IP GROUP PLC WORK PLACEMENT

IP GROUP PLC WORK PLACEMENT ON 3D BIO PRINTING By Andres Barco

In simple terms it is the use of computer aided transfer process (3D printer) for patterning and assembling living and non living materials with a predefined 3D organization in order to produce bio-engineered structures that can be used in regenerative medicine and tissue engineering

What is 3D Bioprinting ?

Conceptual Bio printer

BUT How?

Bio printing represent a big paradigm shift when compared to traditional approaches in tissue engineering. Correct positioning of cell within a scaffold.Layer by layer approach allows for precise organisation of individual elements of the tissue.Step towards off the shelf organs/tissues for transplantation and drug testing.

Bio-printing vs. Tissue Engineering

The IP gorup life sciences team identified 3D tissue/bio printing as an area with significant potential to disrupt the healthcare market in the near future.

Therefore a 2 week placement targeted to inform IP group investment strategy and due diligence in this sector was undertaken.

How did my placement come about ?

Map the technology space Who are the leaders in the field ? Academic groups, corporate R&D, spin outs, companies, etc.Who are the key people ? Area of expertise ?Collaborations ?How mature is each technology area ? Building blocks to commercialise the tech if any ?Technical challenges arisen ? What application are being targeted ?Tissue/ organs ? Now & future ? Any overlaps or obvious synergies in whats out there and current investments that IP group already have ? What's missing ?

Aims of the mini project ?

Basis of assessment of individual companies and research institutes that are involved in this field. The Tech building blocks where divided into three processes that occur within most 3D bio-priting processes:Pre-processing/ InputsProcessing/ PrintingPost-processing/ OutputsApproach Identifying the Technology Building Blocks

InputCellsLarge amounts need to be cultured for bio-inkViability pre-printingMaturation time needs to be known beforehand.Scaffold/support materialStructural support to cells but has to be non-adherent.Biocompatible and easy to remove or biodegradable.CAD model/Blue print High resolution of imaging. Easy of translation to printable modelMapping of multiple cell types.

Fundamental Tech Building Blocks that need to be considered:

Printing Resolution Micro/nano level accuracy FastPrevents cellular differentiation from normal phenotype or worse cell death. ApproachMechanical forces of individual printing techniques ?Adverse effects ?VasculatureProvides cells with correct nutritionOutputMaturation Culture time before its ready for implantationAnalysis of whether it behaves the way it should ?

Tech Building Blocks continuted

Research groups and companies were ranked from 1-5 (5 being the best) in each of the following categories: CapacitySize of groupPapers publishedGrants/ collaborationsReadinessWhat stage are they at with idea/research/productData produced / experiments Clinical trials ? Technology coolnessMy subjective opinion on how novel their idea/research/approach is and whether it is commercially viable. Ranking System

Capacity x Rediness x Coolness = Overall

3D functional organ/tissueLithography based techniques2-photon polymerisation (2PP) Normal approach (stereo)University of Vienna3D CultureBio printing 3D gels Rice University, Institute AMRISydney University, DentistryProjection approach (mask image based)University of California, San Diego (UCSD).Magnetic LevitationOtherBeadsRainbow Biosciences + n3D biosciences (company) Cronim 3D(company)University of Tokoyo, Institue of industiral sciencesCell ApproachesNo Cells DropletsVesicleNo ScaffoldScaffoldUniversity of Manchester, Derby groupOxybio (company)Layer by layerDropletsInkjet approachLaser assistedLaser induced forward transferLaser guided direct writing University of Bordeaux, Bioeng dept, TEAL groupSolid ScaffoldSoft ScaffoldRigid ScaffoldNeedle arrayOtherPorous ScaffoldCell In-growth OtherNon PorousDirect cell printingCyfuse Biomedical (company)University of Manchester, Derby groupUniversity of Sydney, Eng & info technologyUniversity of Korea (POSTECH)Multiple Cells Single CellUniversity of Edinborough, Inst of Bio chemistry, biophysics and bioengineering1) University of MIT, Regen Medicine2) Skin Print (company)5) University of Ediborough 6) University of Tokyo - Matsunga GroupRanking system (overall) :1-34 = potential but early stage25-68 = Ones to look out for69 100 = Ones likely to excel 3) University of Vienna, Dept of additive manufacturingOrganovo (company)2) Wake forest University Regen Med - AFIRM3) Rice University Inst of AMRI4) Harvard University WYSS Institute Spheriods

Organovo What they do ?

Most advanced Regenerative Medicine centres in the world. Working on 3D printing of:Kidneys LiverBlood vesselsSkin Collaborating with Armed Forces Institute of Regenerative MedicineSkin regen for burns victimFacial, skull, genital and Urinary reconstructions

Wake Forest University

Developing laser and UV light based 3D bioprinting techniques, including laser direct ablation, UV nanoimprinting, and dynamic optical projection stereolithography (DOPsL) for biomaterials.

University of California, San Diego

The biofabrication technique uses a computer projection system and precisely controlled micromirrors to shine light on a selected area of a solution containing photo-sensitive biopolymers and cells. This photo-induced solidification process forms one layer of solid structure at a time, but in a continuous fashion.15

Gained interesting information on 3D biopritning world. Experience working within a life sciences team, whom have many different portfolios.Complying huge amounts of information into small pieces of digestible information to relay on. Working with complete freedom to tackle a problem on my own. Reflective writing with Critical analysis.

Summary of what was learnt

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And . That im pretty good at 5Ks. Summary of what was learnt

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THANKS FOR LISTENING !