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Invader Assay

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We have got the map (genomic sequence) to find treasure so that we can get

treasure chest.

Key to open the chest（Post genomic

technology)

Getting treasure (new drugs)!!

Even if we get the treasure chest (target gene), we can’t open it (because we can’t access to its function

in disease.)

Current genomic researches have tried pulling out of all nails on the chest.However, the number of the nails may be infiinite…

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What is the difference between human & ape? Genomic research

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Progress of Researchgenome Genomic sequence ・ Polymorphism

（ SNP etc （

Transcriptome Gene transcription profile

Proteome Expression profile of proteins

Functional proteomeMetabolome

Genetic functionPost translational modification

Protein interation etc.Time consuming and

enormous costElucidation of functional network of cellular molecules　 　 　 　 　

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Technologies in each categories

Genome

Structure analysis(sequence)

Polymorphism analysis

-DNA chip 　 -Invader assay-Sniper assay 　 -PROBE assay

-Luminex 　　　 -PCR-SSCPPCR-RFLP etc-

Transcriptome・ Differential expression analysis

・cDNA chip etc

ProteomeIdentification

Protein function 　Protein interactionLigand interaction

Post translational modification

・protein chip, peptide chip・Y2H・SELEX・Phage display・STABLE assay etc

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Used for

The detection of single nucleotide polymorphisms (SNPs)

Detection of mutations that confer resistance to antiviral agents

Genotyping of isolates for epidemiological purposes; categorizes patient isolates into 8 different HBV genotypes (A to H) and 6 different HCV genotypes (1 to 6 with 24 subtypes)

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T

N

C

G A

Reporter probes Flap FlapInveder probesN

C T

GN N

A

Cleavage Cleavage

Endoflap Nuclease

Fluorophore 1 Fluorophore 2Quencher Quencher

C

Cleavage

T

Invader Assay

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Advantage: PCR is unnecessary

Drawback: Quite large amount of sample is required

Background reaction exists