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UV Technologies as Alternative to Heat Pasteurization of Foods and Beverages: Is This a Reality? Tatiana Koutchma, PhD RadTech, San Francisco, March 1, 2017

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Page 1: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

UVTechnologiesasAlternativetoHeatPasteurizationofFoodsandBeverages:

IsThisaReality?

TatianaKoutchma,PhD

RadTech,SanFrancisco,March1,2017

Page 2: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

Outline• Introductioninto“Pasteurization”concept

• LegalstatusofUVforfoodsandbeverages

• UVasAlternativetoPasteurization– Juices

• UVasAdjuncttoPasteurization– Milk

• Conclusions

Page 3: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

FOODPRESERVATION

INHIBITION

CHEMICAL

pH,watercontentandwateractivity,preservatives,

PHYSICAL

Refrigeration,freezing,drying

INACTIVATION

STERILIZATION

Heat,Irradiation,PressureandHeat

PASTEURIZATION

Milderheat,irradiation,non-thermalphysical

methods

REMOVAL

Nano-filtration,Separation,

Centrifugation,Cleaning

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Page 4: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

FoodsCategories

4

Shelf-stable

pH>4.6

SterilizationAmbientStorage

(LAF)

PasteurizationRefrigeratedStorage

+GrowthinhibitionbarriersLAPF,ESL

Acid&Acidified

3.5<pH<4.6

Pasteurization,AmbientStorage+Growthbarriers,RefrigeratedFoods

(ESL)

HighAcid

pH<3.5

PasteurizationAmbientStorage

Page 5: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

PasteurizationConcept

• Applied for liquid foods

• Milk, beer, juices

• Eliminate the risk of most pathogenic bacteria

• Requires refrigerated storage

• Solids foods can be pasteurized in consumer packages to eliminate post-processing contaminants

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Page 6: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

Pasteurization

• 1862 - the first pasteurization test was completed by Louis Pasteur and Claude Bernard

• Term “pasteurization” was defined as a process of mild heat treatment to reduce significantly or kill the number of pathogenicand spoilage microorganisms

Milk (PMO)• 63° C for not less than 30 min, LTLT• 72° C for not less than 16 s, HTST• or equivalent destruction of pathogens and enzyme phosphatase

• Beer

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Page 7: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

Pasteurizationafter2004• Prior to 2002 FDA considered pasteurization as a thermal

treatment– FDA would not allow a nonthermal processing technology

to promote its treatment as a “pasteurization” process

• September 2004, the USDA National Advisory Committee on Microbiological Criteria for Foods (NACMCF) redefined the term pasteurization

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Any process, treatment, or combination thereof, that is applied to food to reduce the most microorganism(s) of public health significance to a level that is not likely to present a public health risk under normal conditions of distribution and storage

Food Chemical News, 2004

Page 8: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

2004NACMCF:AlternativePasteurizationTechnologies

• Cooking, steam and hot water treatments• Microwave processing (MW)• Ohmic/inductive heating• High pressure processing (HPP) • UV radiation, irradiation, light• Pulsed electric fields, pulsed light • Infrared processing • Non-thermal plasma • Oscillating magnetic fields • Ultrasound and filtration

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Page 9: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

EstablishmentofPreservationProcess1. Identification of the organism of concern for food category

2. Identification and selection of the appropriate target end point or specific log reduction (SLR)

3. Identification of critical factors

4. Development of a conservative estimation of the ability of the process to consistently deliver the target end point

5. Validation (microbiological or mathematically) of the lethal treatment delivered

6. Identification of procedures used to control the delivery of the required process.

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Page 10: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

ExamplesofSLRforfoodsatdifferentpHExamplesofProducts

pH PathogenofConcern

MicrobialReduction(Logs)

EnzymesDestruction

Applecider <3.5 E.coliO157:H7 5-log10 PPO

Orangejuice <3.5 Salmonella,E.coliO157:H7

5-log10 Pectinmethylesterase(PME)

Carrotjuice >4.6 non-proteolyticC.botulinum

5-log10

Milkandmilkproducts

~6.5-7 MycobacteriumtuberculosisCoxiella burnetii 5-log10

Negativeforalkalinephosphatase

Eggsproducts >7 SalmonellaenteritidisSalmonellatyphimurium

7-log10

In-shelleggs >7 Salmonella 5-log10RTEmeals >4.6 Listeria 5-7log10Almonds Salmonella 5-log10

Fishandseaproducts >4.6 non-proteolyticC.botulinum

6-log10

Crabmeat >4.6 TypeEnon-proteolyticC.botulinum

12-log10

Page 11: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

LegislativeStatusofUV• USA – UV irradiation is a food additive

FDA: CFR 21 179.39 UV radiation for the processing and treatment of food– Surface of food and food products– Potable water– Juice products

• Reduction of human pathogens and other microorganisms• Turbulent flow at Re >2000

– Bakingyeasts– Mushrooms– Milk The acceptance of UV as a food additive for dairy applications 21 CFR 179.39PMO submission

• Canada – UV light is a novel technology HC: Approval by Novel Foods Regulations

– Apple Cider, shelf-life extension • EU

EFSA: Approval by Novel Foods Regulations– Milk after pasteurization for shelf-life extension

• NZ and AustraliaFSANZ: Demonstrate equivalence to thermal treatment

Page 12: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

UVPreservation ProcessesForFluids• Shelf-lifeextensionofrawproducts

• Milk• Juiceproducts• Liquideggs

• AlternativetoHeatPasteurization• FruitandvegetableJuices(highacidoracid)• Milk• Coconutwater,coconutjuice(lowacid)

• AdjuncttoHeatPasteurization• Milk• LiquidEggs• LAdrinks(coffee)

Page 13: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

UVasAlternative toPasteurization

• Juices– highacidandacidpH<4.5

• Apple,orange,applecider– PathogenicE.coli,Listeriamonocytogenes,

Salmonella,Cryptosporidiumparvum

• Ingredients– Waterindairyprocessing– Liquidsweeteners

• Beer

Apple Juice

Page 14: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

UVPasteurization ofJuices

EstablishSpecificLogReduction

DesignReductionEquivalentDose(RED)

Criticalprocessandproductparameters

Validateforpertinentorganisms

pH>4.6– lowacidcategoryClostridiumbotulinum

pH<3.5– highacidcategory,pH<4.6acidic

PathogenicE.coli,Listeriamonocytogenes,Salmonella,Cryptosporidiumparvum

Validateagainst

Naturalmicro-flora,Enzymes

Shelf-lifestudies

QualityeffectsNutritionaleffects

vitamins,polyphenols,anti-oxidants

Sensorystudies

Page 15: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

IssuestoAddressforPasteurization

Microbiological• MostUVresistantpathogenorganism

– inmilk:18 pathogens– Mycobacteriumtuberculesis

• UVdosetoachieveSLRreductionofpathogens

• Surrogateorganisms• Validation• Verificationtests• Photo-reactivation

Chemical• Effectoncomposition• Cholesterol• Fattyacidsphoto-oxidation• Proteinoxidation• Furans• EffectsonNutrients(Vitamins)• Volatilecompounds• Off-flavors• Toxicologicalconcerns• Allergens

Page 16: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

CommercialUVsystemsPrinciple• Thinfilm

• DeanVortexflow

• Staticmixers

• Turbulentflow

Example• CiderCire,NYUSA

• Salcor Module,CA,USA• Uvivatec,Germany• AseptoRay,Israel

• SurePure,SA• MikroTec,UK• Aquafine/Trojan,Canada

• Atlantium,Israel• Aquionics,USA• AmericanUV• Ultradynamics,USA

Page 17: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

Mikrotec,UK

Surepure Inc,SA

ExamplesofUVsystems

CiderSure USAAseptoRay,Israel

Page 18: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

UVasAdjunct toPasteurizationofLAJuices

• Carrotjuice– absorptioncoefficientat254nm[cm-1]95,8± 0,3– turbidity[NTU]>11.000– viscosity[mPas]1,44± 0,19;density[gcm-3]1,037± 0,001– pH6,44± 0,01

• MaxRubner-Institut (Germany);DepartmentofFoodTechnologyandBioprocessEngineering:Heat+UVlight

• Heattreatment(65°C,5min)resultedina1-2logreductionoftotalaerobicmicrobialcount

• Additional3logreductionbyapplyingUV-C(4.3kJL-1)• Shelflifeofwasenhancedto12days• Minimalheattreatmentresultedinareductionofcarotenoids• NocarotenoidreductionwasobservedwhenUV-C(4.3kJL-1)wasapplied

Page 19: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

WhyAdjunct?

• UVlightisnoteffectiveagainstsporesasasinglealternativetreatment

• Difficultywithregulatorysubmissions– Datacollection

• BSL-2orBSL-3facility– Differencesinregulations

• NorthAmerica,EU,NZandAustralia,Japan– Newindicatorshavetobedeveloped

• Phosphatasetest

Page 20: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

UVasAdjunct toPasteurization• Sporesareorganismofconcern

• LowAcidjuicesandbeverages– Carrotjuice,coconutwater,icedcoffee– C.botullinum– Combinationwithmildheatcanberequired

• Extendedshelf-lifeproducts• Rawmilkorjuices• Acidjuices

– Alicyclobacillus cansurvivepasteurization– juices,icedteas

• ESLmilk– Heatresistantspoilagespores– UVisusedasapostpasteurizationtreatment

Page 21: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

UVTreatmentofMilk• Pre-pasteurizationtreatment

– Toextendshelf-lifeofrawmilk bycontrollinghighloadsofspoilageandpathogenicorganisms

– Non-thermalthermisation process

• Enhancesafetyandshelf-lifeofcheesemilk– Listeriamonocytogenesisaconcern

• Post-pasteurizationtreatment– Toextendshelf-lifeofpasteurizedmilk bycontrollingheatresistantspores

• Treatmentsofcalf milk

• Treatmentofhumandonormilk

Challenge:optimizingUVdosetoachievemicrobialreductionandpreventphoto-oxidationoflipidsandformationofundesiredchemicalcompounds

Page 22: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

UVResistanceofMilkPathogens

• Non-linearcharacterofmicrobialinactivation• ListeriamonocytogeneswasthemostUVresistantamong8milkpathogens• UVdosetoachieve5-loginactivationofListeriawasevaluatedas1936 J/l

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

0 10 20 30 40 50 60 70 80

Log(N)

Exposuretime[s]

x

SM

LM

EC

AH

WeibullmodelsFirst ordermodels

SMAHECLM

Page 23: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

UVInactivationofSporesinWholeUHTMilk

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Spore5-log

Reduction(J/L)

5-log Reduction

(J/cm2)R2 RMSE

Paenibacillus lautus >8800 >11.950 0.9952 0.0994

Bacillus cereus 7480 10.158 0.9960 0.1276Paenibacillusamylolyticus

5896 8.007 0.9928 0.2100

Bacillus pumilus 4752 6.453 0.9964 0.1717

Bacillus licheniformis 4048 5.497 0.9909 0.2426

Page 24: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

UVasAdjunct toRefrigerationandPasteurizationofMilk

Past.=18-21daysifdistributed&storedat4oC

ESL =60- 90+daysiffilled&packagedwithESLfiller&

distributedat4oC

Page 25: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

UVforShelf-LifeExtensionofRawMilk

• SurePure SP40UVunit

• UVdosesupto2.0kJ/lwereeffectivetoreducetotalviablecounts,psyhrophilesandcoliformsupto2,3and4-logreductions

• E.coliO157:H7,Salmonella,Yersinia,Staphylococcus,ListeriamonocytogenesandCampylobacterjejuni – 5-logreduction at2kJ/l

• Rawmilk- up14dayextensionofshelf-life

• Microbiologicalefficacyisachievedwithoutanydiscernabledenaturingoftheproduct`sconsistency,color,flavororaroma

Page 26: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

UVforPasteurisedMilkEFSAscientificopinion:January2016

• Thenovelfoodiscow’smilk(whole,semi-skimmedor skimmed)towhichatreatmentwithultraviolet(UV)radiationisappliedafterpasteurisationinorder toextendtheshelflifeofthemilk.ThistreatmentresultsinanincreaseinthevitaminD3 concentrations.

• ThePanelconsidersthattheprovidedcompositionaldata,thespecificationsandthe datafrombatchtestingdonotgiverisetosafetyconcerns.

• Thedataprovidedontheproduction processaresufficientanddonotgiverisetosafetyconcerns.

Page 27: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

EfficacyofUVLightforMilk• UVtreatmentofrawmilkcanachivea3to4log10 reductionofinitial

microbialloadmeasuredasstandardplate,psychrotrophic,coliformandthermoduriccounts– Extendshelflifeofrawmilkupto14days

• UVwasfoundeffectiveagainstpathogenicbacteriafoundinmilk.AreductioninEscherichiacoliO157:H7,Listeriamonocytogenes,Salmonellasenftenberg,YersiniaenterocoliticaandStaphylococcusaureus canbeachieved– Ensurethesafetyofmilk

• UVlightcandestroyspoilagesporeformersinmilk– Extendshelfofpasteurizedmilk(ESL)

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Page 28: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

Conclusions• Alternativenon-thermaltechnologiesincludingUVlightare

availableasadjunctstopasteurizationandESLprocesses– Canofferenergysavings,betterquality,longershelflifeduring

transportation

• Asanon-thermalandnon-chemicalpreservationUVlight canbeusedformilkandjuiceproductsas– adjuncttorefrigeration– pasteurization– ESLmethod

• Regulatoryapprovalisahurdleforcommercialization

Page 29: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

ReleasedbyElsevier

http://store.elsevier.com/authorDetails.jsp?authorId=ELS_1151578&locale=en_EU

Page 30: Is This a Reality?...level that is not likely to present a public health risk under normal conditions of distribution and storage Food Chemical News, 2004. 2004 NACMCF: Alternative

Questions?

Dr.TatianaKoutchma

Contactinfo: [email protected]