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Page 1: ISOLATION

ISOLATION

R2R1

FACS analysis

CD44+/CD24-/low/lin-

ALDEFLUOR assay

Side population

CD133?

CD61?

CD49f/CD29?

MATERIAL

Primary tumor Cell line Mouse xenograft

PROPERTIES

in vitro assay

Tumorsphere formation

Serial passages (self-renewal)

in vivo assay

R2R1

Tumorigenicity assay

Recapitulation of the initial phenotype

Serial passages (self-renewal)

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