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ISOLATION R2 R1 FACS analysis CD44 + /CD24 -/low /lin - ALDEFLUOR assay Side population CD133? CD61? CD49f/CD29? MATERIAL Primary tumor Cell line Mouse xenograft PROPERTIES in vitro assay Tumorsphere formation Serial passages (self-renewal) in vivo assay R2 R1 Tumorigenicity assay Recapitulation of the initial phenotype Serial passages (self-renewal)

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MATERIAL. Primary tumor. Cell line. Mouse xenograft. CD44 + /CD24 -/low /lin - ALDEFLUOR assay Side population. CD133? CD61? CD49f/CD29?. R1. R1. R2. R2. ISOLATION. FACS analysis. in vitro assay. in vivo assay. PROPERTIES. Tumorigenicity assay - PowerPoint PPT Presentation

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Page 1: ISOLATION

ISOLATION

R2R1

FACS analysis

CD44+/CD24-/low/lin-

ALDEFLUOR assay

Side population

CD133?

CD61?

CD49f/CD29?

MATERIAL

Primary tumor Cell line Mouse xenograft

PROPERTIES

in vitro assay

Tumorsphere formation

Serial passages (self-renewal)

in vivo assay

R2R1

Tumorigenicity assay

Recapitulation of the initial phenotype

Serial passages (self-renewal)

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