCTAATCCTGTGCATGAGACT
AGAACAAAATCTGTTTCCTGTCCAGTCTGCAGAA
CATCAGGGTAAAATTATTTTAGAATATCTGATCTT
CTTAATCGTATAGCATAACGACTAGGTAGTAACTT
CAACTCCGTCCCTTATCCAATCTAAGTTCCACATT
TTACTTGGATAAACATATTCACATTCAAATTAATA
ACCAAGTAGCTGACAAGGAATCACTATTAACAGC
CATATTGAGAACATGGAACATCCATAAGCATCCA
TAAAATGATAGTATAAACTATAGAGTGGTGTAAA
AATATGAGAGAGAGAGAGAGAGAGAGAGAAACT
GAAGATCCTTCTGTTGATGATCGAAGAGGAATAT
ACATAAAAACACTTACCGGCAAGGCTTTTATATAT
TCCAATATAAGCTTGAAACTCCGGGGGTCTTGCTT
CATTCCTGCTGTTCCTTCAATGGGCCTCTGCAGCA
AGAATAATACATAGAATTAGAAGCATTAAAAGCA
GCGAGAGTTATCATTTTGAACAAGGAGAGTAAGA
AATTTATAAATCATCAGAAATCAGTGGGGCATCA
CACTTCTGACGGCAGCGCTACTACAAAAATGGGT
GAAGAACTTTTACTGTAATCATCATTTNGGACTT
GATGACACCATGTCTTGTCCAATTAGGAGACGATA
AACAAAAACAATTACTAGTTTTTTGTTCGCAGAGT
[Bq07
ACAGCAAGAGAGAGAGAGAGAGAGAGAGGGCGA
GCGCCGGATGCACATGCTTCGCATTTAGTTTGCTT
AGGCACACATCAGACAATACGGTGCACCCCATGT
GCCTTATTTAAGCCTCTTTCTCCTTTCTTCAGCTT
CCACACTTCAATTCGTTTTCCCTTTTCCAAAATAA
ATAAATAAAAATCTATCTTTTTTCGAAGCCCAATT
GTTAAATATGTATGTGTGTGTATATATATATATAT
ATCATGAACCAACCAAGTATGTAGCAATATCTTG
GCATCTTTAGCTTGCCATAAACTAATTACTTCTTCT
CAAAGGGt
[Bq08
CGGTGTCGATTGTGAGTATGGTATGTTCTTTTTCTT
TCCCTTTTGTTTATTTGCACAAGAGAGAGAGAGA
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
AACATATTGTTTCTACTAAATAAACAACTAAAGA
AGTCAACAATCTGAATCAGTCAGCTACCTGCTATT
CTAGCCAACCCATCGGCAAACAGACAGCCAAAAA
TGAATAAATTGGTCATCTCTTTTCTATAGTTTCCGT
TCATTTCGTTTATTCTCTGTCTAATCTCTAGATTAT
AAGTGAAGCTACTAATTCTTGCAAGAATATCCAC
GGGATAGGCAAATGCAAAGTATTTCCAACAAGGA
TCGTTACAAACTTGGACGACGT
[Bq09
ACAATCATATTTGAATGGAAAAATCGTAACCCTTT
ACCTATATTGAGATAAGAACCCTATAATGGTAAA
GATGCCCCAATCAACTATAGAAGGATTGATGATA
AGTCAAAGATGTTTTTCAAAAACATAATCTAGTTA
ATGTGTTGTGGAGGTTGAAAGGAATCGAAACAGG
GTTTTTTCCCCCATATTTTGCTGTCAACGACAGCCG
GCGGCCAGCGGAGGCATTCGCCGGTTTCTGGGAAT
TTACAATTACCGGTTGAAGAAAAGAAGCTATGAG
AGAAAGAAACACAGACAGAGAGAGAGAGAGAGA
GAGAGAGAGAGAGAGAGAAGGAATATCTGAAAA
TGACAGGAAAGGAAAACTTCAGGTTTTTACAATG
ATCAAAACTCgCCGTTTCATTAAAAAAAATTGCAA
ATTAAGGTATATATTTTTATTACTATTTGCTGTCAT
TTCTATCTTTTATTTTTTATTTTTTTAATTATCACGT
GACTATTTTCGC
[Bq10
ACATGCATACACACACACACACACACCCACACAC
ACACACACACACATATATATATATATGGAATAGC
TTATACAGAAATCACATCGATCTCCAAATATCAAA
CTACCAAACCCATAGTGGTCTCCTTTTATTTTGATT
CAACCAAACGTAGCACACATGGCTGATATATCGAA
TTCCTATATTTGTTTAATAGCACTCGT
[Bq11
ACCGACATAAGCAAACAATCATTCACTCACATGA
TCCGTTGGCCTTGGCCTCTTCAATTTTTTACTCTC
TCACCTTTCTCGTTAGCTAGCTTTGTTTGCTTTCT
TCTTCTATTTTAAGTCCATAATTTAGTCTCTTTCT
ATCTCTTACGCCACCTTTTTTTTTTTTtGGAgATTT
tGGtGACtCGGtCCCTTTCgAATtACATTTtCtGGGTT
TTtGGTTTtGTtGGtGAATTtCGTTtAAGCGTTGtCAG
tGtCCGtCAtGAAtAGGGtGAACtGGGAAAtGGCATG
ATAACAgCGGGTtCCAAgAAAGGGtGGAGGCGGAt
AAGGAAGCAGGGGCtGTtGGTTTtCCAGTtCTtGTtG
tGGtCGTTTTANtCCAAtGTTTATtAAtGCtCTTTCCTt
GGAttCTTTCtCtCtCGTTtGCCtGTTTtCTAATTtAAAT
TTTtAAtGTTTTTtCCTTTTtgANnnAAAAATTTtCtCC
Conservation Genet Resour
123
CATTTCttGGCGTTtGCATtCGGTTTTtGATTNCNTTT
tAttCAAGCTAACAgAGAAAGAGAGAAAGAGAGA
GAGAGAGAGAGAGAGAGAGAGAGAGAGAAAGA
GAGAGAGAANCCTTGCTCGTNGATNCATGGTNTG
GGNTGCTCCNCAACCACAAGGAACTTAGGCAAA
GCTTNGAGAAAAGAACAAAATGAAGTTTATGAA
GCTTGGGTCGAAGCNTGAnGTCtTCCAAGTTGATG
GCAAATGCATCnGGTAAGATGGAGTTCTCTCACA
TTCTTTTCTTtTATAACAAgCCAGGaCAAATGTTCT
TTATNGTTGCTTCCTNTAAGCNGTTTCTTAACATC
TTGTTATCTTGTTNTGATTTGGTTTTGCCTCTCAT
TTTGTTTTCCGTTATGCTGTCTGCTCCTGACATGT
TTAATNGATATAAATTTTCCTTTATATTTTGCATG
GAAATCCGTATAGTTTTTGAGATGCAAGTTCATT
AGCTCTTGCTTCAAATCGATTTTTTACTGAATATG
TNTANGCTTATTCTATTTTGTATTGCTAGACTTGT
GTGT
[Bq12
ACAAATTCTTTCGGACCAAGTTGTGAGTCTCTCTC
TCTCTCTCTCTCTCTCTCATATGCACAAATGCATTG
GTAAGAAGAGGAAAGTCGAGTTAGTTTTTATTTAT
AGTGCAAAAAGAAGGAAAACACAGCATGCATGAC
TTTAATTTGCCCGGATTTTTCCCCTTGAAGGCCAT
TTGCAATTGGGACTTTTAAATTCAATGTGTTGAGG
ACTGAATTGGCTCAATTTGAGGCCCATAATAAATC
AGTCCTGGTCTTCCCACCGGCCGGCCTATTGATCT
CATTATCTAACATTATCTAAAATGGATTAGTATTT
GGATTACTTCATATATTGCACATTACTTATAAAAA
TTAAAATGATTTGAATTTAAAATAATTCAAATTTT
AAATAATTCAAAAATAAAATGATTCAAAACAAAA
ACGATTCAAATCGAAAATAAAACAAAAATTTAAA
AGCTTGAAATAAATTAATTCAAATTAATCTAAACA
AAAATCAACTCGATCAATCTATTTGATAGTTCTAC
GTGCAAAGATTTCGCTGTTTTTGCACGTTAAGATA
GTATTGATTGTGATGATAATTAGATAAATCATACA
TATCAATTAGGATTTAGATACTCTTAATTGATAAA
AATAAGCAATGGTCTCTCAACATATTTTCATATAG
TGGTAAGAGTTATCCAGAAGG
[Bq13
CATTCTATAACATCCATTCCTCATACCTTACCTCA
CTCACATATGACCAAGGAAGCATTCGGTCAACCC
TCATCATCATCATGATTTACCTAAATACATTTCA
ACCTTTTTCCACCTGAACCTTCAATTCCATATCA
AGATATGTTTTGGCTAGAATGGATAAAAACCAA
AGCCTAGCTAAGGGTGGGTTTCGGCCAAACACTT
CTTACCTAAAGGAATTTGAGTTTCTAAGCTTTTC
ATGAACACATATTCATCCCTTACTCAATACATGC
ACACACAAGAGCATGAATGGCATATGGTGGAGA
AGGTAACATGAACGGGTGGTCGGAAGAGGAGATC
CTTACCTTGGTGTTAGGAGGCTCACGACAACACTA
AGAAATGGGAATAAGCTTTCGACCAAAGCTTGAA
GATAGCTCTAAAAACCCAATCATTGAAAACCGTG
AGCAAGGATAAGAACTTAGGATCGAACTAAGAGA
TTTGAAATAAAATTTAGATGAAAATATAAAGGAG
AGAGAGAGAGGAATAATTTTTTCTCTTGGAGCTTA
CCTTAAATCTCCTTGGAATGATGTGTATGGGAGCT
TGAAGAAGCTTGATGGAGGTGGTAATGGAGGTAG
GAGGAAGAAAATGGAAGAAGAAGAAGAAGAAGA
AGAGAGAGAGTTAGTGGAAAATGTTGGAAAAGTA
AAAGTAGGTTAAAATGATGTGAAGGAAGTGTGTA
AGAGAAGATTGGTATCTTGCATGGGTTGGTGTGT
GAGTGGGATGGGAGAGTGTGTGAGTGGGAGACAT
GAAGTGATAGGTGTAAGGGTGAGTGGATGTGTGT
AAGAACACATGTCACATCATGTCTCCTTATCGAAA
TTTATGCTNGGAGCCAAAATCGAAACACGAAGAA
ATCATCGTATTTGACTCGGATTAAACTCTCAAAAG
TTAATTTAATATTTTTGAGAATATTTGAAAAGATC
AGGTTCGAAAAATCTCACCCGGATCATTAATTTGA
TTATTTTATTCTCAAATAACCTTAACAAGGCTTTA
AAGAGAATAAAATCGATTTAAGTACGAAAATTCA
TGAATCTTTCCTGGAGGTGTTACTGTTCACGAAAA
TAAGGACAAAACTATTAGGTTCAATTTTTGAGGCT
GTTTTTACAGTTCGACACACAAATCGAGGT
[Bq14
CATTGATATTCTGGTCCTCATAAAATATATTAGTG
TTT ATGTTCACATTTTTTGAATGACTATTTCTGTTA
ATATATTTATATGATGCGTATGAAGGATTTTAAGT
CATAATAATATTCATTTACTAATAATTAAAATTAA
TTAATTTTAATTTTTATGTCATATTCATTATCTTAT
TGTTAATATGGTTTTCTTATTTGTTTATTTTTTCCA
ATAAAAAATTTACTTATAAAAATGATATTTTCAAT
AACTTTCAAATAAAATATTTTATTAATTTTATCTT
CTTTTTCTTTTTCTTCTTCTTCTCTCTCTCTCTCTCT
CTCTCTCTCTCGCTCTTTTTTCCTACTTCATTCTTTT
TTCCCTTTCTTTCCCAAATAAAAAATAGGTTGCGA
AACAATTTTGAATTAAATTTCAATTTCATTAAATT
AAGGTTGAACTAAAACTTCGTTCAAGTTTAATATA
TAATTATAGATTATAAAAGTTATAGACATATTGTT
ATCGATTCATTCCATAAAACATATCTAGAAAATTA
ATTTTTACCGATTTAAAAACTGTCGATTATAGGTG
ATATGAGTTGATTGATGCTTCAATGGTGAACTTAT
AAGATCATAAAACCTATAAGATCACAAAAATTAG
ATAACATCTTAGTTTATATTGTGGGGTGCATCCTG
ATACTATATTTTGGGGtGCAtCCTGATTCTTAGATT
AATCAAGTGATTCAAAAAGTAAAAGTATACTAAG
ATTAAGCTCATACCAAGTGTATGTCACTTGCATC
TCTATATACAACGTGACATGT
Conservation Genet Resour
123
[Bq15
ACTTCATCGGCTAGCCCAAAAACTCCATACTCATA
ACAGAATACTAGGCAAGGCCCAATAATAATTAAA
AAAATTAAAAATTAATTGTTGGACTCAATAAAAA
GGGCGGTCCTTAGTTCACCCAATAAAAGGAAGGT
TACAGAAGCTGCGTCGCGTTAGTGGGCAAAGAAA
GGTAGCTATCTAGGGTTTCCTTTTTCTTTCGAAAA
TGATTTACGACGTAAATTCCCCTCTCTTCCGCTCA
TTCCTCAGCCAGAAGGGCGGCTCCTCCGACAAG
AGGTTACTCTCTCTCTCTCTCTCTCTCTCTCTCTCT
CTCTCTCTCTCtCTTGTACGGATTTTTATGTTCATA
TTTGCGTTTTTTTTGGTCTGTCTCAGTTTCAATGTG
ATTAATTTGAATAATTATATTCATCGGGATTGAGT
AACTATTAAACGACGACGAAGTTCTTGATATGAA
GGTTTTTGTATAAAGATTAAAGCTGCTCGAGAGG
GAAACTTGTTTATTTCATCTACTATTCGGTTACAGA
TTAGAACAAAAGGTTTATTTTCGAAAGATTGTGAT
TGTAGACAATATGAATCTGATGTCTAGCATAAGAT
CACTTGTGTCTAATTTGTTTGACATATGTGGTTAAC
TTCTATGTGCACGGT
[Bq16
ACCTTGAAACCTTTATTACTTGGCAGGCAAGGAGG
AGCAGGATAGTTTTGTCTTAAAGGTGCCCTTTGCT
TTGGGCATATCTTTTACATTTTGCTGTTCTTATATtG
GGATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC
TCTCTCTCGGCATCCGATCAAAAATTTGTTCCATA
AGACTGTCTTCCAAAACCTAGAATTTAAGATAAAA
TTGCTCTTGCATTTAGATCACGTGAGCCTGCCCTAG
GTGCTTTAGGCATAATTTATGTTATCTGAACTCGA
AATAAATGTCAAATTTAAGTATGCGT
[Bq17
ACTTCGACGTTGAATACGACCCTTTAGCCGCAGCC
TCTGCTGCTACTGCTTCTTCTACTACCGTTGACGT
GGACATTGCAATTGTTGAAAGCGAGAGCTTCGTA
TCGACTCAAGGTTGGGACTCGGATATGGTGGTGG
ATTATAGGATAAACGAGGAGGAGTTTCATAAGAT
TAGCTTGTTGGATTGTGATTTTTTTATTCGGAAGC
CACCTGACCCTGATAATGATGTTTATGATTTCAGG
GAGGTTAGTGAGCTCTTGTTTTAGTTCTTTTCTTA
ACACACACACACACACACACACACACACACACAC
ACGCGCACACACACACGCGCACACATATATGTCA
TGGATTTTGGTTAATTTTTTGCTGCACTTCAAtTGT
TGtGtTAATTTTGTTAGCCTTTTGGATGtGGGtTTTA
CTTAAATTCTGTCTTAAAtTATTGTGTCTGGATTTT
TTTGATGTTATTTTTAAtGGTTTTTTGGGtTTTTTTT
TTTCTAGTAAAAAAGATTTCTGTCATTCATTAAA
AAGATGAACAATATGAGTATATAGTAGAACATA
TTAAATCAGGGACATTCTTGAGTTCGAAACATGTC
TTCCTGAATATTCAAACCATATTTTTGCTGGCACA
TGAGAAGCTTCATTTAATGGAGGCCTTGTGTGTG
AAAACCTGCATAAGTGGATAGCTGCTTTTCTGCCT
GGCTTCATTCACATAAGGTCCAATAGGTGAACAAT
CTACTCTGTTGATTGAATCTGTTTCATGCCTCCAAG
CACTCTCTCTTCGAGGATTATTCTTTGATAACCCTT
TTGCACGCTCAAGTGCTCTCTCACTGCANCCGTAA
CTTCTGGt
[Bq18
ACACACCTGACACAGCTTTTACAAAAATTCAAAC
AAAACTCAGTTTTTCTTTAGAACCCCACCAAACCA
ATTGAGACAGAATCACTTTAATCACCTCCCATTGA
GAAAAGTTAAAATCCTTACCATTTTTCACCGCTTC
AAAGAGCAATAATAATAAAAACCAGATCTTGCTC
TTGCGAAGTCCTAAACTTTTGGCCATCGATGGACT
GAATCAAATAAACAGAAAAGCAGAGTCATTTGCA
TTTTCTAATCCTTTGACAGGCTTATATATGCTACTA
TAATAAACAATTTTGAAGTTCGTTAGATAAAAAG
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTGA
CAAGCAAGAGGCTGAATTAATTTTCATTTCTGTTT
CTTCAGGTAGACAAAGTTTGCTTTTGGATGCACAT
TTCTGTAACATTTGATTTGTTACAATGAAGATCAA
ACAACTATGGGCTTAAGTAGTTTCTGTAGAATTTT
GTTTTTCATAAGTGAAACATGCACTTAATTGAGGG
AAAATAACTCATCTGATTTGCATAGCTTAgTCACG
AACCTGCTACCATTGCAGTGCGTGAGGATTGTATG
GGTGTGGCTGGTAAATTCAGCCTGCCTTGTTTGTG
GGAAGCAAAAAGCATGGAATACTGGTGATCCGTC
TCnAGGAAGCAATTAGC
[Bq19
ACGCAGCTACACTATAGCAATAGCCAATCCTACA
TACATATCAGGTGTAAGCTGATCACAAACATTCAT
CCTCAGATTTTGATATCAGAATTCAGAAGGGTGAG
AAGAAGAGTTACACAGTGAAGATTACAGAGCGGT
GTCACCTAGTTCGTTATATTGCAAAAACAAAATGA
GTTAAATTTTTTGATTTCAAAGTCACTTTATAATAA
TTTTTGGATATTTTTCTTTTATTTTTCATTTGCCCTT
CTTTGGATTTGATAAAAATAATTTTGGACTTCTCC
AAAGGCTAATCTGAGGCCTTTTTCGGCTTAACTAA
ATTAGGCTAATGAAATAATTATTTGGGCTTTCAGA
GTCCATAAGTGTAAGGAGGACCAGTGTAGAGCAA
CGTTAAAAACCCGAATCTTAATCCGGTCCAAACAG
TAAGCTAAGCAAAGTAACAAGGCCATGGGCTCCA
ATCCTAGGGTTAAGGAGAANCTCATATAAATAGC
CACTATCACTTGTTTCCTATCCTGCTCCGCTACCTC
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
GAGGAAGAAATGGTGTCTCTTAAGCTCCAGAAAC
GGCTCGCTGCGAGCGTTCTAAAGTGTGgGAAAGG
Conservation Genet Resour
123
AAAGGTTTGGCTCGATCCAAATGAAGTCAACGAG
ATCTCCATGGCCAACTCCCGTAAGTCTTCCTCCAT
TCTCTCTTATCAGTGCTCCAACACTTTTTCATTGTC
GTTATCATCAGTTTTAATTTTTATACACACACGCA
CACACACACATATATATGTATACATAAAACTGAG
GTTTAAGCTGCTTCTGGAAAATGAAAGCTGAAAAG
CCGAA
[Bq20
CGTTCGGTTTCAAACGGAGACATCTTGAATTTACT
CGCATTTCTCTCTCTCTCTCTCTCTCTCTCTCTCCC
TCTCTCCTCCGAGAAAATAAGAGAAAGTGGTAAT
TCCAAAAGCGTAAAATAACTAACCTTCTACAGTT
CTTCTTTCTTTCTCCTTTTCTGTTGTTTGCCTATGCT
CCTTTCCTCCGCAGCAAGCTTTCATTTTTCCTCTTT
CTTTTCCTCAGTCTTCCCCTGTCTCTCTGCTCTGTA
ATATTAGCATTTCTTTTCTTTAATTTTTATTTCTTTT
TCTGATTTTTGATTATGTCTTACTTTTATTTTTTTG
GTTTAATTAACATTTAAATTAATTTTTTAAACGAG
TGTTGTAATTACAGTGACTAAATGACACTGTAAGT
AGCCGTTGGAATTCGTCAACGATCCGAACGGGTC
TTTCATTGCTTTTGAAGCTTGGAAGTTGACTTGCA
ATATTAGTGAATGATTCAACTTTAACCTTGAATAA
ATCTCAAATAAGGATGAACCGAAAAAGAACCAAG
TTACTCATTGATGT
[Bq21
ACTTCTGGTATTTAAGCCGATAATAAAATAGAAA
CAGCCGTTTTGGGATTCGAATTTTATACCAGTTCA
ATGCTTATATTTGTTAAAGGCCAATTCAAAGCAT
GCTGTAGCAACTCCAGAGAGGAAAAAACCATTGA
AAACGTCAAGAAACTATGCAGAAAACATCTTACC
CTAAAGTAAAAGGACAAAAGAAAAAATAAGAAT
CAATTGGCAATGAGCAAAGGGATTCAAACCCGA
GTCCAAGAGACCCTTTGTCATGATTTAATCAATC
AACACAATTTTTTTGGGCAAGATTTGTCAACAGT
TGAACCAATGGAAAAGAATGGAATAGCAACTAGT
CTTTCAAGAAATGACTATGGGGGCAAGAATGCC
ATGGACTTAATTCAATTAGTATTTATTAGTTGTTA
CCATCAAGAAGGCCATAGCTATATGAAATTATTAT
TGCGTGATCAATAATCTAAGAAATAAAGACATTC
AGTTGAAGTGGGAAATTAAGTGAAGTTTGGTCAA
CAAATAGTGTATGGTTCTAGGCGTGGTAAATTCT
TTAATAAAAGAACCAAAGTTTGACCGCTCCTTA
CAGTTTTCTCTCTCTCTCTCTCTCTCTCTCTCATAT
CTAATACGCAGACAAAGGATAAGCTTCATATTTAT
CCATAAGATCGTTGTGCATCCTACTGACAATTATC
TTCTATTTATTGATGTTCATCATCTGGAGAGGTGC
TCGGTAAGTGTCATCTTATTATCATCATACAAACA
TGATGGCTAGGCTGAAATTTATCAAACCCAGTTTT
TTATTTCAAATTCTGGTCAAATGTTCTTTGCCTGT
ATCAGAAAAGCTACACCATCTATGACTTTCATGAC
CTCTTTGTCAAACTGCAGTTAAGATAATGGCTCTG
GAGTAATGAGTTTAGCTCTTAAGCCTTTATCTTGC
CGGAGTTTGTAGATANNNTAAAGAAGAA
[Bq22
ACAGCTGTGGATACTTTAAAAGTGTCCTTCTACTT
AGGTCCTTCTCGAGGATACCAACAACAATTGTAG
GCACCGATGTGTAGATTACGGAATACAGCACACT
GCTCCACTCATTGATTGCAGTGGTCAAAGTGAAA
ACAAGTAAAGAGCACATACCTGCAAAGAACATGG
TAAAAGCTAAAAGTGCTCTTCTACAGATGAAATTT
CACTTTAATTGACCAAAAATGTGAGTCAAATTCTA
TTCCAAATTAAGTGTAAATTATGATTTTGAACCAA
CCAGGATCAAAATTCAATTATCTTTAACCTAATTG
AGCAGAGTATTTTACTTTTTTTATGTTTTGCTGCAT
GTAGGTTAGTCTAAATCACTAATACAAAAGGAGT
AAAAGATTAATCATACACACACACACACACACAC
ACATAAACTATTTTAGTCCGAATATTCGTTTTCCTT
GAAGCACCCATGTCCAACAGCAATACATATTCAC
ATATGGGATGAGGGTATGATTCTCCAAATATATGA
AAACTTGGAAAGAATTGAGCATACCCCTGTCGGA
CACATACTTGTATCCAACACTCATCCCTGAGTCGA
GTAACGTAGTATATAAACATGCAATGATGATATTT
TACTGTCTCAACCCCACTATTGGCCTGGTTAGTGC
CAAAAGATGCAGTTGATCTTAGGTGAAAATTAAG
GCTTGCTTAAACCAACAAGCTCAATGACCTAGGTA
GGTTGAATATCGGCATTTAGT
[Bq23
ACCCATACATTTGGAGGACTTGGACCTACTTGCTT
TCATAATTCTAGTAATTACTAAATATTACTATGAA
TCACTATAAAGAGGTTGTCAAAGACATTGTATTCT
GTCTATTTTCATATATCGTATGGATCAGGAGTTTC
ATTGTTACCATGTGCAATTTATTTATACTGATTGC
TGAATTTTCTGCAGATATGGTCAAGAATAAAGGA
TTCTTGCCGTCAGTTCCTTCAGAAATTGCTGTTC
AGAGGCAACAAATGAAGGAAATAATATTCTCTCT
ACTTGATGCATGCACGGCCAATGATCCTGCTAGTA
ATGTCAAATTTGATGTAGATGCAATAATTGCCAAT
CCTCCTGCATATGGtTAGGTCTTATTACCTCTTTTT
CATTTTTAAGCGGGATGTTTTTGTCTAATGACAAT
TGATCATTTTGAGTGGAAGGGGTCTTTCTGTTATC
TCTTTCACCATCCTGCATAGTCTGCAATATTTAAC
CTAAAGAAACATTAGATGTCCATTTTGTTGTTCTA
CATACTCAATGTTCTCTATATAAATCATTTTGGTA
TTCTAATGCTTGAAATTTGTGGACTTCTGCCCCTC
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCATG
TGTCTAATGgAAAAATACTTGTTCAGGACATACGC
ATGTTGCAGAGGCACTAAAAGT
Conservation Genet Resour
123
[Bq24
ACGGGTTCCTCTTCCCAAATCTATCATCCCGGGCT
CTCGTAGGTTTCCCTGAGAACTTCTCTGCTCTGAG
AGCCACCTGTTATTCTATAACATCCATTCCTATCA
TAACTTAAGCCTTTCTTGATGGTTTTCGATCATAA
GGGAAAAATAAAAGAGGAAAAATGATTTCTAAAG
TGTTTAGACTATTATTAACCTAAGCTAAGTCCATT
TCACGGCTGCCATGTCAAAACCCAAAACCTTAATC
ATGAAGATGGAAAATGGCATGACATGAGAAGAAA
AGTGAGAAGTGAGGTTTCACCTTGAAATGGAGAA
GAGTATGGCGACACAAAGCTTGTTTCACCCTTGAA
AGTTCAAATCGAAAACCTTAGACAAAAGTTACCA
TGGAATCGAAACATGAGGATAGGAAGATCAAGAA
TGGCGTAACATGGTGAAATAGATGAGAGAAGCGT
TACCTTGAGATGGTGAAGCAAGTGGCGGTAAAGA
TGACTTAAAGCTTGAGAATGAACATCAATGGTGG
TTATGGAAGAAGGAGAAGGAAAATGGAAGAAGA
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA
AAGAAGATAGAGGAAAGTGTTGGAAAAGTAAAA
GTAAGTTTAAGTGATGTGAAGGAAGTGTGTAAGA
GAAGATTGTCTTGCATAAGGTGGTGTGCAAAGTG
GGATGGGAGAGTATGTGAGTGGGAGACATGAAGT
GATGGGTGTAAGGGTGAATGGATGTGTGTAAGAG
CACAAGTCACATCATGTCTCCTTATTAAAATTTAT
GCTTGGAGTCAAAATCGAAATACGAATAAATCAT
CGTATTTGGCTCAGATTAAACTCCTGAAAGTAAAT
TTAATATTTTTGGGAATATTTGAAAAGATCGGGTT
TGAAAAATCTTACCTGGATAATTAATTTGATTAAT
TTATTCTCAAATAACGTTAACAAGGCTTTAAAGAG
AATAAAATCGATTTAAGAACGAAAATTCATGAAT
CTTTACCCGAAAGTGTTACTATTCACAAAAAATGA
TGTCAAAACCATCAAGTTCGGTTTTCGAGACTGTT
TTTATAGTTTGGTATGTAAATCGAAGT
[Bq25
AATTCCCATATAACCTCTTGAAGTTGAAGCTGAAT
TCTTCCTTATCCAAACAAAGGGAAATCATGGTGTA
TTTGTTTCCTAGTTATTGGATTTCTTTCCTGGTCAC
AGTCTGTGTCTAAAGATAGTGGGCAGGTCAAGG
GGTTTGGAGAGGGGTCTCGCTTGCGGCTATGGCT
GACGCTATCTTCTTCTTCTTCTTATTATTATTATTA
TTATTACATTTTGTTAATTAGGTTATTGAAAAAAC
CGGTAGGTGGTCCTTATCAGGTTGATCAAAAAGA
AAGAGGATGCGAGCTATTTTATCAGATCGCTGTC
ATTGGGCTCCATATGTGTTTTTTCTCAAACGTCTTA
AATTTGCCACAAGCCAAATGGGCGACAACAACTT
ATCAGGGTTTGT
[Bq26
GCCAGATATCNATACACATTTGTGAAATTTTTATA
CAATATAATTTAGCCATAAATGAATAAAGGCTGA
GAATTTCAACAACTGTGAAAATGGCAAAGTTGAC
ATGAAAATAAAAGAAGCCATCTCCAATTATAATT
TACTATTTTCTCCCATATTTCCACCAAATAATTCT
CTTAACAGACAACTCCTTTTTTTTCTTCCTCCTAGA
TGATTGAAGCTTACCTTTGATCGTCATATGCAAAT
TTGATGAAGAAAATAAAAGGGTAATTAGTAAGAA
ACTTGAAAAAAATTCGACATCGAAAACTTTACAT
GGTGATGAGATGATTGAATAATAATCTTCATCAG
GCACTCCTACATCTCCTCTTGAAACTGCGAGCTGA
TGACTCAACTGTAAATGGTTCAGCAGAGTTGGATC
GGAAAAACGACTCTACCTCTTCACTTCTTGTAGTT
TCGAAGAACTGCAACTCCGATAACTTCCTCTTGTG
CACGAAGCTTACTCTCACTTCCTTCTTGGGTGTCG
GTGGGCAGCTTTGGATGATGGGTATTTTGTGATCA
CTCGAAGTTGGCGTTCTGCAACCTTCTTTTTCTTCT
TCTTCGTCTTCTTCTTCTTTTTCAACACCTTCCCTC
GATGTCACACCTCCTTCTTGTTTTACCCCATCTTTT
TCAATCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT
CTTCTTCTTCTTCGTTTTGTTGTTCAAGTTTTTGTA
GACTTCCTTGAAGAATAACTTTGCCAATAGAATAG
ACACTAAACAGTGAGAAAAGTTGGAAACTAGTCA
AGGCATTGTAGCAGTTCTAAAATAAGATTGAGAG
AAAGATGTCCAATGTATTCTTGCACATCAAAGAA
GGCATCACTGGAATAAAACTCACTAGCCCAAGC
TCAAGGACACAAGTAACATGAAGCAACTTGGCCT
ATTAAAAGAAACCTAATACTTGGAGAATTCTAAA
GGGCCCAATTTGCTTGAAGTTTGTAAAAGCTTGGA
GATCCAATCTAATATAACTACTCCAATGT
[Bq27
ACAGATTCAGTTCAGTGAGATTCTCTCCACATCAC
AACTCCCTAATACAATTCAAGAAAACATGAAAAA
ACGAAAATTTAATCATACCAACTTTAAATTATAGC
AAGGCCAAGAGGATATCCAAAGACATCAATACAA
ACAAAGGGAATGTGTGTGTGTGTGTGTGTGTGTGT
GTGTGTGTGTATTTAAGCTGGTTGACGATAGAGCA
ATATTCCTGCGAAACCTCCACTAATCCCCTATGTT
ATGTTGATTAACTTCATAGATCATAAGAGGAAAA
[Bq29
ACCTTTGGCATAAAAGCATTCTGACTATTGCAATG
TGAAAATATCTCGGTAATCTTAAAGGACTTCTAAA
AAAGTGAACCTATCCTAAATATGTGTTTCTTCTTG
ATGCAAATGGGAGCCCCTTCTTTGTTTGTCTCACC
Conservation Genet Resour
123
AGTTTGATGAATTTTATGTGTTTGCTGTATTTTTA
TTTTTATGCTATTCATTTCCACAAGCCCTTTTTTT
TTGTTTGTCTCACCAGTAACCAGTTTGATGAATTT
TATGAGTTTGCTCTCTTTTTACTCGTGCATTATGC
TATTCTTTTCCATGAGTAAAATATGCAATTCTTTA
TGGTTTTGTCCTGACTGTAAGTATGACAAGAATAT
TAGATTGATAAATAAAGTTTGTTTCTGGAGCAAAA
GACTCTCTCTCTCTCTCTCTCTCTCTATCTCTCTCT
CTCTCTTTTGTGACTGTCGTCCTGTCCTGCAATGCT
TAATGCTTGCTTGATGTTAAAATGCATGTGTCTTT
TGATCTTGGTCATTTGTTTACACAACATAGGCTTG
GTTAAGTATGTTTTATTTTCAACTGAGTGTATAAA
GTTTTGATTGATGAAAATATTGGTTTTATTCATTC
AATTGACTTGTTTGATAACACTTTTGAATGGTTGC
AGGACTATCACTCAAATCCCAAGAACTAACAGCT
ATTTTTTTAGCT
[Bq30
ACACTCGTCCAGCAGCGATGACTTTGCTGCATTGC
TTGATGCTGAGCTCGAAGCCGGTTCTTCAGGCTCA
TCACCTGATGAACGAGACAATGAAGAGGAAGAAG
TTAATGCTGATGATGATGATGATGATGATGATGAT
GATGATGAAGATGAGGAGGATGATGTCCTTAACA
CTCAAAGGTGCATCTTCTAGGAATATTAAACTTTT
AATTTTGTTAGAGTTACTATCAAAGAAAAAAAAA
AATGAAGGGATGAACTTTTTTTAGTTTGCAAATGA
TTACAGGTGTGCACATTATTAACTTTGTGTTTTAA
TGGTCGTTGTGGACAGTTTTATTGTTTTATTTTTTT
ATGTTGTTCTAACAGTGTTTGTATATTTTCATCGTG
TTACATGAAACTATGCTAATTTTAAGATCCATGAC
TTTTTCGAAGAATACGAACAGCATATGCTTTTCCT
TGGTTGATTTCTTATTAATTTATGTTTTGGGTTTTG
AATTGGATGGATATGAGTTAATACATTGATAAATT
CGAGGAGATATTTAAAAATTTTTGGATTTTGTTCT
GCTTGATTTCAGAGTTGAGACCTACTGTAGTGATG
AATTTTTGTGGATATTTGGTTATGTTCTTGACAGA
ATTGAGGATTGATTATCCTTGGCATCTTATTTAAT
CATTTGACATTTGAATTGAACTTGCTGCTTTTATTT
CTTTTTAAACAAAAGGTTCTATAAATTTAACTTTT
AGGAGCAAAAGGTGCAAGACCGAGAAGTTGCAA
GACTTAGAAGAACCGCAAGGGTCAGCCTCTCAGA
GTTTGATAGAAGAAAAGTCAGGTAAAGTTCCTTA
ATATGGTTGTTTGCCCTTTTAGTTTATTTGAAACTT
GGATCTCTTTCACCCCCAAACTTCAGCACAATTAA
CTACCTGGAGACATGCTTAGAAATATCTCAACCTA
CAGATGACTTTTAGGAGATATAACAAGAGAGCAA
TTTGGCTGGAACTTATCTATTCTTAAACTTTAATC
TATTGCTGTTGATAAATGCTCAAAGAGGATGTGTC
TGTTTTAGATGGATGTGTGATNGCTGAANC
[Bq31
ACATAGATACATACCTCAGGTAGTTTCTGTTTGAA
AGCAACATCCAAGCGTGCATCTAAAGTATTCTCCA
AAACAATCTTCCCATCTTCTGAAGCCAGCACAACT
CCGCCCGAGCTAATTCATTTATTTTTCAGCATGTA
TGTATATGCATAAATTAATAAACCAAGCATTGCA
ATTAAACAAAATTAAAAGAAGGGTTAATTTTCGT
GTTACTTTTTTAATTTTTAATTATTAATTATATGGT
AAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
ATTACGCACCAGTAAGGTTGATGGGAATTATTAT
TATCTGGGTCAGTGGGAGGGGGTGGGAGGTAAAC
ATTGTCGATGGTAATCTTAGGTGGTGCTACATTG
AATCTCTCAGCGTAGACTCGTTTTGCATCTTCTA
AAACAGACTCAACCACCTTACGATCAACCTCTCT
GCACCTCAGTAACACTGCTGCCTCCCTCAGCCGA
ACCAGACTCTGCACCACCAATGCTTTGACCAGGT
TTTTGTATCCCCTCTTATCGTTACTCAGACGCAGC
AGCTCCTTCCTCGCACTTTCTTTTATGGAGTTCAC
CAGATCATCCTGTGCTTGCAGCACTTTTATCCTCG
ACGCGTTCAGCTGCATTGAGT
References
Alverson WS (1994) New species and combinations of Catostema
and Pachira (Bombacaceae) from Venezuelan Guayana. Novon
4:3–8
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci.
Molecular evolution: producing the biochemical data, part B.
Elsevier Academic Press, San Diego, pp 202–222
McPherson H, Porter C, Rymer PD, Crayn DM, Rossetto M (2008)
Isolation and characterization of polymorphic microsatellite loci from
Tetratheca ericifolia (Elaeocarpaceae). Mol Ecol Resour 8:867–869
Rossetto M, Crayn D, Ford A, Ridgeway P, Rymer P (2007) The
comparative study of range-wide genetic structure across related,
co-distributed rainforest trees reveals contrasting evolutionary
histories. Aust J Bot 55:416–424
Sandiford M (1998) A study of the reproductive biology of
Bombacopsis quinata (Jacq.) Dugand
Conservation Genet Resour
123